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The map label for this gene is folD

Identifier: 88657832

GI number: 88657832

Start: 312256

End: 313158

Strand: Direct

Name: folD

Synonym: ECH_0324

Alternate gene names: 88657832

Gene position: 312256-313158 (Clockwise)

Preceding gene: 88658483

Following gene: 88658506

Centisome position: 26.55

GC content: 33.89

Gene sequence:

>903_bases
ATGGAAGAGAATATTATAAATGGAAAAGTCATCGCTGAGGATATTACTAGTACACTTGCTGTATGTATTAATGATTTAAA
AGTACAGCATAATGTAACACCATGTTTGATAGTTATACTTGTTGGTGATGATCCTGCAAGTCATTTGTATGTGCGCAATA
AACAGCGTAAAGCAGAGATGTTGGGACTAAGATCTGAAACTATGTTATTGCCAAGCACAACATCAGAAAGCAGTTTAATT
GATACAATACGTAGGTTAAATAATGATGATAGTGTTCATGGAATTTTAGTGCAGCTTCCTGTACCAAAGCATATTGATAA
GAATTTAATAATTAATACTATTGATCCAAGGAAGGATGTTGATGGATTCCATAATGAAAATGTTGGAAGGTTGTTTACTG
GACAAAAGAAAGATTGTCTAATACCTTGTACTCCTAGAGGATGTCTTTATTTGATTAAAACTGTAACTCGTAATTTATCT
GGAAGTGATGCTGTAGTTATAGGAAGGTCAAATATAGTAGGTAAACCTATGGCTTGTTTATTGTTGGGGGAAAATTGTAC
TGTTACAACAGTTCACTCAGCTACTCGTAACTTACCTGATTATTGTTCTAAAGCTGATGTTTTGGTAGCTGCTGTTGGTA
TACCTCATTTTGTTAAGTCTTCTTGGATTAAACCTGGAGCAATTGTTATAGATGTTGGTATTAATGCTATAGAGGAAGGT
GGAGTTAAAAAGTTTGTAGGAGATGTTGATTTTTCTGAAGCTAGAAATATAGCTTCAGCTATTACTCCGGTTCCTGGTGG
TGTAGGACCTATGACCATAGCATTTTTAATGGTTAATACTGTAATAGCAGCATGTAATCAAGCTGGTATTGAGAATTTTG
TTGAAAAGTATTTAGATTTGTAA

Upstream 100 bases:

>100_bases
TATAAATTATTGTTGCAGTTGGTATTAGTTATTAGTGTTTGTTGAAGTTTTATGTGAAATGTACTAGTCTGTAAATGATA
ATCACAATTTTTGGACTCTA

Downstream 100 bases:

>100_bases
TTCTTTAATTGCAGTAAATTCATTTATGGTTTATAACTAAATAATAAGGGCGGTTAGCTCAGTTGGTAGAGCGTCTCGTT
TACACCGAGAGGGTCAGCAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MEENIINGKVIAEDITSTLAVCINDLKVQHNVTPCLIVILVGDDPASHLYVRNKQRKAEMLGLRSETMLLPSTTSESSLI
DTIRRLNNDDSVHGILVQLPVPKHIDKNLIINTIDPRKDVDGFHNENVGRLFTGQKKDCLIPCTPRGCLYLIKTVTRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRNLPDYCSKADVLVAAVGIPHFVKSSWIKPGAIVIDVGINAIEEG
GVKKFVGDVDFSEARNIASAITPVPGGVGPMTIAFLMVNTVIAACNQAGIENFVEKYLDL

Sequences:

>Translated_300_residues
MEENIINGKVIAEDITSTLAVCINDLKVQHNVTPCLIVILVGDDPASHLYVRNKQRKAEMLGLRSETMLLPSTTSESSLI
DTIRRLNNDDSVHGILVQLPVPKHIDKNLIINTIDPRKDVDGFHNENVGRLFTGQKKDCLIPCTPRGCLYLIKTVTRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRNLPDYCSKADVLVAAVGIPHFVKSSWIKPGAIVIDVGINAIEEG
GVKKFVGDVDFSEARNIASAITPVPGGVGPMTIAFLMVNTVIAACNQAGIENFVEKYLDL
>Mature_300_residues
MEENIINGKVIAEDITSTLAVCINDLKVQHNVTPCLIVILVGDDPASHLYVRNKQRKAEMLGLRSETMLLPSTTSESSLI
DTIRRLNNDDSVHGILVQLPVPKHIDKNLIINTIDPRKDVDGFHNENVGRLFTGQKKDCLIPCTPRGCLYLIKTVTRNLS
GSDAVVIGRSNIVGKPMACLLLGENCTVTTVHSATRNLPDYCSKADVLVAAVGIPHFVKSSWIKPGAIVIDVGINAIEEG
GVKKFVGDVDFSEARNIASAITPVPGGVGPMTIAFLMVNTVIAACNQAGIENFVEKYLDL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=300, Percent_Identity=47.6666666666667, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=45.4237288135593, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=44.7457627118644, Blast_Score=216, Evalue=1e-56,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=29.2929292929293, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=49.1228070175439, Blast_Score=248, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=42.4137931034483, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=45.6375838926174, Blast_Score=257, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=42.0529801324503, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=314, Percent_Identity=26.4331210191083, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=45.2702702702703, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=45.2702702702703, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=44.636678200692, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=44.636678200692, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=44.636678200692, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=44.636678200692, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_EHRCR (Q2GHD8)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507145.1
- ProteinModelPortal:   Q2GHD8
- SMR:   Q2GHD8
- STRING:   Q2GHD8
- GeneID:   3927976
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0324
- TIGR:   ECH_0324
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q2GHD8
- ProtClustDB:   PRK14187
- BioCyc:   ECHA205920:ECH_0324-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32377; Mature: 32377

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENIINGKVIAEDITSTLAVCINDLKVQHNVTPCLIVILVGDDPASHLYVRNKQRKAEM
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECHHHHHHH
LGLRSETMLLPSTTSESSLIDTIRRLNNDDSVHGILVQLPVPKHIDKNLIINTIDPRKDV
HCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCC
DGFHNENVGRLFTGQKKDCLIPCTPRGCLYLIKTVTRNLSGSDAVVIGRSNIVGKPMACL
CCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEE
LLGENCTVTTVHSATRNLPDYCSKADVLVAAVGIPHFVKSSWIKPGAIVIDVGINAIEEG
EECCCCEEEEECHHHCCCHHHHCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCCHHHHC
GVKKFVGDVDFSEARNIASAITPVPGGVGPMTIAFLMVNTVIAACNQAGIENFVEKYLDL
CHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEENIINGKVIAEDITSTLAVCINDLKVQHNVTPCLIVILVGDDPASHLYVRNKQRKAEM
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECHHHHHHH
LGLRSETMLLPSTTSESSLIDTIRRLNNDDSVHGILVQLPVPKHIDKNLIINTIDPRKDV
HCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCC
DGFHNENVGRLFTGQKKDCLIPCTPRGCLYLIKTVTRNLSGSDAVVIGRSNIVGKPMACL
CCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEE
LLGENCTVTTVHSATRNLPDYCSKADVLVAAVGIPHFVKSSWIKPGAIVIDVGINAIEEG
EECCCCEEEEECHHHCCCHHHHCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCCHHHHC
GVKKFVGDVDFSEARNIASAITPVPGGVGPMTIAFLMVNTVIAACNQAGIENFVEKYLDL
CHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA