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The map label for this gene is trmD [H]

Identifier: 88658005

GI number: 88658005

Start: 1516

End: 2223

Strand: Direct

Name: trmD [H]

Synonym: ECH_0003

Alternate gene names: 88658005

Gene position: 1516-2223 (Clockwise)

Preceding gene: 88658505

Following gene: 88658630

Centisome position: 0.13

GC content: 34.32

Gene sequence:

>708_bases
GTGACATTCAACGTTAACGTTTTAACCATATTTCCAGAAATGTTTCCAGGAACATTAAGCCATTCAATAATAGGTAGAGC
CCTAAATAAAGGCCTTTGGAACCTTAATGTTATCGATATAAGATCATTTGCAAAAGATAAACATAAAACTGTAGATGACA
GGCCTTACGGCGGTGGACCTGGAATGATAATGAAAGCAGATGTTATAGGCTCAGCAATAGATGAAGTATTAAGTAAAAAC
AAAAACACAAAACTAATATACATGTCCCCCAGTGGAATAAAATTAAATCAAGATATTTCTGGACAATTAACACATTTTTC
TAATATAACTATACTATGTGGTCGCTTTGAAGGAATAGATAAGAGAATTTTAGATTTTTATGATTTTCATGAAATAAGTA
TTGGAGATTATATATTATCTGGTGGAGAAGTTGCTTGTATGGTTCTCATAGAGACTTGTGTCAGGCTTATACCTGGGGTA
GTTAGTAATACACAAAGTATACGTGATGAAAGTTTTACCTTAGACTATAAGTTAGAATACCCTCAGTACACACGTCCAGC
AAAATGGAGGGGATTAGAGGTCCCTGATGTTTTACTCTCTGGAAATCATAAAAAGATTAACCTTTGGAAAGCCAATCAAT
CTTACTGTATAACCAAGCAAAGGCGTCCTGAATTGACTGGTACCATAGATGGAGATATATATGAGTGA

Upstream 100 bases:

>100_bases
AAAAGCACTATGTTACCTTTTACAAAAGATATATTCCCCTATGTCAATGTCAAAGAAAAATACATAATAATAACATTGCC
AGAAGTTATAGGAAATAACA

Downstream 100 bases:

>100_bases
TTTGCTTAAGGAGTTCAATGAACAACAAATGAAATTGTTGTCCAACAAAGAAATACCAAAGTTTAGTTCAGGTGATACAC
TAAGAGTAAGCATGAAAATT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTFNVNVLTIFPEMFPGTLSHSIIGRALNKGLWNLNVIDIRSFAKDKHKTVDDRPYGGGPGMIMKADVIGSAIDEVLSKN
KNTKLIYMSPSGIKLNQDISGQLTHFSNITILCGRFEGIDKRILDFYDFHEISIGDYILSGGEVACMVLIETCVRLIPGV
VSNTQSIRDESFTLDYKLEYPQYTRPAKWRGLEVPDVLLSGNHKKINLWKANQSYCITKQRRPELTGTIDGDIYE

Sequences:

>Translated_235_residues
MTFNVNVLTIFPEMFPGTLSHSIIGRALNKGLWNLNVIDIRSFAKDKHKTVDDRPYGGGPGMIMKADVIGSAIDEVLSKN
KNTKLIYMSPSGIKLNQDISGQLTHFSNITILCGRFEGIDKRILDFYDFHEISIGDYILSGGEVACMVLIETCVRLIPGV
VSNTQSIRDESFTLDYKLEYPQYTRPAKWRGLEVPDVLLSGNHKKINLWKANQSYCITKQRRPELTGTIDGDIYE
>Mature_234_residues
TFNVNVLTIFPEMFPGTLSHSIIGRALNKGLWNLNVIDIRSFAKDKHKTVDDRPYGGGPGMIMKADVIGSAIDEVLSKNK
NTKLIYMSPSGIKLNQDISGQLTHFSNITILCGRFEGIDKRILDFYDFHEISIGDYILSGGEVACMVLIETCVRLIPGVV
SNTQSIRDESFTLDYKLEYPQYTRPAKWRGLEVPDVLLSGNHKKINLWKANQSYCITKQRRPELTGTIDGDIYE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=42.7927927927928, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26451; Mature: 26320

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNVNVLTIFPEMFPGTLSHSIIGRALNKGLWNLNVIDIRSFAKDKHKTVDDRPYGGGP
CEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC
GMIMKADVIGSAIDEVLSKNKNTKLIYMSPSGIKLNQDISGQLTHFSNITILCGRFEGID
CCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCEEEECCEEEEEECCCCCH
KRILDFYDFHEISIGDYILSGGEVACMVLIETCVRLIPGVVSNTQSIRDESFTLDYKLEY
HHHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHHCHHHCCCHHCCCCCEEEEEEECC
PQYTRPAKWRGLEVPDVLLSGNHKKINLWKANQSYCITKQRRPELTGTIDGDIYE
CCCCCCCCCCCCCCCHHEECCCCCEEEEEECCCCEEEECCCCCCCCEECCCCCCC
>Mature Secondary Structure 
TFNVNVLTIFPEMFPGTLSHSIIGRALNKGLWNLNVIDIRSFAKDKHKTVDDRPYGGGP
EEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC
GMIMKADVIGSAIDEVLSKNKNTKLIYMSPSGIKLNQDISGQLTHFSNITILCGRFEGID
CCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCEEEECCEEEEEECCCCCH
KRILDFYDFHEISIGDYILSGGEVACMVLIETCVRLIPGVVSNTQSIRDESFTLDYKLEY
HHHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHHCHHHCCCHHCCCCCEEEEEEECC
PQYTRPAKWRGLEVPDVLLSGNHKKINLWKANQSYCITKQRRPELTGTIDGDIYE
CCCCCCCCCCCCCCCHHEECCCCCEEEEEECCCCEEEECCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA