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The map label for this gene is gloB

Identifier: 89053533

GI number: 89053533

Start: 998405

End: 999175

Strand: Reverse

Name: gloB

Synonym: Jann_1042

Alternate gene names: 89053533

Gene position: 999175-998405 (Counterclockwise)

Preceding gene: 89053544

Following gene: 89053532

Centisome position: 23.14

GC content: 64.33

Gene sequence:

>771_bases
ATGGCCGATCCGCAGATCATCACCATTCCCTGCCTTCAGGACAACTACGCCTTCCTGCTGCACGACCCCTCTACCGGGGC
CACCGCCTGCATCGACGTGCCGGACGCCGCCCCGATCCAGGCCGCTTTGGGGGCGCAGGGCTGGACATTGACCGACATTC
TTCTCACCCACCATCATTGGGACCACATCGACGGCGTGCCCGCCCTTGTGGAGGCCACCGGCGCGAAGGTCTGGGGCGCG
GCAGCTGACGCCCATCGCCTGCCCCCGCTTGATCACGCATTGGCGGAGGGCGATACAATCCGCATCGGCGCGCTGGAGGG
CGACGTCATGGATGTCTCCGGCCACACGGTGGGCCATGTGGCCTTCCACTTTCCCGCCGCGACCGCAGTTTTCACGGCAG
ACAGCCTGATGGCTCTGGGGTGTGGGCGCCTCTTTGAAGGCACGCCCGCGCAGATGCATGGCTCCCTTGAGAAGCTGAAA
ATGCTGCCGCCCGGGACGCTTGTGTGTTCCGGCCACGAATATACCGCGTCGAATGCGCGGTTTGCATTAACCATTGAACC
GGACAATCCAGACCTTATCTTTCGGGTCGAGCGTATCACCGCTGCTCGGGCGGCGGGTCAGCCCACCGTTCCCTCCACCC
TGGCCGAGGAATTGGCCACCAATCCGTTCCTGCGCGCAGACGCGCCGACCGTTGCCGCGCCTCTCGATATGGAGGGCGCA
GATCCCGTCGACGTTTTCACCCGCATCCGTGCCCAAAAAGACAGCTTCTGA

Upstream 100 bases:

>100_bases
CGCAGATCTACGACGTCCAGATGCATCCGACTTCCTGTTGCATACATGCGCCAAGGGGCGCAGGCTTGGTCCTAACGTAG
CCATTGAAGGGACAAAGACC

Downstream 100 bases:

>100_bases
GCGTTAATCTTTTAAGTACGCGTTTTCAGGGCATTAGACCCCTGCTCAACCTTGAGCATGCTCAAAGTGCCTCGGAAACA
CACAGTGGTGATGCGAATTT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MADPQIITIPCLQDNYAFLLHDPSTGATACIDVPDAAPIQAALGAQGWTLTDILLTHHHWDHIDGVPALVEATGAKVWGA
AADAHRLPPLDHALAEGDTIRIGALEGDVMDVSGHTVGHVAFHFPAATAVFTADSLMALGCGRLFEGTPAQMHGSLEKLK
MLPPGTLVCSGHEYTASNARFALTIEPDNPDLIFRVERITAARAAGQPTVPSTLAEELATNPFLRADAPTVAAPLDMEGA
DPVDVFTRIRAQKDSF

Sequences:

>Translated_256_residues
MADPQIITIPCLQDNYAFLLHDPSTGATACIDVPDAAPIQAALGAQGWTLTDILLTHHHWDHIDGVPALVEATGAKVWGA
AADAHRLPPLDHALAEGDTIRIGALEGDVMDVSGHTVGHVAFHFPAATAVFTADSLMALGCGRLFEGTPAQMHGSLEKLK
MLPPGTLVCSGHEYTASNARFALTIEPDNPDLIFRVERITAARAAGQPTVPSTLAEELATNPFLRADAPTVAAPLDMEGA
DPVDVFTRIRAQKDSF
>Mature_255_residues
ADPQIITIPCLQDNYAFLLHDPSTGATACIDVPDAAPIQAALGAQGWTLTDILLTHHHWDHIDGVPALVEATGAKVWGAA
ADAHRLPPLDHALAEGDTIRIGALEGDVMDVSGHTVGHVAFHFPAATAVFTADSLMALGCGRLFEGTPAQMHGSLEKLKM
LPPGTLVCSGHEYTASNARFALTIEPDNPDLIFRVERITAARAAGQPTVPSTLAEELATNPFLRADAPTVAAPLDMEGAD
PVDVFTRIRAQKDSF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=37.0656370656371, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=37.0656370656371, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI14150041, Length=255, Percent_Identity=36.4705882352941, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=34.8017621145374, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI21703352, Length=227, Percent_Identity=34.8017621145374, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI46361987, Length=191, Percent_Identity=38.2198952879581, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1786406, Length=252, Percent_Identity=36.5079365079365, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17536925, Length=254, Percent_Identity=32.6771653543307, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320478, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356335, Length=263, Percent_Identity=39.1634980988593, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667711, Length=263, Percent_Identity=39.1634980988593, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667703, Length=262, Percent_Identity=38.5496183206107, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI221330176, Length=189, Percent_Identity=31.2169312169312, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_JANSC (Q28TK3)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508984.1
- ProteinModelPortal:   Q28TK3
- SMR:   Q28TK3
- STRING:   Q28TK3
- GeneID:   3933486
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1042
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   FGHEYTL
- PhylomeDB:   Q28TK3
- ProtClustDB:   CLSK836335
- BioCyc:   JSP290400:JANN_1042-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27033; Mature: 26902

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPQIITIPCLQDNYAFLLHDPSTGATACIDVPDAAPIQAALGAQGWTLTDILLTHHHW
CCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCHHHHEECCCCC
DHIDGVPALVEATGAKVWGAAADAHRLPPLDHALAEGDTIRIGALEGDVMDVSGHTVGHV
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCEEEECCCEEEEE
AFHFPAATAVFTADSLMALGCGRLFEGTPAQMHGSLEKLKMLPPGTLVCSGHEYTASNAR
EEECCCHHEEHHHHHHHHHCCCHHCCCCCHHHHCCHHHEECCCCCEEEECCCEEECCCCE
FALTIEPDNPDLIFRVERITAARAAGQPTVPSTLAEELATNPFLRADAPTVAAPLDMEGA
EEEEECCCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCEECCCCCCCC
DPVDVFTRIRAQKDSF
CHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADPQIITIPCLQDNYAFLLHDPSTGATACIDVPDAAPIQAALGAQGWTLTDILLTHHHW
CCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCHHHHEECCCCC
DHIDGVPALVEATGAKVWGAAADAHRLPPLDHALAEGDTIRIGALEGDVMDVSGHTVGHV
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCEEEECCCEEEEE
AFHFPAATAVFTADSLMALGCGRLFEGTPAQMHGSLEKLKMLPPGTLVCSGHEYTASNAR
EEECCCHHEEHHHHHHHHHCCCHHCCCCCHHHHCCHHHEECCCCCEEEECCCEEECCCCE
FALTIEPDNPDLIFRVERITAARAAGQPTVPSTLAEELATNPFLRADAPTVAAPLDMEGA
EEEEECCCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCEECCCCCCCC
DPVDVFTRIRAQKDSF
CHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA