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The map label for this gene is truB

Identifier: 88658449

GI number: 88658449

Start: 738289

End: 739179

Strand: Reverse

Name: truB

Synonym: ECH_0728

Alternate gene names: 88658449

Gene position: 739179-738289 (Counterclockwise)

Preceding gene: 88658023

Following gene: 88657975

Centisome position: 62.84

GC content: 31.54

Gene sequence:

>891_bases
ATGTATGGTTGGATAAACCTAGATAAGCCATGTGGTATAAGTTCTGCTTCTGCAGTTAATCTAGTTAAAAAAATTTTAAA
TGTCAAAAAAGTTGGTCATGCAGGTACATTAGATCCTTTAGCTTCTGGTGTGTTGCCCATTGCTATTGGTGAAGCTACAA
AAGTTATGCCTTATGCTGTTGATGTTGTGAAATCTTATTTGTTTACAGTGCAATGGGGTGAGCAAAGAACAACTGATGAT
ATAGAAGGAAAGATAGTAGCAAAAAGTGATATAGTACCTAGTTCAGAAGATATTAAAAAAGCTATTCCAAATTTTATTGG
ATTGTTAAGACAAGTACCTCCTAATTTTTCAGCAGTACATGTGAATGGAGTAAGAGCTTTTAAATTGGCGAGAAATGGTC
AAGATGTAAGTTTAACTTCTAGAGATGTTAACGTGTTAAAATTGAAATTATTGTCTGCTGATCAAGAGAATAACAAAGCA
GATTTTTATTTATTATGTAAGAAAGGCGTATATGTTCGTTCTATAGCAAGGGATTTAGGAATAGAGTTAGGATGTTTGGG
GTATGTTAGAAAATTACAGAGAGTTAGGGTAGGTTGTTTTAGGAAGAAAAATGCCATTACTTTGGAAATGTTAAAGATGT
TATATAATAATAGTAGAAAATGTAGTTATTTGTTACCTTTATGGTATGTTTTGCAGGATATGAAACATCTTAATGATGTG
TTTTCTGATATTCAGATAAAAAAGATTAAAAATGGTCAAAATATTGAGTTAAATAACTTGTATGTAGTAAGGAATTGTGG
TATCTGTTATGTTAGTACGGGTAATGTGCCAGTGGCAATCTGTAGTATAGTTAATAGTGTTGTAAGGCCAGTACGTATTT
TTAATGTTTAA

Upstream 100 bases:

>100_bases
TTTAGATGTTTTGGTTTTTATTATTTAATGGTAACTATGTAATTTTAATGCCTATATAATGTTTCCGTAGGTTATTAAGT
TGATTTGAATTTTTAATTGT

Downstream 100 bases:

>100_bases
AGATTTGGTATTTTAGTTATGTCAATTACACGTGAAAGAAAGTCTGAATTAATAAGTGAATATTGCCTTAAAAAAGATGA
TACTGGTTCTTCTTTTGTAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MYGWINLDKPCGISSASAVNLVKKILNVKKVGHAGTLDPLASGVLPIAIGEATKVMPYAVDVVKSYLFTVQWGEQRTTDD
IEGKIVAKSDIVPSSEDIKKAIPNFIGLLRQVPPNFSAVHVNGVRAFKLARNGQDVSLTSRDVNVLKLKLLSADQENNKA
DFYLLCKKGVYVRSIARDLGIELGCLGYVRKLQRVRVGCFRKKNAITLEMLKMLYNNSRKCSYLLPLWYVLQDMKHLNDV
FSDIQIKKIKNGQNIELNNLYVVRNCGICYVSTGNVPVAICSIVNSVVRPVRIFNV

Sequences:

>Translated_296_residues
MYGWINLDKPCGISSASAVNLVKKILNVKKVGHAGTLDPLASGVLPIAIGEATKVMPYAVDVVKSYLFTVQWGEQRTTDD
IEGKIVAKSDIVPSSEDIKKAIPNFIGLLRQVPPNFSAVHVNGVRAFKLARNGQDVSLTSRDVNVLKLKLLSADQENNKA
DFYLLCKKGVYVRSIARDLGIELGCLGYVRKLQRVRVGCFRKKNAITLEMLKMLYNNSRKCSYLLPLWYVLQDMKHLNDV
FSDIQIKKIKNGQNIELNNLYVVRNCGICYVSTGNVPVAICSIVNSVVRPVRIFNV
>Mature_296_residues
MYGWINLDKPCGISSASAVNLVKKILNVKKVGHAGTLDPLASGVLPIAIGEATKVMPYAVDVVKSYLFTVQWGEQRTTDD
IEGKIVAKSDIVPSSEDIKKAIPNFIGLLRQVPPNFSAVHVNGVRAFKLARNGQDVSLTSRDVNVLKLKLLSADQENNKA
DFYLLCKKGVYVRSIARDLGIELGCLGYVRKLQRVRVGCFRKKNAITLEMLKMLYNNSRKCSYLLPLWYVLQDMKHLNDV
FSDIQIKKIKNGQNIELNNLYVVRNCGICYVSTGNVPVAICSIVNSVVRPVRIFNV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=294, Percent_Identity=28.2312925170068, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=29.5019157088123, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4503337, Length=261, Percent_Identity=29.5019157088123, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI2367200, Length=218, Percent_Identity=36.697247706422, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553978, Length=226, Percent_Identity=29.2035398230088, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=226, Percent_Identity=30.5309734513274, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=140, Percent_Identity=36.4285714285714, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364187, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364185, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364183, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI62471759, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17975520, Length=227, Percent_Identity=30.3964757709251, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_EHRCR (Q2GGA2)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507531.1
- ProteinModelPortal:   Q2GGA2
- SMR:   Q2GGA2
- STRING:   Q2GGA2
- GeneID:   3927663
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0728
- TIGR:   ECH_0728
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   Q2GGA2
- ProtClustDB:   CLSK2516851
- BioCyc:   ECHA205920:ECH_0728-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 32930; Mature: 32930

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGWINLDKPCGISSASAVNLVKKILNVKKVGHAGTLDPLASGVLPIAIGEATKVMPYAV
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCHHHHHHHH
DVVKSYLFTVQWGEQRTTDDIEGKIVAKSDIVPSSEDIKKAIPNFIGLLRQVPPNFSAVH
HHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
VNGVRAFKLARNGQDVSLTSRDVNVLKLKLLSADQENNKADFYLLCKKGVYVRSIARDLG
ECCEEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHC
IELGCLGYVRKLQRVRVGCFRKKNAITLEMLKMLYNNSRKCSYLLPLWYVLQDMKHLNDV
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
FSDIQIKKIKNGQNIELNNLYVVRNCGICYVSTGNVPVAICSIVNSVVRPVRIFNV
HHCCEEEEECCCCCEEECCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MYGWINLDKPCGISSASAVNLVKKILNVKKVGHAGTLDPLASGVLPIAIGEATKVMPYAV
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCHHHHHHHH
DVVKSYLFTVQWGEQRTTDDIEGKIVAKSDIVPSSEDIKKAIPNFIGLLRQVPPNFSAVH
HHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
VNGVRAFKLARNGQDVSLTSRDVNVLKLKLLSADQENNKADFYLLCKKGVYVRSIARDLG
ECCEEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHC
IELGCLGYVRKLQRVRVGCFRKKNAITLEMLKMLYNNSRKCSYLLPLWYVLQDMKHLNDV
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
FSDIQIKKIKNGQNIELNNLYVVRNCGICYVSTGNVPVAICSIVNSVVRPVRIFNV
HHCCEEEEECCCCCEEECCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA