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The map label for this gene is fadD [H]

Identifier: 89053158

GI number: 89053158

Start: 637055

End: 638776

Strand: Direct

Name: fadD [H]

Synonym: Jann_0667

Alternate gene names: 89053158

Gene position: 637055-638776 (Clockwise)

Preceding gene: 89053157

Following gene: 89053159

Centisome position: 14.75

GC content: 65.8

Gene sequence:

>1722_bases
GTGATCCGCGAGGATCGGGACGACGGGGCGATCCTGCTGCGCGCGCGGGCCGATATGGGGCCCGTGGCCGAGCGGACGAC
CGATTGGTTGGACCATTGGGCAGAGGTGACGCCGGAGGCCGTGTTTCTGGCGGAGCGTGACGGGCCGGGTTGGCGAGAGG
TCAGCTACGCGGCGGCGCGGGACGAGGCGCGCGCGATTGCGGTTGGGCTGACGGATATGGATCTGGGTCCGGGGCGGCCG
GTGATGGTCGTGACGGGGAATTCTGTTGCCCATGGCCTCCTGGCCCTAGCGTGCCAATATGCAGGCGCGCCGGTGGTGCC
GGTGGCCGAGCAATATGCGGCGCTACCTGCGGCGCGCGGCCAGCTTGACCATATCGCAACGCTCATCCAGCCTGCGGCTG
TCTTCGCGGAGACCCCGATGGAGGAGGTGTTCACGCGGGATAGCCTGGCGGCGGCCCGGCCCCTGACGGGGCAATCACTG
GATGCGCTGGCGCGTGTGGGGGGGCGGCTTGAAGCGCGTATCGGCCCCGACACGGTGGCGAAGATCCTGATGACGTCGGG
CAGCACATCGGCCCCCAAGGGCGTGCTGACGACGCATCGGATGATGTGCGTGAATCAAGCCCAGATCGCGGCGTGCTTGC
CATTCCTGCGCGACCGCCCGCCGGTTCTGGTCGATTGGCTGCCGTGGAACCACGTCTTTGGCGGATCCCATAATTTCAAC
CTTGTGCTTGCCAATGGAGGGTCGCTTTACATCGACGGTGGCAAGCCTGTGCCTGCGTTGGTGGGGCAGACGATTGAGAA
CAATCGCCTTAAACAAGGGACCATCAGCTTCAATGTGCCTGTGGGGTTTGCCGCGCTGCGGGACGCGATGTTGGGCGACC
GGGATTTTGCGCGCGCGTTCTTTCAGGATCTGGACATGCTGTTTTATGCGGGCGCGTCGTTGCCGCAAGACGTCTGGACG
GATCTGGACCGCATGGCCCGTGAGGTGCGGGACGATCTGCCGCTGTTCACCTCGTCCTGGGGGCTGACGGAGACGGCACC
TGCGGCGCTGCTCCAGCATGAGCCGACAGACCGCTCGGGCGTGGTCGGGGTGCCCCTGCCGGGCGTCGACGTGAAGCTGG
TGCCCGCCGAGACGCGCTATGAGGTCCGCGTGCGGGGGCCGTCCGTGTTCAAGGGCTACCACGGGAACGCGGCAAAGACA
GACGAGGCGTTCGACGAGGACGGCTATTTCCGCACCGGCGACGCGATGGCCTTTGTCGATCCATCAGACATGAACCAGGG
TCTTCGGTTTGACGGGCGCATCAGCGAGGATTTCAAGCTGATGTCCGGCACCTGGGTGCGGGCCGCAGCGCTTCGGCTGC
ACTTGCTTGATGGGTTGAAGGGCATCGCCCAGGACGTGGTTCTGGTGGGTGAAGAGCGCGCGGATGTGGGAGTGCTGATC
GTGCCCACGGCAGACATGAGAGAGAGCGCCCGGGACGACGGCGCAGCACTTTTGGCCAACGATCCGACCCCGATCATAGA
GGTCCTGTCCGCCATGAAAGGTGGCAGCGCCGCGCGGGTCACACGGGCGCTGATCCTGTCGCAGCCGCCATCCATTGCCG
ATGGAGAGATCACCGCCAAAGGCAACCTCAATTTCGCCCGGATCCGGGCCGGGCGCGCGGATCTGATCGCGCGGCTCTAT
GATCCCTCGGACCCCGCCTCGATCACTTTGGGAGAGCTGTGA

Upstream 100 bases:

>100_bases
ACGTTGATCGCCGCGTTGCGGCGCGTGGAGGAGAGTGGGGAAGAGGCTTGGGAGGGCCTGCAGAGTGAGCCGGAGATCGA
GGACGTATCAACCACATGAC

Downstream 100 bases:

>100_bases
TGGCCTACATACCTTACGGGGCCTATTGGTCGACGCCCTTTGCCCGCTGGCAGGGGAGCCTGGCGCATCTGAACTCGGTG
CAGTTCGCCGCTCATGTGGG

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; Acyl-CoA synthetase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIREDRDDGAILLRARADMGPVAERTTDWLDHWAEVTPEAVFLAERDGPGWREVSYAAARDEARAIAVGLTDMDLGPGRP
VMVVTGNSVAHGLLALACQYAGAPVVPVAEQYAALPAARGQLDHIATLIQPAAVFAETPMEEVFTRDSLAAARPLTGQSL
DALARVGGRLEARIGPDTVAKILMTSGSTSAPKGVLTTHRMMCVNQAQIAACLPFLRDRPPVLVDWLPWNHVFGGSHNFN
LVLANGGSLYIDGGKPVPALVGQTIENNRLKQGTISFNVPVGFAALRDAMLGDRDFARAFFQDLDMLFYAGASLPQDVWT
DLDRMAREVRDDLPLFTSSWGLTETAPAALLQHEPTDRSGVVGVPLPGVDVKLVPAETRYEVRVRGPSVFKGYHGNAAKT
DEAFDEDGYFRTGDAMAFVDPSDMNQGLRFDGRISEDFKLMSGTWVRAAALRLHLLDGLKGIAQDVVLVGEERADVGVLI
VPTADMRESARDDGAALLANDPTPIIEVLSAMKGGSAARVTRALILSQPPSIADGEITAKGNLNFARIRAGRADLIARLY
DPSDPASITLGEL

Sequences:

>Translated_573_residues
MIREDRDDGAILLRARADMGPVAERTTDWLDHWAEVTPEAVFLAERDGPGWREVSYAAARDEARAIAVGLTDMDLGPGRP
VMVVTGNSVAHGLLALACQYAGAPVVPVAEQYAALPAARGQLDHIATLIQPAAVFAETPMEEVFTRDSLAAARPLTGQSL
DALARVGGRLEARIGPDTVAKILMTSGSTSAPKGVLTTHRMMCVNQAQIAACLPFLRDRPPVLVDWLPWNHVFGGSHNFN
LVLANGGSLYIDGGKPVPALVGQTIENNRLKQGTISFNVPVGFAALRDAMLGDRDFARAFFQDLDMLFYAGASLPQDVWT
DLDRMAREVRDDLPLFTSSWGLTETAPAALLQHEPTDRSGVVGVPLPGVDVKLVPAETRYEVRVRGPSVFKGYHGNAAKT
DEAFDEDGYFRTGDAMAFVDPSDMNQGLRFDGRISEDFKLMSGTWVRAAALRLHLLDGLKGIAQDVVLVGEERADVGVLI
VPTADMRESARDDGAALLANDPTPIIEVLSAMKGGSAARVTRALILSQPPSIADGEITAKGNLNFARIRAGRADLIARLY
DPSDPASITLGEL
>Mature_573_residues
MIREDRDDGAILLRARADMGPVAERTTDWLDHWAEVTPEAVFLAERDGPGWREVSYAAARDEARAIAVGLTDMDLGPGRP
VMVVTGNSVAHGLLALACQYAGAPVVPVAEQYAALPAARGQLDHIATLIQPAAVFAETPMEEVFTRDSLAAARPLTGQSL
DALARVGGRLEARIGPDTVAKILMTSGSTSAPKGVLTTHRMMCVNQAQIAACLPFLRDRPPVLVDWLPWNHVFGGSHNFN
LVLANGGSLYIDGGKPVPALVGQTIENNRLKQGTISFNVPVGFAALRDAMLGDRDFARAFFQDLDMLFYAGASLPQDVWT
DLDRMAREVRDDLPLFTSSWGLTETAPAALLQHEPTDRSGVVGVPLPGVDVKLVPAETRYEVRVRGPSVFKGYHGNAAKT
DEAFDEDGYFRTGDAMAFVDPSDMNQGLRFDGRISEDFKLMSGTWVRAAALRLHLLDGLKGIAQDVVLVGEERADVGVLI
VPTADMRESARDDGAALLANDPTPIIEVLSAMKGGSAARVTRALILSQPPSIADGEITAKGNLNFARIRAGRADLIARLY
DPSDPASITLGEL

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=350, Percent_Identity=25.7142857142857, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI187761343, Length=350, Percent_Identity=25.7142857142857, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788107, Length=281, Percent_Identity=30.6049822064057, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=432, Percent_Identity=24.3055555555556, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=374, Percent_Identity=23.2620320855615, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61474; Mature: 61474

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREDRDDGAILLRARADMGPVAERTTDWLDHWAEVTPEAVFLAERDGPGWREVSYAAAR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHC
DEARAIAVGLTDMDLGPGRPVMVVTGNSVAHGLLALACQYAGAPVVPVAEQYAALPAARG
CCCCEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
QLDHIATLIQPAAVFAETPMEEVFTRDSLAAARPLTGQSLDALARVGGRLEARIGPDTVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCHHHHH
KILMTSGSTSAPKGVLTTHRMMCVNQAQIAACLPFLRDRPPVLVDWLPWNHVFGGSHNFN
HHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
LVLANGGSLYIDGGKPVPALVGQTIENNRLKQGTISFNVPVGFAALRDAMLGDRDFARAF
EEEECCCEEEEECCCCCHHHHCCHHCCCCEECCEEEEECCCCHHHHHHHHCCCHHHHHHH
FQDLDMLFYAGASLPQDVWTDLDRMAREVRDDLPLFTSSWGLTETAPAALLQHEPTDRSG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCCCC
VVGVPLPGVDVKLVPAETRYEVRVRGPSVFKGYHGNAAKTDEAFDEDGYFRTGDAMAFVD
EEECCCCCCCEEEECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCEECCCEEEEEC
PSDMNQGLRFDGRISEDFKLMSGTWVRAAALRLHLLDGLKGIAQDVVLVGEERADVGVLI
HHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEE
VPTADMRESARDDGAALLANDPTPIIEVLSAMKGGSAARVTRALILSQPPSIADGEITAK
EECCHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEC
GNLNFARIRAGRADLIARLYDPSDPASITLGEL
CCCCEEEEECCHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MIREDRDDGAILLRARADMGPVAERTTDWLDHWAEVTPEAVFLAERDGPGWREVSYAAAR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHC
DEARAIAVGLTDMDLGPGRPVMVVTGNSVAHGLLALACQYAGAPVVPVAEQYAALPAARG
CCCCEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
QLDHIATLIQPAAVFAETPMEEVFTRDSLAAARPLTGQSLDALARVGGRLEARIGPDTVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCHHHHH
KILMTSGSTSAPKGVLTTHRMMCVNQAQIAACLPFLRDRPPVLVDWLPWNHVFGGSHNFN
HHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
LVLANGGSLYIDGGKPVPALVGQTIENNRLKQGTISFNVPVGFAALRDAMLGDRDFARAF
EEEECCCEEEEECCCCCHHHHCCHHCCCCEECCEEEEECCCCHHHHHHHHCCCHHHHHHH
FQDLDMLFYAGASLPQDVWTDLDRMAREVRDDLPLFTSSWGLTETAPAALLQHEPTDRSG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCCCC
VVGVPLPGVDVKLVPAETRYEVRVRGPSVFKGYHGNAAKTDEAFDEDGYFRTGDAMAFVD
EEECCCCCCCEEEECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCEECCCEEEEEC
PSDMNQGLRFDGRISEDFKLMSGTWVRAAALRLHLLDGLKGIAQDVVLVGEERADVGVLI
HHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEE
VPTADMRESARDDGAALLANDPTPIIEVLSAMKGGSAARVTRALILSQPPSIADGEITAK
EECCHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEC
GNLNFARIRAGRADLIARLYDPSDPASITLGEL
CCCCEEEEECCHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]