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The map label for this gene is 89052838

Identifier: 89052838

GI number: 89052838

Start: 351543

End: 352382

Strand: Reverse

Name: 89052838

Synonym: Jann_0347

Alternate gene names: NA

Gene position: 352382-351543 (Counterclockwise)

Preceding gene: 89052839

Following gene: 89052832

Centisome position: 8.16

GC content: 61.67

Gene sequence:

>840_bases
ATGAAAGACGACCGTAAACCGTTCCGCGATGCCCATCAGGACATCGAATACGCCAAGACCTTGCCCGACACCCCGCAGGC
GCAATCACCTGCCTACCGGCTCGCCTTCGCCGATCCGGATTTCCTGTGTCGCGACGAGTTGCGGCCGGTGCGGTTGCAGC
TTGAACTGCTCAAGCCCGAACTGGGACTGGATGAACACGGGATCAAATCAACCATTGTTCTGTTCGGGGGCGCGCGCATT
CCGCGGCCTGAGGATCGCGACGGCGCACGCAGCGATGCCTTGCGAGACATGTCGAAATACTATGAGGAGGCGCGAGCATT
CGCCCATGCGATCACGGAGCGGTCCGTCGCCAGCAATTGCACTGAAAATGTTGTCGTCACCGGTGGCGGCCCGGGGGTAA
TGGAAGCGGGCAATCGCGGCGCACAGGATGCGGGCGGCTGTTCCATCGGGCTCAACATCGTGCTCCCGCACGAGCAGGAG
CCGAACCACTACATCACGCCCGAATTGTGCTTCAATTTCCACTACTTCGCGATCCGCAAGATGCACTTCCTGATGCGCGC
CAAGGCCATCGCCGTCTTTCCCGGTGGCTTTGGCACCATGGACGAGACATTCGAGGCGCTGACCCTGATCCAGACCGGGC
GGATGGAGCAGGTCCCCGTCCTGCTGTTCGGAGAGGCTTTCTGGCGCGAGATCATCAACTGGGATGCGCTGGTGCGTGCG
GGAACGATTTCGGCCGAGGACCTGAACCTGTTTCGGTTCGTGGAAACGGCTCAGGACGCGCTGGATGTGATCGACGGCTG
GACCCTTTCGGGCAAACGCGGCGAGATCCCGGGGCGATAG

Upstream 100 bases:

>100_bases
CCTTCGTGCAGCCATCTGGGTTGAACAACCGCGCGCGTTTCGCCCCTTGGGGCCTGGCACTGTCCACGATAGGATCAATC
GAAATGTGCCCGGAGGCCCT

Downstream 100 bases:

>100_bases
AGAACGACCGGCGCACCTGATCAGGGGTGCGCCGGGTTCATCTGATGACGGGCAGGGTTCAGTAGCGAATGTTGAACTGA
ACGCTCACGTTCGTCTGGGT

Product: hypothetical protein

Products: (2S)-2-isopropyl-3-oxosuccinate; NADH; H+

Alternate protein names: None

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKDDRKPFRDAHQDIEYAKTLPDTPQAQSPAYRLAFADPDFLCRDELRPVRLQLELLKPELGLDEHGIKSTIVLFGGARI
PRPEDRDGARSDALRDMSKYYEEARAFAHAITERSVASNCTENVVVTGGGPGVMEAGNRGAQDAGGCSIGLNIVLPHEQE
PNHYITPELCFNFHYFAIRKMHFLMRAKAIAVFPGGFGTMDETFEALTLIQTGRMEQVPVLLFGEAFWREIINWDALVRA
GTISAEDLNLFRFVETAQDALDVIDGWTLSGKRGEIPGR

Sequences:

>Translated_279_residues
MKDDRKPFRDAHQDIEYAKTLPDTPQAQSPAYRLAFADPDFLCRDELRPVRLQLELLKPELGLDEHGIKSTIVLFGGARI
PRPEDRDGARSDALRDMSKYYEEARAFAHAITERSVASNCTENVVVTGGGPGVMEAGNRGAQDAGGCSIGLNIVLPHEQE
PNHYITPELCFNFHYFAIRKMHFLMRAKAIAVFPGGFGTMDETFEALTLIQTGRMEQVPVLLFGEAFWREIINWDALVRA
GTISAEDLNLFRFVETAQDALDVIDGWTLSGKRGEIPGR
>Mature_279_residues
MKDDRKPFRDAHQDIEYAKTLPDTPQAQSPAYRLAFADPDFLCRDELRPVRLQLELLKPELGLDEHGIKSTIVLFGGARI
PRPEDRDGARSDALRDMSKYYEEARAFAHAITERSVASNCTENVVVTGGGPGVMEAGNRGAQDAGGCSIGLNIVLPHEQE
PNHYITPELCFNFHYFAIRKMHFLMRAKAIAVFPGGFGTMDETFEALTLIQTGRMEQVPVLLFGEAFWREIINWDALVRA
GTISAEDLNLFRFVETAQDALDVIDGWTLSGKRGEIPGR

Specific function: Unknown

COG id: COG1611

COG function: function code R; Predicted Rossmann fold nucleotide-binding protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.85

Molecular weight: Translated: 31140; Mature: 31140

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDDRKPFRDAHQDIEYAKTLPDTPQAQSPAYRLAFADPDFLCRDELRPVRLQLELLKPE
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCCCCEEEEEEECCCC
LGLDEHGIKSTIVLFGGARIPRPEDRDGARSDALRDMSKYYEEARAFAHAITERSVASNC
CCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TENVVVTGGGPGVMEAGNRGAQDAGGCSIGLNIVLPHEQEPNHYITPELCFNFHYFAIRK
CCCEEEECCCCCHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEECHHHHHHHHHHHHHH
MHFLMRAKAIAVFPGGFGTMDETFEALTLIQTGRMEQVPVLLFGEAFWREIINWDALVRA
HHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHCHHHHHHC
GTISAEDLNLFRFVETAQDALDVIDGWTLSGKRGEIPGR
CCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure
MKDDRKPFRDAHQDIEYAKTLPDTPQAQSPAYRLAFADPDFLCRDELRPVRLQLELLKPE
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCCCCEEEEEEECCCC
LGLDEHGIKSTIVLFGGARIPRPEDRDGARSDALRDMSKYYEEARAFAHAITERSVASNC
CCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TENVVVTGGGPGVMEAGNRGAQDAGGCSIGLNIVLPHEQEPNHYITPELCFNFHYFAIRK
CCCEEEECCCCCHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEECHHHHHHHHHHHHHH
MHFLMRAKAIAVFPGGFGTMDETFEALTLIQTGRMEQVPVLLFGEAFWREIINWDALVRA
HHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHCHHHHHHC
GTISAEDLNLFRFVETAQDALDVIDGWTLSGKRGEIPGR
CCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (2R,3S)-3-isopropylmalate; NAD+

Specific reaction: (2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA