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The map label for this gene is grpE [H]

Identifier: 89052699

GI number: 89052699

Start: 199400

End: 199966

Strand: Direct

Name: grpE [H]

Synonym: Jann_0208

Alternate gene names: 89052699

Gene position: 199400-199966 (Clockwise)

Preceding gene: 89052698

Following gene: 89052701

Centisome position: 4.62

GC content: 61.2

Gene sequence:

>567_bases
ATGGGCGAGAGAGACGACGAATTTTATGAAGATCAGACGGCGGATGCTCAGGACAAGGCGGATGTTTCGCTTGCGCCAGA
GGATATGTCTGGCACGGACGCTGTGATCGAGGCGCTTGAGGCGGAGCGGGATGAGCTGCGCGACCGGATGCTGCGCGCGA
TGGCCGAGGCTGAGAATATGCGCAAACGCGCTGACCGGGACCGCCGCGAGGCAGAGCAATACGGCGGCTCCAAGCTGTCC
CGCGATTTGCTGCCCGTCTACGACAACATGCGCCGCGCGTTAGATGCTGCGGATGACGCGACCCGTGAAGCGGCCAGCGG
TCTGGTCGAAGGCGTGGAACTGACGATGCGCGAGCTGATCAGCATTTTCGGCAAGCACGGCATCGTGCCCGTTGTCCCCG
AAGTGGGCGAACGCTTTAATGCCCAAGAACATGAGGCGATGTTTGAAGCGCCCGTTCCCGGCACCAAAGCGGGCGATATC
ATTCAGGTGATGTCCGAAGGATTCCTGCTGCACGATCGCCTTTTGCGTCCCGCGCAGGTCGGTGTTTCGTCGACGCCAGC
TAGCTAA

Upstream 100 bases:

>100_bases
CCGTCTGAACTACGGACGCATCGTTCCGATTGTGGATTACACGGCGCAGCTAGTTGGACGGCTGATTTCCGACCGGTCCT
GAGAGGCCAAAGAGGGTATA

Downstream 100 bases:

>100_bases
GCTCAGCCTTCAATTCATAGAGCAAATTCAGTGCCTCGCGCGCCGTCATCTCATCGGGATGTACGGCGCGGAGTTTCTCT
TCGACCAAAGACGGCGCCGA

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MGERDDEFYEDQTADAQDKADVSLAPEDMSGTDAVIEALEAERDELRDRMLRAMAEAENMRKRADRDRREAEQYGGSKLS
RDLLPVYDNMRRALDAADDATREAASGLVEGVELTMRELISIFGKHGIVPVVPEVGERFNAQEHEAMFEAPVPGTKAGDI
IQVMSEGFLLHDRLLRPAQVGVSSTPAS

Sequences:

>Translated_188_residues
MGERDDEFYEDQTADAQDKADVSLAPEDMSGTDAVIEALEAERDELRDRMLRAMAEAENMRKRADRDRREAEQYGGSKLS
RDLLPVYDNMRRALDAADDATREAASGLVEGVELTMRELISIFGKHGIVPVVPEVGERFNAQEHEAMFEAPVPGTKAGDI
IQVMSEGFLLHDRLLRPAQVGVSSTPAS
>Mature_187_residues
GERDDEFYEDQTADAQDKADVSLAPEDMSGTDAVIEALEAERDELRDRMLRAMAEAENMRKRADRDRREAEQYGGSKLSR
DLLPVYDNMRRALDAADDATREAASGLVEGVELTMRELISIFGKHGIVPVVPEVGERFNAQEHEAMFEAPVPGTKAGDII
QVMSEGFLLHDRLLRPAQVGVSSTPAS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=36.6666666666667, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI40255109, Length=151, Percent_Identity=29.1390728476821, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788967, Length=141, Percent_Identity=34.0425531914894, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=34.2105263157895, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=185, Percent_Identity=33.5135135135135, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653432, Length=179, Percent_Identity=31.2849162011173, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20793; Mature: 20662

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERDDEFYEDQTADAQDKADVSLAPEDMSGTDAVIEALEAERDELRDRMLRAMAEAENM
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRADRDRREAEQYGGSKLSRDLLPVYDNMRRALDAADDATREAASGLVEGVELTMRELI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFGKHGIVPVVPEVGERFNAQEHEAMFEAPVPGTKAGDIIQVMSEGFLLHDRLLRPAQV
HHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHC
GVSSTPAS
CCCCCCCC
>Mature Secondary Structure 
GERDDEFYEDQTADAQDKADVSLAPEDMSGTDAVIEALEAERDELRDRMLRAMAEAENM
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRADRDRREAEQYGGSKLSRDLLPVYDNMRRALDAADDATREAASGLVEGVELTMRELI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFGKHGIVPVVPEVGERFNAQEHEAMFEAPVPGTKAGDIIQVMSEGFLLHDRLLRPAQV
HHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHC
GVSSTPAS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA