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The map label for this gene is iolD [H]

Identifier: 89053911

GI number: 89053911

Start: 1388400

End: 1390271

Strand: Reverse

Name: iolD [H]

Synonym: Jann_1420

Alternate gene names: 89053911

Gene position: 1390271-1388400 (Counterclockwise)

Preceding gene: 89053915

Following gene: 89053910

Centisome position: 32.2

GC content: 58.49

Gene sequence:

>1872_bases
ATGACCGAACCCACATCTACCATTCGGCTGACGGTAGCCCAGGCCATTGTGCGTTACCTCATGAATCAGTTCATCGAGAT
TGATGGCGTCGAGACCCGGATCTGCGGAGGCGGATTCGGCATTTTCGGGCACGGCAACGTGCCATGCCTGGGGGAAGCGC
TTTACCCTGTGCAGGACGAGATGCCGCTTTACCGTGGGCAGAACGAGCAAAGTATGGGGTTTGCCGCCGCCGCTTACGCG
AAATATCATCTGCGTCGCCGCTTCATGTTTTGCACGGCATCAGCCGGGCCCGGAACGGCAAACCTGCTTACGGCATCTGC
CTTGGCGCACGCAAATCGCTTGCCATTGCTGATGTTGTGCGGCGATACCTTTCTGACCCGTCTGCCCGATCCGGTCCTGC
AACAACTGGAGCACTTCGGAAACCCAACGCTTGGCGTGAACGATGCATTCAAGGCCGTGACGCGCTTCTGGGATCGAATC
ACGCATCCCGCACAGCTGCTGAATGCGCTACCTGCCGCCCTTGCGACGATGCTGGACCCTGCCGATTGCGGGCCCGCGTT
CCTCGGATTGCCGCAAGATGTCCAGGGGTGGGCGTATGATTATCCCGAGGTCTTCTTCGCCCGCCATGTGCATCGCATCC
GGCGACAAGCACCTGATCCGGCTGAGGTTTTTGATGCCGCAGCCCTTTTGGTTAACGCAAGGCGACCCGTGATCATCGCG
GGCGGCGGCGTGCAATATGCTGGCGCAGTGGACGCGCTGACCCAATTTGCCGACACACACAACATTCCCGTGGTGGAGAC
CATCGCAGGCCGCGCTAACATGGTGGCAACAAATCCGTTAAATATCGGGCCCTTGGGTGTAACCGGGTCAGATTCAGCCA
ACGCCATCGCCGCCGAGGCCGATGTGATTTTGGCCGTTGGCACACGGCTTCAGGACTTCACAACAGGTTCATGGACAGCG
TTCGCCCATGATGCGCAGATCATCGGCATGAATGTTGGCCGTCATGATGCAGCAAAACATCTGTCTTTACCGGTCGTTGG
CTGTGCAAAACTCAGCCTCCCCGCATTAAGCGCGGCACTGTCAGACTACTCCGCGCCCGAGGCCTGGATGACAAAAGCGC
AGGCAGGACGCGCAAGTTGGGACGCTTACGTGGTCGAGAACGTGGCGCACGGGAACCGCCCTAATTCCTACGCGCAGGCC
ATCGGTGTGGTGAATGCGCTTTGCGACACACGGGACCGCGTTGTCACGGCTGCGGGAGGGCTTCCGGCCGAGGTCACTGC
CAACTGGCGAACGCTCGACATTGGCACCGTCGATGTGGAGTTCGGTTTCTCCTGTATGGGCTATGAGATCGCTGGTGGAT
GGGGTGCAAAAATTGCACAATCCGAGCAAGAGCCCACAGCGGATACCATTGTTTTCGTCGGAGACGGTAGCTATCTCTTG
ATGAATTCCGATATCTACTCAAGCGTTCTGACTCGGAAGAAACTTATCGTTCTGGTCCTCGACAACGGTGGTTTTGCGGT
CATCAACAAGCTGCAAAACAATACCGGAAACGAGAGTTTCAACAACCTTATCGCGGATTGCCCCACTATACCGGAGCCGT
TCACGGTCGACTTCGAAGCTCACGCGCGCGCCATGGGGGCGCATGCGGAAACCGTGTCCAACCCAGCAGAACTGGCAGAT
GCATTCCAGCGGGCGAAGACGGCGGACAGGACTTCTGTCATCGTCATGAAGGTCGACCCCTATGACGGCTGGACCACCGA
AGGCCACACATGGTGGGAAGTTGGGACGGCCCAGGTGTCCGACAACCCGAATGTCCGCGAAAAGCATGCGGAATGGGAAG
CGGACCGTAACAAGCAGCGACAGGGCGTGTGA

Upstream 100 bases:

>100_bases
TCGTGTTGCAACCGCTTGCAAAACACGTATCCTGCCCTCAAGAGCTTCGCAATATCCAATCTGAGCGACATCGTTAACGG
GCACGGACCGGGGAATGATC

Downstream 100 bases:

>100_bases
TGAAGTTGCGCGGCAATAATTTCGTGATCATTGGCCGGGCTGGCATGGATCTCTACCCGGATCCTCCCGGCACACGGACC
GAAGATGCGACCCACTTCTT

Product: thiamine pyrophosphate enzyme protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTEPTSTIRLTVAQAIVRYLMNQFIEIDGVETRICGGGFGIFGHGNVPCLGEALYPVQDEMPLYRGQNEQSMGFAAAAYA
KYHLRRRFMFCTASAGPGTANLLTASALAHANRLPLLMLCGDTFLTRLPDPVLQQLEHFGNPTLGVNDAFKAVTRFWDRI
THPAQLLNALPAALATMLDPADCGPAFLGLPQDVQGWAYDYPEVFFARHVHRIRRQAPDPAEVFDAAALLVNARRPVIIA
GGGVQYAGAVDALTQFADTHNIPVVETIAGRANMVATNPLNIGPLGVTGSDSANAIAAEADVILAVGTRLQDFTTGSWTA
FAHDAQIIGMNVGRHDAAKHLSLPVVGCAKLSLPALSAALSDYSAPEAWMTKAQAGRASWDAYVVENVAHGNRPNSYAQA
IGVVNALCDTRDRVVTAAGGLPAEVTANWRTLDIGTVDVEFGFSCMGYEIAGGWGAKIAQSEQEPTADTIVFVGDGSYLL
MNSDIYSSVLTRKKLIVLVLDNGGFAVINKLQNNTGNESFNNLIADCPTIPEPFTVDFEAHARAMGAHAETVSNPAELAD
AFQRAKTADRTSVIVMKVDPYDGWTTEGHTWWEVGTAQVSDNPNVREKHAEWEADRNKQRQGV

Sequences:

>Translated_623_residues
MTEPTSTIRLTVAQAIVRYLMNQFIEIDGVETRICGGGFGIFGHGNVPCLGEALYPVQDEMPLYRGQNEQSMGFAAAAYA
KYHLRRRFMFCTASAGPGTANLLTASALAHANRLPLLMLCGDTFLTRLPDPVLQQLEHFGNPTLGVNDAFKAVTRFWDRI
THPAQLLNALPAALATMLDPADCGPAFLGLPQDVQGWAYDYPEVFFARHVHRIRRQAPDPAEVFDAAALLVNARRPVIIA
GGGVQYAGAVDALTQFADTHNIPVVETIAGRANMVATNPLNIGPLGVTGSDSANAIAAEADVILAVGTRLQDFTTGSWTA
FAHDAQIIGMNVGRHDAAKHLSLPVVGCAKLSLPALSAALSDYSAPEAWMTKAQAGRASWDAYVVENVAHGNRPNSYAQA
IGVVNALCDTRDRVVTAAGGLPAEVTANWRTLDIGTVDVEFGFSCMGYEIAGGWGAKIAQSEQEPTADTIVFVGDGSYLL
MNSDIYSSVLTRKKLIVLVLDNGGFAVINKLQNNTGNESFNNLIADCPTIPEPFTVDFEAHARAMGAHAETVSNPAELAD
AFQRAKTADRTSVIVMKVDPYDGWTTEGHTWWEVGTAQVSDNPNVREKHAEWEADRNKQRQGV
>Mature_622_residues
TEPTSTIRLTVAQAIVRYLMNQFIEIDGVETRICGGGFGIFGHGNVPCLGEALYPVQDEMPLYRGQNEQSMGFAAAAYAK
YHLRRRFMFCTASAGPGTANLLTASALAHANRLPLLMLCGDTFLTRLPDPVLQQLEHFGNPTLGVNDAFKAVTRFWDRIT
HPAQLLNALPAALATMLDPADCGPAFLGLPQDVQGWAYDYPEVFFARHVHRIRRQAPDPAEVFDAAALLVNARRPVIIAG
GGVQYAGAVDALTQFADTHNIPVVETIAGRANMVATNPLNIGPLGVTGSDSANAIAAEADVILAVGTRLQDFTTGSWTAF
AHDAQIIGMNVGRHDAAKHLSLPVVGCAKLSLPALSAALSDYSAPEAWMTKAQAGRASWDAYVVENVAHGNRPNSYAQAI
GVVNALCDTRDRVVTAAGGLPAEVTANWRTLDIGTVDVEFGFSCMGYEIAGGWGAKIAQSEQEPTADTIVFVGDGSYLLM
NSDIYSSVLTRKKLIVLVLDNGGFAVINKLQNNTGNESFNNLIADCPTIPEPFTVDFEAHARAMGAHAETVSNPAELADA
FQRAKTADRTSVIVMKVDPYDGWTTEGHTWWEVGTAQVSDNPNVREKHAEWEADRNKQRQGV

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=523, Percent_Identity=24.0917782026769, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI87081685, Length=525, Percent_Identity=24.7619047619048, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=21.996303142329, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786717, Length=270, Percent_Identity=26.2962962962963, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787096, Length=504, Percent_Identity=22.6190476190476, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=21.8637992831541, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 66963; Mature: 66832

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTSTIRLTVAQAIVRYLMNQFIEIDGVETRICGGGFGIFGHGNVPCLGEALYPVQDE
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC
MPLYRGQNEQSMGFAAAAYAKYHLRRRFMFCTASAGPGTANLLTASALAHANRLPLLMLC
CCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
GDTFLTRLPDPVLQQLEHFGNPTLGVNDAFKAVTRFWDRITHPAQLLNALPAALATMLDP
CCHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ADCGPAFLGLPQDVQGWAYDYPEVFFARHVHRIRRQAPDPAEVFDAAALLVNARRPVIIA
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
GGGVQYAGAVDALTQFADTHNIPVVETIAGRANMVATNPLNIGPLGVTGSDSANAIAAEA
CCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEECC
DVILAVGTRLQDFTTGSWTAFAHDAQIIGMNVGRHDAAKHLSLPVVGCAKLSLPALSAAL
CEEEEECCCHHCCCCCCEEEEECCCEEEEEECCCHHHHHHCCCCEEEHHHHHHHHHHHHH
SDYSAPEAWMTKAQAGRASWDAYVVENVAHGNRPNSYAQAIGVVNALCDTRDRVVTAAGG
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
LPAEVTANWRTLDIGTVDVEFGFSCMGYEIAGGWGAKIAQSEQEPTADTIVFVGDGSYLL
CCCEEECCEEEEEEEEEEEECCCEEECEEEECCCCCHHCCCCCCCCCCEEEEEECCCEEE
MNSDIYSSVLTRKKLIVLVLDNGGFAVINKLQNNTGNESFNNLIADCPTIPEPFTVDFEA
ECCHHHHHHHCCCEEEEEEECCCCEEEEEEHHCCCCCHHHHHHHHCCCCCCCCEEEEHHH
HARAMGAHAETVSNPAELADAFQRAKTADRTSVIVMKVDPYDGWTTEGHTWWEVGTAQVS
HHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEEC
DNPNVREKHAEWEADRNKQRQGV
CCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEPTSTIRLTVAQAIVRYLMNQFIEIDGVETRICGGGFGIFGHGNVPCLGEALYPVQDE
CCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC
MPLYRGQNEQSMGFAAAAYAKYHLRRRFMFCTASAGPGTANLLTASALAHANRLPLLMLC
CCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEE
GDTFLTRLPDPVLQQLEHFGNPTLGVNDAFKAVTRFWDRITHPAQLLNALPAALATMLDP
CCHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
ADCGPAFLGLPQDVQGWAYDYPEVFFARHVHRIRRQAPDPAEVFDAAALLVNARRPVIIA
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
GGGVQYAGAVDALTQFADTHNIPVVETIAGRANMVATNPLNIGPLGVTGSDSANAIAAEA
CCCEEEHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEECC
DVILAVGTRLQDFTTGSWTAFAHDAQIIGMNVGRHDAAKHLSLPVVGCAKLSLPALSAAL
CEEEEECCCHHCCCCCCEEEEECCCEEEEEECCCHHHHHHCCCCEEEHHHHHHHHHHHHH
SDYSAPEAWMTKAQAGRASWDAYVVENVAHGNRPNSYAQAIGVVNALCDTRDRVVTAAGG
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
LPAEVTANWRTLDIGTVDVEFGFSCMGYEIAGGWGAKIAQSEQEPTADTIVFVGDGSYLL
CCCEEECCEEEEEEEEEEEECCCEEECEEEECCCCCHHCCCCCCCCCCEEEEEECCCEEE
MNSDIYSSVLTRKKLIVLVLDNGGFAVINKLQNNTGNESFNNLIADCPTIPEPFTVDFEA
ECCHHHHHHHCCCEEEEEEECCCCEEEEEEHHCCCCCHHHHHHHHCCCCCCCCEEEEHHH
HARAMGAHAETVSNPAELADAFQRAKTADRTSVIVMKVDPYDGWTTEGHTWWEVGTAQVS
HHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEEC
DNPNVREKHAEWEADRNKQRQGV
CCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA