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The map label for this gene is folC [H]

Identifier: 89052550

GI number: 89052550

Start: 53649

End: 54914

Strand: Reverse

Name: folC [H]

Synonym: Jann_0059

Alternate gene names: 89052550

Gene position: 54914-53649 (Counterclockwise)

Preceding gene: 89052551

Following gene: 89052549

Centisome position: 1.27

GC content: 62.48

Gene sequence:

>1266_bases
ATGACGTCCCATTCGGACCAATTGCTCGCGCGCATGATGGCGCTCCACCCCAAGATCATCGATCTGACGCTGGATCGCAT
GTGGCGCATTCTGTACGCTTTGGGTGATCCTCAAACGCGCATTCCACCGGTGATCCACGTCGCGGGCACAAACGGGAAAG
GGTCGACCCAGGCCATGATCCGCGCGGGGCTGGAAGGCGCGGGGCTGACCACGCACGCCTATACGTCGCCGCATCTTGCA
CGATTTCATGAGCGTATCCGCCTTGCCGGAGAGTTGATTGATGAAGATCACCTGTCCCGCATTCTCGACACCTGCCTCGC
GGCCAATGGCCCCGATCCGATCACGTATTTTGAGATCACCACCGCAGCTGCTTTGCACGCCTTCGCACAAATGCCTGCCG
ACTACACCTTGCTTGAGGTCGGCCTTGGCGGCCGTCTGGACGCGACCAATGTGATTGACGCGCCAGCGCTGAGCGTCATC
ACCCCCATCGACCTCGATCACCAGCAGTTTCTGGGTGATACGCTGGAGGCCATCGCTGGAGAGAAAGCCGGGATCATCAA
ACGCGGCATTCCCGTCGTCGTTGGCCCCCAGCAGGACGAAGCGCTTGAGGTGATCGAGGCGCGTGCCGCCCGGCTGGGTG
CGCCCGTTATCGCCCATGGGCAGCACTGGCATGTCACGACCGAGCGGGAGCGGCTGATTTTCCAGGATGAAACGGGCCTG
CTTGACCTGCCGCTGCCCTCCCTCGCGGGGCCCCATCAGATCCAGAATGCGGGTACAGCGCTTGCCGCCTTACGCACCTT
GGGTGTGGGTGACGCGGATGCCGCGATGACCGACACCATCTGGCCTGCACGGATGCAAAAACTGCGCCACGGCCCCCTGA
CGGAGGTAGCGCCGAGTGTCGAGCTTTGGCTGGACGGCGGCCATAACCCCCACGCCGCCCGCGCCATTGCGGCGACGCTG
GAAACGCTTCCCAAGCGACCGACCTACCTGATCTGTGGTATGCTCAACACCAAGGATGTGACCGGTTATATGGCACCTTT
GGTCCCTTGGGTTGAAAGCCTCACCGCTGTGTCCATTCCCGGTCAAACCGCGACGCTCAGCGCCGAAGAGACGGCCGAAG
CGGCCCGATCGGTAGGCCTCACGACCCGTATCGCACCCGGCGTTGACGCTGCCCTGACTGAAATCGCGGTGGACGATCCC
AGGGCCCGCGTGCTGATTTGCGGCTCCCTCTATCTTGCCGGAGAGATCTTGAAAACGAACGGTTGA

Upstream 100 bases:

>100_bases
TCCAAGGACTAGCGCTCCGCTCCGTCTTCATCTTGGCCATACAACTCCCGCCGGAGGCTTCCATGGTCTGTCGCCCGGCG
CTTCGTTTGCAGGATATGCC

Downstream 100 bases:

>100_bases
TGGCGCGCGCAGCCTGCCCTTTCCGGGCGCGCGCTCCGTGGTCAATTGGCCGGCGTGTCATTGGTTGACGTCTCGTGCCG
CAAGCCCCACTTTCTGCCCA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTSHSDQLLARMMALHPKIIDLTLDRMWRILYALGDPQTRIPPVIHVAGTNGKGSTQAMIRAGLEGAGLTTHAYTSPHLA
RFHERIRLAGELIDEDHLSRILDTCLAANGPDPITYFEITTAAALHAFAQMPADYTLLEVGLGGRLDATNVIDAPALSVI
TPIDLDHQQFLGDTLEAIAGEKAGIIKRGIPVVVGPQQDEALEVIEARAARLGAPVIAHGQHWHVTTERERLIFQDETGL
LDLPLPSLAGPHQIQNAGTALAALRTLGVGDADAAMTDTIWPARMQKLRHGPLTEVAPSVELWLDGGHNPHAARAIAATL
ETLPKRPTYLICGMLNTKDVTGYMAPLVPWVESLTAVSIPGQTATLSAEETAEAARSVGLTTRIAPGVDAALTEIAVDDP
RARVLICGSLYLAGEILKTNG

Sequences:

>Translated_421_residues
MTSHSDQLLARMMALHPKIIDLTLDRMWRILYALGDPQTRIPPVIHVAGTNGKGSTQAMIRAGLEGAGLTTHAYTSPHLA
RFHERIRLAGELIDEDHLSRILDTCLAANGPDPITYFEITTAAALHAFAQMPADYTLLEVGLGGRLDATNVIDAPALSVI
TPIDLDHQQFLGDTLEAIAGEKAGIIKRGIPVVVGPQQDEALEVIEARAARLGAPVIAHGQHWHVTTERERLIFQDETGL
LDLPLPSLAGPHQIQNAGTALAALRTLGVGDADAAMTDTIWPARMQKLRHGPLTEVAPSVELWLDGGHNPHAARAIAATL
ETLPKRPTYLICGMLNTKDVTGYMAPLVPWVESLTAVSIPGQTATLSAEETAEAARSVGLTTRIAPGVDAALTEIAVDDP
RARVLICGSLYLAGEILKTNG
>Mature_420_residues
TSHSDQLLARMMALHPKIIDLTLDRMWRILYALGDPQTRIPPVIHVAGTNGKGSTQAMIRAGLEGAGLTTHAYTSPHLAR
FHERIRLAGELIDEDHLSRILDTCLAANGPDPITYFEITTAAALHAFAQMPADYTLLEVGLGGRLDATNVIDAPALSVIT
PIDLDHQQFLGDTLEAIAGEKAGIIKRGIPVVVGPQQDEALEVIEARAARLGAPVIAHGQHWHVTTERERLIFQDETGLL
DLPLPSLAGPHQIQNAGTALAALRTLGVGDADAAMTDTIWPARMQKLRHGPLTEVAPSVELWLDGGHNPHAARAIAATLE
TLPKRPTYLICGMLNTKDVTGYMAPLVPWVESLTAVSIPGQTATLSAEETAEAARSVGLTTRIAPGVDAALTEIAVDDPR
ARVLICGSLYLAGEILKTNG

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=314, Percent_Identity=31.8471337579618, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI66932990, Length=314, Percent_Identity=31.8471337579618, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=35.5769230769231, Blast_Score=185, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17553148, Length=299, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=299, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=299, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=425, Percent_Identity=29.1764705882353, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=446, Percent_Identity=25.5605381165919, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322718, Length=436, Percent_Identity=24.0825688073394, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=39.1061452513966, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24581568, Length=175, Percent_Identity=36.5714285714286, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44931; Mature: 44800

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHSDQLLARMMALHPKIIDLTLDRMWRILYALGDPQTRIPPVIHVAGTNGKGSTQAMI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHH
RAGLEGAGLTTHAYTSPHLARFHERIRLAGELIDEDHLSRILDTCLAANGPDPITYFEIT
HHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHH
TAAALHAFAQMPADYTLLEVGLGGRLDATNVIDAPALSVITPIDLDHQQFLGDTLEAIAG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHCC
EKAGIIKRGIPVVVGPQQDEALEVIEARAARLGAPVIAHGQHWHVTTERERLIFQDETGL
CCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEECCCCE
LDLPLPSLAGPHQIQNAGTALAALRTLGVGDADAAMTDTIWPARMQKLRHGPLTEVAPSV
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHCCCE
ELWLDGGHNPHAARAIAATLETLPKRPTYLICGMLNTKDVTGYMAPLVPWVESLTAVSIP
EEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEECC
GQTATLSAEETAEAARSVGLTTRIAPGVDAALTEIAVDDPRARVLICGSLYLAGEILKTN
CCCEEECHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCCCEEEEEECHHHEEEEEECC
G
C
>Mature Secondary Structure 
TSHSDQLLARMMALHPKIIDLTLDRMWRILYALGDPQTRIPPVIHVAGTNGKGSTQAMI
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHH
RAGLEGAGLTTHAYTSPHLARFHERIRLAGELIDEDHLSRILDTCLAANGPDPITYFEIT
HHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEHH
TAAALHAFAQMPADYTLLEVGLGGRLDATNVIDAPALSVITPIDLDHQQFLGDTLEAIAG
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHCC
EKAGIIKRGIPVVVGPQQDEALEVIEARAARLGAPVIAHGQHWHVTTERERLIFQDETGL
CCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEECCCCE
LDLPLPSLAGPHQIQNAGTALAALRTLGVGDADAAMTDTIWPARMQKLRHGPLTEVAPSV
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHCCCE
ELWLDGGHNPHAARAIAATLETLPKRPTYLICGMLNTKDVTGYMAPLVPWVESLTAVSIP
EEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEECC
GQTATLSAEETAEAARSVGLTTRIAPGVDAALTEIAVDDPRARVLICGSLYLAGEILKTN
CCCEEECHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCCCCCEEEEEECHHHEEEEEECC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]