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The map label for this gene is gspE [C]

Identifier: 89053485

GI number: 89053485

Start: 951882

End: 953372

Strand: Reverse

Name: gspE [C]

Synonym: Jann_0994

Alternate gene names: 89053485

Gene position: 953372-951882 (Counterclockwise)

Preceding gene: 89053486

Following gene: 89053484

Centisome position: 22.08

GC content: 60.9

Gene sequence:

>1491_bases
ATGTTTTCCAAATACAAAAAGGCTCCAAAGGGTGGCGAGGTCGCGCATATTACCGACGTGGCAGCCGCGCCAAAGACCGA
CGACGCGCCCGCACCAGAGAATTCATCAGCCAAAGCGCAGACCCCCGCGCCGAAAGTCGCGCGCAAACCGGGGGGATCTG
CCGCCGCCAAGCCGGCCACGCCCGAAGCCGCGGGGGCTGAGGACAAGGACCGCAAGCGCCGTCTGCGCCTGGATGAAATC
AAGACAGAGATGCATCACCGGCTGCTCGACAACCTCAACCTCTCCGCGCTGGAGACGGCCAAGGAATCGGAACTTCGGTC
TGAAATCACGTCGATCACGTCTGAGCAGCTTGGGGAGATGGGCGTCGTCCTGAACCGGGAGGATCGCCAGACCCTCAACA
TTGAGCTTTTCGATGAAGTCACCGGTCTTGGTCCGTTGGAGCCTTTGCTGAAAGACGACGGCGTCAACGATATTCTCGTC
AACGGTCCCAACCGGGTGTTCATTGAACGTGCCGGCAAGCTTCAACTCTCGGACGTGCGCTTCAAGGACGAGCGTCACCT
ACTTCGGATCATCGACAAGATCGTGTCCGCCGTCGGCCGGCGGGTCGACGAAAGCAATCCTTACGTGGACGCACGCCTGA
AAGACGGCTCACGTTTCAACGCGATGGTGCCACCGGTGGCCGTCGATGGCTCGCTCGTCTCCATTCGTAAGTTCAAGAAA
GAAAAGCTGGGGGTCGACGATCTGGTTAAGTTCGGCGCCTTTTCCGAGGAGATGGCCGCCTACATTCAGGCTGCCGTGGC
CTGTCGCCTGAACGTCATCGTCTCTGGCGGTACGGGCTCGGGCAAAACCACAACGCTTAACGCGCTATCGTCGTTCATCG
ACAATTCCGAACGGATCCTGACGATTGAGGATACGGCGGAACTTCAACTCCAGCAGGTTCACGTGGGCCGGATGGAAAGC
CGCCCACCGAACGTGGAGGGCAAAGGCGCCGTGACCCAGCGGGACTGTCTCCGCAACGCCCTGCGGATGCGCCCCGACCG
TATCATTGTGGGTGAGACACGCGGCGAAGAGGTCATCGATATGCTCCAGGCCATGAATACCGGCCACGACGGATCCATGA
CGACGATCCACGCCAACAACGCCCGCGACGCCTGCTCACGCCTTGAAAACATGGTCGCTATGGCAGGGATCGAGATGCCG
CTGAAGGCTGTTCGCGCCCAGATCTCCAGCGCTGTGCACCTGATCGTGCAGGCCTCTCGTCTGCAGGACGGCTCGCGTCG
CATGGTCTCCATCACCGAAGTGACGGGGATGGAGGGCGAAGTGATCTCCATGCAGGAGGTGTTCCGCTTCCAGCGCACCG
GGCTCCAGCCCGACGGCAAGATCCTCGGCCATTTCACCGCCTGCGGCGTGCGGTCCTATTATTCCGAGCGGTTCCGGCAG
TGGGGCTATGACCTGCCCGCGTCGATCTATGAACCCATGGCAGCCCAGTAA

Upstream 100 bases:

>100_bases
GAAGCTGCCAAGAAGAACCCGCTTCGGAAGGAAATCCTGAAGCTGTCCGAGGGCCTGTTCAAGGCCATGGCAGCGGATGC
GCGAGCCAAAGGGTAAGATT

Downstream 100 bases:

>100_bases
GGAACCCGTCCGATGGACCTCTCAATCGAACCTCTCATCTATATCGGCATTTTTGTTGGTGTGATCATGCTGGTGAACGG
CGTCTATCTGGCGGTGTTTG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MFSKYKKAPKGGEVAHITDVAAAPKTDDAPAPENSSAKAQTPAPKVARKPGGSAAAKPATPEAAGAEDKDRKRRLRLDEI
KTEMHHRLLDNLNLSALETAKESELRSEITSITSEQLGEMGVVLNREDRQTLNIELFDEVTGLGPLEPLLKDDGVNDILV
NGPNRVFIERAGKLQLSDVRFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKK
EKLGVDDLVKFGAFSEEMAAYIQAAVACRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQVHVGRMES
RPPNVEGKGAVTQRDCLRNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANNARDACSRLENMVAMAGIEMP
LKAVRAQISSAVHLIVQASRLQDGSRRMVSITEVTGMEGEVISMQEVFRFQRTGLQPDGKILGHFTACGVRSYYSERFRQ
WGYDLPASIYEPMAAQ

Sequences:

>Translated_496_residues
MFSKYKKAPKGGEVAHITDVAAAPKTDDAPAPENSSAKAQTPAPKVARKPGGSAAAKPATPEAAGAEDKDRKRRLRLDEI
KTEMHHRLLDNLNLSALETAKESELRSEITSITSEQLGEMGVVLNREDRQTLNIELFDEVTGLGPLEPLLKDDGVNDILV
NGPNRVFIERAGKLQLSDVRFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKK
EKLGVDDLVKFGAFSEEMAAYIQAAVACRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQVHVGRMES
RPPNVEGKGAVTQRDCLRNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANNARDACSRLENMVAMAGIEMP
LKAVRAQISSAVHLIVQASRLQDGSRRMVSITEVTGMEGEVISMQEVFRFQRTGLQPDGKILGHFTACGVRSYYSERFRQ
WGYDLPASIYEPMAAQ
>Mature_496_residues
MFSKYKKAPKGGEVAHITDVAAAPKTDDAPAPENSSAKAQTPAPKVARKPGGSAAAKPATPEAAGAEDKDRKRRLRLDEI
KTEMHHRLLDNLNLSALETAKESELRSEITSITSEQLGEMGVVLNREDRQTLNIELFDEVTGLGPLEPLLKDDGVNDILV
NGPNRVFIERAGKLQLSDVRFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKK
EKLGVDDLVKFGAFSEEMAAYIQAAVACRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQVHVGRMES
RPPNVEGKGAVTQRDCLRNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANNARDACSRLENMVAMAGIEMP
LKAVRAQISSAVHLIVQASRLQDGSRRMVSITEVTGMEGEVISMQEVFRFQRTGLQPDGKILGHFTACGVRSYYSERFRQ
WGYDLPASIYEPMAAQ

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=122, Percent_Identity=37.7049180327869, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54388; Mature: 54388

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKYKKAPKGGEVAHITDVAAAPKTDDAPAPENSSAKAQTPAPKVARKPGGSAAAKPAT
CCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
PEAAGAEDKDRKRRLRLDEIKTEMHHRLLDNLNLSALETAKESELRSEITSITSEQLGEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVVLNREDRQTLNIELFDEVTGLGPLEPLLKDDGVNDILVNGPNRVFIERAGKLQLSDVR
CEEEECCCCCEEEEEEHHHHCCCCCCCHHHHCCCCCEEEECCCCEEEEECCCCEEEECCC
FKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKK
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHH
EKLGVDDLVKFGAFSEEMAAYIQAAVACRLNVIVSGGTGSGKTTTLNALSSFIDNSERIL
HHCCHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
TIEDTAELQLQQVHVGRMESRPPNVEGKGAVTQRDCLRNALRMRPDRIIVGETRGEEVID
EEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
MLQAMNTGHDGSMTTIHANNARDACSRLENMVAMAGIEMPLKAVRAQISSAVHLIVQASR
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQDGSRRMVSITEVTGMEGEVISMQEVFRFQRTGLQPDGKILGHFTACGVRSYYSERFRQ
CCCCCCCEEEEEEECCCCCCEECHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
WGYDLPASIYEPMAAQ
CCCCCCHHHHCCCCCC
>Mature Secondary Structure
MFSKYKKAPKGGEVAHITDVAAAPKTDDAPAPENSSAKAQTPAPKVARKPGGSAAAKPAT
CCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
PEAAGAEDKDRKRRLRLDEIKTEMHHRLLDNLNLSALETAKESELRSEITSITSEQLGEM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVVLNREDRQTLNIELFDEVTGLGPLEPLLKDDGVNDILVNGPNRVFIERAGKLQLSDVR
CEEEECCCCCEEEEEEHHHHCCCCCCCHHHHCCCCCEEEECCCCEEEEECCCCEEEECCC
FKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKK
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHH
EKLGVDDLVKFGAFSEEMAAYIQAAVACRLNVIVSGGTGSGKTTTLNALSSFIDNSERIL
HHCCHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
TIEDTAELQLQQVHVGRMESRPPNVEGKGAVTQRDCLRNALRMRPDRIIVGETRGEEVID
EEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
MLQAMNTGHDGSMTTIHANNARDACSRLENMVAMAGIEMPLKAVRAQISSAVHLIVQASR
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQDGSRRMVSITEVTGMEGEVISMQEVFRFQRTGLQPDGKILGHFTACGVRSYYSERFRQ
CCCCCCCEEEEEEECCCCCCEECHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
WGYDLPASIYEPMAAQ
CCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]