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The map label for this gene is recJ [H]

Identifier: 88658434

GI number: 88658434

Start: 1138293

End: 1140062

Strand: Direct

Name: recJ [H]

Synonym: ECH_1115

Alternate gene names: 88658434

Gene position: 1138293-1140062 (Clockwise)

Preceding gene: 88657689

Following gene: 88657671

Centisome position: 96.77

GC content: 32.66

Gene sequence:

>1770_bases
ATGCTACATTGCAATTTGGAAAATTTAGAATACATTGGGGTAACAGGTGCACTATGGAAGCCATATAATGTAAACTTCCG
TGATGTTTTAACAATAACACAAAAACTTGGTATATCTGAAATTGTTGCAAGAGTATTATCAACAAGAAAAGTAAATATAG
AAGAGATCAATGACTTTCTATATCCTACACTAAGGTCATCTTTACCTGATCCTTTCCATCTTTTAGATATGGACAAAGCA
ATACAGAGAATTTGTCACGCAATATATAATAAAGAAAGAATTGTTATTTTTGGAGATTATGATGTTGATGGAGCAACATC
ATCTGCATTAATAAAGCAATACTTAACACAAATAGGTGTACCTACAACAATATATATTCCAGATCGTATATGTGAAGGAT
ACGGACCAAACACTCAAGCCTTACTTAAATTACAAGAAATTGGGCACAGTTTATGCATAACTGTTGATTGTGGGACTATA
GCACATGAACCAATATCCGTAGCAAAATCTGTAAATCTTGATGTGATAGTTATAGACCATCATATGGGCTTGAACACTTT
ACCAGATGCAATTGCAGTTATTAATCCCAATCGCCTAGATGAAAATTCTCCATATACATATTTAGCAGGAGTTGGTGTAT
CATTTTTAATGCTTGTAGCACTAAATAAAACTTTAAAAGAAAAAGGATTTTTTACAAATAACAACGAACCAAATTTAATA
AACTATCTAGATTTAGTAGCATTGGGAACTGTATGTGATGTCATGCCTATTATAGGATTAAATAGAGCTTTCGTAAAACA
AGGCTTAAAAATTATGGCAACAAGACAAAATATAGGCCTTAAAGTCTTGTCTGATGTTATTGGTTTAGAAGATAAACCAA
ATACTTACCAACTAGGATTTAATATAGGTCCACATATTAATGCTGGAGGAAGAGTTGGAAACGCAAGTTTAGGAGCAAGG
CTATTATCAAGTAATAGTGAAGAAGAAGCTCTAGAAATTTCAGAAAAATTACAAAACTTTAATCTAGAAAGAAAAGAGTT
AGAACATAGAAGTTTCAATGAAGCAGTAGAACAAGCAGAAGCTCTAATATCAACAAATACAAATTTAATTATAGTTGCAG
GAAACTGGCACCCAGGAATAATAGGTATAGTCGCAGGAAGACTTAAAGATAAATTTTTTCTACCAAGCATAGTAATTTCA
CTGGATAAGGGCATTGGCAAAGCGAGCGCTAGATCAATACCAGATGTAGACTTGGGTGCAGCCATTTTATCAGCAAAATT
AGAAGGGATCATCACTGAAGGAGGAGGACATGCCATGGCTGCTGGATTCTCAATACAGGAAGATAAAATTAAAACATTAA
ATGAATTCCTTTCACAAAGATTTCACAATATTAATAAACAAAAAGTATTTAAAGTAGACGGCATTATTACTGCAGCAGCA
GTTAATCTAACCTTATGGAAAGAATTACAGTTTTTAGAACCCTTTGGCATAGGCAATCCTGAACCAAGGTTTATCCTGAC
AAATATCAAAATAAAAAATCCGGAGATTATAGGAGATAGTCATATAAGATGTTTAATTCATGACAACAAAACTTTTATAA
AAGGAATTTGTTTCAGATGTACAGACACAGAATTGGGCTTAGCTCTATTAGAACGTACTGCAATCATGTTACTAGGAAAA
ATCAGCGTTAACTATTGGAAAGGCAATGAGAACATACAGTTTATAATAGAAGATGCGATTACAAAAAGTCACAATGATCA
CAAATTATAA

Upstream 100 bases:

>100_bases
CTTTACAAAATCTTAAAATTATAAACACAAACACACTAAAATAATGTTATAGTAAGTATAAAATAGCAGTGTTATAATAC
ATATTTTTAGTTAACACAGT

Downstream 100 bases:

>100_bases
AGATTATAGGAATACTCAACACAAATGTTGTTATTGGTAGATAAATTCAATAATACATAACCTTTACAATCTACAAGGAC
ATTAAATAGCAAAAATATTA

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MLHCNLENLEYIGVTGALWKPYNVNFRDVLTITQKLGISEIVARVLSTRKVNIEEINDFLYPTLRSSLPDPFHLLDMDKA
IQRICHAIYNKERIVIFGDYDVDGATSSALIKQYLTQIGVPTTIYIPDRICEGYGPNTQALLKLQEIGHSLCITVDCGTI
AHEPISVAKSVNLDVIVIDHHMGLNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLVALNKTLKEKGFFTNNNEPNLI
NYLDLVALGTVCDVMPIIGLNRAFVKQGLKIMATRQNIGLKVLSDVIGLEDKPNTYQLGFNIGPHINAGGRVGNASLGAR
LLSSNSEEEALEISEKLQNFNLERKELEHRSFNEAVEQAEALISTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVIS
LDKGIGKASARSIPDVDLGAAILSAKLEGIITEGGGHAMAAGFSIQEDKIKTLNEFLSQRFHNINKQKVFKVDGIITAAA
VNLTLWKELQFLEPFGIGNPEPRFILTNIKIKNPEIIGDSHIRCLIHDNKTFIKGICFRCTDTELGLALLERTAIMLLGK
ISVNYWKGNENIQFIIEDAITKSHNDHKL

Sequences:

>Translated_589_residues
MLHCNLENLEYIGVTGALWKPYNVNFRDVLTITQKLGISEIVARVLSTRKVNIEEINDFLYPTLRSSLPDPFHLLDMDKA
IQRICHAIYNKERIVIFGDYDVDGATSSALIKQYLTQIGVPTTIYIPDRICEGYGPNTQALLKLQEIGHSLCITVDCGTI
AHEPISVAKSVNLDVIVIDHHMGLNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLVALNKTLKEKGFFTNNNEPNLI
NYLDLVALGTVCDVMPIIGLNRAFVKQGLKIMATRQNIGLKVLSDVIGLEDKPNTYQLGFNIGPHINAGGRVGNASLGAR
LLSSNSEEEALEISEKLQNFNLERKELEHRSFNEAVEQAEALISTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVIS
LDKGIGKASARSIPDVDLGAAILSAKLEGIITEGGGHAMAAGFSIQEDKIKTLNEFLSQRFHNINKQKVFKVDGIITAAA
VNLTLWKELQFLEPFGIGNPEPRFILTNIKIKNPEIIGDSHIRCLIHDNKTFIKGICFRCTDTELGLALLERTAIMLLGK
ISVNYWKGNENIQFIIEDAITKSHNDHKL
>Mature_589_residues
MLHCNLENLEYIGVTGALWKPYNVNFRDVLTITQKLGISEIVARVLSTRKVNIEEINDFLYPTLRSSLPDPFHLLDMDKA
IQRICHAIYNKERIVIFGDYDVDGATSSALIKQYLTQIGVPTTIYIPDRICEGYGPNTQALLKLQEIGHSLCITVDCGTI
AHEPISVAKSVNLDVIVIDHHMGLNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLVALNKTLKEKGFFTNNNEPNLI
NYLDLVALGTVCDVMPIIGLNRAFVKQGLKIMATRQNIGLKVLSDVIGLEDKPNTYQLGFNIGPHINAGGRVGNASLGAR
LLSSNSEEEALEISEKLQNFNLERKELEHRSFNEAVEQAEALISTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVIS
LDKGIGKASARSIPDVDLGAAILSAKLEGIITEGGGHAMAAGFSIQEDKIKTLNEFLSQRFHNINKQKVFKVDGIITAAA
VNLTLWKELQFLEPFGIGNPEPRFILTNIKIKNPEIIGDSHIRCLIHDNKTFIKGICFRCTDTELGLALLERTAIMLLGK
ISVNYWKGNENIQFIIEDAITKSHNDHKL

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=524, Percent_Identity=33.7786259541985, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 65062; Mature: 65062

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHCNLENLEYIGVTGALWKPYNVNFRDVLTITQKLGISEIVARVLSTRKVNIEEINDFL
CEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
YPTLRSSLPDPFHLLDMDKAIQRICHAIYNKERIVIFGDYDVDGATSSALIKQYLTQIGV
HHHHHHCCCCCEEHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
PTTIYIPDRICEGYGPNTQALLKLQEIGHSLCITVDCGTIAHEPISVAKSVNLDVIVIDH
CEEEEECHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEEC
HMGLNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLVALNKTLKEKGFFTNNNEPNLI
CCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
NYLDLVALGTVCDVMPIIGLNRAFVKQGLKIMATRQNIGLKVLSDVIGLEDKPNTYQLGF
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEC
NIGPHINAGGRVGNASLGARLLSSNSEEEALEISEKLQNFNLERKELEHRSFNEAVEQAE
CCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ALISTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVISLDKGIGKASARSIPDVDLGA
HHHCCCCCEEEEECCCCCCCCEEEHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AILSAKLEGIITEGGGHAMAAGFSIQEDKIKTLNEFLSQRFHNINKQKVFKVDGIITAAA
HHHHHHHCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCEEEEHH
VNLTLWKELQFLEPFGIGNPEPRFILTNIKIKNPEIIGDSHIRCLIHDNKTFIKGICFRC
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEE
TDTELGLALLERTAIMLLGKISVNYWKGNENIQFIIEDAITKSHNDHKL
CCCHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEHHHHCCCCCCCC
>Mature Secondary Structure
MLHCNLENLEYIGVTGALWKPYNVNFRDVLTITQKLGISEIVARVLSTRKVNIEEINDFL
CEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
YPTLRSSLPDPFHLLDMDKAIQRICHAIYNKERIVIFGDYDVDGATSSALIKQYLTQIGV
HHHHHHCCCCCEEHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
PTTIYIPDRICEGYGPNTQALLKLQEIGHSLCITVDCGTIAHEPISVAKSVNLDVIVIDH
CEEEEECHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEEC
HMGLNTLPDAIAVINPNRLDENSPYTYLAGVGVSFLMLVALNKTLKEKGFFTNNNEPNLI
CCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
NYLDLVALGTVCDVMPIIGLNRAFVKQGLKIMATRQNIGLKVLSDVIGLEDKPNTYQLGF
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEC
NIGPHINAGGRVGNASLGARLLSSNSEEEALEISEKLQNFNLERKELEHRSFNEAVEQAE
CCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ALISTNTNLIIVAGNWHPGIIGIVAGRLKDKFFLPSIVISLDKGIGKASARSIPDVDLGA
HHHCCCCCEEEEECCCCCCCCEEEHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AILSAKLEGIITEGGGHAMAAGFSIQEDKIKTLNEFLSQRFHNINKQKVFKVDGIITAAA
HHHHHHHCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEECCEEEEHH
VNLTLWKELQFLEPFGIGNPEPRFILTNIKIKNPEIIGDSHIRCLIHDNKTFIKGICFRC
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEE
TDTELGLALLERTAIMLLGKISVNYWKGNENIQFIIEDAITKSHNDHKL
CCCHHHHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]