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The map label for this gene is ispH

Identifier: 89052998

GI number: 89052998

Start: 502885

End: 503844

Strand: Reverse

Name: ispH

Synonym: Jann_0507

Alternate gene names: 89052998

Gene position: 503844-502885 (Counterclockwise)

Preceding gene: 89053000

Following gene: 89052997

Centisome position: 11.67

GC content: 62.19

Gene sequence:

>960_bases
ATGACCCAAACGCCCAAGCCCCCACTGACCATCTATCTCGCCGCCCCGCGCGGGTTCTGCGCCGGTGTCGACCGCGCCAT
CAAGATCGTGGAGATGGCTCTGGAGAAATGGGGCGCGCCGGTCTTTGTGCGGCACGAGATTGTCCATAACAAATACGTGG
TCGATGACCTGAAAGCCAAAGGCGCTGTGTTTGTAGAGGAGCTGTCGGATTGCCCCGATGACCGCCCGGTGATCTTCTCC
GCCCACGGTGTGCCCAAATCCGTGCCCCAGGCCGCCGCCGCGCGTCAGATGGTCTATGTCGATGCCACCTGCCCACTGGT
CTCCAAGGTCCATATCGAGGCGCAGCGCCACTCCGATAACGGCCTCCAGATGGTCATGATCGGCCATAAGGGCCACCCGG
AGACCATTGGGACCATGGGTCAGTTGCCCGATGGCGAAGTGTTGCTGGTTGAAACGGTCGAGGATGTGGCGACGGTTGAG
GTGCGCGACCCGTCCAGGCTGGCATTTGTGACGCAAACCACCCTCTCCGTCGATGACACGATTGAGATTGTGGAGGCCCT
CACCACCCGCTTCCCCGCCATCGTCGGACCCCATAAGGAAGACATCTGCTACGCCACCACCAACCGGCAAGAGGCGGTCA
AAGCCATGGCCCCCAAGGCTGAGGCGATGCTGGTCGTGGGCGCGCCCAATTCCTCCAATTCCAAACGTCTGGTCGAGGTC
GGATCTCGCGCGGGCTGCGCCTATTCGCAACTGGTCCAACGTGCCGCCGACATCGACTGGCGCGCGTTAGGTGACATCAA
AACGCTCGGCATAACCGCGGGCGCATCGGCCCCCGAGGTTCTGATCGAAGAGGTGATCCAGGCCTTTGAAGATCGGTTCG
ACGTCACCCGCGAGTTGGTGGAAACCGCCGTGGAGAACGTCGAATTCAAGGTGCCCCGCGTCCTGCGCGAGACGGCCTAG

Upstream 100 bases:

>100_bases
GCCTCCAAGGCCACAACGCACAAAGCCCCCTGCGCTCATGCACCACTTTACGCGCGCTCCCCTTGCGACTACCTAATTGC
GGACAGGAGTGACCGCCCCC

Downstream 100 bases:

>100_bases
GTCTATCTCTCGCAGGCGATCCCATCGCCGTCGCGATCGAGACGATAAGTATCGGGTCCAACGACGCGGACCGGGCCACG
AACATACCTCGGTCCATTCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTQTPKPPLTIYLAAPRGFCAGVDRAIKIVEMALEKWGAPVFVRHEIVHNKYVVDDLKAKGAVFVEELSDCPDDRPVIFS
AHGVPKSVPQAAAARQMVYVDATCPLVSKVHIEAQRHSDNGLQMVMIGHKGHPETIGTMGQLPDGEVLLVETVEDVATVE
VRDPSRLAFVTQTTLSVDDTIEIVEALTTRFPAIVGPHKEDICYATTNRQEAVKAMAPKAEAMLVVGAPNSSNSKRLVEV
GSRAGCAYSQLVQRAADIDWRALGDIKTLGITAGASAPEVLIEEVIQAFEDRFDVTRELVETAVENVEFKVPRVLRETA

Sequences:

>Translated_319_residues
MTQTPKPPLTIYLAAPRGFCAGVDRAIKIVEMALEKWGAPVFVRHEIVHNKYVVDDLKAKGAVFVEELSDCPDDRPVIFS
AHGVPKSVPQAAAARQMVYVDATCPLVSKVHIEAQRHSDNGLQMVMIGHKGHPETIGTMGQLPDGEVLLVETVEDVATVE
VRDPSRLAFVTQTTLSVDDTIEIVEALTTRFPAIVGPHKEDICYATTNRQEAVKAMAPKAEAMLVVGAPNSSNSKRLVEV
GSRAGCAYSQLVQRAADIDWRALGDIKTLGITAGASAPEVLIEEVIQAFEDRFDVTRELVETAVENVEFKVPRVLRETA
>Mature_318_residues
TQTPKPPLTIYLAAPRGFCAGVDRAIKIVEMALEKWGAPVFVRHEIVHNKYVVDDLKAKGAVFVEELSDCPDDRPVIFSA
HGVPKSVPQAAAARQMVYVDATCPLVSKVHIEAQRHSDNGLQMVMIGHKGHPETIGTMGQLPDGEVLLVETVEDVATVEV
RDPSRLAFVTQTTLSVDDTIEIVEALTTRFPAIVGPHKEDICYATTNRQEAVKAMAPKAEAMLVVGAPNSSNSKRLVEVG
SRAGCAYSQLVQRAADIDWRALGDIKTLGITAGASAPEVLIEEVIQAFEDRFDVTRELVETAVENVEFKVPRVLRETA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=52.258064516129, Blast_Score=328, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34687; Mature: 34556

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTPKPPLTIYLAAPRGFCAGVDRAIKIVEMALEKWGAPVFVRHEIVHNKYVVDDLKAK
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEHHHCCC
GAVFVEELSDCPDDRPVIFSAHGVPKSVPQAAAARQMVYVDATCPLVSKVHIEAQRHSDN
CCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCEEEEEECCCCCHHEEEEEEECCCCC
GLQMVMIGHKGHPETIGTMGQLPDGEVLLVETVEDVATVEVRDPSRLAFVTQTTLSVDDT
CEEEEEEECCCCCCHHCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEEEEECCCHHH
IEIVEALTTRFPAIVGPHKEDICYATTNRQEAVKAMAPKAEAMLVVGAPNSSNSKRLVEV
HHHHHHHHHHCCHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEC
GSRAGCAYSQLVQRAADIDWRALGDIKTLGITAGASAPEVLIEEVIQAFEDRFDVTRELV
CCCCCHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ETAVENVEFKVPRVLRETA
HHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure 
TQTPKPPLTIYLAAPRGFCAGVDRAIKIVEMALEKWGAPVFVRHEIVHNKYVVDDLKAK
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEHHHCCC
GAVFVEELSDCPDDRPVIFSAHGVPKSVPQAAAARQMVYVDATCPLVSKVHIEAQRHSDN
CCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCEEEEEECCCCCHHEEEEEEECCCCC
GLQMVMIGHKGHPETIGTMGQLPDGEVLLVETVEDVATVEVRDPSRLAFVTQTTLSVDDT
CEEEEEEECCCCCCHHCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEEEEECCCHHH
IEIVEALTTRFPAIVGPHKEDICYATTNRQEAVKAMAPKAEAMLVVGAPNSSNSKRLVEV
HHHHHHHHHHCCHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEC
GSRAGCAYSQLVQRAADIDWRALGDIKTLGITAGASAPEVLIEEVIQAFEDRFDVTRELV
CCCCCHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
ETAVENVEFKVPRVLRETA
HHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA