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The map label for this gene is tyrS

Identifier: 88658503

GI number: 88658503

Start: 81723

End: 82979

Strand: Direct

Name: tyrS

Synonym: ECH_0091

Alternate gene names: 88658503

Gene position: 81723-82979 (Clockwise)

Preceding gene: 88658224

Following gene: 88658613

Centisome position: 6.95

GC content: 31.5

Gene sequence:

>1257_bases
ATGAAGTTAGAGTCTCAATTCTTAAGTTTTTTGTATTCTCGAGGGTATTGTAGTCAATGCACTAATATTAGTATGTTGGA
TCAATTAATGAGCCAACAATGTGTACATGCATATATCGGGTTTGATTGTACTGCCAAAAGTTTGCATGTTGGTAGTTTAA
TACAAGTTATGATTTTAAGGCACCTTCAAAAATTTGGTCACAAACCAATAATACTACTGGGAGATGGTACAACTAAGATA
GGTGATCCTTCTGGTAAAGATAAATCTCGAGCTATGTTATCTGCTAGTGAAATTGAGGAAAATGCATTAGGTATCTATGA
GGTATTAAAGAAATTTATTGTATTTGGTGATGGACCTCATGATGCATTGTTAGTACGTAATGCTGAATGGTTAAATGGTT
TAAATTATATAGAATTTTTAAGAGATATAGGTAAACATTTTTCTGTTAACAATATGTTGACTTTTGATAGTGTAAGGTTG
CGTTTAGAAAGAGAACAAAATCTTAGCTTTCTAGAGTTTAATTACATGTTATTACAATCATATGATTTTGTTGAATTAAA
TCGACGGTATAATTGTTTATTACAAATAGGTGGATCTGATCAATGGGGTAATATTGTTAGTGGAGTAGAATTAGGCAGAA
AGTTAAGATTACCAGAGCTTTTTGGTCTTACTACTAATTTACTACTTACAAGCTCAGGTGAGAAGATGGGTAAGACTGCA
CAAGGTGCAGTGTGGCTTGATGGAAATATGTATAGTCCTGTAGATTATTGGCAATATTTTCGTAATGTTAAGGATGAGGA
TGTAGGTCGTTTTTTGAGGTTATTTACGGAATTATCGCTTAATGAAATAAGAAATTTAGAACTTTTGCAAGGACATGAGA
TTAATGAGTCGAAGAAGATATTAGCTACTGAAGCAACTAGGATATGTCATGGAGAAGAAATTGCTCAAAGTGTTGCTAAT
GATGCACTTAAAGTTTTTGAGTGCAATGATGATTCTGGATTATCAGTTTTTTATGTAAAGAAATATGATGTAGAATTAGG
GTTGCCTATAATAAAATTACTTCAAATGTGTGAAATGGAAAAAAGTAGTAGTAGTGCAAGAAGACTGATTAATGATAAGG
GATGTAAGATTAATGATGTGGTTGTATTAGATATGAATTATAAGTTATCCTTAAAAGACTTTTACAATACATCCTATGTT
AAATTAAGTTGTGGGAAGAAGCGTCATTTAAAAGTAATGTTAGAGAGTGATTTTTGA

Upstream 100 bases:

>100_bases
GATCCCGTAGCTCAGTCGGTAGAGCGTCTGACTTTTAATCAGCAGGTCGCGCGTTCAATTCGCGCCGGGATCACTGTTTT
TTTAATGATCTGTTTCTAAA

Downstream 100 bases:

>100_bases
TAGGGTTTTATATTAAAACATAGGATAGAGGTAATTATGATAGATTATGAAGAGATATTTTGTAGCAAGATTAAGCGTAT
AAAAGATGAAGGTCGATATA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKLESQFLSFLYSRGYCSQCTNISMLDQLMSQQCVHAYIGFDCTAKSLHVGSLIQVMILRHLQKFGHKPIILLGDGTTKI
GDPSGKDKSRAMLSASEIEENALGIYEVLKKFIVFGDGPHDALLVRNAEWLNGLNYIEFLRDIGKHFSVNNMLTFDSVRL
RLEREQNLSFLEFNYMLLQSYDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLRLPELFGLTTNLLLTSSGEKMGKTA
QGAVWLDGNMYSPVDYWQYFRNVKDEDVGRFLRLFTELSLNEIRNLELLQGHEINESKKILATEATRICHGEEIAQSVAN
DALKVFECNDDSGLSVFYVKKYDVELGLPIIKLLQMCEMEKSSSSARRLINDKGCKINDVVVLDMNYKLSLKDFYNTSYV
KLSCGKKRHLKVMLESDF

Sequences:

>Translated_418_residues
MKLESQFLSFLYSRGYCSQCTNISMLDQLMSQQCVHAYIGFDCTAKSLHVGSLIQVMILRHLQKFGHKPIILLGDGTTKI
GDPSGKDKSRAMLSASEIEENALGIYEVLKKFIVFGDGPHDALLVRNAEWLNGLNYIEFLRDIGKHFSVNNMLTFDSVRL
RLEREQNLSFLEFNYMLLQSYDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLRLPELFGLTTNLLLTSSGEKMGKTA
QGAVWLDGNMYSPVDYWQYFRNVKDEDVGRFLRLFTELSLNEIRNLELLQGHEINESKKILATEATRICHGEEIAQSVAN
DALKVFECNDDSGLSVFYVKKYDVELGLPIIKLLQMCEMEKSSSSARRLINDKGCKINDVVVLDMNYKLSLKDFYNTSYV
KLSCGKKRHLKVMLESDF
>Mature_418_residues
MKLESQFLSFLYSRGYCSQCTNISMLDQLMSQQCVHAYIGFDCTAKSLHVGSLIQVMILRHLQKFGHKPIILLGDGTTKI
GDPSGKDKSRAMLSASEIEENALGIYEVLKKFIVFGDGPHDALLVRNAEWLNGLNYIEFLRDIGKHFSVNNMLTFDSVRL
RLEREQNLSFLEFNYMLLQSYDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLRLPELFGLTTNLLLTSSGEKMGKTA
QGAVWLDGNMYSPVDYWQYFRNVKDEDVGRFLRLFTELSLNEIRNLELLQGHEINESKKILATEATRICHGEEIAQSVAN
DALKVFECNDDSGLSVFYVKKYDVELGLPIIKLLQMCEMEKSSSSARRLINDKGCKINDVVVLDMNYKLSLKDFYNTSYV
KLSCGKKRHLKVMLESDF

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=361, Percent_Identity=35.4570637119114, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=37.9474940334129, Blast_Score=277, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25145802, Length=301, Percent_Identity=34.5514950166113, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=210, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325160, Length=410, Percent_Identity=31.9512195121951, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI20130383, Length=387, Percent_Identity=36.1757105943152, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_EHRCR (Q2GI13)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_506920.1
- ProteinModelPortal:   Q2GI13
- SMR:   Q2GI13
- STRING:   Q2GI13
- GeneID:   3927142
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0091
- TIGR:   ECH_0091
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q2GI13
- ProtClustDB:   PRK05912
- BioCyc:   ECHA205920:ECH_0091-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47689; Mature: 47689

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 38-38 BINDING 175-175 BINDING 179-179 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESQFLSFLYSRGYCSQCTNISMLDQLMSQQCVHAYIGFDCTAKSLHVGSLIQVMILR
CCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HLQKFGHKPIILLGDGTTKIGDPSGKDKSRAMLSASEIEENALGIYEVLKKFIVFGDGPH
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCC
DALLVRNAEWLNGLNYIEFLRDIGKHFSVNNMLTFDSVRLRLEREQNLSFLEFNYMLLQS
CEEEEECHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHEEEECCCCCCEEEEEEHHHCC
YDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLRLPELFGLTTNLLLTSSGEKMGKTA
CCCEECCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHCCCEEEEEECCCHHHCCCC
QGAVWLDGNMYSPVDYWQYFRNVKDEDVGRFLRLFTELSLNEIRNLELLQGHEINESKKI
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCE
LATEATRICHGEEIAQSVANDALKVFECNDDSGLSVFYVKKYDVELGLPIIKLLQMCEME
EHHHHHHHHCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KSSSSARRLINDKGCKINDVVVLDMNYKLSLKDFYNTSYVKLSCGKKRHLKVMLESDF
CCCHHHHHHHCCCCCEEEEEEEEECCCEEEEHHHCCCCEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MKLESQFLSFLYSRGYCSQCTNISMLDQLMSQQCVHAYIGFDCTAKSLHVGSLIQVMILR
CCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HLQKFGHKPIILLGDGTTKIGDPSGKDKSRAMLSASEIEENALGIYEVLKKFIVFGDGPH
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCC
DALLVRNAEWLNGLNYIEFLRDIGKHFSVNNMLTFDSVRLRLEREQNLSFLEFNYMLLQS
CEEEEECHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHEEEECCCCCCEEEEEEHHHCC
YDFVELNRRYNCLLQIGGSDQWGNIVSGVELGRKLRLPELFGLTTNLLLTSSGEKMGKTA
CCCEECCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHCCCEEEEEECCCHHHCCCC
QGAVWLDGNMYSPVDYWQYFRNVKDEDVGRFLRLFTELSLNEIRNLELLQGHEINESKKI
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCE
LATEATRICHGEEIAQSVANDALKVFECNDDSGLSVFYVKKYDVELGLPIIKLLQMCEME
EHHHHHHHHCHHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KSSSSARRLINDKGCKINDVVVLDMNYKLSLKDFYNTSYVKLSCGKKRHLKVMLESDF
CCCHHHHHHHCCCCCEEEEEEEEECCCEEEEHHHCCCCEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA