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The map label for this gene is groEL [H]

Identifier: 88658066

GI number: 88658066

Start: 353838

End: 355490

Strand: Direct

Name: groEL [H]

Synonym: ECH_0365

Alternate gene names: 88658066

Gene position: 353838-355490 (Clockwise)

Preceding gene: 88658095

Following gene: 88658213

Centisome position: 30.08

GC content: 34.6

Gene sequence:

>1653_bases
ATGGCAAATGTAGTTGTGACTGGTGAACAACTTGATAAATCAATTAGAGAAGTTGTTCGTATTTTAGAAGATGCTGTAGG
ATGTACGGCTGGGCCTAAGGGACTTACTGTAGCTATTGGTAAATCTTATGGAGCTCCAGAAGTTACAAAAGATGGATATA
AAGTTATTAAGAGTATAAAGCCAGAGGATCCATTGGCTCTTGCTATTGCTAATATAATTACTCAGAGTGCTTCTCAATGT
AATGATAAAGTTGGTGATGGAACAACTACATGTTCTATTTTAACTGCAAAAGTTATAGAAGAAGTATCTAAAGCTAAAGC
TGCTGGAGCAGATATTGTGTGTATTAAAGAAGGTGTACTAAAAGCTAAAGAGGCTGTTTTGGAAGCATTAATGTCTATGA
AGCGTGAAGTGTTGTCTGAAGAAGAAATTGCACAAGTTGCTACTATTTCAGCTAATGGAGATAAGAATATAGGTAGTAAG
ATTGCACAATGTGTTCAAGAAGTTGGTAAAGATGGGGTTATTACAGTTGAAGAAAGTAAAGGCTTTAAAGAATTAGATGT
TGAAAAAACTGATGGTATGCAGTTTGATCGTGGATACCTTTCTCCTTATTTTGTAACTAATTCAGAAAAGATGTTAGTGG
AATTTGAAAATCCTTATATCTTACTAACAGAGAAAAAACTTAATATCATACAACCTATATTACCAATTTTGGAAAATGTG
GCTAGATCAGGAAGACCTCTTTTAATTATTGCAGAAGATGTGGAAGGTGAAGCACTTAGCACTCTCGTTTTAAATAAATT
GCGCGGTGGGTTACATGTAGCAGCAGTTAAAGCGCCAGGGTTTGGTGATAGAAGAAAAGATATGTTAGGTGATATTGCTA
TTTTAACTGGAGCTAAACATGTAATAAGTGATGATCTTGCAATAAAAATGGAAGATCTAACTTTAGCTGAATTAGGTACT
GCTAAAAATATACGTATTACAAAAGATACTACTACTATTATCGGTAGTGTAGATAATAGTTCTGCTAATGTACAAAGTAG
AATTAATCAAATTAAAATGCAAATTGAAGCTTCTACATCAGATTATGACAAAGAAAAATTAAGAGAGCGTTTAGCTAAGC
TATCAGGTGGTGTTGCTGTATTGAAAGTTGGTGGGTCTAGTGAAGTTGAAGTTAAAGAACGTAAAGACCGTGTTGAAGAT
GCTTTACACGCAACTAGAGCTGCTGTTGAAGAAGGTGTTGTACCAGGAGGTGGTGCTGCATTATTATATACATTATCAGT
TCTTGAAAACTTGAAGAGTAAAAATGATGATGAACAATTAGGTATTAATATTGTAAAACGCGCTTTACAAGCTCCTATTA
AACGTATCATTAAAAACTCTGGATCAGAAAATGCTCCATGTGTTATTGCTCATTTGTTAAAACAAAATGATAAAGAGCTT
ATTTTTAATGTTGATACAATGAATTTTGCAAATGCTTTTACTTCTGGGGTTATTGATCCACTGAAGGTAGTGCGTATAGC
ATTTGATTTTGCTGTATCTCTTGCAGCAGTATTTATGACTCTAAATGCTATTGTAGTAGATGTACCATCTAAAGATGATG
CTAATGCTGGTGCTGGAGGCATGGGAGGCATGGGAGGTATGGGAGGATTCTAA

Upstream 100 bases:

>100_bases
TCATTACATTGAGTTTAAATATGCTATACATGTGTTTTTTTGTAATAGCCTAAGTATAAGTAGTTTTAATTAATTTTTTA
TTAAACGGAGATTAAATAAC

Downstream 100 bases:

>100_bases
TTAATAGATAATCTTCTATATTGACTAATGGGGTAAAGTCTTTTAAGACTTTGCCCCCTTCTTTTAGTTTATGTTTTTCA
AATATATTGCCTATGTAATA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MANVVVTGEQLDKSIREVVRILEDAVGCTAGPKGLTVAIGKSYGAPEVTKDGYKVIKSIKPEDPLALAIANIITQSASQC
NDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKEGVLKAKEAVLEALMSMKREVLSEEEIAQVATISANGDKNIGSK
IAQCVQEVGKDGVITVEESKGFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENV
ARSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKHVISDDLAIKMEDLTLAELGT
AKNIRITKDTTTIIGSVDNSSANVQSRINQIKMQIEASTSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVED
ALHATRAAVEEGVVPGGGAALLYTLSVLENLKSKNDDEQLGINIVKRALQAPIKRIIKNSGSENAPCVIAHLLKQNDKEL
IFNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDANAGAGGMGGMGGMGGF

Sequences:

>Translated_550_residues
MANVVVTGEQLDKSIREVVRILEDAVGCTAGPKGLTVAIGKSYGAPEVTKDGYKVIKSIKPEDPLALAIANIITQSASQC
NDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKEGVLKAKEAVLEALMSMKREVLSEEEIAQVATISANGDKNIGSK
IAQCVQEVGKDGVITVEESKGFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENV
ARSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKHVISDDLAIKMEDLTLAELGT
AKNIRITKDTTTIIGSVDNSSANVQSRINQIKMQIEASTSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVED
ALHATRAAVEEGVVPGGGAALLYTLSVLENLKSKNDDEQLGINIVKRALQAPIKRIIKNSGSENAPCVIAHLLKQNDKEL
IFNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDANAGAGGMGGMGGMGGF
>Mature_549_residues
ANVVVTGEQLDKSIREVVRILEDAVGCTAGPKGLTVAIGKSYGAPEVTKDGYKVIKSIKPEDPLALAIANIITQSASQCN
DKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKEGVLKAKEAVLEALMSMKREVLSEEEIAQVATISANGDKNIGSKI
AQCVQEVGKDGVITVEESKGFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENVA
RSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKHVISDDLAIKMEDLTLAELGTA
KNIRITKDTTTIIGSVDNSSANVQSRINQIKMQIEASTSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDA
LHATRAAVEEGVVPGGGAALLYTLSVLENLKSKNDDEQLGINIVKRALQAPIKRIIKNSGSENAPCVIAHLLKQNDKELI
FNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDANAGAGGMGGMGGMGGF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=514, Percent_Identity=46.4980544747082, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI31542947, Length=514, Percent_Identity=46.4980544747082, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1790586, Length=518, Percent_Identity=51.3513513513513, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17555558, Length=517, Percent_Identity=48.1624758220503, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI193210679, Length=225, Percent_Identity=49.7777777777778, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323288, Length=512, Percent_Identity=46.2890625, Blast_Score=433, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24641193, Length=516, Percent_Identity=47.4806201550388, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24641191, Length=516, Percent_Identity=47.4806201550388, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45550936, Length=513, Percent_Identity=44.4444444444444, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45550132, Length=513, Percent_Identity=44.4444444444444, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45550935, Length=513, Percent_Identity=44.4444444444444, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17864606, Length=531, Percent_Identity=40.8662900188324, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=33.3333333333333, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=33.3333333333333, Blast_Score=263, Evalue=3e-70,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58545; Mature: 58414

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVTGEQLDKSIREVVRILEDAVGCTAGPKGLTVAIGKSYGAPEVTKDGYKVIKSIK
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
PEDPLALAIANIITQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKEGVL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
KAKEAVLEALMSMKREVLSEEEIAQVATISANGDKNIGSKIAQCVQEVGKDGVITVEESK
HHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCC
GFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENV
CCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHH
ARSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKH
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHH
VISDDLAIKMEDLTLAELGTAKNIRITKDTTTIIGSVDNSSANVQSRINQIKMQIEASTS
HHCCCCEEEECCCCHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHEEEEECCCC
DYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDALHATRAAVEEGVVPGGGAA
CCCHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LLYTLSVLENLKSKNDDEQLGINIVKRALQAPIKRIIKNSGSENAPCVIAHLLKQNDKEL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
IFNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDANAGAGG
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
MGGMGGMGGF
CCCCCCCCCC
>Mature Secondary Structure 
ANVVVTGEQLDKSIREVVRILEDAVGCTAGPKGLTVAIGKSYGAPEVTKDGYKVIKSIK
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
PEDPLALAIANIITQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKEGVL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
KAKEAVLEALMSMKREVLSEEEIAQVATISANGDKNIGSKIAQCVQEVGKDGVITVEESK
HHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCC
GFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEFENPYILLTEKKLNIIQPILPILENV
CCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHH
ARSGRPLLIIAEDVEGEALSTLVLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKH
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHH
VISDDLAIKMEDLTLAELGTAKNIRITKDTTTIIGSVDNSSANVQSRINQIKMQIEASTS
HHCCCCEEEECCCCHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHEEEEECCCC
DYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDALHATRAAVEEGVVPGGGAA
CCCHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LLYTLSVLENLKSKNDDEQLGINIVKRALQAPIKRIIKNSGSENAPCVIAHLLKQNDKEL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEE
IFNVDTMNFANAFTSGVIDPLKVVRIAFDFAVSLAAVFMTLNAIVVDVPSKDDANAGAGG
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
MGGMGGMGGF
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA