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The map label for this gene is ubiB [H]

Identifier: 88658640

GI number: 88658640

Start: 858211

End: 859653

Strand: Reverse

Name: ubiB [H]

Synonym: ECH_0840

Alternate gene names: 88658640

Gene position: 859653-858211 (Counterclockwise)

Preceding gene: 88658222

Following gene: 88658624

Centisome position: 73.08

GC content: 30.63

Gene sequence:

>1443_bases
ATGCTTAGTACCTTCTCTAATATAATTTCTTTACTGAATATATTAAGTGTTTTAGTAAGGTATAACATATTACCTTATAC
CTATCTTTTTGCTAAAAGATCAATTAGGAAAAAATCTCGAGGAGAGAAGATTGCAATGGCTATGCAAAAACTTGGCCCAA
TTTTTATTAAATTTGGTCAATCTATATCTTCTAGGACTGATATTATTGGGGAAGAAATTGCAAGTCATCTGCTCTGTTTA
TGTGATAAATTACCAGCTTTTCCTTATGTAGATGTTGTAAAAATTGTAGAGGAGGATTTTAAGTGTAAGATAAATGAAGT
GTTTTGTGAATTTGAGCAGAACCCTATTGCTGCTGCATCTATTGCACAGGTACATAAGGCTAAAGATATTAATGGCAATG
TAAGGGCTGTGAAGGTTTTGAGACCACGCATTGAAAAAATTTTTGAACGGGATATTAAATTAATGTTTTGGCTTGCAAGG
GTAGCACAAAACTTTCAAAGTCTGCAACGATTTCAATTATTACAGCTTGTACAAACATTTCATGAGATATGTAAAATGGA
AATAGATTTGCGTTTTGAAGCAGCAAATGCTGAGGAGTTGAGATCTAATATTCAGAGTGATAAGGGATGTTATGTACCAG
AAGTAGATTGGACTAGGACTACAAGACGTGTATTGACATTAGAATGGATAGATGCTGTACCAATATATAATGTTTACAAG
TCAGAGAATTGTAAAGCACTAGTGGAAAATTTAATCTTTTGTTTTTGTAATCAAGTGTATAGAGATAGATTTTTTCATGC
AGATATGCATCCAGGAAATCTTATGGTAGATGCAAAGGGAAGAGTTGTAATTATAGATTTTGGAATTGTGGGGAGGTTAG
ATAAAGCTACATGTATGTATGTTACAGAGATACTTGTAGGTTTCTTAAAAAGAGATTATCAATATGTTGCTGATATCCAT
TATAGAGCTGGATATGTTGATGCAAAATATAGTAATTTTGTTACAGCTTGTTGTGCTATAGGTGAACCTATAGTTGGTAA
AAATACGGAAAATATATCTATTTCAGCATTATTAACTCAATTATTTAAAGTTACTGCTGATTTTAATATGAAATTGCAAC
CCCAACTTTTTCTATTACAAAAAACTACAGTATTGATAGAAGGTGTATGTAGGCAATTGTGTCCTGAAGTTAATATGTGG
AAAATAGCGGAATTATGGATGGAAAAGTATGCTATTGAAAAAAATAAATATATTCAAAAATTAAAAAGATCTTACTTGTG
TCAAGCTATACATGATGTTCCTTTATTTATGAAAAAAGTTGATAAAAGTTTAGATTTAATAAATAACTATCAAGAGCTAA
GTAAAGCAAAAACAAGTATTAGTAGAGATGTTATATGGGCATTAATAGTACTTGGTTTAATTATCAAGCTAATTATAGGA
TAG

Upstream 100 bases:

>100_bases
AATTCTTATCGTGTTATATGTATTAATAGGAGTATTTATTGTGAAGGATCTCCAAGTGAGATTGTAAAAAATGAAAAGTT
CTTAAAAATGTTTTCATCTT

Downstream 100 bases:

>100_bases
TTGTTGTTATTAGGTATAAATTAATATAGGGTTTATAGATCTAAAATGATTATAAATTGTATGTTCAATAATATAAATTG
ATTAAGTATTTTGTATCAGG

Product: 2-polyprenylphenol 6-hydroxylase

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLSTFSNIISLLNILSVLVRYNILPYTYLFAKRSIRKKSRGEKIAMAMQKLGPIFIKFGQSISSRTDIIGEEIASHLLCL
CDKLPAFPYVDVVKIVEEDFKCKINEVFCEFEQNPIAAASIAQVHKAKDINGNVRAVKVLRPRIEKIFERDIKLMFWLAR
VAQNFQSLQRFQLLQLVQTFHEICKMEIDLRFEAANAEELRSNIQSDKGCYVPEVDWTRTTRRVLTLEWIDAVPIYNVYK
SENCKALVENLIFCFCNQVYRDRFFHADMHPGNLMVDAKGRVVIIDFGIVGRLDKATCMYVTEILVGFLKRDYQYVADIH
YRAGYVDAKYSNFVTACCAIGEPIVGKNTENISISALLTQLFKVTADFNMKLQPQLFLLQKTTVLIEGVCRQLCPEVNMW
KIAELWMEKYAIEKNKYIQKLKRSYLCQAIHDVPLFMKKVDKSLDLINNYQELSKAKTSISRDVIWALIVLGLIIKLIIG

Sequences:

>Translated_480_residues
MLSTFSNIISLLNILSVLVRYNILPYTYLFAKRSIRKKSRGEKIAMAMQKLGPIFIKFGQSISSRTDIIGEEIASHLLCL
CDKLPAFPYVDVVKIVEEDFKCKINEVFCEFEQNPIAAASIAQVHKAKDINGNVRAVKVLRPRIEKIFERDIKLMFWLAR
VAQNFQSLQRFQLLQLVQTFHEICKMEIDLRFEAANAEELRSNIQSDKGCYVPEVDWTRTTRRVLTLEWIDAVPIYNVYK
SENCKALVENLIFCFCNQVYRDRFFHADMHPGNLMVDAKGRVVIIDFGIVGRLDKATCMYVTEILVGFLKRDYQYVADIH
YRAGYVDAKYSNFVTACCAIGEPIVGKNTENISISALLTQLFKVTADFNMKLQPQLFLLQKTTVLIEGVCRQLCPEVNMW
KIAELWMEKYAIEKNKYIQKLKRSYLCQAIHDVPLFMKKVDKSLDLINNYQELSKAKTSISRDVIWALIVLGLIIKLIIG
>Mature_480_residues
MLSTFSNIISLLNILSVLVRYNILPYTYLFAKRSIRKKSRGEKIAMAMQKLGPIFIKFGQSISSRTDIIGEEIASHLLCL
CDKLPAFPYVDVVKIVEEDFKCKINEVFCEFEQNPIAAASIAQVHKAKDINGNVRAVKVLRPRIEKIFERDIKLMFWLAR
VAQNFQSLQRFQLLQLVQTFHEICKMEIDLRFEAANAEELRSNIQSDKGCYVPEVDWTRTTRRVLTLEWIDAVPIYNVYK
SENCKALVENLIFCFCNQVYRDRFFHADMHPGNLMVDAKGRVVIIDFGIVGRLDKATCMYVTEILVGFLKRDYQYVADIH
YRAGYVDAKYSNFVTACCAIGEPIVGKNTENISISALLTQLFKVTADFNMKLQPQLFLLQKTTVLIEGVCRQLCPEVNMW
KIAELWMEKYAIEKNKYIQKLKRSYLCQAIHDVPLFMKKVDKSLDLINNYQELSKAKTSISRDVIWALIVLGLIIKLIIG

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254938, Length=352, Percent_Identity=25.2840909090909, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI41393593, Length=269, Percent_Identity=28.996282527881, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI217035081, Length=338, Percent_Identity=24.2603550295858, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI32261307, Length=333, Percent_Identity=25.8258258258258, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI27363457, Length=272, Percent_Identity=24.6323529411765, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI217416386, Length=272, Percent_Identity=24.6323529411765, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367309, Length=445, Percent_Identity=35.2808988764045, Blast_Score=249, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17559152, Length=367, Percent_Identity=26.7029972752044, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325148, Length=432, Percent_Identity=23.3796296296296, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323282, Length=246, Percent_Identity=26.4227642276423, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321319, Length=314, Percent_Identity=20.7006369426752, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24662575, Length=247, Percent_Identity=30.3643724696356, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI22024280, Length=398, Percent_Identity=25.6281407035176, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859849, Length=370, Percent_Identity=24.0540540540541, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 55358; Mature: 55358

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTFSNIISLLNILSVLVRYNILPYTYLFAKRSIRKKSRGEKIAMAMQKLGPIFIKFGQ
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCC
SISSRTDIIGEEIASHLLCLCDKLPAFPYVDVVKIVEEDFKCKINEVFCEFEQNPIAAAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IAQVHKAKDINGNVRAVKVLRPRIEKIFERDIKLMFWLARVAQNFQSLQRFQLLQLVQTF
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEICKMEIDLRFEAANAEELRSNIQSDKGCYVPEVDWTRTTRRVLTLEWIDAVPIYNVYK
HHHHCCHHEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEHHHCCCCHHHHHC
SENCKALVENLIFCFCNQVYRDRFFHADMHPGNLMVDAKGRVVIIDFGIVGRLDKATCMY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHH
VTEILVGFLKRDYQYVADIHYRAGYVDAKYSNFVTACCAIGEPIVGKNTENISISALLTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LFKVTADFNMKLQPQLFLLQKTTVLIEGVCRQLCPEVNMWKIAELWMEKYAIEKNKYIQK
HHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKRSYLCQAIHDVPLFMKKVDKSLDLINNYQELSKAKTSISRDVIWALIVLGLIIKLIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSTFSNIISLLNILSVLVRYNILPYTYLFAKRSIRKKSRGEKIAMAMQKLGPIFIKFGQ
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHCC
SISSRTDIIGEEIASHLLCLCDKLPAFPYVDVVKIVEEDFKCKINEVFCEFEQNPIAAAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IAQVHKAKDINGNVRAVKVLRPRIEKIFERDIKLMFWLARVAQNFQSLQRFQLLQLVQTF
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEICKMEIDLRFEAANAEELRSNIQSDKGCYVPEVDWTRTTRRVLTLEWIDAVPIYNVYK
HHHHCCHHEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEHHHCCCCHHHHHC
SENCKALVENLIFCFCNQVYRDRFFHADMHPGNLMVDAKGRVVIIDFGIVGRLDKATCMY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHH
VTEILVGFLKRDYQYVADIHYRAGYVDAKYSNFVTACCAIGEPIVGKNTENISISALLTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LFKVTADFNMKLQPQLFLLQKTTVLIEGVCRQLCPEVNMWKIAELWMEKYAIEKNKYIQK
HHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LKRSYLCQAIHDVPLFMKKVDKSLDLINNYQELSKAKTSISRDVIWALIVLGLIIKLIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA