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The map label for this gene is rimO

Identifier: 89053506

GI number: 89053506

Start: 968162

End: 969538

Strand: Reverse

Name: rimO

Synonym: Jann_1015

Alternate gene names: 89053506

Gene position: 969538-968162 (Counterclockwise)

Preceding gene: 89053509

Following gene: 89053502

Centisome position: 22.45

GC content: 62.75

Gene sequence:

>1377_bases
ATGAGCCAGAACCCACCCCTCCTCCGCCCCGATCTCGCACGGGCCCGCGTGCCTGATGACGCCCGCCCCGGCCAGCCCCG
CATCGGCATGGTGTCACTTGGCTGCCCGAAAGCGCTGGTCGATAGCGAACGCATCCTCACCCGCCTGCGCGCGGAGGGCT
ATGCGATCAGCCCCGATTATACCGGCGCGGAGGCGGTGATCGTGAACACCTGCGGCTTTCTCGACAGTGCCAAGCTGGAA
AGTCTGGAGGCGATTGGCGAGGCGTTGGAGGCGAATGGCAAGGTCATCGTGACAGGCTGTCTGGGTGCGGAGCCTGAGTA
TATCACCGGCGCGCATCCCACGGTTCTGGCCGTCACCGGACCGCAGCAATATGAGCAAGTGCTTGATGCTGTCCATGGGG
CCGTGCCCCCCGCGCCCGACCCGTTCATCGACCTTTTGCCACCCGCAGGCGTCAAGCTGACACCCCGCCATTACGCCTAT
CTCAAGATCGCCGAGGGCTGCGATCACAAGTGCAAGTTCTGCATCATCCCCGACATGCGCGGCAAGCTTGTGTCCCGCCC
CCAGACCGCCGTGATGCGAGAGGCCGAGAAGTTGGTGGACAGTGGCGTTAAGGAATTGCTGATCATCTCCCAGGACACGT
CTGCTTACGGTGTGGACTGGAAGGACCGGAACAAAGCCGGTGATGAGAAGTTTCCTATCCTCAATCTATCCCGCGACTTA
TCCACTCTGGGTGCCTGGGTTCGCTTACACTACGTCTACCCCTATCCCCATGTGCGCGAATTGATCCCCTTGATGGCGGA
TCCCGCGAACGGCCTGCTGCCCTACCTCGACATTCCGTTTCAGCATGCCCATCCCGATGTCCTCAAGCGCATGGCACGGC
CCGCCGCTGCGTCCCGGACTCTGGATGAGATCGCCGCCTGGCGACGCGACTGCCCGAACATCACCCTGCGCTCCACCTTC
ATAGTCGGCTACCCCGGCGAGACGGAGGCGGAGTTCCAAACCCTGCTCGATTGGCTGGACGAGGCGCAGCTCGACCGCAT
CGGATGTTTTCAATACGAAAACGTCGACGGCGCGCGCTCCAACGCCCTGCCGGATCATGTTGCGCCGGAGGTCAAGCAGG
ACCGCTGGGACCGGTTCATGGAAAAGGCACAAGCCATTTCAGAGGCGAAGCTGCAAGCCAAGGTGGGCCGCACGATGCAG
GTCCTTGTGGACAGCGTGGACGAGGAGGGCGCCACCTGTCGCACCGTGGCAGACGCACCTGAGATCGACGGCAACCTCTT
CATCGACGAGGGGTTTGACGGCCTGACACCCGGTGACTTGGTGACGGTCGAGGTTGACGAGGCCAGCGAGTACGACCTCT
GGGGCAAGCTGACTTAG

Upstream 100 bases:

>100_bases
GGCGCCCGCCGCCGCAAATCCAAAATCTTGCGATGGCGGCAAGGGTTTGCTTATCCAACGCCCCTTTCCACAGGTCAGCC
CAGCCGCTATCTGGCAGGGC

Downstream 100 bases:

>100_bases
GCACCCATGGGCACCCTCAGGCGGCATCGTTATTCCGCGTTGAGTAGGGGCATGTGTAGTTACAGAAAAACTACAGGATA
ACTACAGGTTCACTACACCG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSQNPPLLRPDLARARVPDDARPGQPRIGMVSLGCPKALVDSERILTRLRAEGYAISPDYTGAEAVIVNTCGFLDSAKLE
SLEAIGEALEANGKVIVTGCLGAEPEYITGAHPTVLAVTGPQQYEQVLDAVHGAVPPAPDPFIDLLPPAGVKLTPRHYAY
LKIAEGCDHKCKFCIIPDMRGKLVSRPQTAVMREAEKLVDSGVKELLIISQDTSAYGVDWKDRNKAGDEKFPILNLSRDL
STLGAWVRLHYVYPYPHVRELIPLMADPANGLLPYLDIPFQHAHPDVLKRMARPAAASRTLDEIAAWRRDCPNITLRSTF
IVGYPGETEAEFQTLLDWLDEAQLDRIGCFQYENVDGARSNALPDHVAPEVKQDRWDRFMEKAQAISEAKLQAKVGRTMQ
VLVDSVDEEGATCRTVADAPEIDGNLFIDEGFDGLTPGDLVTVEVDEASEYDLWGKLT

Sequences:

>Translated_458_residues
MSQNPPLLRPDLARARVPDDARPGQPRIGMVSLGCPKALVDSERILTRLRAEGYAISPDYTGAEAVIVNTCGFLDSAKLE
SLEAIGEALEANGKVIVTGCLGAEPEYITGAHPTVLAVTGPQQYEQVLDAVHGAVPPAPDPFIDLLPPAGVKLTPRHYAY
LKIAEGCDHKCKFCIIPDMRGKLVSRPQTAVMREAEKLVDSGVKELLIISQDTSAYGVDWKDRNKAGDEKFPILNLSRDL
STLGAWVRLHYVYPYPHVRELIPLMADPANGLLPYLDIPFQHAHPDVLKRMARPAAASRTLDEIAAWRRDCPNITLRSTF
IVGYPGETEAEFQTLLDWLDEAQLDRIGCFQYENVDGARSNALPDHVAPEVKQDRWDRFMEKAQAISEAKLQAKVGRTMQ
VLVDSVDEEGATCRTVADAPEIDGNLFIDEGFDGLTPGDLVTVEVDEASEYDLWGKLT
>Mature_457_residues
SQNPPLLRPDLARARVPDDARPGQPRIGMVSLGCPKALVDSERILTRLRAEGYAISPDYTGAEAVIVNTCGFLDSAKLES
LEAIGEALEANGKVIVTGCLGAEPEYITGAHPTVLAVTGPQQYEQVLDAVHGAVPPAPDPFIDLLPPAGVKLTPRHYAYL
KIAEGCDHKCKFCIIPDMRGKLVSRPQTAVMREAEKLVDSGVKELLIISQDTSAYGVDWKDRNKAGDEKFPILNLSRDLS
TLGAWVRLHYVYPYPHVRELIPLMADPANGLLPYLDIPFQHAHPDVLKRMARPAAASRTLDEIAAWRRDCPNITLRSTFI
VGYPGETEAEFQTLLDWLDEAQLDRIGCFQYENVDGARSNALPDHVAPEVKQDRWDRFMEKAQAISEAKLQAKVGRTMQV
LVDSVDEEGATCRTVADAPEIDGNLFIDEGFDGLTPGDLVTVEVDEASEYDLWGKLT

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=24.5360824742268, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=24.5360824742268, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=24.507658643326, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=62.0689655172414, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=25.2771618625277, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17553146, Length=483, Percent_Identity=23.8095238095238, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356207, Length=390, Percent_Identity=24.3589743589744, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_JANSC (Q28TN0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508957.1
- ProteinModelPortal:   Q28TN0
- SMR:   Q28TN0
- STRING:   Q28TN0
- GeneID:   3933459
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1015
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q28TN0
- ProtClustDB:   PRK14862
- BioCyc:   JSP290400:JANN_1015-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50348; Mature: 50217

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNPPLLRPDLARARVPDDARPGQPRIGMVSLGCPKALVDSERILTRLRAEGYAISPDY
CCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCC
TGAEAVIVNTCGFLDSAKLESLEAIGEALEANGKVIVTGCLGAEPEYITGAHPTVLAVTG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCEEEEEEC
PQQYEQVLDAVHGAVPPAPDPFIDLLPPAGVKLTPRHYAYLKIAEGCDHKCKFCIIPDMR
HHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEECCCCEEEEEECCCCCCCCEEEECCCCC
GKLVSRPQTAVMREAEKLVDSGVKELLIISQDTSAYGVDWKDRNKAGDEKFPILNLSRDL
CHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECHHHH
STLGAWVRLHYVYPYPHVRELIPLMADPANGLLPYLDIPFQHAHPDVLKRMARPAAASRT
HHHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCHHHHHH
LDEIAAWRRDCPNITLRSTFIVGYPGETEAEFQTLLDWLDEAQLDRIGCFQYENVDGARS
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCC
NALPDHVAPEVKQDRWDRFMEKAQAISEAKLQAKVGRTMQVLVDSVDEEGATCRTVADAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCC
EIDGNLFIDEGFDGLTPGDLVTVEVDEASEYDLWGKLT
CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SQNPPLLRPDLARARVPDDARPGQPRIGMVSLGCPKALVDSERILTRLRAEGYAISPDY
CCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCC
TGAEAVIVNTCGFLDSAKLESLEAIGEALEANGKVIVTGCLGAEPEYITGAHPTVLAVTG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCEEEEEEC
PQQYEQVLDAVHGAVPPAPDPFIDLLPPAGVKLTPRHYAYLKIAEGCDHKCKFCIIPDMR
HHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEECCCCEEEEEECCCCCCCCEEEECCCCC
GKLVSRPQTAVMREAEKLVDSGVKELLIISQDTSAYGVDWKDRNKAGDEKFPILNLSRDL
CHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECHHHH
STLGAWVRLHYVYPYPHVRELIPLMADPANGLLPYLDIPFQHAHPDVLKRMARPAAASRT
HHHHHEEEEEEECCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCHHHHHH
LDEIAAWRRDCPNITLRSTFIVGYPGETEAEFQTLLDWLDEAQLDRIGCFQYENVDGARS
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCC
NALPDHVAPEVKQDRWDRFMEKAQAISEAKLQAKVGRTMQVLVDSVDEEGATCRTVADAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCC
EIDGNLFIDEGFDGLTPGDLVTVEVDEASEYDLWGKLT
CCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA