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The map label for this gene is cca [H]

Identifier: 88657671

GI number: 88657671

Start: 1140567

End: 1141772

Strand: Direct

Name: cca [H]

Synonym: ECH_1116

Alternate gene names: 88657671

Gene position: 1140567-1141772 (Clockwise)

Preceding gene: 88658434

Following gene: 88657852

Centisome position: 96.97

GC content: 30.27

Gene sequence:

>1206_bases
ATGTACGATGACTTTATAAATAATGAGAATATTCTGTTAATCATAAATGCAATAAAAAAATTTCAAGGAGATATTAGGTT
AGTAGGTGGATGTGTAAGAGATAGTCTTCTAAAAAGACAGACCATAGATATTGATTTTGCAACTACTTTATTACCCAACC
AAACAATAAATGCTCTTACTGCAGCTCATATTAAAGCTATTCCAACAGGTATAAAACATGGTACAATAACAGCCTTAGTT
AATAATACAGCATATGAAATTACAACACTAAGATCTGATATTAGCTGTGATGGAAGACATGCTGAAGTAAAATTTACAAA
CAATTGGCAGCAAGACGCTTCAAGAAGAGATTTCACCTTCAATGCTCTATATTGTGATGAAAAAGGAATAGTATATGATT
ATTTTTCTGGTATCCAAGATCTAGAAAAAAAACATCTAAATTTTATTGGAGATCCAGAAATTAGAATACAAGAAGACTAC
CTACGCATACTTCGAGCATTTAGATTTTATGCTTCTATATGTAGTCAAAACAAATTGAGTGATGAAATAGTGCACTCTTG
CACAAAATATTCATCTTATATCAATAACCTATCCAGAGAACGCATTCGCGATGAGTTCTTTAAACTTTTATTATGTCCTA
ACTTATCAAACACATTAAAGATTATGCAAAAATGCCACGTGCTAGATAAAATCATTCCCTTTGAAGTCATACCAGACATA
ATGTCATCTGAGACCTTATCAAACACAGATCCACTAACAAAATTAGCAGCTCTTTTAAGAACAAACAATAACAACCACTC
TCTAGATAAAATTAAAGCTTCTTTATGCTTATCAAACTACAGTCAAAAAACACTTGTGTCACTATTAAACAATAATTTAG
AACTTCCACTTTCAACTACCGCACAACACAAACACATTAACAAGCTTGGAAAAGAAATATACTGCAATCTACTGAGAATA
ATACATGCAGAATTAAATTTAAATTATCATGACCTAATGCAATATATAGAGTACGCAGATCAATTAATTATTCCTGAATT
TCCTATCTCTGGAAAAGATTTACTTAATATAGGATACCAACCAGGAAAAAATCTTGGTATCACTTTAGAAAAAATCAAAG
ATCTATGGGAAAATAGTTCATATCAACTAACAAAAACCCAATTATTAGATTACGCGAGAGGAAAATTATTAAAAAGTAAG
AATTAA

Upstream 100 bases:

>100_bases
ATGTACACAAATAATTAAAAATTAATAGGATTTAAAAAAACAATGCAATACTATCAGTATTTGTTTAACATTTTACTGTT
TCAACAATCTAACGTTACAA

Downstream 100 bases:

>100_bases
ATCTCGTACTATCTTCAAAAAATTACATAAGATAAAAACATTTTATTGCAAACTGTATTTTACAAGAAATTACATAGCTA
TTTCCAAACACACTAAAATA

Product: polyA polymerase/tRNA nucleotidyltransferase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MYDDFINNENILLIINAIKKFQGDIRLVGGCVRDSLLKRQTIDIDFATTLLPNQTINALTAAHIKAIPTGIKHGTITALV
NNTAYEITTLRSDISCDGRHAEVKFTNNWQQDASRRDFTFNALYCDEKGIVYDYFSGIQDLEKKHLNFIGDPEIRIQEDY
LRILRAFRFYASICSQNKLSDEIVHSCTKYSSYINNLSRERIRDEFFKLLLCPNLSNTLKIMQKCHVLDKIIPFEVIPDI
MSSETLSNTDPLTKLAALLRTNNNNHSLDKIKASLCLSNYSQKTLVSLLNNNLELPLSTTAQHKHINKLGKEIYCNLLRI
IHAELNLNYHDLMQYIEYADQLIIPEFPISGKDLLNIGYQPGKNLGITLEKIKDLWENSSYQLTKTQLLDYARGKLLKSK
N

Sequences:

>Translated_401_residues
MYDDFINNENILLIINAIKKFQGDIRLVGGCVRDSLLKRQTIDIDFATTLLPNQTINALTAAHIKAIPTGIKHGTITALV
NNTAYEITTLRSDISCDGRHAEVKFTNNWQQDASRRDFTFNALYCDEKGIVYDYFSGIQDLEKKHLNFIGDPEIRIQEDY
LRILRAFRFYASICSQNKLSDEIVHSCTKYSSYINNLSRERIRDEFFKLLLCPNLSNTLKIMQKCHVLDKIIPFEVIPDI
MSSETLSNTDPLTKLAALLRTNNNNHSLDKIKASLCLSNYSQKTLVSLLNNNLELPLSTTAQHKHINKLGKEIYCNLLRI
IHAELNLNYHDLMQYIEYADQLIIPEFPISGKDLLNIGYQPGKNLGITLEKIKDLWENSSYQLTKTQLLDYARGKLLKSK
N
>Mature_401_residues
MYDDFINNENILLIINAIKKFQGDIRLVGGCVRDSLLKRQTIDIDFATTLLPNQTINALTAAHIKAIPTGIKHGTITALV
NNTAYEITTLRSDISCDGRHAEVKFTNNWQQDASRRDFTFNALYCDEKGIVYDYFSGIQDLEKKHLNFIGDPEIRIQEDY
LRILRAFRFYASICSQNKLSDEIVHSCTKYSSYINNLSRERIRDEFFKLLLCPNLSNTLKIMQKCHVLDKIIPFEVIPDI
MSSETLSNTDPLTKLAALLRTNNNNHSLDKIKASLCLSNYSQKTLVSLLNNNLELPLSTTAQHKHINKLGKEIYCNLLRI
IHAELNLNYHDLMQYIEYADQLIIPEFPISGKDLLNIGYQPGKNLGITLEKIKDLWENSSYQLTKTQLLDYARGKLLKSK
N

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=388, Percent_Identity=33.7628865979381, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI87081691, Length=237, Percent_Identity=29.1139240506329, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=392, Percent_Identity=30.6122448979592, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281360112, Length=389, Percent_Identity=33.1619537275064, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI21357337, Length=389, Percent_Identity=33.1619537275064, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45955; Mature: 45955

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDDFINNENILLIINAIKKFQGDIRLVGGCVRDSLLKRQTIDIDFATTLLPNQTINALT
CCCCCCCCCCEEEEEHHHHHHCCCCEEEHHHHHHHHHHHHCCCEEHHHHHCCCCHHHHHH
AAHIKAIPTGIKHGTITALVNNTAYEITTLRSDISCDGRHAEVKFTNNWQQDASRRDFTF
HHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCEE
NALYCDEKGIVYDYFSGIQDLEKKHLNFIGDPEIRIQEDYLRILRAFRFYASICSQNKLS
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCH
DEIVHSCTKYSSYINNLSRERIRDEFFKLLLCPNLSNTLKIMQKCHVLDKIIPFEVIPDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
MSSETLSNTDPLTKLAALLRTNNNNHSLDKIKASLCLSNYSQKTLVSLLNNNLELPLSTT
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
AQHKHINKLGKEIYCNLLRIIHAELNLNYHDLMQYIEYADQLIIPEFPISGKDLLNIGYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEECCCCCCCCCHHHCCCCC
PGKNLGITLEKIKDLWENSSYQLTKTQLLDYARGKLLKSKN
CCCCCCEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYDDFINNENILLIINAIKKFQGDIRLVGGCVRDSLLKRQTIDIDFATTLLPNQTINALT
CCCCCCCCCCEEEEEHHHHHHCCCCEEEHHHHHHHHHHHHCCCEEHHHHHCCCCHHHHHH
AAHIKAIPTGIKHGTITALVNNTAYEITTLRSDISCDGRHAEVKFTNNWQQDASRRDFTF
HHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEEEECCCHHHCCCCCCCEE
NALYCDEKGIVYDYFSGIQDLEKKHLNFIGDPEIRIQEDYLRILRAFRFYASICSQNKLS
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCH
DEIVHSCTKYSSYINNLSRERIRDEFFKLLLCPNLSNTLKIMQKCHVLDKIIPFEVIPDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
MSSETLSNTDPLTKLAALLRTNNNNHSLDKIKASLCLSNYSQKTLVSLLNNNLELPLSTT
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
AQHKHINKLGKEIYCNLLRIIHAELNLNYHDLMQYIEYADQLIIPEFPISGKDLLNIGYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEECCCCCCCCCHHHCCCCC
PGKNLGITLEKIKDLWENSSYQLTKTQLLDYARGKLLKSKN
CCCCCCEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA