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The map label for this gene is rppH [H]

Identifier: 89052873

GI number: 89052873

Start: 388371

End: 388832

Strand: Direct

Name: rppH [H]

Synonym: Jann_0382

Alternate gene names: 89052873

Gene position: 388371-388832 (Clockwise)

Preceding gene: 89052872

Following gene: 89052874

Centisome position: 8.99

GC content: 60.39

Gene sequence:

>462_bases
GTGGCTGATCTACCTTATCGCCCGTGCGCGGGGGTCGTTTTGACCAACGCGGACGGGCGTATCTTTGCGGGACAGCGCGC
AGGGTTCGACACGCCCGCCTGGCAGATGCCCCAGGGCGGGCTCGATAAGGGGGAAGACCCCTTGGACGCCGCTTACCGAG
AGCTGGAAGAAGAAACCGGCGTGGGCCGCGACCACGTCACGTTTGTGGCACAAACGACCGATTGGCTGACCTATGACTTC
CCGCCCGAGCTGGCGCTGGGGCGATGGAAGGGCAAATATGGCGGCCAGAAGCAGATGTGGGCGCATCTTCAACTGGACGC
GCCCGACAGCGTCATTAACCTCACCCACAAAGACGTGGAATTCAGCGATTGGCGGTGGATGACAAAGCGGGATATCCTGA
CTGCCATCGTGCCCTTCAAGCGTGGTATCTATAAGGCCATCTTCAAGGAGTTCGGTCTATGA

Upstream 100 bases:

>100_bases
TGCCATTGCCGAGGCGACCGCAGCCCTGCGCAACGAAGATTATCAGCTGGCCTATGCTATTGATATCCTGACGGGTCTTG
GCGCGTTAGGTCCGAACTGA

Downstream 100 bases:

>100_bases
TCCGCTGCGCCGCATTTGCCCTGTCCGTTTTTGCCGCGGGCCCCGCCGTCGCGTTGTCCTGCGCGGTACCGTCCGTTGCA
GACAGCTATGGCGGGGCTGC

Product: NUDIX hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MADLPYRPCAGVVLTNADGRIFAGQRAGFDTPAWQMPQGGLDKGEDPLDAAYRELEEETGVGRDHVTFVAQTTDWLTYDF
PPELALGRWKGKYGGQKQMWAHLQLDAPDSVINLTHKDVEFSDWRWMTKRDILTAIVPFKRGIYKAIFKEFGL

Sequences:

>Translated_153_residues
MADLPYRPCAGVVLTNADGRIFAGQRAGFDTPAWQMPQGGLDKGEDPLDAAYRELEEETGVGRDHVTFVAQTTDWLTYDF
PPELALGRWKGKYGGQKQMWAHLQLDAPDSVINLTHKDVEFSDWRWMTKRDILTAIVPFKRGIYKAIFKEFGL
>Mature_152_residues
ADLPYRPCAGVVLTNADGRIFAGQRAGFDTPAWQMPQGGLDKGEDPLDAAYRELEEETGVGRDHVTFVAQTTDWLTYDFP
PELALGRWKGKYGGQKQMWAHLQLDAPDSVINLTHKDVEFSDWRWMTKRDILTAIVPFKRGIYKAIFKEFGL

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789194, Length=150, Percent_Identity=41.3333333333333, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 17350; Mature: 17219

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLPYRPCAGVVLTNADGRIFAGQRAGFDTPAWQMPQGGLDKGEDPLDAAYRELEEETG
CCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
VGRDHVTFVAQTTDWLTYDFPPELALGRWKGKYGGQKQMWAHLQLDAPDSVINLTHKDVE
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCC
FSDWRWMTKRDILTAIVPFKRGIYKAIFKEFGL
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADLPYRPCAGVVLTNADGRIFAGQRAGFDTPAWQMPQGGLDKGEDPLDAAYRELEEETG
CCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
VGRDHVTFVAQTTDWLTYDFPPELALGRWKGKYGGQKQMWAHLQLDAPDSVINLTHKDVE
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCC
FSDWRWMTKRDILTAIVPFKRGIYKAIFKEFGL
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA