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The map label for this gene is dapF [H]

Identifier: 89052809

GI number: 89052809

Start: 325644

End: 326489

Strand: Direct

Name: dapF [H]

Synonym: Jann_0318

Alternate gene names: 89052809

Gene position: 325644-326489 (Clockwise)

Preceding gene: 89052806

Following gene: 89052810

Centisome position: 7.54

GC content: 64.07

Gene sequence:

>846_bases
ATGCGGCAAATGCCTGATGAGACACCATATCCCGGCCTGCCCTTTTTGAAGATGCATGGCCTTGGCAACGATTTCGTTGT
GATCGATGCCCGTGGGGTGGCGCGCGCGATGACGCCTGCCCTCGCGCGCGCATTGGGTGATCGGCATCGGGGTGTGGGGT
TTGACCAGCTCGCTGTGATTGAGACGGGCACCGACAGTGATGTCGAACTGACGTTCTGGAACGCCGATGGATCGACCGCC
GGGGCCTGTGGAAACGCGACCCGCTGCATCGCCCGACGCGAGATGGAGCTGACCGGAAAGCACCGCATCACCCTGCGCAC
CGAACGGGGCCTTCTTGAGGCTATCGATGTGGGCGACGGTCTGACCAGTGTGAATATGGGCGAACCGGTGATGGGGTGGC
GCGACATCCCCCTCAAGGGGGCGTGCGATACACTGAACCTGCCCATCGACGGCACGCCGGTCGCCACCGGCATGGGCAAT
CCCCACTGCACCTTCTTCGTGGAGGACGCCGACGCCATCGACCTGGAAACGCTCGGCGCGAAATACGAACGCCATCCGCT
GTTTCCCCAACGCACCAACGTGCAGTTTGCCCATGTGGTCGGCCCCGATCATCTGCGTATGCGGGTTTGGGAACGCGGTG
TTGGTGTCACACTGGCGTCGGGCTCGTCGTCCTGTGCCGTCGCAGTGTCCGCGGCGCGTCGGGGGCTGACCGGCCGAAAG
GTTCGCCTCACCCTTGATGGGGGCGAGATCAGCGTGGATTGGCGCAGCGATGGCGTCTGGATGACAGGCCCCACCGCCCA
CGTCTTTTCCGGGGAACTGACCCCGGCCTTCCTGGACGCACTATGA

Upstream 100 bases:

>100_bases
CCGTTGATTAGGGGCGGGCCGCAGAGTCGTCAAGTCAAAATCCTGCCGGGATTGGGGAAAGCAACCATAGGGCCTTTGAA
CCACGCGTCGCATCTTCTAT

Downstream 100 bases:

>100_bases
GCGCGCCAGTGTTCCACACGCTTGGCTGCCGTCTCAACGCCTATGAGACCGAGGCAATGCGTGAGATGACGGCTCAGGCG
GGCCTCGAAAACGCCGTCGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRQMPDETPYPGLPFLKMHGLGNDFVVIDARGVARAMTPALARALGDRHRGVGFDQLAVIETGTDSDVELTFWNADGSTA
GACGNATRCIARREMELTGKHRITLRTERGLLEAIDVGDGLTSVNMGEPVMGWRDIPLKGACDTLNLPIDGTPVATGMGN
PHCTFFVEDADAIDLETLGAKYERHPLFPQRTNVQFAHVVGPDHLRMRVWERGVGVTLASGSSSCAVAVSAARRGLTGRK
VRLTLDGGEISVDWRSDGVWMTGPTAHVFSGELTPAFLDAL

Sequences:

>Translated_281_residues
MRQMPDETPYPGLPFLKMHGLGNDFVVIDARGVARAMTPALARALGDRHRGVGFDQLAVIETGTDSDVELTFWNADGSTA
GACGNATRCIARREMELTGKHRITLRTERGLLEAIDVGDGLTSVNMGEPVMGWRDIPLKGACDTLNLPIDGTPVATGMGN
PHCTFFVEDADAIDLETLGAKYERHPLFPQRTNVQFAHVVGPDHLRMRVWERGVGVTLASGSSSCAVAVSAARRGLTGRK
VRLTLDGGEISVDWRSDGVWMTGPTAHVFSGELTPAFLDAL
>Mature_281_residues
MRQMPDETPYPGLPFLKMHGLGNDFVVIDARGVARAMTPALARALGDRHRGVGFDQLAVIETGTDSDVELTFWNADGSTA
GACGNATRCIARREMELTGKHRITLRTERGLLEAIDVGDGLTSVNMGEPVMGWRDIPLKGACDTLNLPIDGTPVATGMGN
PHCTFFVEDADAIDLETLGAKYERHPLFPQRTNVQFAHVVGPDHLRMRVWERGVGVTLASGSSSCAVAVSAARRGLTGRK
VRLTLDGGEISVDWRSDGVWMTGPTAHVFSGELTPAFLDAL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=270, Percent_Identity=41.8518518518518, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30224; Mature: 30224

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQMPDETPYPGLPFLKMHGLGNDFVVIDARGVARAMTPALARALGDRHRGVGFDQLAVI
CCCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ETGTDSDVELTFWNADGSTAGACGNATRCIARREMELTGKHRITLRTERGLLEAIDVGDG
ECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCEEECCCEEEEEEECCCCEEEEECCCC
LTSVNMGEPVMGWRDIPLKGACDTLNLPIDGTPVATGMGNPHCTFFVEDADAIDLETLGA
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCEEEEEECCCCCCCHHHHCC
KYERHPLFPQRTNVQFAHVVGPDHLRMRVWERGVGVTLASGSSSCAVAVSAARRGLTGRK
CHHCCCCCCCCCCEEEEEEECCCHHEEEEECCCCCEEEECCCCCEEEEEEHHHCCCCCCE
VRLTLDGGEISVDWRSDGVWMTGPTAHVFSGELTPAFLDAL
EEEEECCCEEEEEEECCCEEEECCCCEEECCCCCHHHHHCC
>Mature Secondary Structure
MRQMPDETPYPGLPFLKMHGLGNDFVVIDARGVARAMTPALARALGDRHRGVGFDQLAVI
CCCCCCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ETGTDSDVELTFWNADGSTAGACGNATRCIARREMELTGKHRITLRTERGLLEAIDVGDG
ECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCEEECCCEEEEEEECCCCEEEEECCCC
LTSVNMGEPVMGWRDIPLKGACDTLNLPIDGTPVATGMGNPHCTFFVEDADAIDLETLGA
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCEEEEEECCCCCCCHHHHCC
KYERHPLFPQRTNVQFAHVVGPDHLRMRVWERGVGVTLASGSSSCAVAVSAARRGLTGRK
CHHCCCCCCCCCCEEEEEEECCCHHEEEEECCCCCEEEECCCCCEEEEEEHHHCCCCCCE
VRLTLDGGEISVDWRSDGVWMTGPTAHVFSGELTPAFLDAL
EEEEECCCEEEEEEECCCEEEECCCCEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA