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The map label for this gene is mutY [H]

Identifier: 89052886

GI number: 89052886

Start: 398123

End: 399160

Strand: Reverse

Name: mutY [H]

Synonym: Jann_0395

Alternate gene names: 89052886

Gene position: 399160-398123 (Counterclockwise)

Preceding gene: 89052890

Following gene: 89052885

Centisome position: 9.24

GC content: 64.07

Gene sequence:

>1038_bases
TTGCTGGACTGGTACGACGTACACGCGCGCGATTTGCCCTGGCGCGTGCCCCCGTTAAGCGGGCTGGACGCGGACCCTTA
CCGCGTGTGGCTCAGCGAGATCATGTTGCAGCAAACCACCGTGGCCGCCGTGAAAGCGTATTTTCAGCGGTTCACGGCGC
TCTGGCCCACGGTTGGTGATCTGGCCGCAGCAGAGGATGCGGCCGTGATGGGTGAATGGGCGGGTCTTGGTTACTACGCG
CGTGCCCGGAATTTGCTGAAATGCGCCCGCGTGGTGGTGGAGGAACACGGCGGGCAGTTCCCAAGGACGGAGGCAGAGCT
GCTGGAATTGCCCGGGATCGGGCCCTACACAGCCGCCGCCGTCGCCTCCATCGCGTTTCAACAACCCGCCCCGGTCATGG
ATGGCAATATCGAACGGGTCATGGCCCGGCTCTTCGCGGTGGAGGACCCGCTGCCGGGCTGCAAATCTGTTCTGAAAGAA
CACGCGACCCGCCTGACGCCCAATGATCGCCCCGGTGATCATGCGCAGGCGCTGATGGATCTGGGCGCCACGATCTGTAC
GCCGAAAAACCCTGCCTGTGGGATCTGCCCGGTGATGGAGGCCTGTACCGCCCACAAACGGGGCATCGCTGCCGACCTTC
CAAAGAAAGCCCCCAAGAAAGCCAAGCCCACCCGCCTTGGCTATGTCTATGTGGCGCGCCAGGAGGACGGGTCAGTGCTG
CTGGAAACCCGCGAGGAAAAGGGATTGTTGGGCGGCATGTTGGCCTTCCCCACCAGCGATTGGTCCGAAGCGCCGACCGA
CGTGCCACCCTTCGCCGCCGATTGGCACGACCCGGGCCTGGAGGTGCGCCATACCTTCACCCATTTCCACCTGCGCCTCG
CCCTGCGTGTCACCAACGCGGCATCGGACACGTCGCCGCAGCGGGGCGAATTCCTGCCGCGCGCGCTATTCAAACCGTCG
TCCCTGCCTACACTGATGCGCAAGGCGCACGATCTAAGCGTGGGACACTTCACCGGAGATGATCATGATCTCGGCTGA

Upstream 100 bases:

>100_bases
ATCCTTTGGCTGAGCGCACTCTATGCCTTCACGGGATGCTAACCAAGTGCAAGCCACACAGGATGGATAACGGGTGCGTG
AACAGCTGATCGGAGGAGCC

Downstream 100 bases:

>100_bases
CAAATTCGCAAAAATCCACCGTGCCCTGCCCTTCTGGGCAAGCCTCGCGCTGATCCCACTGATCGCCTTTGTTGCCACGA
AAGGGGGCTGGTGGCTGGTG

Product: A/G-specific DNA-adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLDWYDVHARDLPWRVPPLSGLDADPYRVWLSEIMLQQTTVAAVKAYFQRFTALWPTVGDLAAAEDAAVMGEWAGLGYYA
RARNLLKCARVVVEEHGGQFPRTEAELLELPGIGPYTAAAVASIAFQQPAPVMDGNIERVMARLFAVEDPLPGCKSVLKE
HATRLTPNDRPGDHAQALMDLGATICTPKNPACGICPVMEACTAHKRGIAADLPKKAPKKAKPTRLGYVYVARQEDGSVL
LETREEKGLLGGMLAFPTSDWSEAPTDVPPFAADWHDPGLEVRHTFTHFHLRLALRVTNAASDTSPQRGEFLPRALFKPS
SLPTLMRKAHDLSVGHFTGDDHDLG

Sequences:

>Translated_345_residues
MLDWYDVHARDLPWRVPPLSGLDADPYRVWLSEIMLQQTTVAAVKAYFQRFTALWPTVGDLAAAEDAAVMGEWAGLGYYA
RARNLLKCARVVVEEHGGQFPRTEAELLELPGIGPYTAAAVASIAFQQPAPVMDGNIERVMARLFAVEDPLPGCKSVLKE
HATRLTPNDRPGDHAQALMDLGATICTPKNPACGICPVMEACTAHKRGIAADLPKKAPKKAKPTRLGYVYVARQEDGSVL
LETREEKGLLGGMLAFPTSDWSEAPTDVPPFAADWHDPGLEVRHTFTHFHLRLALRVTNAASDTSPQRGEFLPRALFKPS
SLPTLMRKAHDLSVGHFTGDDHDLG
>Mature_345_residues
MLDWYDVHARDLPWRVPPLSGLDADPYRVWLSEIMLQQTTVAAVKAYFQRFTALWPTVGDLAAAEDAAVMGEWAGLGYYA
RARNLLKCARVVVEEHGGQFPRTEAELLELPGIGPYTAAAVASIAFQQPAPVMDGNIERVMARLFAVEDPLPGCKSVLKE
HATRLTPNDRPGDHAQALMDLGATICTPKNPACGICPVMEACTAHKRGIAADLPKKAPKKAKPTRLGYVYVARQEDGSVL
LETREEKGLLGGMLAFPTSDWSEAPTDVPPFAADWHDPGLEVRHTFTHFHLRLALRVTNAASDTSPQRGEFLPRALFKPS
SLPTLMRKAHDLSVGHFTGDDHDLG

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298650, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI115298654, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI115298652, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI115298648, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI6912520, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI190358497, Length=406, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1789331, Length=300, Percent_Identity=44.6666666666667, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1787920, Length=187, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 37762; Mature: 37762

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDWYDVHARDLPWRVPPLSGLDADPYRVWLSEIMLQQTTVAAVKAYFQRFTALWPTVGD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAAAEDAAVMGEWAGLGYYARARNLLKCARVVVEEHGGQFPRTEAELLELPGIGPYTAAA
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHH
VASIAFQQPAPVMDGNIERVMARLFAVEDPLPGCKSVLKEHATRLTPNDRPGDHAQALMD
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LGATICTPKNPACGICPVMEACTAHKRGIAADLPKKAPKKAKPTRLGYVYVARQEDGSVL
CCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEE
LETREEKGLLGGMLAFPTSDWSEAPTDVPPFAADWHDPGLEVRHTFTHFHLRLALRVTNA
EEECHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEEECC
ASDTSPQRGEFLPRALFKPSSLPTLMRKAHDLSVGHFTGDDHDLG
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDWYDVHARDLPWRVPPLSGLDADPYRVWLSEIMLQQTTVAAVKAYFQRFTALWPTVGD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LAAAEDAAVMGEWAGLGYYARARNLLKCARVVVEEHGGQFPRTEAELLELPGIGPYTAAA
HHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHH
VASIAFQQPAPVMDGNIERVMARLFAVEDPLPGCKSVLKEHATRLTPNDRPGDHAQALMD
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LGATICTPKNPACGICPVMEACTAHKRGIAADLPKKAPKKAKPTRLGYVYVARQEDGSVL
CCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEE
LETREEKGLLGGMLAFPTSDWSEAPTDVPPFAADWHDPGLEVRHTFTHFHLRLALRVTNA
EEECHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEEECC
ASDTSPQRGEFLPRALFKPSSLPTLMRKAHDLSVGHFTGDDHDLG
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]