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The map label for this gene is vatD [H]

Identifier: 89053126

GI number: 89053126

Start: 607139

End: 607783

Strand: Reverse

Name: vatD [H]

Synonym: Jann_0635

Alternate gene names: 89053126

Gene position: 607783-607139 (Counterclockwise)

Preceding gene: 89053127

Following gene: 89053125

Centisome position: 14.08

GC content: 63.26

Gene sequence:

>645_bases
ATGACCGGCCCGGACCCCGCAACCCTCGCCCCGATGCTGCCCACCTATGCGGGCACCGTGTTCCTCAAGCCGCTGGCGGA
GGGGCTCGGCAATGTCACCGTGGGCGATTACACCTACTATGACGACCGGGACGAAACGCGCGATTTCTTCACTCGCAACG
TCCTGCACCACTATGATTTCATCGGTGATCACCTCCATATCGGCCCGTTCTGCGCCATTGCCCACGGCGTGCGCATCTTC
ATGAACGGTGGCACGCACGCGATGGATGGGTTCTCGACGTTCCCGTTCAACATCTTCGGGCATGGGTGGGAGGACGGGTT
CGACCCCGCCACCTGGAAAGCCGTCCACAAGGGTGACACCCGTATCGGCCCGGACGTCTGGATCGGCAATTCCGCTACAA
TCATGCCCGGCGTCACCATCGGCGCGGGCGCGATCATTGCCGCCAATGCCACCGTCACCAAGGACGTCGCGCCCTACACA
ATCGCTGCCGGAAATCCAGCGCAGACCGTGAAACATCGGTTTGACGCCGATACCGTGGCGCGTCTTCTGGAGATCGCGTG
GTGGGACTGGCCCGCCGACAAGATCACCCGTAACCTGAACGCCATTCGCGGCGCTGACCTGCCCGCGCTGGAGGCTGCGA
CATGA

Upstream 100 bases:

>100_bases
TGCGCGCGTTGGAAGACGGCTGGGGCCACCAGGATTTCGGCGTCTACGCTATGGTTCGCCAGGGCGGCCAGATCAAAGCG
GGCGACACCGTCGCGGTCGC

Downstream 100 bases:

>100_bases
CCACCCTGCCCTTTCCGCTGGCCGAGGCCCCCGATGCATTGACCGAAGAACGGGGTCGCAAATTGTTCGCGGGCAACACC
GACTTCCTCAAGGGCGTCGT

Product: transferase hexapeptide protein

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTGPDPATLAPMLPTYAGTVFLKPLAEGLGNVTVGDYTYYDDRDETRDFFTRNVLHHYDFIGDHLHIGPFCAIAHGVRIF
MNGGTHAMDGFSTFPFNIFGHGWEDGFDPATWKAVHKGDTRIGPDVWIGNSATIMPGVTIGAGAIIAANATVTKDVAPYT
IAAGNPAQTVKHRFDADTVARLLEIAWWDWPADKITRNLNAIRGADLPALEAAT

Sequences:

>Translated_214_residues
MTGPDPATLAPMLPTYAGTVFLKPLAEGLGNVTVGDYTYYDDRDETRDFFTRNVLHHYDFIGDHLHIGPFCAIAHGVRIF
MNGGTHAMDGFSTFPFNIFGHGWEDGFDPATWKAVHKGDTRIGPDVWIGNSATIMPGVTIGAGAIIAANATVTKDVAPYT
IAAGNPAQTVKHRFDADTVARLLEIAWWDWPADKITRNLNAIRGADLPALEAAT
>Mature_213_residues
TGPDPATLAPMLPTYAGTVFLKPLAEGLGNVTVGDYTYYDDRDETRDFFTRNVLHHYDFIGDHLHIGPFCAIAHGVRIFM
NGGTHAMDGFSTFPFNIFGHGWEDGFDPATWKAVHKGDTRIGPDVWIGNSATIMPGVTIGAGAIIAANATVTKDVAPYTI
AAGNPAQTVKHRFDADTVARLLEIAWWDWPADKITRNLNAIRGADLPALEAAT

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23183; Mature: 23052

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPDPATLAPMLPTYAGTVFLKPLAEGLGNVTVGDYTYYDDRDETRDFFTRNVLHHYDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEECCCHHHHHHHHHHHHHHHHH
IGDHLHIGPFCAIAHGVRIFMNGGTHAMDGFSTFPFNIFGHGWEDGFDPATWKAVHKGDT
HCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCC
RIGPDVWIGNSATIMPGVTIGAGAIIAANATVTKDVAPYTIAAGNPAQTVKHRFDADTVA
CCCCCEEECCCCEECCCCEECCCEEEEECCEEECCCCCEEEECCCHHHHHHHHCCHHHHH
RLLEIAWWDWPADKITRNLNAIRGADLPALEAAT
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TGPDPATLAPMLPTYAGTVFLKPLAEGLGNVTVGDYTYYDDRDETRDFFTRNVLHHYDF
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEECCCHHHHHHHHHHHHHHHHH
IGDHLHIGPFCAIAHGVRIFMNGGTHAMDGFSTFPFNIFGHGWEDGFDPATWKAVHKGDT
HCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCC
RIGPDVWIGNSATIMPGVTIGAGAIIAANATVTKDVAPYTIAAGNPAQTVKHRFDADTVA
CCCCCEEECCCCEECCCCEECCCEEEEECCEEECCCCCEEEECCCHHHHHHHHCCHHHHH
RLLEIAWWDWPADKITRNLNAIRGADLPALEAAT
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 40 {triethanolamine}} 90 {maltose}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]