Definition Ehrlichia chaffeensis str. Arkansas, complete genome.
Accession NC_007799
Length 1,176,248

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The map label for this gene is pnp

Identifier: 88658296

GI number: 88658296

Start: 735568

End: 737958

Strand: Reverse

Name: pnp

Synonym: ECH_0726

Alternate gene names: 88658296

Gene position: 737958-735568 (Counterclockwise)

Preceding gene: 88657975

Following gene: 88657758

Centisome position: 62.74

GC content: 35.34

Gene sequence:

>2391_bases
ATGTTTAATTTGATAAGAAGATCTGCAGAATGGGGAGGGAAAACCTTAGTTTTAGAGAGTGGAAAGATAGCAAGACAAGC
TAGTGGTGCCGTTATGGTTAGTTATGCCGGTACTACAGTTTTGGCAACAGTAGTAACAGGTAAAACTAAGGAACCGGTAG
ATTTTCTACCATTAACTGTACAGTTTGTTGCGAAGAGTTATGCTGTTGGTAAGATTCCTGGTGGTTTTTTGAAGAGAGAA
GGTAAACCATCTGATAGGGAGACGTTGATTTCTCGTTTAATTGATAGGAGTATAAGACCTTTATTTCCTGCTGGATTTTA
TGATGAGATTAGTATAGTGTGTAATTTGCTTTCATATGATACTGTAACACCTCCAGAGGTTACAGCATTAGTTGGAGCAA
CTGCAGCTTTATCTATATCTGGTGTTCCTTTTAATGGTTTGGTTGTTGGTGCAAGGGTTGGCTATTTACCATCTGAAGGT
AAGTATTTATTAAATGCATCTGCTGATGAGATGCTCTGTAGTTCTTTAGATTTATTTTTATCAGGTAATGAAGATTCTGT
TTTGATGGTTGAATCCGAGGCATCTGAGTTATCTGAATCTCAGATGTTAGGTGCAATAACTTTTGGGCATCAACATTGTC
AAGAGGTTATTAATTTAATTAAAGAATTTAGTCATGAAAGTGGTCAAACACCTATTGACTTCATACCCCATGATATTAGT
TCACTTGTGAGTGATATAGAGTCCTCTTATAAAGAGGATTTTAGTTTAGCATACTCTAACACTATAAAGAAAGAAAGGGT
ATTAAAATTGGAGGAATTGAGGGGTAAAGTATTGTCTGAAGTGGCAGATAAATATAGTGCTGGTGATGTGGAGTGTAGTG
ATCAAGATATAGTTACTGCATTAAAAACCTTTGAAAGATCGTTAGTGAGATCCAAAATTATTGATACCTCTTCTAGAATA
GATGGCCGTGCATTTGATGAGATACGTGATATAGAAATTGAAGTGGATGTATTGCCTAAAGCGCATGGTTCTGCTTTATT
TACTCGAGGGAACACGCAAGCATTGGTTGTTACTGCATTAGGTACACCTCAAGATGAGCAGATAGTTGATGATTTAGATG
GTGATAGAAGAGAAAATTTTTTGTTACATTATAATTTTCCTCCGTATGCAGTTGGTGAGTCTGCAGCTTTACGTGCTCCT
GGTAGAAGAGAAATAGGTCATGGTAAGCTTGCGTGGAGGGCAATTCGTTATGTATTACCTGAAAAGTCAGATTTTCCATA
TACTATTAGAGTAGTTTCTGAAATTACAGAGTCAGATGGTTCTTCTTCAATGGCTACAGTTTGTGGTGCGTCTTTGGCTT
TAATGGATACAGGTGTGCCAATTAAATCTCCTGTTGCTGGAATTGCAATGGGGCTCATTAAAGAAGATGATAGGTTTATA
ATACTTTCAGATATATTGGGTGATGAAGATCATCTTGGTGATATGGATTTTAAAGTAGCTGGTACTGCTGAAGGTGTTAC
TGCTTTACAAATGGATATGAAAATATCGGGTATTGACATTGATATAATTGAAAAAGCATTACTTCAAGCTAAAGATGGTA
GAATGCATATTTTAAGTAAAATGAATGCAGTGATACAAGAATCTCGCAATCGTATAAAAAATCATGCTCCTAGGATAGAA
TCTATTTTTATAAATAAGGATAAGATTCGTAATGTAATAGGAAGTGGAGGAAAAAATATACGTGATATATGTGAAAAAAC
AGGTGCGAAAATTGAAATAATCCAAGATGGCACTGTTATGATATATGCTGTTAACAATGAGGCAGTAGAATATGCTAAAA
GCATGATAATGGATATTGTAACTGAACCAGAGATAGGTAAGGTTTTTGAAGGTACTGTTGTTGAAATTATGAAGTTTGGA
GCTTTTGTCAGCTTCTTGGGTGGTAAGAAAGGATTAGTACATATTAGTGAAATAAGGAATGAACATATTAGTTCAGTAGG
TAGTGTTATTTCTTTGAATGATAAAGTAAAAGTGTTAGTAATTGGTATAGATCGTGAACATATCCAATTATCTATGCGCA
GAGTAGATCAGGAAAGTGGAGAACCTATAGATGGTGAGCTTTATAATATAAGGAAAAATAGTTTTTCTGATGATTCATGT
GGTAGTACTGGTGGTAGTAGTTTTAAGGAGAGCTATAACCCAAATAGTAGACATGGTAGTCATGAAAAAAAACGTAGTGG
TGGTAGTAGTAGATCATCACGTCGTAACAGTAATGGACCTAACTATTATAGAGAAGATTTACCATCGTCAAACGGATTTG
GGAATAATAATCGCTCATTCAGTAATAGTAGAAATGGTCATGATGTTCCTAGGAAGCCTAGGTTTTTTTAA

Upstream 100 bases:

>100_bases
GAAGAAATATGGGGATAGTGAATATTTAGCATTGATAAAACGATTAGGTATCAGGGATATTTTTCACTGATTTAATTTAT
AGAAAAGTAGGAAATAATGT

Downstream 100 bases:

>100_bases
TTTAGATTGCTACGTAGAAGATCTCTATGCTTATTTTTGATCGTGCCCTTGTAAGGTTTTATAGGGATAGGCTATCTTGT
AATAAGGATAATGACTTTAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MFNLIRRSAEWGGKTLVLESGKIARQASGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTVQFVAKSYAVGKIPGGFLKRE
GKPSDRETLISRLIDRSIRPLFPAGFYDEISIVCNLLSYDTVTPPEVTALVGATAALSISGVPFNGLVVGARVGYLPSEG
KYLLNASADEMLCSSLDLFLSGNEDSVLMVESEASELSESQMLGAITFGHQHCQEVINLIKEFSHESGQTPIDFIPHDIS
SLVSDIESSYKEDFSLAYSNTIKKERVLKLEELRGKVLSEVADKYSAGDVECSDQDIVTALKTFERSLVRSKIIDTSSRI
DGRAFDEIRDIEIEVDVLPKAHGSALFTRGNTQALVVTALGTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAP
GRREIGHGKLAWRAIRYVLPEKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEDDRFI
ILSDILGDEDHLGDMDFKVAGTAEGVTALQMDMKISGIDIDIIEKALLQAKDGRMHILSKMNAVIQESRNRIKNHAPRIE
SIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVMIYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIMKFG
AFVSFLGGKKGLVHISEIRNEHISSVGSVISLNDKVKVLVIGIDREHIQLSMRRVDQESGEPIDGELYNIRKNSFSDDSC
GSTGGSSFKESYNPNSRHGSHEKKRSGGSSRSSRRNSNGPNYYREDLPSSNGFGNNNRSFSNSRNGHDVPRKPRFF

Sequences:

>Translated_796_residues
MFNLIRRSAEWGGKTLVLESGKIARQASGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTVQFVAKSYAVGKIPGGFLKRE
GKPSDRETLISRLIDRSIRPLFPAGFYDEISIVCNLLSYDTVTPPEVTALVGATAALSISGVPFNGLVVGARVGYLPSEG
KYLLNASADEMLCSSLDLFLSGNEDSVLMVESEASELSESQMLGAITFGHQHCQEVINLIKEFSHESGQTPIDFIPHDIS
SLVSDIESSYKEDFSLAYSNTIKKERVLKLEELRGKVLSEVADKYSAGDVECSDQDIVTALKTFERSLVRSKIIDTSSRI
DGRAFDEIRDIEIEVDVLPKAHGSALFTRGNTQALVVTALGTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAP
GRREIGHGKLAWRAIRYVLPEKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEDDRFI
ILSDILGDEDHLGDMDFKVAGTAEGVTALQMDMKISGIDIDIIEKALLQAKDGRMHILSKMNAVIQESRNRIKNHAPRIE
SIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVMIYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIMKFG
AFVSFLGGKKGLVHISEIRNEHISSVGSVISLNDKVKVLVIGIDREHIQLSMRRVDQESGEPIDGELYNIRKNSFSDDSC
GSTGGSSFKESYNPNSRHGSHEKKRSGGSSRSSRRNSNGPNYYREDLPSSNGFGNNNRSFSNSRNGHDVPRKPRFF
>Mature_796_residues
MFNLIRRSAEWGGKTLVLESGKIARQASGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTVQFVAKSYAVGKIPGGFLKRE
GKPSDRETLISRLIDRSIRPLFPAGFYDEISIVCNLLSYDTVTPPEVTALVGATAALSISGVPFNGLVVGARVGYLPSEG
KYLLNASADEMLCSSLDLFLSGNEDSVLMVESEASELSESQMLGAITFGHQHCQEVINLIKEFSHESGQTPIDFIPHDIS
SLVSDIESSYKEDFSLAYSNTIKKERVLKLEELRGKVLSEVADKYSAGDVECSDQDIVTALKTFERSLVRSKIIDTSSRI
DGRAFDEIRDIEIEVDVLPKAHGSALFTRGNTQALVVTALGTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAP
GRREIGHGKLAWRAIRYVLPEKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEDDRFI
ILSDILGDEDHLGDMDFKVAGTAEGVTALQMDMKISGIDIDIIEKALLQAKDGRMHILSKMNAVIQESRNRIKNHAPRIE
SIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVMIYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIMKFG
AFVSFLGGKKGLVHISEIRNEHISSVGSVISLNDKVKVLVIGIDREHIQLSMRRVDQESGEPIDGELYNIRKNSFSDDSC
GSTGGSSFKESYNPNSRHGSHEKKRSGGSSRSSRRNSNGPNYYREDLPSSNGFGNNNRSFSNSRNGHDVPRKPRFF

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=719, Percent_Identity=34.4923504867872, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4826690, Length=96, Percent_Identity=43.75, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI145693187, Length=702, Percent_Identity=49.8575498575499, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=703, Percent_Identity=32.2901849217639, Blast_Score=348, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=119, Percent_Identity=39.4957983193277, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362905, Length=700, Percent_Identity=34, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651641, Length=700, Percent_Identity=34, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651643, Length=700, Percent_Identity=34, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI161079377, Length=646, Percent_Identity=34.0557275541796, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI20129977, Length=90, Percent_Identity=40, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_EHRCR (Q2GGA4)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507529.1
- ProteinModelPortal:   Q2GGA4
- STRING:   Q2GGA4
- GeneID:   3927271
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0726
- TIGR:   ECH_0726
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q2GGA4
- ProtClustDB:   PRK11824
- BioCyc:   ECHA205920:ECH_0726-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 87104; Mature: 87104

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFNLIRRSAEWGGKTLVLESGKIARQASGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTV
CCHHHHHHHCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHH
QFVAKSYAVGKIPGGFLKREGKPSDRETLISRLIDRSIRPLFPAGFYDEISIVCNLLSYD
HHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
TVTPPEVTALVGATAALSISGVPFNGLVVGARVGYLPSEGKYLLNASADEMLCSSLDLFL
CCCCCHHHHHHCCHHEEEECCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHCCCEEE
SGNEDSVLMVESEASELSESQMLGAITFGHQHCQEVINLIKEFSHESGQTPIDFIPHDIS
ECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHH
SLVSDIESSYKEDFSLAYSNTIKKERVLKLEELRGKVLSEVADKYSAGDVECSDQDIVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LKTFERSLVRSKIIDTSSRIDGRAFDEIRDIEIEVDVLPKAHGSALFTRGNTQALVVTAL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCEEEECCCCCEEEEEEC
GTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAPGRREIGHGKLAWRAIRYVLP
CCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCC
EKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEDDRFI
CCCCCCEEEHHHHHHHHCCCCCHHHHHHCCHHEEEECCCCCCCCHHHHHHHHEECCCCEE
ILSDILGDEDHLGDMDFKVAGTAEGVTALQMDMKISGIDIDIIEKALLQAKDGRMHILSK
EEECCCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHH
MNAVIQESRNRIKNHAPRIESIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVM
HHHHHHHHHHHHHHCCCCHHEEEECHHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCEEE
IYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIMKFGAFVSFLGGKKGLVHISEIRN
EEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
EHISSVGSVISLNDKVKVLVIGIDREHIQLSMRRVDQESGEPIDGELYNIRKNSFSDDSC
HHHHHHCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
GSTGGSSFKESYNPNSRHGSHEKKRSGGSSRSSRRNSNGPNYYREDLPSSNGFGNNNRSF
CCCCCCHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
SNSRNGHDVPRKPRFF
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFNLIRRSAEWGGKTLVLESGKIARQASGAVMVSYAGTTVLATVVTGKTKEPVDFLPLTV
CCHHHHHHHCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHH
QFVAKSYAVGKIPGGFLKREGKPSDRETLISRLIDRSIRPLFPAGFYDEISIVCNLLSYD
HHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
TVTPPEVTALVGATAALSISGVPFNGLVVGARVGYLPSEGKYLLNASADEMLCSSLDLFL
CCCCCHHHHHHCCHHEEEECCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHCCCEEE
SGNEDSVLMVESEASELSESQMLGAITFGHQHCQEVINLIKEFSHESGQTPIDFIPHDIS
ECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHH
SLVSDIESSYKEDFSLAYSNTIKKERVLKLEELRGKVLSEVADKYSAGDVECSDQDIVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LKTFERSLVRSKIIDTSSRIDGRAFDEIRDIEIEVDVLPKAHGSALFTRGNTQALVVTAL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEECCCCCCCEEEECCCCCEEEEEEC
GTPQDEQIVDDLDGDRRENFLLHYNFPPYAVGESAALRAPGRREIGHGKLAWRAIRYVLP
CCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCC
EKSDFPYTIRVVSEITESDGSSSMATVCGASLALMDTGVPIKSPVAGIAMGLIKEDDRFI
CCCCCCEEEHHHHHHHHCCCCCHHHHHHCCHHEEEECCCCCCCCHHHHHHHHEECCCCEE
ILSDILGDEDHLGDMDFKVAGTAEGVTALQMDMKISGIDIDIIEKALLQAKDGRMHILSK
EEECCCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHH
MNAVIQESRNRIKNHAPRIESIFINKDKIRNVIGSGGKNIRDICEKTGAKIEIIQDGTVM
HHHHHHHHHHHHHHCCCCHHEEEECHHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCEEE
IYAVNNEAVEYAKSMIMDIVTEPEIGKVFEGTVVEIMKFGAFVSFLGGKKGLVHISEIRN
EEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
EHISSVGSVISLNDKVKVLVIGIDREHIQLSMRRVDQESGEPIDGELYNIRKNSFSDDSC
HHHHHHCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
GSTGGSSFKESYNPNSRHGSHEKKRSGGSSRSSRRNSNGPNYYREDLPSSNGFGNNNRSF
CCCCCCHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
SNSRNGHDVPRKPRFF
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA