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The map label for this gene is leuD

Identifier: 89052614

GI number: 89052614

Start: 113445

End: 114050

Strand: Direct

Name: leuD

Synonym: Jann_0123

Alternate gene names: 89052614

Gene position: 113445-114050 (Clockwise)

Preceding gene: 89052613

Following gene: 89052615

Centisome position: 2.63

GC content: 58.25

Gene sequence:

>606_bases
ATGGACAAGTTCACCACATTGACCGGCATTGCCGCTCCGATGCCCCTGATCAACGTCGATACCGACATGATCATCCCCAA
GCAATTCCTGAAGACGATCAAACGCTCGGGCCTTGGGGCGAACCTGTTTGACGAGATGCGCTTTGACGATGATGGCAACG
AGATCCCCGATTTCGTGCTGAACAAACCTGCATATCGTGACGCGCAGATCCTTGTCGCGGGCGATAACTTCGGCTGTGGG
TCGTCGCGGGAGCACGCCCCATGGGCGCTTCTGGATTTCGGCATCCGCTGCGTGATCGCCACGTCCTTCGCGGATATTTT
CTACAACAACTGCTTCAAGAACGGCATCCTGCCCATCGTCCTGCTGCAAGAGCAGGTCGATATGCTGATGGATGATGCCG
AGCGCGGGGCGAATGCGGTCGTCTCCGTCGATCTGGAAAGCCAGACAATCACCGGCCCCGATGGTGGCACAATCAGCTTC
GAGGTCGATGCATTCCGCAAGCATTGCCTGCTGAACGGCCTTGATGACATCGGTCTGACGCTGGAAAAGGCCCCGTCGAT
CAAGGCGTTTGAGGCCGAAGCCGCTCAGGCCCGCCCCTGGGTCTAG

Upstream 100 bases:

>100_bases
TACAACAAGAACCGCCCCCCGCCGGAGGGTTGTTTCCTGGACCAATATATTAAAGAAAGCGGCTTCAAGGTTTGAGATCG
CGACAGCGAAAGGCGCCACC

Downstream 100 bases:

>100_bases
AGGCCACACAGCACCCCGCGTGGTGGTGCAAAACAGCGACATGTCAGCAGGTTAAACGGCGCGCTTCTCCAAAAGCTGCG
CCGTTTTCCGTTGCCCGAAC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFTTLTGIAAPMPLINVDTDMIIPKQFLKTIKRSGLGANLFDEMRFDDDGNEIPDFVLNKPAYRDAQILVAGDNFGCG
SSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIVLLQEQVDMLMDDAERGANAVVSVDLESQTITGPDGGTISF
EVDAFRKHCLLNGLDDIGLTLEKAPSIKAFEAEAAQARPWV

Sequences:

>Translated_201_residues
MDKFTTLTGIAAPMPLINVDTDMIIPKQFLKTIKRSGLGANLFDEMRFDDDGNEIPDFVLNKPAYRDAQILVAGDNFGCG
SSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIVLLQEQVDMLMDDAERGANAVVSVDLESQTITGPDGGTISF
EVDAFRKHCLLNGLDDIGLTLEKAPSIKAFEAEAAQARPWV
>Mature_201_residues
MDKFTTLTGIAAPMPLINVDTDMIIPKQFLKTIKRSGLGANLFDEMRFDDDGNEIPDFVLNKPAYRDAQILVAGDNFGCG
SSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIVLLQEQVDMLMDDAERGANAVVSVDLESQTITGPDGGTISF
EVDAFRKHCLLNGLDDIGLTLEKAPSIKAFEAEAAQARPWV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=51.5463917525773, Blast_Score=201, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=58.2914572864322, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_JANSC (Q28W72)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508065.1
- ProteinModelPortal:   Q28W72
- SMR:   Q28W72
- STRING:   Q28W72
- GeneID:   3932559
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0123
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q28W72
- ProtClustDB:   PRK01641
- BioCyc:   JSP290400:JANN_0123-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22017; Mature: 22017

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTTLTGIAAPMPLINVDTDMIIPKQFLKTIKRSGLGANLFDEMRFDDDGNEIPDFVL
CCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHH
NKPAYRDAQILVAGDNFGCGSSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEHHHHHHHHHHHHHHHCCCEEEE
LLQEQVDMLMDDAERGANAVVSVDLESQTITGPDGGTISFEVDAFRKHCLLNGLDDIGLT
EHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKAPSIKAFEAEAAQARPWV
EECCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MDKFTTLTGIAAPMPLINVDTDMIIPKQFLKTIKRSGLGANLFDEMRFDDDGNEIPDFVL
CCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHH
NKPAYRDAQILVAGDNFGCGSSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCEEHHHHHHHHHHHHHHHCCCEEEE
LLQEQVDMLMDDAERGANAVVSVDLESQTITGPDGGTISFEVDAFRKHCLLNGLDDIGLT
EHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKAPSIKAFEAEAAQARPWV
EECCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA