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The map label for this gene is lepA [H]

Identifier: 88658022

GI number: 88658022

Start: 732921

End: 734717

Strand: Reverse

Name: lepA [H]

Synonym: ECH_0724

Alternate gene names: 88658022

Gene position: 734717-732921 (Counterclockwise)

Preceding gene: 88657758

Following gene: 88657581

Centisome position: 62.46

GC content: 33.06

Gene sequence:

>1797_bases
ATGGATGAAAGTCATATAAGAAATTTTGCTATAATAGCTCATATTGATCATGGTAAGTCTACGTTAGCTGATCGGTTAAT
AGAAGAATGTAATGGTTTGGATGAACGGGAGATGAAGGATCAAGTACTTGATTCCATGGATATTGAAAGAGAACGTGGTA
TTACAATTAAAGCACAAACTGTTAGATTAGCATATAAAGCTAAAGATGGACAAACTTATTACTTAAATTTAATGGATACA
CCAGGACATGTGGATTTTTCATATGAGGTAAGTAGAAGTTTGGCTGCTTGTGAGGGATCATTACTTATTGTAGATAGTAG
TCAGGGAGTTGAAGCACAAACTTTAGCAAATGTATATAAGGCTATAGATAGTAATCATGAGATTATCCCAGTGCTAAATA
AGATAGATTTAGCATCTTCAGATCCAGACAAAGTAAAGTTGCAAATAGAAGATATGATTGGTATTGACGCAAGTGAGTCA
CTACTTGTGTCAGCAAAGTCAGGTATTGGTATACAAGATGTATTAGAAGCTATTGTTTCTAGATTACCAGCTCCATCTGG
GAAATCAGAGAATCCGTTAAAAGCTATACTAGTTGATACTTGGTATGATACATATCTTGGTATAGTAATTTTGTTGCGTA
TTAAAGATGGTGTTATAAGAAAGGGCATGAAAATTGTCATGATGTCAAATAATGCTGTGTATCAAGTTGATAATGTAGGT
ATTTTTACCCCGCATAAGAAAGTTGTGGATCAACTTTCAGTAGGTGAAATTGGATTTATTACTGCATCTATTAAAGAATT
ATCTGATTGTAAAGTGGGAGATACAATTACTGAAGAGCAGAGAAGATGTAGTGAACCACTTCCTGGATTTAGGACGATTC
ATCCTGTAGTGTTTTGTAGTATTTTTCCAAATGAAGCTGGAGAATTTGAAAGATTACGTGAAGCTTTGAAAAAGTTACAA
CTCAATGATGCAAGTTTTACTTTTGAAATTGAGGTTTCTAATGCGCTTGGATATGGATTCCGTTGTGGGTTTTTAGGAAT
GTTACATTTAGAAGTAATTCAAGAAAGGTTAGAAAGGGAATTTAATTTAGATTTGACTGCTACAGCTCCAGGGGTAATCT
ATCAAGTTACTACTAGAAGTGGTGATGTTCGTAAAGTTCATAATCCTCATGATTTTGGTGAATCGCAAGATATTGCTAAT
ATTAAGGAGCCATGGATCTGTGCTACTATTATGGTACCTGATCAATATTTAGGTGTTATTATGTCATTATGTAATAATAA
AAGAGGTGAAAAACTAGATTTGTCATATTCAGGTAATACAGCATTGTTAAAATATAGATTACCATTATCTGAAGTTGTGT
TTGATTTTTATGATAGAATAAAGTCTATGTCTAAGGGTTATGCTAGTTTAGATTGGGAAATGGATGAATATTTGGATAGT
GAAATTGCTAAATTAAGTATTCTAATAAATTCTGAACCAGTAGATGCTCTTGCATGTATTATTCATAAAAGTAAAGTGGA
ACAGAGAGGACGTGAAATATGTCTTAGGTTGAAAGATTTAATCCCAAGACAACAATATAAAATTGCAATACAAGCAGCAG
TGGGAGGAAGAATTGTTGCTCGTGAAACTATTTCTCCGTATCGTAAAGATGTTACAGCAAAATTATATGGTGGAGATGTT
ACCAGAAGGATGAAATTATTAGAAAAACAGAAGAAAGGAAAGAAAAGATTGCGATCTATAGGGAATGTTAATGTTCCGCA
TAATGCGTTTATTCAAGCGTTAAAAATAATAGATTAG

Upstream 100 bases:

>100_bases
ATTTTTCAGTAGATAAAATTAGTATTCCTGCTACTTTTGAGATTATTACTTTGAAGGGAAGTAAAGTGTAAAGGTTATAA
AGGTGTGAGGATAAATTGAA

Downstream 100 bases:

>100_bases
CTTTTTTATGTTGTTATAAAAATGTAATATTTTTGCTAAATACAATTATTAATTTTTTAATTTTAATTAATTAGTAGTAT
AGGTATTATAATTAAATATT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MDESHIRNFAIIAHIDHGKSTLADRLIEECNGLDEREMKDQVLDSMDIERERGITIKAQTVRLAYKAKDGQTYYLNLMDT
PGHVDFSYEVSRSLAACEGSLLIVDSSQGVEAQTLANVYKAIDSNHEIIPVLNKIDLASSDPDKVKLQIEDMIGIDASES
LLVSAKSGIGIQDVLEAIVSRLPAPSGKSENPLKAILVDTWYDTYLGIVILLRIKDGVIRKGMKIVMMSNNAVYQVDNVG
IFTPHKKVVDQLSVGEIGFITASIKELSDCKVGDTITEEQRRCSEPLPGFRTIHPVVFCSIFPNEAGEFERLREALKKLQ
LNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLEREFNLDLTATAPGVIYQVTTRSGDVRKVHNPHDFGESQDIAN
IKEPWICATIMVPDQYLGVIMSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSMSKGYASLDWEMDEYLDS
EIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAVGGRIVARETISPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLRSIGNVNVPHNAFIQALKIID

Sequences:

>Translated_598_residues
MDESHIRNFAIIAHIDHGKSTLADRLIEECNGLDEREMKDQVLDSMDIERERGITIKAQTVRLAYKAKDGQTYYLNLMDT
PGHVDFSYEVSRSLAACEGSLLIVDSSQGVEAQTLANVYKAIDSNHEIIPVLNKIDLASSDPDKVKLQIEDMIGIDASES
LLVSAKSGIGIQDVLEAIVSRLPAPSGKSENPLKAILVDTWYDTYLGIVILLRIKDGVIRKGMKIVMMSNNAVYQVDNVG
IFTPHKKVVDQLSVGEIGFITASIKELSDCKVGDTITEEQRRCSEPLPGFRTIHPVVFCSIFPNEAGEFERLREALKKLQ
LNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLEREFNLDLTATAPGVIYQVTTRSGDVRKVHNPHDFGESQDIAN
IKEPWICATIMVPDQYLGVIMSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSMSKGYASLDWEMDEYLDS
EIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAVGGRIVARETISPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLRSIGNVNVPHNAFIQALKIID
>Mature_598_residues
MDESHIRNFAIIAHIDHGKSTLADRLIEECNGLDEREMKDQVLDSMDIERERGITIKAQTVRLAYKAKDGQTYYLNLMDT
PGHVDFSYEVSRSLAACEGSLLIVDSSQGVEAQTLANVYKAIDSNHEIIPVLNKIDLASSDPDKVKLQIEDMIGIDASES
LLVSAKSGIGIQDVLEAIVSRLPAPSGKSENPLKAILVDTWYDTYLGIVILLRIKDGVIRKGMKIVMMSNNAVYQVDNVG
IFTPHKKVVDQLSVGEIGFITASIKELSDCKVGDTITEEQRRCSEPLPGFRTIHPVVFCSIFPNEAGEFERLREALKKLQ
LNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLEREFNLDLTATAPGVIYQVTTRSGDVRKVHNPHDFGESQDIAN
IKEPWICATIMVPDQYLGVIMSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSMSKGYASLDWEMDEYLDS
EIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAVGGRIVARETISPYRKDVTAKLYGGDV
TRRMKLLEKQKKGKKRLRSIGNVNVPHNAFIQALKIID

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=600, Percent_Identity=46.1666666666667, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=43.0656934306569, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=37.8378378378378, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=34.5911949685535, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI25306283, Length=136, Percent_Identity=43.3823529411765, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI19923640, Length=136, Percent_Identity=43.3823529411765, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI25306287, Length=136, Percent_Identity=43.3823529411765, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=40.3508771929825, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=40.3508771929825, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=37.8571428571429, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=40.3508771929825, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=37.8571428571429, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI53729339, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI53729337, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI34147630, Length=269, Percent_Identity=24.907063197026, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=53.8851351351351, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=504, Percent_Identity=30.1587301587302, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789738, Length=156, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790835, Length=158, Percent_Identity=31.6455696202532, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789559, Length=220, Percent_Identity=25.9090909090909, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=38.8524590163934, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17556745, Length=200, Percent_Identity=33.5, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.8059701492537, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.8059701492537, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=36.551724137931, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=139, Percent_Identity=35.9712230215827, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=219, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=338, Percent_Identity=23.9644970414201, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=44.1471571906354, Blast_Score=528, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=159, Percent_Identity=36.4779874213836, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=151, Percent_Identity=36.4238410596026, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=151, Percent_Identity=36.4238410596026, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=136, Percent_Identity=37.5, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=36.3013698630137, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=43.3774834437086, Blast_Score=520, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=34.5911949685535, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=41.304347826087, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=35.5555555555556, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=34.6666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=34.6666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=34.6666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221458488, Length=153, Percent_Identity=35.9477124183007, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363316, Length=284, Percent_Identity=23.943661971831, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864358, Length=284, Percent_Identity=23.943661971831, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921738, Length=280, Percent_Identity=27.1428571428571, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66987; Mature: 66987

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDESHIRNFAIIAHIDHGKSTLADRLIEECNGLDEREMKDQVLDSMDIERERGITIKAQT
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLAYKAKDGQTYYLNLMDTPGHVDFSYEVSRSLAACEGSLLIVDSSQGVEAQTLANVYK
EEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AIDSNHEIIPVLNKIDLASSDPDKVKLQIEDMIGIDASESLLVSAKSGIGIQDVLEAIVS
HHCCCCCEEEEECEECCCCCCCCEEEEEEEHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
RLPAPSGKSENPLKAILVDTWYDTYLGIVILLRIKDGVIRKGMKIVMMSNNAVYQVDNVG
HCCCCCCCCCCCEEEEEEEHHHHHHHHHHEEEEECCCHHHCCCEEEEEECCEEEEECCCE
IFTPHKKVVDQLSVGEIGFITASIKELSDCKVGDTITEEQRRCSEPLPGFRTIHPVVFCS
EECCCHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHCCCEEEEE
IFPNEAGEFERLREALKKLQLNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLERE
ECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FNLDLTATAPGVIYQVTTRSGDVRKVHNPHDFGESQDIANIKEPWICATIMVPDQYLGVI
CCCEEEECCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
MSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSMSKGYASLDWEMDEYLDS
HHHHCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHH
EIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAVGGRIVA
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEE
RETISPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLRSIGNVNVPHNAFIQALKIID
EHHCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDESHIRNFAIIAHIDHGKSTLADRLIEECNGLDEREMKDQVLDSMDIERERGITIKAQT
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLAYKAKDGQTYYLNLMDTPGHVDFSYEVSRSLAACEGSLLIVDSSQGVEAQTLANVYK
EEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AIDSNHEIIPVLNKIDLASSDPDKVKLQIEDMIGIDASESLLVSAKSGIGIQDVLEAIVS
HHCCCCCEEEEECEECCCCCCCCEEEEEEEHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
RLPAPSGKSENPLKAILVDTWYDTYLGIVILLRIKDGVIRKGMKIVMMSNNAVYQVDNVG
HCCCCCCCCCCCEEEEEEEHHHHHHHHHHEEEEECCCHHHCCCEEEEEECCEEEEECCCE
IFTPHKKVVDQLSVGEIGFITASIKELSDCKVGDTITEEQRRCSEPLPGFRTIHPVVFCS
EECCCHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHCCCEEEEE
IFPNEAGEFERLREALKKLQLNDASFTFEIEVSNALGYGFRCGFLGMLHLEVIQERLERE
ECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
FNLDLTATAPGVIYQVTTRSGDVRKVHNPHDFGESQDIANIKEPWICATIMVPDQYLGVI
CCCEEEECCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
MSLCNNKRGEKLDLSYSGNTALLKYRLPLSEVVFDFYDRIKSMSKGYASLDWEMDEYLDS
HHHHCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHH
EIAKLSILINSEPVDALACIIHKSKVEQRGREICLRLKDLIPRQQYKIAIQAAVGGRIVA
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEE
RETISPYRKDVTAKLYGGDVTRRMKLLEKQKKGKKRLRSIGNVNVPHNAFIQALKIID
EHHCCHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA