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The map label for this gene is def [H]

Identifier: 88658603

GI number: 88658603

Start: 65137

End: 65703

Strand: Direct

Name: def [H]

Synonym: ECH_0073

Alternate gene names: 88658603

Gene position: 65137-65703 (Clockwise)

Preceding gene: 88657726

Following gene: 88657868

Centisome position: 5.54

GC content: 30.69

Gene sequence:

>567_bases
ATGTCAGTTTTGTCAATTGTTACTGTTCCTGATAAAAGATTGTCGTTATGTTCTGAAGAAGTTGAAAAAGTAGATCAATC
TATAAGAAAACTAGTTGATGATATGTTTGAAACAATGCATGCTAATCAAGGGTTAGGATTGGCTGCTGTTCAGGTTGGTG
TTCATAAACGTATTCTTGTTATGAATGTTCCAGAAGAGTTTGAAGATTCTGAGGATATTGAGAATGTTGAGGATAAAATA
GAAGGTTATGAACTATATGGAGGTCCTTATTGTATAATTAATCCTAAAATTGTTGATATATCTCAAGAGAAAGTAAAATT
AAAAGAAGGCTGCTTATCTGTTCCTGGTTATTTTGATTATATAGTACGTCCTCAGCGTATTGCTGTTCAATATTTAGATT
ATAATGGTAATGAATGTATAATAAAAGCTCAAGGGTGGCTTGCTAGATGTTTGCAACATGAGATTGACCATTTAAATGGA
ACGGTATTTTTAAAATATCTATCTAAATTTAAACGTGATTTTGCAATAGAAAAAGTGAAAAAGAAAGAACGAACTGATCT
TATATAA

Upstream 100 bases:

>100_bases
AAGTACTGATGCGCTATCAGCAACATTGTAAATCTTGAATTAATATTTGTTTCATATAATATGATTGGTAGGTTTTATAC
AAACAGTTGTAAGGAAATAA

Downstream 100 bases:

>100_bases
TTGTTGGTATTGATGTGAGTGACTTGGACCTATTGGAAGTGTTGAAATTTTATCATGAGAATGGTATTGATTGTACATTA
GAAGATGTGCAGTATAATGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILVMNVPEEFEDSEDIENVEDKI
EGYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDYIVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNG
TVFLKYLSKFKRDFAIEKVKKKERTDLI

Sequences:

>Translated_188_residues
MSVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILVMNVPEEFEDSEDIENVEDKI
EGYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDYIVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNG
TVFLKYLSKFKRDFAIEKVKKKERTDLI
>Mature_187_residues
SVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILVMNVPEEFEDSEDIENVEDKIE
GYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDYIVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNGT
VFLKYLSKFKRDFAIEKVKKKERTDLI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=164, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=9e-18,
Organism=Escherichia coli, GI1789682, Length=184, Percent_Identity=38.5869565217391, Blast_Score=134, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=32.2784810126582, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24645726, Length=143, Percent_Identity=32.1678321678322, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21568; Mature: 21437

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILV
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEE
MNVPEEFEDSEDIENVEDKIEGYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDY
EECCHHCCCCHHHHHHHHHHCCEEECCCCEEEECCEEECCCHHHHHHHHCCCCCCCHHHH
IVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNGTVFLKYLSKFKRDFAIEKVK
HCCCHHHEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KKERTDLI
HHHHCCCC
>Mature Secondary Structure 
SVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILV
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEE
MNVPEEFEDSEDIENVEDKIEGYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDY
EECCHHCCCCHHHHHHHHHHCCEEECCCCEEEECCEEECCCHHHHHHHHCCCCCCCHHHH
IVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNGTVFLKYLSKFKRDFAIEKVK
HCCCHHHEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KKERTDLI
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA