Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 89052585

GI number: 89052585

Start: 88325

End: 89191

Strand: Direct

Name: ilvE [H]

Synonym: Jann_0094

Alternate gene names: 89052585

Gene position: 88325-89191 (Clockwise)

Preceding gene: 89052578

Following gene: 89052588

Centisome position: 2.05

GC content: 61.13

Gene sequence:

>867_bases
ATGGCAGGATATGATGACAGGGACGGCATGATCTGGATGGACGGCAAGCTCGTCGACTGGCGCGCCGCCAACGTCCATCT
TTTGTCCCACGCGATGCACTACGCCTCATCGGTGTTTGAAGGGGAGCGCGCCTATAACGGCAAGATCTTCGAGAGCCGGA
AGCACTCCGAACGGCTGCATTACTCCGCCTCCTGCATCGATTTTGAGATCCCCTTCACCGTCGATGAGATTGAGGCCGCG
AAATACGAGGTCATGAAGGCCAATGGCCTGACCGACGCCTACATTCGGGCCGTTGCCTGGCGTGGCGCGGGGGAGGACAT
GGGTGTCAGCTCCGCGCGCAATCCGGTGCGCTTGGCCGTGGCTGCATGGGAATGGGGCAGCTACTATGGTGACGCGAAGA
TGAAGGGTGCGAAACTGGATATCAGCAAGTGGAAACGCCCCTCGCCCGAGACGATCCCGGTGCATGCCAAGGCGGCTGGC
CTCTACATGATCTGCACGACATCCAAGCACGCGGCGGAGGCCAAGGGCTGCTCGGACGCGCTGTTCATGGATTACCGCAA
CTACGTGGCCGAGGCGACGGGCGCCAACATCTTCTTCGTCAAGGACGGCGAGGTGCACACCCCAACCCCCGATTGCTTCC
TGAACGGCATCACGCGCCAGACGGTCGTGGGCATGCTGAAGGACAAGCAAATCAAGGTCCACGAGCGGCACATCATGCCC
GAAGAACTTGAGTCGTTCGAGCAATGCTGGCTGACCGGCACAGCGGCAGAAGTCACGCCGGTGGGCCACATCGGGGATTA
CAACTTCGAGGTCGGAGCGCTCACCCGCGACATCGCGGAAAGCTATGAGGCGTTGGTGCGGGCTTGA

Upstream 100 bases:

>100_bases
GCGTTTTGCGTAATTTTATGTCCGCATCGGACGTATTTTGCGCAACTTTTGGAAACCTACGGCCCGTGTGGAAGCGGGAT
TATGGACGAGGATCAAGACA

Downstream 100 bases:

>100_bases
ACAACAAACAAGCTTGGACCCGGCTCCGCCAACTCCGCGGGGCCGGGTCTTTTTAGACGCATGTCCGTTTCTCTAGTCGG
GCTTGGGGTCAGGCTCGGTT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAGYDDRDGMIWMDGKLVDWRAANVHLLSHAMHYASSVFEGERAYNGKIFESRKHSERLHYSASCIDFEIPFTVDEIEAA
KYEVMKANGLTDAYIRAVAWRGAGEDMGVSSARNPVRLAVAAWEWGSYYGDAKMKGAKLDISKWKRPSPETIPVHAKAAG
LYMICTTSKHAAEAKGCSDALFMDYRNYVAEATGANIFFVKDGEVHTPTPDCFLNGITRQTVVGMLKDKQIKVHERHIMP
EELESFEQCWLTGTAAEVTPVGHIGDYNFEVGALTRDIAESYEALVRA

Sequences:

>Translated_288_residues
MAGYDDRDGMIWMDGKLVDWRAANVHLLSHAMHYASSVFEGERAYNGKIFESRKHSERLHYSASCIDFEIPFTVDEIEAA
KYEVMKANGLTDAYIRAVAWRGAGEDMGVSSARNPVRLAVAAWEWGSYYGDAKMKGAKLDISKWKRPSPETIPVHAKAAG
LYMICTTSKHAAEAKGCSDALFMDYRNYVAEATGANIFFVKDGEVHTPTPDCFLNGITRQTVVGMLKDKQIKVHERHIMP
EELESFEQCWLTGTAAEVTPVGHIGDYNFEVGALTRDIAESYEALVRA
>Mature_287_residues
AGYDDRDGMIWMDGKLVDWRAANVHLLSHAMHYASSVFEGERAYNGKIFESRKHSERLHYSASCIDFEIPFTVDEIEAAK
YEVMKANGLTDAYIRAVAWRGAGEDMGVSSARNPVRLAVAAWEWGSYYGDAKMKGAKLDISKWKRPSPETIPVHAKAAGL
YMICTTSKHAAEAKGCSDALFMDYRNYVAEATGANIFFVKDGEVHTPTPDCFLNGITRQTVVGMLKDKQIKVHERHIMPE
ELESFEQCWLTGTAAEVTPVGHIGDYNFEVGALTRDIAESYEALVRA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=285, Percent_Identity=35.7894736842105, Blast_Score=174, Evalue=5e-45,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32117; Mature: 31985

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGYDDRDGMIWMDGKLVDWRAANVHLLSHAMHYASSVFEGERAYNGKIFESRKHSERLH
CCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCHHCCCCEECCCCHHHHHE
YSASCIDFEIPFTVDEIEAAKYEVMKANGLTDAYIRAVAWRGAGEDMGVSSARNPVRLAV
EEEEEEEEECCCCHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
AAWEWGSYYGDAKMKGAKLDISKWKRPSPETIPVHAKAAGLYMICTTSKHAAEAKGCSDA
EEEECCCEECCCCCCCCEEEHHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHCCCCHH
LFMDYRNYVAEATGANIFFVKDGEVHTPTPDCFLNGITRQTVVGMLKDKQIKVHERHIMP
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEHHHCCCH
EELESFEQCWLTGTAAEVTPVGHIGDYNFEVGALTRDIAESYEALVRA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGYDDRDGMIWMDGKLVDWRAANVHLLSHAMHYASSVFEGERAYNGKIFESRKHSERLH
CCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHHHHCCCHHCCCCEECCCCHHHHHE
YSASCIDFEIPFTVDEIEAAKYEVMKANGLTDAYIRAVAWRGAGEDMGVSSARNPVRLAV
EEEEEEEEECCCCHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
AAWEWGSYYGDAKMKGAKLDISKWKRPSPETIPVHAKAAGLYMICTTSKHAAEAKGCSDA
EEEECCCEECCCCCCCCEEEHHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHCCCCHH
LFMDYRNYVAEATGANIFFVKDGEVHTPTPDCFLNGITRQTVVGMLKDKQIKVHERHIMP
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEHHHCCCH
EELESFEQCWLTGTAAEVTPVGHIGDYNFEVGALTRDIAESYEALVRA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]