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The map label for this gene is potB [H]

Identifier: 89053760

GI number: 89053760

Start: 1229302

End: 1230975

Strand: Direct

Name: potB [H]

Synonym: Jann_1269

Alternate gene names: 89053760

Gene position: 1229302-1230975 (Clockwise)

Preceding gene: 89053759

Following gene: 89053761

Centisome position: 28.47

GC content: 61.35

Gene sequence:

>1674_bases
ATGTCCGACGCCACCGGCCCCGACCAGTTCACCGCACCCACACCCGACAACGCCCTGCGCAATGCCGAGCGCACCGATAC
CGGCCCGGTCCTTGCCGCCGATGGAACACCCCTGAAGCGCAGCCTTTCGAAGGCCTTGCGGGCGCAGAAGCTGCGCGCGC
TGATGCTGATCGCGCCGCTGCTGATTTTCATCATGGTCGCCTTCATCGCGCCCATCGTCGATATGCTCTTCCGCTCCGTC
GAGAACCAGATCACGTCCGAGACGCTGCCGCGCACCGTTGTGGCGTTGGAAAACTGGGACCCGGAAGGCCTGCCCGACGA
CGACACCTATGCCGCGCTGACCTATGATTTTGTCGAAGCGGTGGAGCGACGCATCCACACACGCCTTGGCTCTCGCCTGA
ATTACGAGACGACGGGAATGTCGTCGCTGTTTCGCCGCTCGGGCCGGGGGGCCGATGATATTGGCGAAGACTATGCCGAC
CAGTTTCACGACCTGAACCCCGCCTGGGAAGAGGCCGCCACATGGGCATCCCTGATGGGCGCGCCCGATTGGCTTGAGGA
TGCAAGCGCTGCGGACGGCGCGGGCCTGCCATTCCGCCTGTCTGTTGCCGCGATGGAAACCCTGCCCGAGACCGCAGACG
CTTACCGCAGTTTCGCGCGGACCGTGCAGCAGGAAGACAACGACAACCCCACGGCGGAAGAGCCCTGGAACTCCGTCTAC
CTGGCCCTTTACAGGGACCTGAGCCGCGTCGATGTGTCAGGCTATGACGGACCGCAAGCTGATCTGTTGGCCGAGGCGGC
AGCCGCTGTAGATGGATTTGAAGGCCTGTCCCCGCGGGAGGCTTGGCTGGAGGTGAACGAGGATTGGGGCGATGTCGACG
TTTGGGCCACGATTGAAGCGTTCTCGGACCCCTACACCGCTGGATATTTCCTGACGTCGATTGACCTGCAACTGACCCCT
CAGGGCATCGAATCCCAGCCGGAAGAGCAGCAAATCTATGTGCTGTTGTTCCAGCGCACGATCTTCATGTCGGGCATGAT
TACACTGATGTGCGTGCTCTTGGGCTACCCGATTGCCTACCTGCTGTCGAACCTGCCGTCGAAGTCGTCCAACCTGCTGC
TGATCCTTGTTTTGCTGCCCTTCTGGACGTCACTTCTGGTGCGGACCAGCGCGTGGAAAGTGCTTCTGCAACAACAAGGT
GTGATCAACGATATCCTTGTGTGGGTCGGTATTGTGGCCGATGACAACCGCCTGGCGCTGATCAACAACCAGCTTGGCAC
GATCATCGCGATGACACATATCCTGCTGCCGTTCATGATCCTGCCGCTGTTCTCGGTCATGAAGACTATCCCGCCAAGCT
ATGTGCGGGCGGCGAAATCGCTGGGGGCCACGAACTTCACCGCCTTCCGGCGCGTGTATTTCCCGCAGAGCATTCCGGGG
ATCGGGGCGGGGTGTATCCTCGTCTTCATCCTGGCCATCGGCTACTATATTACGCCGGAACTTGTGGGCGGACGGACCGG
CACGTTCATCTCCAACCGGATCGCCTACCACATCTCGTCCAGCCTGAACTGGGGTCTGGCGGCGGCGCTTGGCTCGCTCC
TTCTGGCGCTCGTGCTGGTTCTCTACTGGGTCTACGACCGGATCGTGGGCATCGACAACGTGAAGCTGGGATAG

Upstream 100 bases:

>100_bases
GCCGCACTGGCCGCCTACAATGACCCCGACTATCAAGAGGTTGCCGAGATCCGCCGCCGCACCGCCGATAGCGTGATCCT
GGTTGTGGAGGGCGTCTGAG

Downstream 100 bases:

>100_bases
GGGATCACCATGGCTGCGAAGATTGAAACCAAGGAAAACGGCCCCCTGATCGTCAGCGGTGCCCCGGACCTGCGCGATGA
TGCAGGCGGGGCGATTGAAG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSDATGPDQFTAPTPDNALRNAERTDTGPVLAADGTPLKRSLSKALRAQKLRALMLIAPLLIFIMVAFIAPIVDMLFRSV
ENQITSETLPRTVVALENWDPEGLPDDDTYAALTYDFVEAVERRIHTRLGSRLNYETTGMSSLFRRSGRGADDIGEDYAD
QFHDLNPAWEEAATWASLMGAPDWLEDASAADGAGLPFRLSVAAMETLPETADAYRSFARTVQQEDNDNPTAEEPWNSVY
LALYRDLSRVDVSGYDGPQADLLAEAAAAVDGFEGLSPREAWLEVNEDWGDVDVWATIEAFSDPYTAGYFLTSIDLQLTP
QGIESQPEEQQIYVLLFQRTIFMSGMITLMCVLLGYPIAYLLSNLPSKSSNLLLILVLLPFWTSLLVRTSAWKVLLQQQG
VINDILVWVGIVADDNRLALINNQLGTIIAMTHILLPFMILPLFSVMKTIPPSYVRAAKSLGATNFTAFRRVYFPQSIPG
IGAGCILVFILAIGYYITPELVGGRTGTFISNRIAYHISSSLNWGLAAALGSLLLALVLVLYWVYDRIVGIDNVKLG

Sequences:

>Translated_557_residues
MSDATGPDQFTAPTPDNALRNAERTDTGPVLAADGTPLKRSLSKALRAQKLRALMLIAPLLIFIMVAFIAPIVDMLFRSV
ENQITSETLPRTVVALENWDPEGLPDDDTYAALTYDFVEAVERRIHTRLGSRLNYETTGMSSLFRRSGRGADDIGEDYAD
QFHDLNPAWEEAATWASLMGAPDWLEDASAADGAGLPFRLSVAAMETLPETADAYRSFARTVQQEDNDNPTAEEPWNSVY
LALYRDLSRVDVSGYDGPQADLLAEAAAAVDGFEGLSPREAWLEVNEDWGDVDVWATIEAFSDPYTAGYFLTSIDLQLTP
QGIESQPEEQQIYVLLFQRTIFMSGMITLMCVLLGYPIAYLLSNLPSKSSNLLLILVLLPFWTSLLVRTSAWKVLLQQQG
VINDILVWVGIVADDNRLALINNQLGTIIAMTHILLPFMILPLFSVMKTIPPSYVRAAKSLGATNFTAFRRVYFPQSIPG
IGAGCILVFILAIGYYITPELVGGRTGTFISNRIAYHISSSLNWGLAAALGSLLLALVLVLYWVYDRIVGIDNVKLG
>Mature_556_residues
SDATGPDQFTAPTPDNALRNAERTDTGPVLAADGTPLKRSLSKALRAQKLRALMLIAPLLIFIMVAFIAPIVDMLFRSVE
NQITSETLPRTVVALENWDPEGLPDDDTYAALTYDFVEAVERRIHTRLGSRLNYETTGMSSLFRRSGRGADDIGEDYADQ
FHDLNPAWEEAATWASLMGAPDWLEDASAADGAGLPFRLSVAAMETLPETADAYRSFARTVQQEDNDNPTAEEPWNSVYL
ALYRDLSRVDVSGYDGPQADLLAEAAAAVDGFEGLSPREAWLEVNEDWGDVDVWATIEAFSDPYTAGYFLTSIDLQLTPQ
GIESQPEEQQIYVLLFQRTIFMSGMITLMCVLLGYPIAYLLSNLPSKSSNLLLILVLLPFWTSLLVRTSAWKVLLQQQGV
INDILVWVGIVADDNRLALINNQLGTIIAMTHILLPFMILPLFSVMKTIPPSYVRAAKSLGATNFTAFRRVYFPQSIPGI
GAGCILVFILAIGYYITPELVGGRTGTFISNRIAYHISSSLNWGLAAALGSLLLALVLVLYWVYDRIVGIDNVKLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=210, Percent_Identity=35.2380952380952, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1787080, Length=223, Percent_Identity=32.7354260089686, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=31.7829457364341, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 61308; Mature: 61177

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATGPDQFTAPTPDNALRNAERTDTGPVLAADGTPLKRSLSKALRAQKLRALMLIAPL
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIFIMVAFIAPIVDMLFRSVENQITSETLPRTVVALENWDPEGLPDDDTYAALTYDFVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
VERRIHTRLGSRLNYETTGMSSLFRRSGRGADDIGEDYADQFHDLNPAWEEAATWASLMG
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
APDWLEDASAADGAGLPFRLSVAAMETLPETADAYRSFARTVQQEDNDNPTAEEPWNSVY
CCHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LALYRDLSRVDVSGYDGPQADLLAEAAAAVDGFEGLSPREAWLEVNEDWGDVDVWATIEA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCEEHEEEHHH
FSDPYTAGYFLTSIDLQLTPQGIESQPEEQQIYVLLFQRTIFMSGMITLMCVLLGYPIAY
HCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSNLPSKSSNLLLILVLLPFWTSLLVRTSAWKVLLQQQGVINDILVWVGIVADDNRLAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEE
INNQLGTIIAMTHILLPFMILPLFSVMKTIPPSYVRAAKSLGATNFTAFRRVYFPQSIPG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
IGAGCILVFILAIGYYITPELVGGRTGTFISNRIAYHISSSLNWGLAAALGSLLLALVLV
CCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
LYWVYDRIVGIDNVKLG
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDATGPDQFTAPTPDNALRNAERTDTGPVLAADGTPLKRSLSKALRAQKLRALMLIAPL
CCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIFIMVAFIAPIVDMLFRSVENQITSETLPRTVVALENWDPEGLPDDDTYAALTYDFVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
VERRIHTRLGSRLNYETTGMSSLFRRSGRGADDIGEDYADQFHDLNPAWEEAATWASLMG
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
APDWLEDASAADGAGLPFRLSVAAMETLPETADAYRSFARTVQQEDNDNPTAEEPWNSVY
CCHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LALYRDLSRVDVSGYDGPQADLLAEAAAAVDGFEGLSPREAWLEVNEDWGDVDVWATIEA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCEEHEEEHHH
FSDPYTAGYFLTSIDLQLTPQGIESQPEEQQIYVLLFQRTIFMSGMITLMCVLLGYPIAY
HCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSNLPSKSSNLLLILVLLPFWTSLLVRTSAWKVLLQQQGVINDILVWVGIVADDNRLAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCEEEE
INNQLGTIIAMTHILLPFMILPLFSVMKTIPPSYVRAAKSLGATNFTAFRRVYFPQSIPG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
IGAGCILVFILAIGYYITPELVGGRTGTFISNRIAYHISSSLNWGLAAALGSLLLALVLV
CCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHH
LYWVYDRIVGIDNVKLG
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]