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The map label for this gene is rnd [H]

Identifier: 88658362

GI number: 88658362

Start: 286446

End: 287609

Strand: Direct

Name: rnd [H]

Synonym: ECH_0300

Alternate gene names: 88658362

Gene position: 286446-287609 (Clockwise)

Preceding gene: 88658105

Following gene: 88658425

Centisome position: 24.35

GC content: 28.26

Gene sequence:

>1164_bases
ATGTTAATTGATACTGTCAAAGAATTAGAGCGTTTGTGTAATCAGCTGCTTGTATTGCAACCTAAATTTATTGCAGTTGA
TACTGAATTTGTAAGAAGTAGTAGTGAATATTATCCTAAATTATGTTTAATTCAAATTGCATATGGTAAAGAACAGTTTG
TTATTGATGTTTTAGCGAAAGATATGGATTTATCAGTTTTAGGACATATTTTTTATAATGAAAGTATAATAAAAGTTTTT
CATGATTGTAGACAAGATATAGATGCATTGTTAACAAAATTTCCAGAAATTCCTAGCCCTATATTTGATACTCAAATTGC
TGCCATGTTTTGTTGTTGTTATGATAATGCAGTTGGATATTCTAAGTTAGTAGAGCAATTTTTAGGTATTTCATTAGATA
AGCTGAGCTTGAAAAGATCTAATTGGGCGCTTCGACCATTATCTGCTGATAAAATCCAGTATGCACTTAACGATGTTATA
TATTTGTATGAGCTTTATCAAATATTATATAACAATTTATTAGATGCTGGAAAGTTACCTTGGTTTTTGGAAGAGATGAA
TAATATAGTGTTACAGGAAGTTAACTGTAATGATGTTTGTGATATTCCTGATTTTGGTTCTGATATGACAAAAGAGGAGA
TTATTGTTGTAAGATCTGTAATTGAATGGCGAGAAAGATTAGCAAAGTTTTTTAATCTTAATCGCGAATTTATATTAAAA
AACAAGGTAATGCTATCTTTAGTTAGAGATTTTATAAAAAAAAGTGATATTAGTACTTTAGGTAAGTATATAAAGAAAGA
ATATTTATCATGTGATGCAGTGTCTGATCTTTTACAAGTTATTGATAATAATCAATCTGTTAATATTGATGAGTTACATT
ATGTAAGTGTTAATAGTTATAATAAGTCTGTTTTAAATGTGCTGCTTATATTGTTATATAATATGTGTTACAAAAATAAT
ATCTCACAGAAGTTAGTTGCATCTAAAGGTGGCCTAATTAGGTTTCTTAACGGTAAGTTTAGTTATGTAATGAAAGGTTG
GAGATATGAATTTTTTGGATGCGAAGTTGAGAAATTTATGCAAGGAAAATCTAAGATTATAGTCTCAGCACAAGTACTGG
ATAATGATTGTATGGATATTGTAACTACTTTGACGGAAATGTAG

Upstream 100 bases:

>100_bases
TATTTTATTGGTTATTTTTAATAGATATAAAAAGATTTTATATTAAGTATTTTTGCACTGAAATTATAAAGGTTTAAATT
GTGAAGAAGAAAATAAGTAT

Downstream 100 bases:

>100_bases
ACTTGTTGTAAAGTAAGAACTCTGATAGATATTATAGGATACTGTAATTACTGGAGAAACGTATGTTAGTGCAGTAATAG
CATAAAAAAATGTATGTGTT

Product: putative ribonuclease D

Products: NA

Alternate protein names: RNase D [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLIDTVKELERLCNQLLVLQPKFIAVDTEFVRSSSEYYPKLCLIQIAYGKEQFVIDVLAKDMDLSVLGHIFYNESIIKVF
HDCRQDIDALLTKFPEIPSPIFDTQIAAMFCCCYDNAVGYSKLVEQFLGISLDKLSLKRSNWALRPLSADKIQYALNDVI
YLYELYQILYNNLLDAGKLPWFLEEMNNIVLQEVNCNDVCDIPDFGSDMTKEEIIVVRSVIEWRERLAKFFNLNREFILK
NKVMLSLVRDFIKKSDISTLGKYIKKEYLSCDAVSDLLQVIDNNQSVNIDELHYVSVNSYNKSVLNVLLILLYNMCYKNN
ISQKLVASKGGLIRFLNGKFSYVMKGWRYEFFGCEVEKFMQGKSKIIVSAQVLDNDCMDIVTTLTEM

Sequences:

>Translated_387_residues
MLIDTVKELERLCNQLLVLQPKFIAVDTEFVRSSSEYYPKLCLIQIAYGKEQFVIDVLAKDMDLSVLGHIFYNESIIKVF
HDCRQDIDALLTKFPEIPSPIFDTQIAAMFCCCYDNAVGYSKLVEQFLGISLDKLSLKRSNWALRPLSADKIQYALNDVI
YLYELYQILYNNLLDAGKLPWFLEEMNNIVLQEVNCNDVCDIPDFGSDMTKEEIIVVRSVIEWRERLAKFFNLNREFILK
NKVMLSLVRDFIKKSDISTLGKYIKKEYLSCDAVSDLLQVIDNNQSVNIDELHYVSVNSYNKSVLNVLLILLYNMCYKNN
ISQKLVASKGGLIRFLNGKFSYVMKGWRYEFFGCEVEKFMQGKSKIIVSAQVLDNDCMDIVTTLTEM
>Mature_387_residues
MLIDTVKELERLCNQLLVLQPKFIAVDTEFVRSSSEYYPKLCLIQIAYGKEQFVIDVLAKDMDLSVLGHIFYNESIIKVF
HDCRQDIDALLTKFPEIPSPIFDTQIAAMFCCCYDNAVGYSKLVEQFLGISLDKLSLKRSNWALRPLSADKIQYALNDVI
YLYELYQILYNNLLDAGKLPWFLEEMNNIVLQEVNCNDVCDIPDFGSDMTKEEIIVVRSVIEWRERLAKFFNLNREFILK
NKVMLSLVRDFIKKSDISTLGKYIKKEYLSCDAVSDLLQVIDNNQSVNIDELHYVSVNSYNKSVLNVLLILLYNMCYKNN
ISQKLVASKGGLIRFLNGKFSYVMKGWRYEFFGCEVEKFMQGKSKIIVSAQVLDNDCMDIVTTLTEM

Specific function: Cleaves multimeric tRNA precursor at the spacer region. Contribute to the 3' maturation of several stable RNAs [H]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=385, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17531757, Length=259, Percent_Identity=29.3436293436293, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324574, Length=264, Percent_Identity=27.6515151515151, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI161078302, Length=270, Percent_Identity=25.1851851851852, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR002121
- InterPro:   IPR010997
- InterPro:   IPR006292
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00570 HRDC [H]

EC number: =3.1.13.5 [H]

Molecular weight: Translated: 44768; Mature: 44768

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDTVKELERLCNQLLVLQPKFIAVDTEFVRSSSEYYPKLCLIQIAYGKEQFVIDVLAK
CCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHC
DMDLSVLGHIFYNESIIKVFHDCRQDIDALLTKFPEIPSPIFDTQIAAMFCCCYDNAVGY
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCH
SKLVEQFLGISLDKLSLKRSNWALRPLSADKIQYALNDVIYLYELYQILYNNLLDAGKLP
HHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WFLEEMNNIVLQEVNCNDVCDIPDFGSDMTKEEIIVVRSVIEWRERLAKFFNLNREFILK
HHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NKVMLSLVRDFIKKSDISTLGKYIKKEYLSCDAVSDLLQVIDNNQSVNIDELHYVSVNSY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
NKSVLNVLLILLYNMCYKNNISQKLVASKGGLIRFLNGKFSYVMKGWRYEFFGCEVEKFM
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCHHHHHHCCCEEEECCCHHHHH
QGKSKIIVSAQVLDNDCMDIVTTLTEM
CCCCEEEEEHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MLIDTVKELERLCNQLLVLQPKFIAVDTEFVRSSSEYYPKLCLIQIAYGKEQFVIDVLAK
CCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHC
DMDLSVLGHIFYNESIIKVFHDCRQDIDALLTKFPEIPSPIFDTQIAAMFCCCYDNAVGY
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCH
SKLVEQFLGISLDKLSLKRSNWALRPLSADKIQYALNDVIYLYELYQILYNNLLDAGKLP
HHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WFLEEMNNIVLQEVNCNDVCDIPDFGSDMTKEEIIVVRSVIEWRERLAKFFNLNREFILK
HHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NKVMLSLVRDFIKKSDISTLGKYIKKEYLSCDAVSDLLQVIDNNQSVNIDELHYVSVNSY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
NKSVLNVLLILLYNMCYKNNISQKLVASKGGLIRFLNGKFSYVMKGWRYEFFGCEVEKFM
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCHHHHHHCCCEEEECCCHHHHH
QGKSKIIVSAQVLDNDCMDIVTTLTEM
CCCCEEEEEHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3041371; 9097040; 9278503; 8107670 [H]