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The map label for this gene is atoS [C]

Identifier: 88658105

GI number: 88658105

Start: 284058

End: 286202

Strand: Direct

Name: atoS [C]

Synonym: ECH_0299

Alternate gene names: 88658105

Gene position: 284058-286202 (Clockwise)

Preceding gene: 88658217

Following gene: 88658362

Centisome position: 24.15

GC content: 31.0

Gene sequence:

>2145_bases
ATGAAAGGCTGGTTTATAAAGGTATACAGTAATATAAGTAGATTTCAGTTTTTTAAAATCGTTGCTTCGTTCTTAGCAAT
ATCTGTTTTCTTGTTTTTGATTATAACATACTGTGTTGTTTTTCATCATGATGATCCCCTTGGGCCTGATCATAGTAGGG
CTATAAAGTTAGTATTCTTTGATTTGATATTATTTCTACTGTTGATTGCTAGTGTATCTCATAAGTTGATTAATATGTGG
ATACGAAGAAAAAGGGGACATTTAGGTTCTCATTTGCAAACAAAGATTATTCTAATGTTTTCAGTAGTAGCTGTTATTCC
AACAATTGTGATTTCAGGTTTTTCTACTTTGTTTTTTAATTATAGTATTCAAGCTTGGTTTAATAAAAGGGTTGAAGCTG
TAATGCATGAGTCTATCCAAGTGGCAGAGGCTTATTTAAGGGAGCATAAGAGAAATATTCGTTCTGATATTTTAGCTATT
TCACATTATATATCTGATCACAAGATAATATTAAATTATGATACAGCAGCATTGCAAAATGTTGTGCAATCTAGGGCAGA
TTTATTAGGATTGGCTGAAATAATAATTTTTGAACCAAGTAGGATAGTAGCAAGTAATAAGTTTAGTTTTGTATTAAATT
TTGATATGGTCCCATGGAATGACTTAGATAGAGTGGATAATGATAGGATTGTTATTATAATAAAAGAAAATATGATACGT
GCTTTTCTTTTACTTGATAGATTCTCATCTACTTATATCATGGTAAGTCGTATTATAGATAGAAAAGTTATTTCTCATTT
ATCAGCAACGAAAGGAGCAGTTAAATCATATAGAGATCTGCAATCACAGATATCATCACTACAAATACAGTTTTCTATGA
TATTTATTTTAATATCGTTATTGTTATTATTGACTGCTATTTGGTACGGAATTAATTTTTCTGGTGACATAGTTCGTCCA
CTGTTGGATTTATTCTACGCTACACGTAAAGTTCAAAAGGGTGATTTATCTTTCAAGATAGAAGAAGGACGAGTTGGAGA
AGAAATGTCTACGCTTGCACGTGCATTCAATCAAATGACGTCACAGCTTAGTAGCCAACGTTCACAATTGATAAAACTTT
ATCAAGATATGAATGAACGAAGAGAATTTATAGAAGCTGTTTTGTCAGGTGTTTCTTCTGGAATAATAGCAGTAAACTGT
GCTGGTATAATTACTTTAATGAATGATAAAGCAAAGGAATTATTAGCTCCTAATGATATCCTACATGCAGAACTTGATGA
TGTATTTCCTGAAATATCTGAATTGATTAAAAGCTCTAAAATGCATGATGAAATTACTGTTTTGAGAAATAGAAAGTCTT
TCACTTTATCTGTGAGAATAAAATTGCTTGGTAGTGTTAATAAAGGATATATTATTACATTTGATGATATTTCAAGTTTA
GTTGATGCACAGAGATCAGCTGCATGGTCTGATGTTGCAAGAAGAATAGCTCATGAAATAAAAAATCCTATTACTCCAAT
ATATCTTGCTGCTGAAAGATTAAGTAGCAAGTATCAGCATGAAATTATTTCTGATAAGGAAAGTTTTGGAAGATATATTG
ATACAATAATTCGCCATGTGACGAGTATTAGGTGTATTGTTGATGAGTTTGCAAAATTTGCGAAAATGTCGGATCCTGTG
TTAATTAAACATGATATTTGTAGTGTAGTAAAAGAGTTGGCGTTTTCCGGGCAGTTTAGTCGTGGTGTTGTGCATTATGA
GTTAGATATACCAAGTTATCCAATTTTTGTTGTATTAGATAAAAATCAGATAAATCATGTATTTATTAACTTGTTCAAGA
ATGCATGTGAATCAATTGATATGAAGTCTAATAGTATACAGGGACTGATAAAAATTAGTGTAGTTGATGATAGAGATTGT
GTTAATGTGCAAATTCAGGATAATGGTGTAGGATTTCCAGAAGAACTTATGGAAAAGCTTACAGAACCTTATGTTACAAC
TCGTGTCCAAGGAACAGGATTAGGTTTATCCATAGTAAAAAAGATACTAGATGAACATAATGCAACAATAAGTTTCTATA
ATATAGAAGATGGTGGTCTGGTTAAGTTGACATTTGTAAAATGTCAGGATTGTGAAAATATTTAA

Upstream 100 bases:

>100_bases
TTTTAGATGTAGGTTCTTTTAGATTATTATCGGTTATGTAAGCATAAGGGCTGATCTTTTACATTTGTTATGAATAATTT
CTTCAGCATGTGATATCCTA

Downstream 100 bases:

>100_bases
TGTAAATTTATAATATCCTATTATTAGCAGTTTTGTTTATCATTAAAGATACGATTAATATTAATTTGCTCAGGTCAATT
TTAGATAGTTTTCAAGAGAA

Product: putative nitrogen regulation protein NtrY

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKGWFIKVYSNISRFQFFKIVASFLAISVFLFLIITYCVVFHHDDPLGPDHSRAIKLVFFDLILFLLLIASVSHKLINMW
IRRKRGHLGSHLQTKIILMFSVVAVIPTIVISGFSTLFFNYSIQAWFNKRVEAVMHESIQVAEAYLREHKRNIRSDILAI
SHYISDHKIILNYDTAALQNVVQSRADLLGLAEIIIFEPSRIVASNKFSFVLNFDMVPWNDLDRVDNDRIVIIIKENMIR
AFLLLDRFSSTYIMVSRIIDRKVISHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISLLLLLTAIWYGINFSGDIVRP
LLDLFYATRKVQKGDLSFKIEEGRVGEEMSTLARAFNQMTSQLSSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNC
AGIITLMNDKAKELLAPNDILHAELDDVFPEISELIKSSKMHDEITVLRNRKSFTLSVRIKLLGSVNKGYIITFDDISSL
VDAQRSAAWSDVARRIAHEIKNPITPIYLAAERLSSKYQHEIISDKESFGRYIDTIIRHVTSIRCIVDEFAKFAKMSDPV
LIKHDICSVVKELAFSGQFSRGVVHYELDIPSYPIFVVLDKNQINHVFINLFKNACESIDMKSNSIQGLIKISVVDDRDC
VNVQIQDNGVGFPEELMEKLTEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEDGGLVKLTFVKCQDCENI

Sequences:

>Translated_714_residues
MKGWFIKVYSNISRFQFFKIVASFLAISVFLFLIITYCVVFHHDDPLGPDHSRAIKLVFFDLILFLLLIASVSHKLINMW
IRRKRGHLGSHLQTKIILMFSVVAVIPTIVISGFSTLFFNYSIQAWFNKRVEAVMHESIQVAEAYLREHKRNIRSDILAI
SHYISDHKIILNYDTAALQNVVQSRADLLGLAEIIIFEPSRIVASNKFSFVLNFDMVPWNDLDRVDNDRIVIIIKENMIR
AFLLLDRFSSTYIMVSRIIDRKVISHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISLLLLLTAIWYGINFSGDIVRP
LLDLFYATRKVQKGDLSFKIEEGRVGEEMSTLARAFNQMTSQLSSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNC
AGIITLMNDKAKELLAPNDILHAELDDVFPEISELIKSSKMHDEITVLRNRKSFTLSVRIKLLGSVNKGYIITFDDISSL
VDAQRSAAWSDVARRIAHEIKNPITPIYLAAERLSSKYQHEIISDKESFGRYIDTIIRHVTSIRCIVDEFAKFAKMSDPV
LIKHDICSVVKELAFSGQFSRGVVHYELDIPSYPIFVVLDKNQINHVFINLFKNACESIDMKSNSIQGLIKISVVDDRDC
VNVQIQDNGVGFPEELMEKLTEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEDGGLVKLTFVKCQDCENI
>Mature_714_residues
MKGWFIKVYSNISRFQFFKIVASFLAISVFLFLIITYCVVFHHDDPLGPDHSRAIKLVFFDLILFLLLIASVSHKLINMW
IRRKRGHLGSHLQTKIILMFSVVAVIPTIVISGFSTLFFNYSIQAWFNKRVEAVMHESIQVAEAYLREHKRNIRSDILAI
SHYISDHKIILNYDTAALQNVVQSRADLLGLAEIIIFEPSRIVASNKFSFVLNFDMVPWNDLDRVDNDRIVIIIKENMIR
AFLLLDRFSSTYIMVSRIIDRKVISHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISLLLLLTAIWYGINFSGDIVRP
LLDLFYATRKVQKGDLSFKIEEGRVGEEMSTLARAFNQMTSQLSSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNC
AGIITLMNDKAKELLAPNDILHAELDDVFPEISELIKSSKMHDEITVLRNRKSFTLSVRIKLLGSVNKGYIITFDDISSL
VDAQRSAAWSDVARRIAHEIKNPITPIYLAAERLSSKYQHEIISDKESFGRYIDTIIRHVTSIRCIVDEFAKFAKMSDPV
LIKHDICSVVKELAFSGQFSRGVVHYELDIPSYPIFVVLDKNQINHVFINLFKNACESIDMKSNSIQGLIKISVVDDRDC
VNVQIQDNGVGFPEELMEKLTEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEDGGLVKLTFVKCQDCENI

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in nitrogen level control. Probably activates ntrX by phosphorylation [H]

COG id: COG5000

COG function: function code T; Signal transduction histidine kinase involved in nitrogen fixation and metabolism regulation

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=380, Percent_Identity=23.4210526315789, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1790300, Length=229, Percent_Identity=28.3842794759825, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=24.1860465116279, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=213, Percent_Identity=25.8215962441315, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017232 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81334; Mature: 81334

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGWFIKVYSNISRFQFFKIVASFLAISVFLFLIITYCVVFHHDDPLGPDHSRAIKLVFF
CCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DLILFLLLIASVSHKLINMWIRRKRGHLGSHLQTKIILMFSVVAVIPTIVISGFSTLFFN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSIQAWFNKRVEAVMHESIQVAEAYLREHKRNIRSDILAISHYISDHKIILNYDTAALQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
VVQSRADLLGLAEIIIFEPSRIVASNKFSFVLNFDMVPWNDLDRVDNDRIVIIIKENMIR
HHHHHHHHHHHHHHEEECCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHH
AFLLLDRFSSTYIMVSRIIDRKVISHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLTAIWYGINFSGDIVRPLLDLFYATRKVQKGDLSFKIEEGRVGEEMSTLARAFNQMT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SQLSSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNCAGIITLMNDKAKELLAPNDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHHCCCCHH
LHAELDDVFPEISELIKSSKMHDEITVLRNRKSFTLSVRIKLLGSVNKGYIITFDDISSL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHH
VDAQRSAAWSDVARRIAHEIKNPITPIYLAAERLSSKYQHEIISDKESFGRYIDTIIRHV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TSIRCIVDEFAKFAKMSDPVLIKHDICSVVKELAFSGQFSRGVVHYELDIPSYPIFVVLD
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEC
KNQINHVFINLFKNACESIDMKSNSIQGLIKISVVDDRDCVNVQIQDNGVGFPEELMEKL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHH
TEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEDGGLVKLTFVKCQDCENI
CCCHHEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKGWFIKVYSNISRFQFFKIVASFLAISVFLFLIITYCVVFHHDDPLGPDHSRAIKLVFF
CCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DLILFLLLIASVSHKLINMWIRRKRGHLGSHLQTKIILMFSVVAVIPTIVISGFSTLFFN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSIQAWFNKRVEAVMHESIQVAEAYLREHKRNIRSDILAISHYISDHKIILNYDTAALQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
VVQSRADLLGLAEIIIFEPSRIVASNKFSFVLNFDMVPWNDLDRVDNDRIVIIIKENMIR
HHHHHHHHHHHHHHEEECCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHH
AFLLLDRFSSTYIMVSRIIDRKVISHLSATKGAVKSYRDLQSQISSLQIQFSMIFILISL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLTAIWYGINFSGDIVRPLLDLFYATRKVQKGDLSFKIEEGRVGEEMSTLARAFNQMT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
SQLSSQRSQLIKLYQDMNERREFIEAVLSGVSSGIIAVNCAGIITLMNDKAKELLAPNDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHHCCCCHH
LHAELDDVFPEISELIKSSKMHDEITVLRNRKSFTLSVRIKLLGSVNKGYIITFDDISSL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEEECCCCCEEEEEHHHHHH
VDAQRSAAWSDVARRIAHEIKNPITPIYLAAERLSSKYQHEIISDKESFGRYIDTIIRHV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TSIRCIVDEFAKFAKMSDPVLIKHDICSVVKELAFSGQFSRGVVHYELDIPSYPIFVVLD
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEC
KNQINHVFINLFKNACESIDMKSNSIQGLIKISVVDDRDCVNVQIQDNGVGFPEELMEKL
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHH
TEPYVTTRVQGTGLGLSIVKKILDEHNATISFYNIEDGGLVKLTFVKCQDCENI
CCCHHEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]