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The map label for this gene is purR [H]

Identifier: 89053915

GI number: 89053915

Start: 1393637

End: 1394635

Strand: Reverse

Name: purR [H]

Synonym: Jann_1424

Alternate gene names: 89053915

Gene position: 1394635-1393637 (Counterclockwise)

Preceding gene: 89053922

Following gene: 89053911

Centisome position: 32.3

GC content: 63.26

Gene sequence:

>999_bases
ATGGCCGTAACACTCAAGGATGTGGCGGAACGCGCGGGCGTCTCGACCTCCGCCGTGTCGCGCACCTTCACCGAAGGGGC
GTCCGTCTCGCCCAAGACCCGCGCCAAGGTGGAGCGCGCCGCCGATGCGCTGGGATATTCCCCCAATGCGCTGGCCTCCT
CGCTGACCACGGGGCGCACGAAATTGATTGGGCTGGTCTCCAACAACTTCCACAACCCTCTGTTTTTGGAGGTTTTTGAC
AGGTTCACGCGGGGATTGCAGGACCGCGGCCTGCGCCCGCTTCTAGTCAACCTGTCCGATGAGACGGACCCGATCAGCTC
CGTCCGGATGATGCGGCAATATTCCGTCGATGGCGCGATTATCGCCTCCTCCACCCTGCCGCCAAGTTTTGCGGAGGCCT
ACCGGATGGCGGGCATCCCCGTCGTCCATTCCTTCGGCCGCTACACCACCGCGCCCCAGGTTCACGTCGTGGGTATCGAC
AATGTCGCCTGTGGGCGAATGGCGGGAGAAGCCCTGATTTCAAGAGGTTACAGGCGCGTCGCGTTCCTCGGCGGTCCAGA
GGGGGCAACGTCGACGCAGGATCGCGCGCTTGGGTTTCTGGGGGCCTTGCACGAACACCCCGAGATTGAGGTGACCGTCA
GCTATGCGCAGGCCTATTCCTTCGACGCGGGCCGGGTCGAGATGACCAAGCTCCTGATGTCCGACCCAGCGGAGGCCTAC
TTTTGCGGTGATGATGTTCTATCGGTCGGCGCTTTGTCGGCGGTGCAGGAGGCCGGGCTATCCGTGCCCGGCGACATCGG
CATCATCGGGCTCAACGACATGCAGATGGCGGGCTGGCAGAATATTAACCTTACCACGATCCGCCAACCGGTGGCCGAGA
TCATCGGCGCGTCGATTGATTTGGTGATCGGCACCGTGGAGGATCCGAAGCGCCATCCCGAGACGCGGCTATTCCCCTGC
CGCGTGATCGAACGGGGCACCTTGCGGAAGGTGCCTTAG

Upstream 100 bases:

>100_bases
GACTATTTTGCAACCGGTTGCAAAAATTGCAAACAATGGTCAGATTGGGTCCTGTTTTGCACCCGATGCAGGCGACTGAC
GCGAGGAAGGGCAAGGGTTA

Downstream 100 bases:

>100_bases
CGCACGCAATGTCCGATTTTTAGTAACAGTGTGCGCATACGGTGTAGTTACAGAAAAACTACAGCTTCACTACAGGATCT
TTACACCTGCTCAGCGGAAG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAVTLKDVAERAGVSTSAVSRTFTEGASVSPKTRAKVERAADALGYSPNALASSLTTGRTKLIGLVSNNFHNPLFLEVFD
RFTRGLQDRGLRPLLVNLSDETDPISSVRMMRQYSVDGAIIASSTLPPSFAEAYRMAGIPVVHSFGRYTTAPQVHVVGID
NVACGRMAGEALISRGYRRVAFLGGPEGATSTQDRALGFLGALHEHPEIEVTVSYAQAYSFDAGRVEMTKLLMSDPAEAY
FCGDDVLSVGALSAVQEAGLSVPGDIGIIGLNDMQMAGWQNINLTTIRQPVAEIIGASIDLVIGTVEDPKRHPETRLFPC
RVIERGTLRKVP

Sequences:

>Translated_332_residues
MAVTLKDVAERAGVSTSAVSRTFTEGASVSPKTRAKVERAADALGYSPNALASSLTTGRTKLIGLVSNNFHNPLFLEVFD
RFTRGLQDRGLRPLLVNLSDETDPISSVRMMRQYSVDGAIIASSTLPPSFAEAYRMAGIPVVHSFGRYTTAPQVHVVGID
NVACGRMAGEALISRGYRRVAFLGGPEGATSTQDRALGFLGALHEHPEIEVTVSYAQAYSFDAGRVEMTKLLMSDPAEAY
FCGDDVLSVGALSAVQEAGLSVPGDIGIIGLNDMQMAGWQNINLTTIRQPVAEIIGASIDLVIGTVEDPKRHPETRLFPC
RVIERGTLRKVP
>Mature_331_residues
AVTLKDVAERAGVSTSAVSRTFTEGASVSPKTRAKVERAADALGYSPNALASSLTTGRTKLIGLVSNNFHNPLFLEVFDR
FTRGLQDRGLRPLLVNLSDETDPISSVRMMRQYSVDGAIIASSTLPPSFAEAYRMAGIPVVHSFGRYTTAPQVHVVGIDN
VACGRMAGEALISRGYRRVAFLGGPEGATSTQDRALGFLGALHEHPEIEVTVSYAQAYSFDAGRVEMTKLLMSDPAEAYF
CGDDVLSVGALSAVQEAGLSVPGDIGIIGLNDMQMAGWQNINLTTIRQPVAEIIGASIDLVIGTVEDPKRHPETRLFPCR
VIERGTLRKVP

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=29.3768545994065, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=32.7433628318584, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=32.1428571428571, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=28.5294117647059, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI48994940, Length=290, Percent_Identity=27.9310344827586, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=26.027397260274, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787580, Length=288, Percent_Identity=28.4722222222222, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=25.7309941520468, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=27.1293375394322, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=26.2048192771084, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=22.7848101265823, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790192, Length=220, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35580; Mature: 35449

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTLKDVAERAGVSTSAVSRTFTEGASVSPKTRAKVERAADALGYSPNALASSLTTGRT
CEEEHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCE
KLIGLVSNNFHNPLFLEVFDRFTRGLQDRGLRPLLVNLSDETDPISSVRMMRQYSVDGAI
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEE
IASSTLPPSFAEAYRMAGIPVVHSFGRYTTAPQVHVVGIDNVACGRMAGEALISRGYRRV
EECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEE
AFLGGPEGATSTQDRALGFLGALHEHPEIEVTVSYAQAYSFDAGRVEMTKLLMSDPAEAY
EEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCEE
FCGDDVLSVGALSAVQEAGLSVPGDIGIIGLNDMQMAGWQNINLTTIRQPVAEIIGASID
EECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCHHHHHHHHHHHHCCCEE
LVIGTVEDPKRHPETRLFPCRVIERGTLRKVP
EEEECCCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AVTLKDVAERAGVSTSAVSRTFTEGASVSPKTRAKVERAADALGYSPNALASSLTTGRT
EEEHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCE
KLIGLVSNNFHNPLFLEVFDRFTRGLQDRGLRPLLVNLSDETDPISSVRMMRQYSVDGAI
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEE
IASSTLPPSFAEAYRMAGIPVVHSFGRYTTAPQVHVVGIDNVACGRMAGEALISRGYRRV
EECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEE
AFLGGPEGATSTQDRALGFLGALHEHPEIEVTVSYAQAYSFDAGRVEMTKLLMSDPAEAY
EEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCEE
FCGDDVLSVGALSAVQEAGLSVPGDIGIIGLNDMQMAGWQNINLTTIRQPVAEIIGASID
EECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCCCCCHHHHHHHHHHHHCCCEE
LVIGTVEDPKRHPETRLFPCRVIERGTLRKVP
EEEECCCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA