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The map label for this gene is sdhA [H]

Identifier: 88658480

GI number: 88658480

Start: 302203

End: 303999

Strand: Direct

Name: sdhA [H]

Synonym: ECH_0315

Alternate gene names: 88658480

Gene position: 302203-303999 (Clockwise)

Preceding gene: 88657695

Following gene: 88657846

Centisome position: 25.69

GC content: 37.95

Gene sequence:

>1797_bases
ATGTCTGGTTCTGCATATGATATTATAAATCATGAATATGATGTTGTAATAGTAGGAGCTGGAGGAGCAGGTTTAAGAGC
AACTTTTGGTATGACTAATGTTGGTTTATCAGTTGCATGTATATCTAAAGTTTTTCCTACACGAAGTCATACAGTAGCAG
CTCAAGGTGGTATTAGTGCTGCGTTAGGTAATGTTTCTGAAGATGACTGGAGATGGCATATGTTTGATACGGTCAAAGGT
TCAGATTGGCTTGGAGACCAAGATGCTATTGAATATATGTGTAAAAATGCGGCAGAAGCTGTAATTGAGCTTGAACGTTA
TGGAGTTCCTTTTTCTCGTACTAGTGATGGTAAAATATATCAGCGTCCTTTTGGTGGTATGACTACAGAGTTTGGTAAAG
GTAAGCCAGCAGTACGTACGTGTGCTGCATCTGATAAAACAGGTCATGCAATACTTCACACATTGTATCAGCAATCTTTA
AAGTATAATGCTAAGTTTTTTGTAGAGTATTTTGCTATTGATTTAATAATGGATGAAGAAGGCCAGTGTAAGGGTGTATT
AGCATGGTCTTTGTGTGATGGTACGTTACATAGATTTCGTGCTCATATTGTTGTTCTTGCTACAGGTGGTTATGGAAGGA
TATATTTTTCTGCTACTAGTGCACACACTTGTACTGGTGATGGGGGAGGTATGGTATCCCGTATTAATTTGCCGCTGGAA
GATATGGAATTTGTACAATTTCACCCGACTGGAATATATGGTGCTGGATGTTTGATAACAGAAGGTTGCAGAGGGGAAGG
TGGATATTTAATTAATTCTGAGGGAGAAAGATTTATGGAGCGCTATGCTCCAAAAGCTAAAGATTTGGCATCTCGTGATG
TTGTTAGTAGAGCAATGACTTTAGAGATTAGAGAAGGTCGTGGTGTTGGTCCAAAAAAAGATCATGTATATTTGTCTATA
TCTCATTTAGGTGCAAAAGTTATCAGTGAAAGATTGCCAGGTATTCGTGAGACTGCACGTACTTTTGCTGGAGTAGATGT
TATAAAGGAGCCAATACCAGTTTTACCAACTGTTCATTACAATATGGGGGGTATCCCTACAAATTATCATGGTGAAGTTG
TAACTTTAAGCAATAATACAGAGCAGATAATACCTGGGCTTTTTGCTATAGGAGAAGCAGCGTGTGTATCTGTACATGGT
GCTAACCGTTTAGGGTCTAATTCATTGCTTGATTTAGTAGTTTTTGGAAGAGCTGCAGCAATTAGAGCAAAAGAGTTAAT
CAAACCCGGAATGTTACATGCTCCAGTGCAGAAGTCATCAGAAGAGTGGATAATTGATAGGTTTGATGCGTTAAGGTTTT
CAAAAGGAAGTTTACGTGTATCAGAAATACGTAGTAACATGCAAAATGTAATGCAAAATCATGCAGCTGTTTTCCGTACA
GAAGAAGTGCTTGAAGAAGGCAAGCAAAAGATAAGAAGTGTTGCTGAGTCTCTGTCTGAAATTGCGGTTAAAGATAGAAG
TATGATATGGAATAGTGATTTGGTTGAAGCATTAGAGTTAACTAATATGATGCCGCAAGCAGTCGTAACAATGGAATGTG
CTGCAAATCGTCAAGAAAGTAGAGGGGCTCATGCACGTGAAGATTTCCCTGATCGTGATGATGAGAATTGGATGAAACAT
AGCTTAGCATGGTATAATTCTACAGATTGTTCTGTACATATAAAGTATAAAGATGTTGCTAAAGCAACTTTAACAGATGA
TGTTCAGTATTTTCCTCCACAAAAAAGAGTATATTAA

Upstream 100 bases:

>100_bases
CAAGTTATATTGTGAAGTTATTTAACATAGGAATTATATTGACTCATTGTGTTATTGTGGTAGCCTAATTATATTTATTA
TTTAAGTATTATTTATTGTA

Downstream 100 bases:

>100_bases
TACTATCTATATAGTTATATGGTGGTTATTTAAAAGGTTTGACTATGGTTCAATTTTTTTTACCTAAGAATTCTAAAGTT
AATAAGAACGGTAAGGTTCA

Product: succinate dehydrogenase flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSGSAYDIINHEYDVVIVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISAALGNVSEDDWRWHMFDTVKG
SDWLGDQDAIEYMCKNAAEAVIELERYGVPFSRTSDGKIYQRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSL
KYNAKFFVEYFAIDLIMDEEGQCKGVLAWSLCDGTLHRFRAHIVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLE
DMEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMTLEIREGRGVGPKKDHVYLSI
SHLGAKVISERLPGIRETARTFAGVDVIKEPIPVLPTVHYNMGGIPTNYHGEVVTLSNNTEQIIPGLFAIGEAACVSVHG
ANRLGSNSLLDLVVFGRAAAIRAKELIKPGMLHAPVQKSSEEWIIDRFDALRFSKGSLRVSEIRSNMQNVMQNHAAVFRT
EEVLEEGKQKIRSVAESLSEIAVKDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQESRGAHAREDFPDRDDENWMKH
SLAWYNSTDCSVHIKYKDVAKATLTDDVQYFPPQKRVY

Sequences:

>Translated_598_residues
MSGSAYDIINHEYDVVIVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISAALGNVSEDDWRWHMFDTVKG
SDWLGDQDAIEYMCKNAAEAVIELERYGVPFSRTSDGKIYQRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSL
KYNAKFFVEYFAIDLIMDEEGQCKGVLAWSLCDGTLHRFRAHIVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLE
DMEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMTLEIREGRGVGPKKDHVYLSI
SHLGAKVISERLPGIRETARTFAGVDVIKEPIPVLPTVHYNMGGIPTNYHGEVVTLSNNTEQIIPGLFAIGEAACVSVHG
ANRLGSNSLLDLVVFGRAAAIRAKELIKPGMLHAPVQKSSEEWIIDRFDALRFSKGSLRVSEIRSNMQNVMQNHAAVFRT
EEVLEEGKQKIRSVAESLSEIAVKDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQESRGAHAREDFPDRDDENWMKH
SLAWYNSTDCSVHIKYKDVAKATLTDDVQYFPPQKRVY
>Mature_597_residues
SGSAYDIINHEYDVVIVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISAALGNVSEDDWRWHMFDTVKGS
DWLGDQDAIEYMCKNAAEAVIELERYGVPFSRTSDGKIYQRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSLK
YNAKFFVEYFAIDLIMDEEGQCKGVLAWSLCDGTLHRFRAHIVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLED
MEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMTLEIREGRGVGPKKDHVYLSIS
HLGAKVISERLPGIRETARTFAGVDVIKEPIPVLPTVHYNMGGIPTNYHGEVVTLSNNTEQIIPGLFAIGEAACVSVHGA
NRLGSNSLLDLVVFGRAAAIRAKELIKPGMLHAPVQKSSEEWIIDRFDALRFSKGSLRVSEIRSNMQNVMQNHAAVFRTE
EVLEEGKQKIRSVAESLSEIAVKDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQESRGAHAREDFPDRDDENWMKHS
LAWYNSTDCSVHIKYKDVAKATLTDDVQYFPPQKRVY

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=613, Percent_Identity=60.6851549755302, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1786942, Length=592, Percent_Identity=53.5472972972973, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790597, Length=578, Percent_Identity=42.0415224913495, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1788928, Length=556, Percent_Identity=31.4748201438849, Blast_Score=218, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17550100, Length=612, Percent_Identity=58.3333333333333, Blast_Score=711, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17505833, Length=615, Percent_Identity=57.0731707317073, Blast_Score=694, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322701, Length=596, Percent_Identity=62.751677852349, Blast_Score=776, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322416, Length=598, Percent_Identity=62.876254180602, Blast_Score=765, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320788, Length=492, Percent_Identity=27.8455284552846, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137288, Length=611, Percent_Identity=60.7201309328969, Blast_Score=754, Evalue=0.0,
Organism=Drosophila melanogaster, GI24655642, Length=611, Percent_Identity=60.7201309328969, Blast_Score=754, Evalue=0.0,
Organism=Drosophila melanogaster, GI24655647, Length=611, Percent_Identity=60.7201309328969, Blast_Score=754, Evalue=0.0,
Organism=Drosophila melanogaster, GI24663005, Length=613, Percent_Identity=55.9543230016313, Blast_Score=691, Evalue=0.0,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR011281
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 65831; Mature: 65699

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSAYDIINHEYDVVIVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISA
CCCCEEEECCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCHH
ALGNVSEDDWRWHMFDTVKGSDWLGDQDAIEYMCKNAAEAVIELERYGVPFSRTSDGKIY
HHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
QRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSLKYNAKFFVEYFAIDLIMDEE
ECCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCC
GQCKGVLAWSLCDGTLHRFRAHIVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLE
CCCCEEEEEEHHHHHHHHEEEEEEEEEECCCEEEEEEECCCCEEECCCCCEEEEECCCCC
DMEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMT
CCCEEEECCCCEEECCEEEECCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHEE
LEIREGRGVGPKKDHVYLSISHLGAKVISERLPGIRETARTFAGVDVIKEPIPVLPTVHY
EEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
NMGGIPTNYHGEVVTLSNNTEQIIPGLFAIGEAACVSVHGANRLGSNSLLDLVVFGRAAA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
IRAKELIKPGMLHAPVQKSSEEWIIDRFDALRFSKGSLRVSEIRSNMQNVMQNHAAVFRT
HHHHHHCCCCCEECCCCCCCCHHHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHHHHH
EEVLEEGKQKIRSVAESLSEIAVKDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEEHHHHCCCHHH
RGAHAREDFPDRDDENWMKHSLAWYNSTDCSVHIKYKDVAKATLTDDVQYFPPQKRVY
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEEEEEHHHHHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
SGSAYDIINHEYDVVIVGAGGAGLRATFGMTNVGLSVACISKVFPTRSHTVAAQGGISA
CCCEEEECCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCHH
ALGNVSEDDWRWHMFDTVKGSDWLGDQDAIEYMCKNAAEAVIELERYGVPFSRTSDGKIY
HHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
QRPFGGMTTEFGKGKPAVRTCAASDKTGHAILHTLYQQSLKYNAKFFVEYFAIDLIMDEE
ECCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCC
GQCKGVLAWSLCDGTLHRFRAHIVVLATGGYGRIYFSATSAHTCTGDGGGMVSRINLPLE
CCCCEEEEEEHHHHHHHHEEEEEEEEEECCCEEEEEEECCCCEEECCCCCEEEEECCCCC
DMEFVQFHPTGIYGAGCLITEGCRGEGGYLINSEGERFMERYAPKAKDLASRDVVSRAMT
CCCEEEECCCCEEECCEEEECCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHEE
LEIREGRGVGPKKDHVYLSISHLGAKVISERLPGIRETARTFAGVDVIKEPIPVLPTVHY
EEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
NMGGIPTNYHGEVVTLSNNTEQIIPGLFAIGEAACVSVHGANRLGSNSLLDLVVFGRAAA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
IRAKELIKPGMLHAPVQKSSEEWIIDRFDALRFSKGSLRVSEIRSNMQNVMQNHAAVFRT
HHHHHHCCCCCEECCCCCCCCHHHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHHHHH
EEVLEEGKQKIRSVAESLSEIAVKDRSMIWNSDLVEALELTNMMPQAVVTMECAANRQES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEEHHHHCCCHHH
RGAHAREDFPDRDDENWMKHSLAWYNSTDCSVHIKYKDVAKATLTDDVQYFPPQKRVY
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCEEEEEEHHHHHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA