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The map label for this gene is nadA

Identifier: 88657840

GI number: 88657840

Start: 31156

End: 32100

Strand: Direct

Name: nadA

Synonym: ECH_0036

Alternate gene names: 88657840

Gene position: 31156-32100 (Clockwise)

Preceding gene: 88658129

Following gene: 88658043

Centisome position: 2.65

GC content: 31.85

Gene sequence:

>945_bases
ATGAAAGAACTTGATGTCATAACACTATTGCAAGAAATACGTCATTTGGCTCAGGAAAGTAATGCTGTAATTCTTGCTCA
TTACTATCAGGATTCAGAGATACAAGATATTGCTGATTTTATTGGTGATTCTTTAGAACTTTCCAGAAAGGCAGCCACCA
CAACAGCTGATGTTATTGTATTTTGTGGTGTGTACTTTATGGCTGAAGTTGCGAAAATAATTAATCCTGCCAAAAAGGTT
TTATTACCAGATTTAAATGCTGGATGTTCTTTAGCTGATTCTTGTGATGCAGAGAGTTTTAAAAAATTTCGTGAGTTACA
TAAAGATTGTGTATCAATTACATATATAAATTCTTTAGCGGAGGTTAAAGCTTATTCTGATATCATTTGTACATCTTCAA
GTGCTGAAAAGATAATAAGACAAATACCAGAAGAAAAACAAATTCTATTTGCTCCAGATAAATTTTTGGGAGCTTTTCTA
GAAAAGAAAACGAATAGGAAAATGATTTTATGGCCTGGTACTTGTATAGTGCATGAAAGTTTTTCTGAACGAGAGTTAAT
TGATATGATGGTTAGACATGATAAAGCATATGTTCTAGCTCATCCTGAATGTCCTGGGCATTTACTAAAATATGCTCATT
TCATTGGATCGACAACTCAGTTATTAAAATTTTCTTCTGATAATCCTAATTCAGAGTTTATAGTGTTGACTGAAGAAGGA
ATTATTCATCAAATGAAAAAAGTTTCTTCTGGAAGCACTTTTTATATAGTAAAAACTTCAGATAGTGGTGGATGTGTTTC
ATGTAGTAAATGCCCACATATGCGTTTGAATACTTTAGAAAAATTGTATTTATGTTTAAAAAATGGTTATCCTGAAATTA
CCCTTGATCCTGAAATTAGTAGTATGGCTAAAAGGTCTTTGGATGCCATGCTTAAAATGAGTTAA

Upstream 100 bases:

>100_bases
GATTTATAAGGAATTATATTCTTTGCAGAATTGTATTTTTAACTCGTGGAACATTATGTATTTGGTTGTTAAAAAGTAAT
TTTTTTGTCATAATGATTAA

Downstream 100 bases:

>100_bases
TCTTTGTTACGATATTGTTGATTTATTAATCTTATAGTCATAGTGATAATTATTTATAGAATAGCGATATGAATATGGCC
TATTATCTTTTCCTGGAGTA

Product: quinolinate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKELDVITLLQEIRHLAQESNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTADVIVFCGVYFMAEVAKIINPAKKV
LLPDLNAGCSLADSCDAESFKKFRELHKDCVSITYINSLAEVKAYSDIICTSSSAEKIIRQIPEEKQILFAPDKFLGAFL
EKKTNRKMILWPGTCIVHESFSERELIDMMVRHDKAYVLAHPECPGHLLKYAHFIGSTTQLLKFSSDNPNSEFIVLTEEG
IIHQMKKVSSGSTFYIVKTSDSGGCVSCSKCPHMRLNTLEKLYLCLKNGYPEITLDPEISSMAKRSLDAMLKMS

Sequences:

>Translated_314_residues
MKELDVITLLQEIRHLAQESNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTADVIVFCGVYFMAEVAKIINPAKKV
LLPDLNAGCSLADSCDAESFKKFRELHKDCVSITYINSLAEVKAYSDIICTSSSAEKIIRQIPEEKQILFAPDKFLGAFL
EKKTNRKMILWPGTCIVHESFSERELIDMMVRHDKAYVLAHPECPGHLLKYAHFIGSTTQLLKFSSDNPNSEFIVLTEEG
IIHQMKKVSSGSTFYIVKTSDSGGCVSCSKCPHMRLNTLEKLYLCLKNGYPEITLDPEISSMAKRSLDAMLKMS
>Mature_314_residues
MKELDVITLLQEIRHLAQESNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTADVIVFCGVYFMAEVAKIINPAKKV
LLPDLNAGCSLADSCDAESFKKFRELHKDCVSITYINSLAEVKAYSDIICTSSSAEKIIRQIPEEKQILFAPDKFLGAFL
EKKTNRKMILWPGTCIVHESFSERELIDMMVRHDKAYVLAHPECPGHLLKYAHFIGSTTQLLKFSSDNPNSEFIVLTEEG
IIHQMKKVSSGSTFYIVKTSDSGGCVSCSKCPHMRLNTLEKLYLCLKNGYPEITLDPEISSMAKRSLDAMLKMS

Specific function: Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate

COG id: COG0379

COG function: function code H; Quinolinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the quinolinate synthase A family. Type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786964, Length=312, Percent_Identity=35.5769230769231, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADA_EHRCR (O05104)

Other databases:

- EMBL:   U90899
- EMBL:   CP000236
- RefSeq:   YP_506869.1
- ProteinModelPortal:   O05104
- SMR:   O05104
- STRING:   O05104
- GeneID:   3927395
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0036
- TIGR:   ECH_0036
- eggNOG:   COG0379
- HOGENOM:   HBG291157
- OMA:   IAHPECE
- PhylomeDB:   O05104
- ProtClustDB:   PRK09375
- BioCyc:   ECHA205920:ECH_0036-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00568
- InterPro:   IPR003473
- InterPro:   IPR023066
- TIGRFAMs:   TIGR00550

Pfam domain/function: PF02445 NadA

EC number: =2.5.1.72

Molecular weight: Translated: 35178; Mature: 35178

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 44-44 BINDING 115-115 BINDING 132-132 BINDING 201-201 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDVITLLQEIRHLAQESNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTADVIV
CCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FCGVYFMAEVAKIINPAKKVLLPDLNAGCSLADSCDAESFKKFRELHKDCVSITYINSLA
HHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVKAYSDIICTSSSAEKIIRQIPEEKQILFAPDKFLGAFLEKKTNRKMILWPGTCIVHES
HHHHHHHHEECCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEECCEEEEECC
FSERELIDMMVRHDKAYVLAHPECPGHLLKYAHFIGSTTQLLKFSSDNPNSEFIVLTEEG
CCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCEEEEECCH
IIHQMKKVSSGSTFYIVKTSDSGGCVSCSKCPHMRLNTLEKLYLCLKNGYPEITLDPEIS
HHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHH
SMAKRSLDAMLKMS
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKELDVITLLQEIRHLAQESNAVILAHYYQDSEIQDIADFIGDSLELSRKAATTTADVIV
CCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FCGVYFMAEVAKIINPAKKVLLPDLNAGCSLADSCDAESFKKFRELHKDCVSITYINSLA
HHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVKAYSDIICTSSSAEKIIRQIPEEKQILFAPDKFLGAFLEKKTNRKMILWPGTCIVHES
HHHHHHHHEECCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEECCEEEEECC
FSERELIDMMVRHDKAYVLAHPECPGHLLKYAHFIGSTTQLLKFSSDNPNSEFIVLTEEG
CCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCEEEEECCH
IIHQMKKVSSGSTFYIVKTSDSGGCVSCSKCPHMRLNTLEKLYLCLKNGYPEITLDPEIS
HHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHH
SMAKRSLDAMLKMS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9297820