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The map label for this gene is thiF [H]

Identifier: 88657730

GI number: 88657730

Start: 1131165

End: 1131947

Strand: Reverse

Name: thiF [H]

Synonym: ECH_1107

Alternate gene names: 88657730

Gene position: 1131947-1131165 (Counterclockwise)

Preceding gene: 88657618

Following gene: 88658259

Centisome position: 96.23

GC content: 32.31

Gene sequence:

>783_bases
GTGTTTGACCGTTATAAGAAACAAGTTCTTATTTCAGAGATAGGCAAAGTAGGTCAAAGTAAATTAAGTGAAAGTAATGT
TTTGATAATAGGTTGTGGTGGCTTAGGAAGTACAGTTATTCCTCTACTTGCTGCTAGTGGTATTGGCCATATTACATTGT
GTGATGATGATAAAATACAAATGTCCAACCTTAATAGACAGGTGATTTATAAGGAGAGTGATATCAATCAAAGTAAAGTG
ATAAAAGCACAGGAGTTTGTAAAATCACTTAACTCTGATGTTAATGTTCAAGTGTTAAATAATTTTGTTACGCCTAAAAA
TTTCGAGGAAGTGTTTAAGAATGTGGATGTTGTTGTCGATTGTACAGATCGTCTGGCTACAAAGTTATTTCTTAATGATG
CAGCTGTTTTGTTAGGTAAACCTTTAGTACATAGTGCAGCGATTGGATTTACTGGACAAGTGTTAACAGTATTTCCTTAT
GGTAAACCTTGTCTAAGATGTTTTTTTGAATGTCAATATATGAGTCTGCACTTAAATTGTTCTAATGCTGGAATACTTGG
AGCAACGGTGGGAGTAGTAGGAAGTATTGCAGTTGCAGAAACTATAAAATATTTATTAAAAATTCCAGATAATTTAGTAG
GGAATTTGCAGAGAATAGACTTGCGATCTAATGAGTTTACTAAATATACATTTCAAAAAAATAGTGCATGTATAGCATGT
AGTGACAATATGAAAGTCGATCCTTATGATTATAATTATTATGAGAGCAAATTGTGTTTTTAG

Upstream 100 bases:

>100_bases
TTGAATTAAATATTAAAAGTTATGTAGATGAAGATGTTCCAGAATATTTGCGAAAGATACCTGTATCAGTTCCAGGTAGT
AGGTATTTACAGGAAAATTA

Downstream 100 bases:

>100_bases
ATTAATTACTTATTTGATGTGTATATCTATAAATGAAATAGGATGTAAGAGTTAAATAGCATTTAATATGTATTATTTTT
AATATAGAGGGTTTTATCCT

Product: adenylyltransferase thiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MFDRYKKQVLISEIGKVGQSKLSESNVLIIGCGGLGSTVIPLLAASGIGHITLCDDDKIQMSNLNRQVIYKESDINQSKV
IKAQEFVKSLNSDVNVQVLNNFVTPKNFEEVFKNVDVVVDCTDRLATKLFLNDAAVLLGKPLVHSAAIGFTGQVLTVFPY
GKPCLRCFFECQYMSLHLNCSNAGILGATVGVVGSIAVAETIKYLLKIPDNLVGNLQRIDLRSNEFTKYTFQKNSACIAC
SDNMKVDPYDYNYYESKLCF

Sequences:

>Translated_260_residues
MFDRYKKQVLISEIGKVGQSKLSESNVLIIGCGGLGSTVIPLLAASGIGHITLCDDDKIQMSNLNRQVIYKESDINQSKV
IKAQEFVKSLNSDVNVQVLNNFVTPKNFEEVFKNVDVVVDCTDRLATKLFLNDAAVLLGKPLVHSAAIGFTGQVLTVFPY
GKPCLRCFFECQYMSLHLNCSNAGILGATVGVVGSIAVAETIKYLLKIPDNLVGNLQRIDLRSNEFTKYTFQKNSACIAC
SDNMKVDPYDYNYYESKLCF
>Mature_260_residues
MFDRYKKQVLISEIGKVGQSKLSESNVLIIGCGGLGSTVIPLLAASGIGHITLCDDDKIQMSNLNRQVIYKESDINQSKV
IKAQEFVKSLNSDVNVQVLNNFVTPKNFEEVFKNVDVVVDCTDRLATKLFLNDAAVLLGKPLVHSAAIGFTGQVLTVFPY
GKPCLRCFFECQYMSLHLNCSNAGILGATVGVVGSIAVAETIKYLLKIPDNLVGNLQRIDLRSNEFTKYTFQKNSACIAC
SDNMKVDPYDYNYYESKLCF

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=255, Percent_Identity=36.4705882352941, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI23510338, Length=167, Percent_Identity=28.7425149700599, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI23510340, Length=167, Percent_Identity=28.7425149700599, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI4885649, Length=154, Percent_Identity=29.2207792207792, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI38045944, Length=166, Percent_Identity=30.7228915662651, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI38045942, Length=163, Percent_Identity=30.6748466257669, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI150417996, Length=168, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI13376212, Length=185, Percent_Identity=28.1081081081081, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI38327032, Length=185, Percent_Identity=28.1081081081081, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=35.8870967741936, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787048, Length=240, Percent_Identity=32.5, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789177, Length=111, Percent_Identity=30.6306306306306, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=253, Percent_Identity=40.7114624505929, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI193203301, Length=164, Percent_Identity=26.219512195122, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71984476, Length=162, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI86565433, Length=166, Percent_Identity=28.3132530120482, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=166, Percent_Identity=28.3132530120482, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=214, Percent_Identity=34.5794392523364, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=30.0613496932515, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322639, Length=170, Percent_Identity=28.2352941176471, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582879, Length=241, Percent_Identity=37.344398340249, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28573937, Length=166, Percent_Identity=30.7228915662651, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24660640, Length=150, Percent_Identity=31.3333333333333, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129973, Length=164, Percent_Identity=28.6585365853659, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28708; Mature: 28708

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDRYKKQVLISEIGKVGQSKLSESNVLIIGCGGLGSTVIPLLAASGIGHITLCDDDKIQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEE
MSNLNRQVIYKESDINQSKVIKAQEFVKSLNSDVNVQVLNNFVTPKNFEEVFKNVDVVVD
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
CTDRLATKLFLNDAAVLLGKPLVHSAAIGFTGQVLTVFPYGKPCLRCFFECQYMSLHLNC
CHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEEEEEEEC
SNAGILGATVGVVGSIAVAETIKYLLKIPDNLVGNLQRIDLRSNEFTKYTFQKNSACIAC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCEEEEEECCCEEEEE
SDNMKVDPYDYNYYESKLCF
CCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFDRYKKQVLISEIGKVGQSKLSESNVLIIGCGGLGSTVIPLLAASGIGHITLCDDDKIQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEE
MSNLNRQVIYKESDINQSKVIKAQEFVKSLNSDVNVQVLNNFVTPKNFEEVFKNVDVVVD
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
CTDRLATKLFLNDAAVLLGKPLVHSAAIGFTGQVLTVFPYGKPCLRCFFECQYMSLHLNC
CHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHEEEEEEEEEC
SNAGILGATVGVVGSIAVAETIKYLLKIPDNLVGNLQRIDLRSNEFTKYTFQKNSACIAC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCEEEEEECCCEEEEE
SDNMKVDPYDYNYYESKLCF
CCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]