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The map label for this gene is puuA [H]

Identifier: 89053618

GI number: 89053618

Start: 1087965

End: 1089404

Strand: Reverse

Name: puuA [H]

Synonym: Jann_1127

Alternate gene names: 89053618

Gene position: 1089404-1087965 (Counterclockwise)

Preceding gene: 89053619

Following gene: 89053617

Centisome position: 25.23

GC content: 65.76

Gene sequence:

>1440_bases
ATGACACCAACGCGGCAGGCGGGCGGCAAACCCCGCTTGCCGTACATGGCCCGCAGGCGTAAGCAAAACCGCATCCCAAC
GCCCGTGTGCCCCATGTCCCAAGATCCCACCGCCTTCCTGCACGACAACCCGCAGATCAAATCCATCCGCATGGTCGCCG
CCGACCTCAACGGACAAGCCCGGGGTAAACGGGTGGCGCGGCGCTTTGCGGGCAAGGCGATGACGGACGGCACGCGGTTC
CCCTATTCGGTCCTGAACCTCGACATCTGGGGGGAGGATATCGACGACAGCCCCCTGGTGTTTGAGGCCGGGGACCCCGA
TGGCATCCTCTACCCCACCGCGCGCGGGCCGGTGCCGGTTCCCTGGCTCGACACGCCCACCGCGATCGTGCCGATCTGGA
TGTTCCACGACGACGGCAGCGCGTATATGGGTGACCCACGCCACGCGCTGGCCCGGGTGATTGACCGCTACACCGCGCGG
GGCCTGACGCCCGTGGTCGCGACCGAGCTGGAGTTCTACATCATCGACGACCGGGGCCGCGAATTACGCGTGCCCCCTTC
GCCGCGCTCGGGCATCCGCCGCCAACAGGCGGAGATTCTGGCGCTGCGCCAATTGGATGCCTTCGATACCTTCCTCACCG
CGCTTTACGACGGGTGCGAGGCGATGGATATCCCCGCCGATACGATGATCTCGGAGGCGGGACCGGGTCAGTTTGAGATC
AACATGGATCACCAGGCAGACGCGATGAAGGCCGCCGATGATGCGTGGCTGTTCAAACTGCTGACCCGAGGCCTTGCCCG
GTCCTACGGATTTGCCGCCAGCTTCATGGCCAAACCTTATCCCGACTACGCAGGCAACGGGTTGCACACCCACTTTTCGG
TGCTGGATGCGGACGGCCACAACATCTTTGACAACGGAACCGATGATGGCTCGGACGTCCTGCACCATGCCGTCGCGGGT
TGCCTAAAGGCAATGCCCGGCTCCATGCTGATCTTCGCGCCCCACGCCTCCAGCTATGACCGCTTCGTGGACGGTGCCCA
CGCGCCCACCGCCGCCGCCTGGGCCTATGAAAACCGCACCACCGCGCTCCGCATCCCCGGCGGCGCCCCCGCTGCGCGCC
GGATCGAACATCGGGTCGCGGGTGGGGATATCAATCCGTACCTCTTCCTAGCCGCCGTCCTGGGCGCAGCGCTCGACGGC
ATTGAGCAGGGCACGACCCCGCCCCCCGCCATCACCGGCAGCGCCTATGGCGCGGACCTGCCGCAACTGCCCCAGACCTG
GAGCGACGCACTCGCAGCCTTCGACGAAAGCCCTGAAATCAAACGCATCTTCGCGTCCGAGCTGCGGGAGACCTTTCTCG
CCACCAAACGCCAAGAGCTCCGCTACATGGCCGACCTTTCGCCGGAGGAGCAGCAGGAACTCTACCTCGACACCGTCTGA

Upstream 100 bases:

>100_bases
TCGGAGGTATCCGTTGCCCCCGTGCCCTTGCCCGCTGGCGTCTGGCTCCTCTTGGTGAGCCTCGGCGGTCTGGCGGGCCT
GCGCCGTCTGCGCGCCTGAC

Downstream 100 bases:

>100_bases
CATACCGCACGAAAGCCTTTTCGAAAAGGCTTGCCAGAGCCTTGCAAGGCTCTGTGCCACGTCCTTGCAAGGACGTGTGC
AAACTCTTCGAAGAGTTTGC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTPTRQAGGKPRLPYMARRRKQNRIPTPVCPMSQDPTAFLHDNPQIKSIRMVAADLNGQARGKRVARRFAGKAMTDGTRF
PYSVLNLDIWGEDIDDSPLVFEAGDPDGILYPTARGPVPVPWLDTPTAIVPIWMFHDDGSAYMGDPRHALARVIDRYTAR
GLTPVVATELEFYIIDDRGRELRVPPSPRSGIRRQQAEILALRQLDAFDTFLTALYDGCEAMDIPADTMISEAGPGQFEI
NMDHQADAMKAADDAWLFKLLTRGLARSYGFAASFMAKPYPDYAGNGLHTHFSVLDADGHNIFDNGTDDGSDVLHHAVAG
CLKAMPGSMLIFAPHASSYDRFVDGAHAPTAAAWAYENRTTALRIPGGAPAARRIEHRVAGGDINPYLFLAAVLGAALDG
IEQGTTPPPAITGSAYGADLPQLPQTWSDALAAFDESPEIKRIFASELRETFLATKRQELRYMADLSPEEQQELYLDTV

Sequences:

>Translated_479_residues
MTPTRQAGGKPRLPYMARRRKQNRIPTPVCPMSQDPTAFLHDNPQIKSIRMVAADLNGQARGKRVARRFAGKAMTDGTRF
PYSVLNLDIWGEDIDDSPLVFEAGDPDGILYPTARGPVPVPWLDTPTAIVPIWMFHDDGSAYMGDPRHALARVIDRYTAR
GLTPVVATELEFYIIDDRGRELRVPPSPRSGIRRQQAEILALRQLDAFDTFLTALYDGCEAMDIPADTMISEAGPGQFEI
NMDHQADAMKAADDAWLFKLLTRGLARSYGFAASFMAKPYPDYAGNGLHTHFSVLDADGHNIFDNGTDDGSDVLHHAVAG
CLKAMPGSMLIFAPHASSYDRFVDGAHAPTAAAWAYENRTTALRIPGGAPAARRIEHRVAGGDINPYLFLAAVLGAALDG
IEQGTTPPPAITGSAYGADLPQLPQTWSDALAAFDESPEIKRIFASELRETFLATKRQELRYMADLSPEEQQELYLDTV
>Mature_478_residues
TPTRQAGGKPRLPYMARRRKQNRIPTPVCPMSQDPTAFLHDNPQIKSIRMVAADLNGQARGKRVARRFAGKAMTDGTRFP
YSVLNLDIWGEDIDDSPLVFEAGDPDGILYPTARGPVPVPWLDTPTAIVPIWMFHDDGSAYMGDPRHALARVIDRYTARG
LTPVVATELEFYIIDDRGRELRVPPSPRSGIRRQQAEILALRQLDAFDTFLTALYDGCEAMDIPADTMISEAGPGQFEIN
MDHQADAMKAADDAWLFKLLTRGLARSYGFAASFMAKPYPDYAGNGLHTHFSVLDADGHNIFDNGTDDGSDVLHHAVAGC
LKAMPGSMLIFAPHASSYDRFVDGAHAPTAAAWAYENRTTALRIPGGAPAARRIEHRVAGGDINPYLFLAAVLGAALDGI
EQGTTPPPAITGSAYGADLPQLPQTWSDALAAFDESPEIKRIFASELRETFLATKRQELRYMADLSPEEQQELYLDTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=427, Percent_Identity=25.0585480093677, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081870, Length=457, Percent_Identity=35.0109409190372, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1790301, Length=221, Percent_Identity=32.1266968325792, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52512; Mature: 52381

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTRQAGGKPRLPYMARRRKQNRIPTPVCPMSQDPTAFLHDNPQIKSIRMVAADLNGQA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCH
RGKRVARRFAGKAMTDGTRFPYSVLNLDIWGEDIDDSPLVFEAGDPDGILYPTARGPVPV
HHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCCC
PWLDTPTAIVPIWMFHDDGSAYMGDPRHALARVIDRYTARGLTPVVATELEFYIIDDRGR
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCC
ELRVPPSPRSGIRRQQAEILALRQLDAFDTFLTALYDGCEAMDIPADTMISEAGPGQFEI
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEE
NMDHQADAMKAADDAWLFKLLTRGLARSYGFAASFMAKPYPDYAGNGLHTHFSVLDADGH
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
NIFDNGTDDGSDVLHHAVAGCLKAMPGSMLIFAPHASSYDRFVDGAHAPTAAAWAYENRT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCCHHHEEECCCC
TALRIPGGAPAARRIEHRVAGGDINPYLFLAAVLGAALDGIEQGTTPPPAITGSAYGADL
EEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PQLPQTWSDALAAFDESPEIKRIFASELRETFLATKRQELRYMADLSPEEQQELYLDTV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCC
>Mature Secondary Structure 
TPTRQAGGKPRLPYMARRRKQNRIPTPVCPMSQDPTAFLHDNPQIKSIRMVAADLNGQA
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCH
RGKRVARRFAGKAMTDGTRFPYSVLNLDIWGEDIDDSPLVFEAGDPDGILYPTARGPVPV
HHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCCC
PWLDTPTAIVPIWMFHDDGSAYMGDPRHALARVIDRYTARGLTPVVATELEFYIIDDRGR
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCC
ELRVPPSPRSGIRRQQAEILALRQLDAFDTFLTALYDGCEAMDIPADTMISEAGPGQFEI
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEE
NMDHQADAMKAADDAWLFKLLTRGLARSYGFAASFMAKPYPDYAGNGLHTHFSVLDADGH
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
NIFDNGTDDGSDVLHHAVAGCLKAMPGSMLIFAPHASSYDRFVDGAHAPTAAAWAYENRT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCCHHHEEECCCC
TALRIPGGAPAARRIEHRVAGGDINPYLFLAAVLGAALDGIEQGTTPPPAITGSAYGADL
EEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PQLPQTWSDALAAFDESPEIKRIFASELRETFLATKRQELRYMADLSPEEQQELYLDTV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]