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The map label for this gene is gltA [H]

Identifier: 88657897

GI number: 88657897

Start: 111706

End: 112956

Strand: Direct

Name: gltA [H]

Synonym: ECH_0124

Alternate gene names: 88657897

Gene position: 111706-112956 (Clockwise)

Preceding gene: 88658530

Following gene: 88658400

Centisome position: 9.5

GC content: 31.33

Gene sequence:

>1251_bases
ATGGTTAAAGAAGCAGTATTAGAATTGGATGGTCAAAGAATAAAATTGCCGGTGTTATATGATACTCATGGGAATAAGGC
TGTAGATATTACTTCTTTATATAAAAATACAGGAATATTAACTTATGATCCAGGATTTATGTCTACTGCTGCTTGTGAAT
CTAGGATTACATTTATTGATGGGAATAAAGGTATATTATGTTATAGGGGACATGATATAGTAAATTTAGTAAATAATAAT
AAAAGTTTTATAGAAATAGTGTATTTGTTATTTTATGGTGTTCTCCCTGGTCTTGAGGATTTGCAAAATTTTATATCGCA
AATTTCTCAGGAATATAACCCTCCTAAGATAGTTCATGATGTTATAAAATCATTCCCACGAGATTCACATCCTATGTCTA
TATTAATGGCATGTTTTTCTGCGTTAGCATCATGTTATCATAATCAGGATAATAGTGCAGATCAATTAAAGTGTGCTAAG
TTAGCAGTTGCTAAAGTTGCAAGTATAATAGCACTGATTTATAGATATATAACCAATCAAGATTTTGTCGAATCTGATAC
AAAGTTATCTTACAGTGAAAATTTTGTACATATGATGTTTGATATTTCTTCTTATAAGTTCACTCAAGTTGTTGCTAAGG
CTTTGGATATTATTTTTGTATTACATGCTGATCATGAACAGAATGCTTCTACTGCTACTGTTAGATTATCTGGTTCTTCA
GGTGCAGATTTATTTGCTTGTTTAGTGGCGGGAACAGCGACTTTGTGGGGGCCAGCTCATGGTGGAGCAAATGAAGCTGT
GATTAATATGTTAATGGCAATTGAAAAGCCAAGTAATGTAAAGCAATTTATTCAAAAGGTAAAAGATGGTAACAAGAGTA
CTAGACTTATGGGGTTTGGTCATAGAGTATATAAGAATTATGACCCACGTGCTAAAATAATGTATAAAATTTGTAGAGAA
GTATTAAATAATTTAAATGTGAATGATCCATTATTAGAAATAGCATTAGAGTTAGAAAATATAGCATTACAAGATAGTTA
TTTTATTGATCGTAATTTGTATCCTAATGTAGATTTTTATTCCGGAATTGTTTTAAAAGCTATTGGAATCCCGGTTAGGA
TGTTTACTACATTATTTGCATTAGCTAGAACGTCTGGTTGGTCTGCTCAATGGTATGAGATGGTAGCAGAAGAAAATTAT
AAAATATGTCGTCCACGTCAGTTGTATACTGGTAATATTGCAGAATGTTAA

Upstream 100 bases:

>100_bases
TTACTTCTGATAAGATTTGAAGTGTTTGGTTTCTTATTTTTATCTGTTTATGTTATATAAGCAAGATATGTGTGGGGTTA
TTATTAATTTGGGTAGGATT

Downstream 100 bases:

>100_bases
CATAATTTTGAATTATCATTGCTTATTGTTTTAATATTATTGATAATTATGCGCATATTAATTGTGGATTTGTATATGTA
TATCTTGTTAACCATAAAAA

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MVKEAVLELDGQRIKLPVLYDTHGNKAVDITSLYKNTGILTYDPGFMSTAACESRITFIDGNKGILCYRGHDIVNLVNNN
KSFIEIVYLLFYGVLPGLEDLQNFISQISQEYNPPKIVHDVIKSFPRDSHPMSILMACFSALASCYHNQDNSADQLKCAK
LAVAKVASIIALIYRYITNQDFVESDTKLSYSENFVHMMFDISSYKFTQVVAKALDIIFVLHADHEQNASTATVRLSGSS
GADLFACLVAGTATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFGHRVYKNYDPRAKIMYKICRE
VLNNLNVNDPLLEIALELENIALQDSYFIDRNLYPNVDFYSGIVLKAIGIPVRMFTTLFALARTSGWSAQWYEMVAEENY
KICRPRQLYTGNIAEC

Sequences:

>Translated_416_residues
MVKEAVLELDGQRIKLPVLYDTHGNKAVDITSLYKNTGILTYDPGFMSTAACESRITFIDGNKGILCYRGHDIVNLVNNN
KSFIEIVYLLFYGVLPGLEDLQNFISQISQEYNPPKIVHDVIKSFPRDSHPMSILMACFSALASCYHNQDNSADQLKCAK
LAVAKVASIIALIYRYITNQDFVESDTKLSYSENFVHMMFDISSYKFTQVVAKALDIIFVLHADHEQNASTATVRLSGSS
GADLFACLVAGTATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFGHRVYKNYDPRAKIMYKICRE
VLNNLNVNDPLLEIALELENIALQDSYFIDRNLYPNVDFYSGIVLKAIGIPVRMFTTLFALARTSGWSAQWYEMVAEENY
KICRPRQLYTGNIAEC
>Mature_416_residues
MVKEAVLELDGQRIKLPVLYDTHGNKAVDITSLYKNTGILTYDPGFMSTAACESRITFIDGNKGILCYRGHDIVNLVNNN
KSFIEIVYLLFYGVLPGLEDLQNFISQISQEYNPPKIVHDVIKSFPRDSHPMSILMACFSALASCYHNQDNSADQLKCAK
LAVAKVASIIALIYRYITNQDFVESDTKLSYSENFVHMMFDISSYKFTQVVAKALDIIFVLHADHEQNASTATVRLSGSS
GADLFACLVAGTATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFGHRVYKNYDPRAKIMYKICRE
VLNNLNVNDPLLEIALELENIALQDSYFIDRNLYPNVDFYSGIVLKAIGIPVRMFTTLFALARTSGWSAQWYEMVAEENY
KICRPRQLYTGNIAEC

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=350, Percent_Identity=27.4285714285714, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1786939, Length=414, Percent_Identity=48.792270531401, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786527, Length=351, Percent_Identity=30.4843304843305, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17555174, Length=381, Percent_Identity=27.8215223097113, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=325, Percent_Identity=25.8461538461538, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325257, Length=364, Percent_Identity=25.8241758241758, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=321, Percent_Identity=24.9221183800623, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24640126, Length=386, Percent_Identity=25.9067357512953, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640124, Length=386, Percent_Identity=25.9067357512953, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356863, Length=400, Percent_Identity=25.5, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 46678; Mature: 46678

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEAVLELDGQRIKLPVLYDTHGNKAVDITSLYKNTGILTYDPGFMSTAACESRITFID
CCCHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECCCCCCHHHHCCEEEEEE
GNKGILCYRGHDIVNLVNNNKSFIEIVYLLFYGVLPGLEDLQNFISQISQEYNPPKIVHD
CCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
VIKSFPRDSHPMSILMACFSALASCYHNQDNSADQLKCAKLAVAKVASIIALIYRYITNQ
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DFVESDTKLSYSENFVHMMFDISSYKFTQVVAKALDIIFVLHADHEQNASTATVRLSGSS
CHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCC
GADLFACLVAGTATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFG
CHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECC
HRVYKNYDPRAKIMYKICREVLNNLNVNDPLLEIALELENIALQDSYFIDRNLYPNVDFY
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCEEECCCCCCCCCHH
SGIVLKAIGIPVRMFTTLFALARTSGWSAQWYEMVAEENYKICRPRQLYTGNIAEC
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MVKEAVLELDGQRIKLPVLYDTHGNKAVDITSLYKNTGILTYDPGFMSTAACESRITFID
CCCHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHCCCEEEECCCCCCHHHHCCEEEEEE
GNKGILCYRGHDIVNLVNNNKSFIEIVYLLFYGVLPGLEDLQNFISQISQEYNPPKIVHD
CCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
VIKSFPRDSHPMSILMACFSALASCYHNQDNSADQLKCAKLAVAKVASIIALIYRYITNQ
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DFVESDTKLSYSENFVHMMFDISSYKFTQVVAKALDIIFVLHADHEQNASTATVRLSGSS
CHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEEECCC
GADLFACLVAGTATLWGPAHGGANEAVINMLMAIEKPSNVKQFIQKVKDGNKSTRLMGFG
CHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECC
HRVYKNYDPRAKIMYKICREVLNNLNVNDPLLEIALELENIALQDSYFIDRNLYPNVDFY
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCEEECCCCCCCCCHH
SGIVLKAIGIPVRMFTTLFALARTSGWSAQWYEMVAEENYKICRPRQLYTGNIAEC
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112124; 9823893; 7557459 [H]