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The map label for this gene is pcm

Identifier: 89053558

GI number: 89053558

Start: 1028831

End: 1029499

Strand: Reverse

Name: pcm

Synonym: Jann_1067

Alternate gene names: 89053558

Gene position: 1029499-1028831 (Counterclockwise)

Preceding gene: 89053559

Following gene: 89053556

Centisome position: 23.84

GC content: 65.17

Gene sequence:

>669_bases
ATGAGCGACCTCGGGCGCGGCGAGGGGACAGGCTTTGATGCCGAGCGCAAGATGCAGTTCCTCTACCAGCTGCGCCAGAA
GGGCGTCATGGATAAACGCGTGCTGACCGCGATGGAGCATGTGGATCGCGGCGCGTTCGTGCGCGGTCATTTCGCGTCCC
GCGCCTATGAAGACGTGCCCCTGCCGATCAGTTCGGGCCAGACGATCAGCCAGCCCTCCGTCGTGGGGCTGATGACGCAG
GCGCTGAACGTCCAGCCGCGCGATACGGTGCTGGAGGTGGGCACAGGGTCCGGCTATCAGGCCGCGATCCTCAGCCATCT
GGCGCGGCGCATCTACACGATTGACCGCCACCGCAATCTGACCCGCGAGGCGGAGATCATTTTCACGCGGATGGGCTTGG
TCAATATCACGGTGCTGACCCGCGACGGCTCCTTCGGGCTGCCGGATCAGGGCCCCTTTGACCGGATCCTTATCACCGCC
GCCGCTGAAGACCCCCCGGGTCCGCTGCTGCAACAACTCAAGGTCGGGGGCGTGATGGTCGTGCCGGTCGGCCAATCGGA
CACGGTGCAAAGCCTGATCAAGGTCACGCGGCTGGAGACGGGCTTTGACTATGACGAGTTGATGCCCGTCCGCTTTGTGC
CGCTGGTGGAGGGCACGGCCCGCGACTAG

Upstream 100 bases:

>100_bases
GATCTGACGGCCTATGATCAGCTTCAATCCCTGAAAGACGCCATCGAATGAAAGAGGCGGGGCGAATGAGAGAGGCGGGG
CGAATGAGGGGGGCGGGCGC

Downstream 100 bases:

>100_bases
GCCGCGCAGCGTCCCGAACCGCCGTGCTTGCCGCTGATCTCAGCGCGCGACCAACCGGCCCATGCCCCATGGCCCCGTGC
TACCCCGCCAACGCAAATTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSDLGRGEGTGFDAERKMQFLYQLRQKGVMDKRVLTAMEHVDRGAFVRGHFASRAYEDVPLPISSGQTISQPSVVGLMTQ
ALNVQPRDTVLEVGTGSGYQAAILSHLARRIYTIDRHRNLTREAEIIFTRMGLVNITVLTRDGSFGLPDQGPFDRILITA
AAEDPPGPLLQQLKVGGVMVVPVGQSDTVQSLIKVTRLETGFDYDELMPVRFVPLVEGTARD

Sequences:

>Translated_222_residues
MSDLGRGEGTGFDAERKMQFLYQLRQKGVMDKRVLTAMEHVDRGAFVRGHFASRAYEDVPLPISSGQTISQPSVVGLMTQ
ALNVQPRDTVLEVGTGSGYQAAILSHLARRIYTIDRHRNLTREAEIIFTRMGLVNITVLTRDGSFGLPDQGPFDRILITA
AAEDPPGPLLQQLKVGGVMVVPVGQSDTVQSLIKVTRLETGFDYDELMPVRFVPLVEGTARD
>Mature_221_residues
SDLGRGEGTGFDAERKMQFLYQLRQKGVMDKRVLTAMEHVDRGAFVRGHFASRAYEDVPLPISSGQTISQPSVVGLMTQA
LNVQPRDTVLEVGTGSGYQAAILSHLARRIYTIDRHRNLTREAEIIFTRMGLVNITVLTRDGSFGLPDQGPFDRILITAA
AEDPPGPLLQQLKVGGVMVVPVGQSDTVQSLIKVTRLETGFDYDELMPVRFVPLVEGTARD

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=9e-12,
Organism=Escherichia coli, GI1789100, Length=205, Percent_Identity=41.4634146341463, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=215, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_JANSC (Q28TH8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509009.1
- ProteinModelPortal:   Q28TH8
- SMR:   Q28TH8
- STRING:   Q28TH8
- GeneID:   3933511
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1067
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- PhylomeDB:   Q28TH8
- ProtClustDB:   CLSK933941
- BioCyc:   JSP290400:JANN_1067-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24426; Mature: 24295

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLGRGEGTGFDAERKMQFLYQLRQKGVMDKRVLTAMEHVDRGAFVRGHFASRAYEDVP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECHHHHCCCCCCC
LPISSGQTISQPSVVGLMTQALNVQPRDTVLEVGTGSGYQAAILSHLARRIYTIDRHRNL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
TREAEIIFTRMGLVNITVLTRDGSFGLPDQGPFDRILITAAAEDPPGPLLQQLKVGGVMV
CHHHHHHEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEE
VPVGQSDTVQSLIKVTRLETGFDYDELMPVRFVPLVEGTARD
EECCCCHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SDLGRGEGTGFDAERKMQFLYQLRQKGVMDKRVLTAMEHVDRGAFVRGHFASRAYEDVP
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECHHHHCCCCCCC
LPISSGQTISQPSVVGLMTQALNVQPRDTVLEVGTGSGYQAAILSHLARRIYTIDRHRNL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
TREAEIIFTRMGLVNITVLTRDGSFGLPDQGPFDRILITAAAEDPPGPLLQQLKVGGVMV
CHHHHHHEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEE
VPVGQSDTVQSLIKVTRLETGFDYDELMPVRFVPLVEGTARD
EECCCCHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA