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The map label for this gene is slt [H]

Identifier: 89052525

GI number: 89052525

Start: 31209

End: 33158

Strand: Direct

Name: slt [H]

Synonym: Jann_0034

Alternate gene names: 89052525

Gene position: 31209-33158 (Clockwise)

Preceding gene: 89052513

Following gene: 89052526

Centisome position: 0.72

GC content: 63.9

Gene sequence:

>1950_bases
ATGGGTTTGCGACGCCTGATCACGTCTATTGTCCTCTGCTCGCTCCTCGGGACGCCTGCCGCCACCGCCCAATCGGATGC
AGACGCCCTGGCCGCAGCGCTTGCCCGCTACAATGTGGGCGATCTTGCCGGTGGCTCGAATGCCGCGCGGGATCTCACTG
ACCCGGTGGCGCTGGATATCCTCGCCTGGACCCGGCTGCGGCGTGGGGAGGGCAGTTTTGAGGAATATATCTCCTTCCTC
GACCGCAACCCGGACTGGCCGGGTCTGGCGTATCTGCGCCGCCAGGGGGAGCCGTCTATTCCAGAGGGGGGCGATGCGGC
CTCAATCCTGGATTACTTCGCTGATGTCCTGCCCCAGACGGGCGCTGGATCCCTGGCGCTGGCCCGCGCTTTGGCCGATA
CCGGAAACCCCGTGGTCGCCGAGGCCATTGTCGTGCGGGCCTGGACATCTCTGGTGATGACGGGCACCGACGCCCAGGCC
CTGAACCGCGCCTTCGGGAACCTGCTGACGCAGGACCACCACATTGCCCGGCTTGACCACCTGCTGTGGGAAAACGCGGA
GGATCGCGCGCGCGCCATGTTCTCCCTCGTGCCCGAAGACTACGTGCCTTTGGCCGAAGCGCGTCTGGCCCTGCGAGATC
GCAGACCGGGGGTGAACGGCCTGATTGATGCGATCCCCGCGTCATTGCAGGATGATCCCGGCCTCGCCTATGAACGCTTC
ATCTGGCGGGTGCGCGAAGGGCTTTGGGACACGGCTGGTGCGCTGATGGTCGAACGCTCCACCTCTCCTGACACTCTGGG
ACGACCGGCGGAATGGGGTCGCCGTCGCGCCGATCTGGCCCGCGACGTAATGCGCGAAGGCGATCACGACACCTGTTATG
ATTACGCATCCAACCATCAGATCGATCCCGAGGACGACTACATCCGTTTTGCCGATCTGGAATGGGTTGCGGGCTATTGC
GCCTTGCAACTGAACCGGGCTGACATCGCCGCCAATCACTTCCAGCGCTTCAGTGAGGTCGTCTTCTCGCCCATCTCTGT
CGGGCGCGCGGGCTATTGGCTTGGCCGTGCCCATGAGGCCGCAGGCAATGCCGCGGCGGCGGCAGACGGCTACGCTCTGG
GCGCGCAATATCAGTCCAGTTTCTACGGCCAACTGGCACAGGAACGCGGCGGGTTGCCTGTCGATCCGGAGTTCACGGGC
ACCCGCGACTATGGCGATTGGCGCAATTCCAGCTTCGCGGATCTCGATGTGTTTCGTGCGGCGATCCTGCTTTACGAGGC
GGGCGAGGTGAACCTGGCAGAACGGTTCTTCACCCATATCACGGAACGCCTGGATGAGACGGAGGCCGGTATGATGGGCG
CGTTGATGCTGGAGTTGGAAGAGCCGCACTACGCGCTGATGGTCGCCAAACGCGCCGCCCAGTCGGGGCATGAAATCTAC
CCCGCCTACTACCCGCTGGCCGATTTGGAAGATGTGGACCTGCCCGTCCCCCGCGCCTTTGCCCTATCCATCGCGCGGCG
CGAAAGTGAATTTGACCCCGTCGTCGTGTCAGGCGCGGGCGCGATTGGGCTGATGCAGGTGATGCCCGGCACGGGACGCG
ACTCCGCCGGTGAGCTGGGGCTCGACTTTTCCGAGGCCCGCTTGCGCAATGACCCCGCCTATAACGTGCTTCTGGGCTCC
AACTATATCGCCGGGCTGCTGGAGGATTATGACGGCAATCCGGTGCTGGTCACGATCGGCTATAACGCCGGGCCGGGCCG
CGTGCGGCAATGGCGGGAGCGGTTTGGCGATCCCCGTCAGGCCGACGACGTGATCGACTGGATCGAAGACGTGCCATTCT
CGGAGACACGCAATTACATCATGCGAGTGATCGAAAGCCTGCCCATCTATGAGGCGCAACTGACCGGCGAATTGCCGACC
TTGGGATTGAGCGAGCGCTTGGTGCAATAG

Upstream 100 bases:

>100_bases
AATACAAGGCTCGTGCAGAATTGCCAAGTTCGTGAGTTGCTTCCTTGGGTTTGAGGGGTATTTTGTGGGCTCTGGGGATA
ACTTATTATGGAGACGCCGC

Downstream 100 bases:

>100_bases
GCCGGATTGGCATCATACTGCAAACGGTGCTGGACCAGGCGCGTGAATAGACTAGGGTCGGGTCATGGACTGGACAGTTT
TCATCTTGTTTTTCGGGGCT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MGLRRLITSIVLCSLLGTPAATAQSDADALAAALARYNVGDLAGGSNAARDLTDPVALDILAWTRLRRGEGSFEEYISFL
DRNPDWPGLAYLRRQGEPSIPEGGDAASILDYFADVLPQTGAGSLALARALADTGNPVVAEAIVVRAWTSLVMTGTDAQA
LNRAFGNLLTQDHHIARLDHLLWENAEDRARAMFSLVPEDYVPLAEARLALRDRRPGVNGLIDAIPASLQDDPGLAYERF
IWRVREGLWDTAGALMVERSTSPDTLGRPAEWGRRRADLARDVMREGDHDTCYDYASNHQIDPEDDYIRFADLEWVAGYC
ALQLNRADIAANHFQRFSEVVFSPISVGRAGYWLGRAHEAAGNAAAAADGYALGAQYQSSFYGQLAQERGGLPVDPEFTG
TRDYGDWRNSSFADLDVFRAAILLYEAGEVNLAERFFTHITERLDETEAGMMGALMLELEEPHYALMVAKRAAQSGHEIY
PAYYPLADLEDVDLPVPRAFALSIARRESEFDPVVVSGAGAIGLMQVMPGTGRDSAGELGLDFSEARLRNDPAYNVLLGS
NYIAGLLEDYDGNPVLVTIGYNAGPGRVRQWRERFGDPRQADDVIDWIEDVPFSETRNYIMRVIESLPIYEAQLTGELPT
LGLSERLVQ

Sequences:

>Translated_649_residues
MGLRRLITSIVLCSLLGTPAATAQSDADALAAALARYNVGDLAGGSNAARDLTDPVALDILAWTRLRRGEGSFEEYISFL
DRNPDWPGLAYLRRQGEPSIPEGGDAASILDYFADVLPQTGAGSLALARALADTGNPVVAEAIVVRAWTSLVMTGTDAQA
LNRAFGNLLTQDHHIARLDHLLWENAEDRARAMFSLVPEDYVPLAEARLALRDRRPGVNGLIDAIPASLQDDPGLAYERF
IWRVREGLWDTAGALMVERSTSPDTLGRPAEWGRRRADLARDVMREGDHDTCYDYASNHQIDPEDDYIRFADLEWVAGYC
ALQLNRADIAANHFQRFSEVVFSPISVGRAGYWLGRAHEAAGNAAAAADGYALGAQYQSSFYGQLAQERGGLPVDPEFTG
TRDYGDWRNSSFADLDVFRAAILLYEAGEVNLAERFFTHITERLDETEAGMMGALMLELEEPHYALMVAKRAAQSGHEIY
PAYYPLADLEDVDLPVPRAFALSIARRESEFDPVVVSGAGAIGLMQVMPGTGRDSAGELGLDFSEARLRNDPAYNVLLGS
NYIAGLLEDYDGNPVLVTIGYNAGPGRVRQWRERFGDPRQADDVIDWIEDVPFSETRNYIMRVIESLPIYEAQLTGELPT
LGLSERLVQ
>Mature_648_residues
GLRRLITSIVLCSLLGTPAATAQSDADALAAALARYNVGDLAGGSNAARDLTDPVALDILAWTRLRRGEGSFEEYISFLD
RNPDWPGLAYLRRQGEPSIPEGGDAASILDYFADVLPQTGAGSLALARALADTGNPVVAEAIVVRAWTSLVMTGTDAQAL
NRAFGNLLTQDHHIARLDHLLWENAEDRARAMFSLVPEDYVPLAEARLALRDRRPGVNGLIDAIPASLQDDPGLAYERFI
WRVREGLWDTAGALMVERSTSPDTLGRPAEWGRRRADLARDVMREGDHDTCYDYASNHQIDPEDDYIRFADLEWVAGYCA
LQLNRADIAANHFQRFSEVVFSPISVGRAGYWLGRAHEAAGNAAAAADGYALGAQYQSSFYGQLAQERGGLPVDPEFTGT
RDYGDWRNSSFADLDVFRAAILLYEAGEVNLAERFFTHITERLDETEAGMMGALMLELEEPHYALMVAKRAAQSGHEIYP
AYYPLADLEDVDLPVPRAFALSIARRESEFDPVVVSGAGAIGLMQVMPGTGRDSAGELGLDFSEARLRNDPAYNVLLGSN
YIAGLLEDYDGNPVLVTIGYNAGPGRVRQWRERFGDPRQADDVIDWIEDVPFSETRNYIMRVIESLPIYEAQLTGELPTL
GLSERLVQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=147, Percent_Identity=36.0544217687075, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 71281; Mature: 71150

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRRLITSIVLCSLLGTPAATAQSDADALAAALARYNVGDLAGGSNAARDLTDPVALDI
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHH
LAWTRLRRGEGSFEEYISFLDRNPDWPGLAYLRRQGEPSIPEGGDAASILDYFADVLPQT
HHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
GAGSLALARALADTGNPVVAEAIVVRAWTSLVMTGTDAQALNRAFGNLLTQDHHIARLDH
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
LLWENAEDRARAMFSLVPEDYVPLAEARLALRDRRPGVNGLIDAIPASLQDDPGLAYERF
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHH
IWRVREGLWDTAGALMVERSTSPDTLGRPAEWGRRRADLARDVMREGDHDTCYDYASNHQ
HHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
IDPEDDYIRFADLEWVAGYCALQLNRADIAANHFQRFSEVVFSPISVGRAGYWLGRAHEA
CCCCCCCEEEECHHHHHHHHHEEECHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHH
AGNAAAAADGYALGAQYQSSFYGQLAQERGGLPVDPEFTGTRDYGDWRNSSFADLDVFRA
CCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AILLYEAGEVNLAERFFTHITERLDETEAGMMGALMLELEEPHYALMVAKRAAQSGHEIY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCC
PAYYPLADLEDVDLPVPRAFALSIARRESEFDPVVVSGAGAIGLMQVMPGTGRDSAGELG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCC
LDFSEARLRNDPAYNVLLGSNYIAGLLEDYDGNPVLVTIGYNAGPGRVRQWRERFGDPRQ
CCHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
ADDVIDWIEDVPFSETRNYIMRVIESLPIYEAQLTGELPTLGLSERLVQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHCCCCCCCCHHHHHCC
>Mature Secondary Structure 
GLRRLITSIVLCSLLGTPAATAQSDADALAAALARYNVGDLAGGSNAARDLTDPVALDI
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHH
LAWTRLRRGEGSFEEYISFLDRNPDWPGLAYLRRQGEPSIPEGGDAASILDYFADVLPQT
HHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
GAGSLALARALADTGNPVVAEAIVVRAWTSLVMTGTDAQALNRAFGNLLTQDHHIARLDH
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
LLWENAEDRARAMFSLVPEDYVPLAEARLALRDRRPGVNGLIDAIPASLQDDPGLAYERF
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHH
IWRVREGLWDTAGALMVERSTSPDTLGRPAEWGRRRADLARDVMREGDHDTCYDYASNHQ
HHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
IDPEDDYIRFADLEWVAGYCALQLNRADIAANHFQRFSEVVFSPISVGRAGYWLGRAHEA
CCCCCCCEEEECHHHHHHHHHEEECHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHH
AGNAAAAADGYALGAQYQSSFYGQLAQERGGLPVDPEFTGTRDYGDWRNSSFADLDVFRA
CCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AILLYEAGEVNLAERFFTHITERLDETEAGMMGALMLELEEPHYALMVAKRAAQSGHEIY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCC
PAYYPLADLEDVDLPVPRAFALSIARRESEFDPVVVSGAGAIGLMQVMPGTGRDSAGELG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCC
LDFSEARLRNDPAYNVLLGSNYIAGLLEDYDGNPVLVTIGYNAGPGRVRQWRERFGDPRQ
CCHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
ADDVIDWIEDVPFSETRNYIMRVIESLPIYEAQLTGELPTLGLSERLVQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]