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The map label for this gene is tpiA

Identifier: 88658414

GI number: 88658414

Start: 650242

End: 650964

Strand: Reverse

Name: tpiA

Synonym: ECH_0646

Alternate gene names: 88658414

Gene position: 650964-650242 (Counterclockwise)

Preceding gene: 88657729

Following gene: 88658051

Centisome position: 55.34

GC content: 32.23

Gene sequence:

>723_bases
ATGTCATTGCTCATCGTTGCTAATTGGAAGATGTATGGTGACTTTCTTACTTTTTCCTCTTTTACAAAAGAATTAAGTGC
TAATTTAGTTAATGTAAAGGCTGATGTAGAAGTGGTATTGTGTCCACCTTTTATTGCATGTTCTAAGATAGTTGATTGTG
CTCCCAATATTAAACTTGGTGCACAGAACTGTTTTTATGAATCTGAAGGGAAATATACTGGGGAAGTTAGTGCTAAAATG
TTGTATAGTTGTGGTTGCAGTTATGTAATAGTAGGTCACTATGAGAGAAGAAGCATATTTTATGAATCTGATTATTGTGT
ACAGTTGAAGGCAAAATCTGCAATAGATGCAGGGTTAATTCCAATTATTTGTATTGGGGAAACTTTGTTAGATAGAGAAA
ATGGTATGTTAAAAAATGCTTTATTAGATCAGTGTTATAATTCTTTCCCTAAACATGGTGAGTTTGTTATAGCTTATGAG
CCTGTATGGGCAATAGGAAGTAATACTATTCCTTCTATTGATATGATAACTGAATCTTTAGATATTATAAGGTCGTATGA
TAGTAAATCTAATATTATATATGGTGGTGCAGTGAATCAGAGTAACATAAAGGATGTTATTGCTATAAATCAATTGTCTG
GTGTATTGGTTGGTAGTGCCAGTTTAAAGGTAAGTAGTTTTTGCGATATAATATATGGTGCTGTAAATGTGAGGCAAAAT
TAA

Upstream 100 bases:

>100_bases
GTTCCAGCCGGCTTAGCTCAACTGGGAGAGCATCTGATTTGTAATCAGAGGGTTATGAGTTCAAGTCTTATAGCCGGCAC
ATTTAATTTTAGTAATTTTT

Downstream 100 bases:

>100_bases
TGAAAAAAGTGTTAATTGTGTATGTATTTATTATTAGTGTACTTTTTAATAATGCAATTGCCTCGTCAGATGATAGTTTA
AATAGAGAATATATTTTAAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSLLIVANWKMYGDFLTFSSFTKELSANLVNVKADVEVVLCPPFIACSKIVDCAPNIKLGAQNCFYESEGKYTGEVSAKM
LYSCGCSYVIVGHYERRSIFYESDYCVQLKAKSAIDAGLIPIICIGETLLDRENGMLKNALLDQCYNSFPKHGEFVIAYE
PVWAIGSNTIPSIDMITESLDIIRSYDSKSNIIYGGAVNQSNIKDVIAINQLSGVLVGSASLKVSSFCDIIYGAVNVRQN

Sequences:

>Translated_240_residues
MSLLIVANWKMYGDFLTFSSFTKELSANLVNVKADVEVVLCPPFIACSKIVDCAPNIKLGAQNCFYESEGKYTGEVSAKM
LYSCGCSYVIVGHYERRSIFYESDYCVQLKAKSAIDAGLIPIICIGETLLDRENGMLKNALLDQCYNSFPKHGEFVIAYE
PVWAIGSNTIPSIDMITESLDIIRSYDSKSNIIYGGAVNQSNIKDVIAINQLSGVLVGSASLKVSSFCDIIYGAVNVRQN
>Mature_239_residues
SLLIVANWKMYGDFLTFSSFTKELSANLVNVKADVEVVLCPPFIACSKIVDCAPNIKLGAQNCFYESEGKYTGEVSAKML
YSCGCSYVIVGHYERRSIFYESDYCVQLKAKSAIDAGLIPIICIGETLLDRENGMLKNALLDQCYNSFPKHGEFVIAYEP
VWAIGSNTIPSIDMITESLDIIRSYDSKSNIIYGGAVNQSNIKDVIAINQLSGVLVGSASLKVSSFCDIIYGAVNVRQN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=34.9593495934959, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=34.9593495934959, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=34.6613545816733, Blast_Score=134, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=35.2697095435685, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320255, Length=244, Percent_Identity=32.7868852459016, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=36.5853658536585, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=36.5853658536585, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=36.4372469635627, Blast_Score=137, Evalue=6e-33,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_EHRCR (Q2GGH7)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507456.1
- ProteinModelPortal:   Q2GGH7
- SMR:   Q2GGH7
- STRING:   Q2GGH7
- GeneID:   3927860
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0646
- TIGR:   ECH_0646
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK14565
- BioCyc:   ECHA205920:ECH_0646-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26331; Mature: 26199

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 160-160 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLIVANWKMYGDFLTFSSFTKELSANLVNVKADVEVVLCPPFIACSKIVDCAPNIKLG
CEEEEEECCEEECHHHHHHHHHHHHHCCEEEEEECEEEEECCCHHHHHHHHHCCCCCEEC
AQNCFYESEGKYTGEVSAKMLYSCGCSYVIVGHYERRSIFYESDYCVQLKAKSAIDAGLI
CCCCEECCCCCEECCHHHHHHHHCCCCEEEEEEEECCEEEECCCEEEEEEECCCCCCCCE
PIICIGETLLDRENGMLKNALLDQCYNSFPKHGEFVIAYEPVWAIGSNTIPSIDMITESL
EEEEECHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHH
DIIRSYDSKSNIIYGGAVNQSNIKDVIAINQLSGVLVGSASLKVSSFCDIIYGAVNVRQN
HHHHCCCCCCCEEEECCCCCCCCHHEEEEECCCCEEEECCCEEHHHHHHHHHHHEEECCC
>Mature Secondary Structure 
SLLIVANWKMYGDFLTFSSFTKELSANLVNVKADVEVVLCPPFIACSKIVDCAPNIKLG
EEEEEECCEEECHHHHHHHHHHHHHCCEEEEEECEEEEECCCHHHHHHHHHCCCCCEEC
AQNCFYESEGKYTGEVSAKMLYSCGCSYVIVGHYERRSIFYESDYCVQLKAKSAIDAGLI
CCCCEECCCCCEECCHHHHHHHHCCCCEEEEEEEECCEEEECCCEEEEEEECCCCCCCCE
PIICIGETLLDRENGMLKNALLDQCYNSFPKHGEFVIAYEPVWAIGSNTIPSIDMITESL
EEEEECHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHH
DIIRSYDSKSNIIYGGAVNQSNIKDVIAINQLSGVLVGSASLKVSSFCDIIYGAVNVRQN
HHHHCCCCCCCEEEECCCCCCCCHHEEEEECCCCEEEECCCEEHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA