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The map label for this gene is sucB [H]

Identifier: 88658260

GI number: 88658260

Start: 1092636

End: 1093850

Strand: Reverse

Name: sucB [H]

Synonym: ECH_1065

Alternate gene names: 88658260

Gene position: 1093850-1092636 (Counterclockwise)

Preceding gene: 88657661

Following gene: 88657622

Centisome position: 92.99

GC content: 34.4

Gene sequence:

>1215_bases
ATGAGTGAAATACAAGTAAAGGCTGAAAATCTTGGTGGTGAGTCAATATTAGAGGCTCCAATTAGAGTCTCAGTGAATGT
TGGTGATAGTGTGAAACAAGGTGATATGTTATTTATCATAGAAACTGACAAGACCTCGCTTGAAATTGTATCTCCTGAAG
ATGGTATTATTAATGAAATATTTGTTGTTGATGAGGAAATCATACAGAGAGGGCAAGTTTTATGTACAATAAATACTGTA
AAATCGAATGCTGTTAAGCCATCTGAGGGGAACACTGCACATAGTACTACTGTTACAGTAGCTGATGACATGCAACAATT
TATACAGAAAAAGGATGCTCCATCTGCAATGAAGATTATGGAAGAAAATGTGATTGATAAAAGTCAGGTGAGTGGTTCTG
GTATTGGAGGTCGCATTACAAAATCTGATGTGTTAAATTATATGAAACTTGCTTCTGAAGAAGATAATACTAAAGCAAAT
AGTATTAGTAGTTTATCTGTTGTATCTGAAGAGAAAAGAGAAGAACGTGTAAAAATGAGTAAGATCAGACAGGTAATTGC
TGCAAGATTAAAGGAATCTCAAAATACTGCTGCGATATTAACAACTTTTAACGAAGTTGATATGAAGAATGTGATGGATC
TTCGTGCTAAGTATAGAGAAACTTTTGAAAAGAAATATGGTATTAAGCTTGGTTTTATGTCGTTCTTTATAAAGGCAGTG
GTGCTAGCATTAAAAGAACTACCAATAATCAATGCTGAGATATCAGGTAATGAGATTGTTTATAAGCATTATTATGACAT
GGGTATTGCAGTAGGAACAGATAAGGGATTGGTTGTTCCAGTAATACGTGATGCTGATAAAATGTCTTTTGCTGATCTTG
AATCAACGTTAGCTTCTTTAGGGAAAAAAGCTAGGGAAGGAAAGTTGGAAGTTGCTGATATGGCAGGTGCAACATTTACT
ATCACCAATGGAGGTGTGTATGGGTCTTTGCTGTCTACTCCTATAATTAATCCTCCTCAGTCTGGAATTTTAGGGATGCA
TTCAATACAAAAGCGTCCTGTTGCAATTGATGATAAGACTATAGAAATTAGACCAATGATGTATATTGCTTTATCTTATG
ATCATAGAATTGTTGATGGACAAGGTGCTGTTACATTTTTAGTTAGAATAAAGCAATATATTGAAGATCCTAGTAGGATG
TTTTTAGAGGTATAA

Upstream 100 bases:

>100_bases
GAATTTTTGTCATGGTTTGAATCATAATTGTGAGTCATGCTTATGGTATAGTGGCATGTACAACTAAGTCTGTGTAGAGA
GTTTTTTTAAGGTACATTTT

Downstream 100 bases:

>100_bases
TTCTGGTTGTTGTTAATTATGGAATGTACTTAAATGCTTTCTTGAAGATAGTCTTGTAAAATATAGAGCTCATGGAAAGT
TTATGTTCTATTATGTCTAA

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSEIQVKAENLGGESILEAPIRVSVNVGDSVKQGDMLFIIETDKTSLEIVSPEDGIINEIFVVDEEIIQRGQVLCTINTV
KSNAVKPSEGNTAHSTTVTVADDMQQFIQKKDAPSAMKIMEENVIDKSQVSGSGIGGRITKSDVLNYMKLASEEDNTKAN
SISSLSVVSEEKREERVKMSKIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAKYRETFEKKYGIKLGFMSFFIKAV
VLALKELPIINAEISGNEIVYKHYYDMGIAVGTDKGLVVPVIRDADKMSFADLESTLASLGKKAREGKLEVADMAGATFT
ITNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAIDDKTIEIRPMMYIALSYDHRIVDGQGAVTFLVRIKQYIEDPSRM
FLEV

Sequences:

>Translated_404_residues
MSEIQVKAENLGGESILEAPIRVSVNVGDSVKQGDMLFIIETDKTSLEIVSPEDGIINEIFVVDEEIIQRGQVLCTINTV
KSNAVKPSEGNTAHSTTVTVADDMQQFIQKKDAPSAMKIMEENVIDKSQVSGSGIGGRITKSDVLNYMKLASEEDNTKAN
SISSLSVVSEEKREERVKMSKIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAKYRETFEKKYGIKLGFMSFFIKAV
VLALKELPIINAEISGNEIVYKHYYDMGIAVGTDKGLVVPVIRDADKMSFADLESTLASLGKKAREGKLEVADMAGATFT
ITNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAIDDKTIEIRPMMYIALSYDHRIVDGQGAVTFLVRIKQYIEDPSRM
FLEV
>Mature_403_residues
SEIQVKAENLGGESILEAPIRVSVNVGDSVKQGDMLFIIETDKTSLEIVSPEDGIINEIFVVDEEIIQRGQVLCTINTVK
SNAVKPSEGNTAHSTTVTVADDMQQFIQKKDAPSAMKIMEENVIDKSQVSGSGIGGRITKSDVLNYMKLASEEDNTKANS
ISSLSVVSEEKREERVKMSKIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAKYRETFEKKYGIKLGFMSFFIKAVV
LALKELPIINAEISGNEIVYKHYYDMGIAVGTDKGLVVPVIRDADKMSFADLESTLASLGKKAREGKLEVADMAGATFTI
TNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAIDDKTIEIRPMMYIALSYDHRIVDGQGAVTFLVRIKQYIEDPSRMF
LEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=57.4468085106383, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=29.6728971962617, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=414, Percent_Identity=29.2270531400966, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI203098753, Length=428, Percent_Identity=27.1028037383178, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI203098816, Length=428, Percent_Identity=27.1028037383178, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=32.9192546583851, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=379, Percent_Identity=44.5910290237467, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=27.1662763466042, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=41.1910669975186, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17560088, Length=415, Percent_Identity=30.3614457831325, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=27.1662763466042, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=235, Percent_Identity=30.6382978723404, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=391, Percent_Identity=40.4092071611253, Blast_Score=301, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6324258, Length=428, Percent_Identity=25.7009345794392, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=58.1497797356828, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=27.511961722488, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44471; Mature: 44340

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQVKAENLGGESILEAPIRVSVNVGDSVKQGDMLFIIETDKTSLEIVSPEDGIINEI
CCCEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCEE
FVVDEEIIQRGQVLCTINTVKSNAVKPSEGNTAHSTTVTVADDMQQFIQKKDAPSAMKIM
EEECHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHH
EENVIDKSQVSGSGIGGRITKSDVLNYMKLASEEDNTKANSISSLSVVSEEKREERVKMS
HHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAKYRETFEKKYGIKLGFMSFFIKAV
HHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
VLALKELPIINAEISGNEIVYKHYYDMGIAVGTDKGLVVPVIRDADKMSFADLESTLASL
HHHHHHCCEEEEEECCCEEEEEEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
GKKAREGKLEVADMAGATFTITNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAIDDKT
HHHHHCCCEEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHCCCCEEECCCE
IEIRPMMYIALSYDHRIVDGQGAVTFLVRIKQYIEDPSRMFLEV
EEEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
SEIQVKAENLGGESILEAPIRVSVNVGDSVKQGDMLFIIETDKTSLEIVSPEDGIINEI
CCEEEECCCCCCHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCEE
FVVDEEIIQRGQVLCTINTVKSNAVKPSEGNTAHSTTVTVADDMQQFIQKKDAPSAMKIM
EEECHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHH
EENVIDKSQVSGSGIGGRITKSDVLNYMKLASEEDNTKANSISSLSVVSEEKREERVKMS
HHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KIRQVIAARLKESQNTAAILTTFNEVDMKNVMDLRAKYRETFEKKYGIKLGFMSFFIKAV
HHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
VLALKELPIINAEISGNEIVYKHYYDMGIAVGTDKGLVVPVIRDADKMSFADLESTLASL
HHHHHHCCEEEEEECCCEEEEEEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
GKKAREGKLEVADMAGATFTITNGGVYGSLLSTPIINPPQSGILGMHSIQKRPVAIDDKT
HHHHHCCCEEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCCCCCHHHHCCCCEEECCCE
IEIRPMMYIALSYDHRIVDGQGAVTFLVRIKQYIEDPSRMFLEV
EEEEEEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA