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The map label for this gene is glyS [H]

Identifier: 88657718

GI number: 88657718

Start: 19647

End: 21758

Strand: Direct

Name: glyS [H]

Synonym: ECH_0024

Alternate gene names: 88657718

Gene position: 19647-21758 (Clockwise)

Preceding gene: 88658353

Following gene: 88657578

Centisome position: 1.67

GC content: 26.85

Gene sequence:

>2112_bases
ATGCCTTTAGAACTATTATTTGAATGCTTATCAGAAGAAACACCTCCTGAGATGCAGAGTAGTGCATATGCATACATGGA
TTCTTATATAAGAAAAGAACTAAGTCGTTGTAACATCAGTTTTGAGTCTGTAAAGGTATTTGTAACTTCAAATAGATTAA
CCTTGTATGTAAAAAATATAATCAATAACTTATCAAATAATACATTAAAGATAAAAGGACCTAAAGTATCAGCAGATAAG
ATTGCAATAGAAGGTTTTTTAAAGAAAGTTAATAAAAATTTCAGTGACCTTATAACATGCAAAATCGGTAATGATGATTT
TTACTATGCTGAGCTTAATCATAATGAAGAAACAAATACAGAACATATAATCCTATCAATTATAGAAAACATGTTAAACA
ACTTTCCATGGGATAAAAAAATGAGATGGGGAGGTGGAAAAACATATTGGGTTCGCCCTATACTAAATATAATGTGTATA
CTTGATGGAAAAGTACTACCAATAAAGTTTGCAGATATAGAAGCAAATAACAAATCATGTGGTAACAGATTCACAAATAA
AGAATTTTTTGAAGTTGCAGATTTTAATTCTTATAATTCAAAACTAAAAAAAAATAATGTAATTTTATGCCAAAAAGAAA
GGCTCAATTTTATATTACATCAAATAAAAAGTATAACAGAAGAAAGTAATTTAATATGTGAGGATAATCAAAAATTAATT
AATAAATTGAATGGAACTTTAGAATATCCAATAGTTATCATGGGATCAGTGGATGAAAAATTCTCTGAGTTACCAAAGGA
ATTAATACTAAGTGTGATGCACAATCATCAAAAATACCTAGCTGTTTTTAAAGAAGACAAAATTACAAACTTTATTACGA
TATCTACTATATCAAATGATAATATAATAAAAGGACACAATAACATATTAAATGCCAGGCTATTAGACGCAAGTTTTTTG
GTAAAAAAAGACAAAGAATACAATATTGATTACTACATAGAAAAACTCAAACAAATAGTATTTCATGCAAAACTTGGATC
TGTTTTTGAAAAAGTGAACCGCATAATAGCTTTATCAAAATACATTTCCATATGGATACCTCATTCCTCATTGATAAAGG
TAGAAAGAGCAGCAAAATTATCCAAGTTTGATTTATCAACACTAGCTATAAAAGAATTTCCAGAACTACAAGGAATAATG
GGAGGATATTATGCCTCTTACTTTAATGAATCATTAGAAATTTCAGAATCTATAGTTAATCACTACGAGCCAACAAACCC
ACAAAAAGAATGTCCTTCTTCTCCTATATCAATAGCTGTATCCATATCTGATAAAATAGATAACTTAGTTGGAATGATAG
TTGCAGGAGAGCAAGTTACCAGTTCCCGTGATCCTTTTTCCATGAGAAGAATGGCTATATCAATTATTAGAATAATAATC
GAAAACAATTTAAACATACCAATAAGGCTTTTAATAGAAAAATCTGTATCACTGTACTCATTTGCAATTACAGAAAGAAC
TACTATTAACAAACTTAAGAATGTAATATCAAGAACAAACAGAAAAAAAGAAATTATTTCATCTGTAATAGAATTTTTTT
CAAATAGATTTAAAATTATCCTAAAAGAAGACGGCATAAAGGAAGATATAATAAATGCCGTATTTGAAAGCAGAAAATTC
AGTAATTTATTGTTAATAAAAACAGAAGCTAAGTTTCTTAACAATTATATTTTTACCAAAAATGGGGTTGAAATAATAAA
GGCATATAAAAGACTTAGTAATATAATTACAAAGGGAGAAAACGTAAACAATACAGCAAAGTACAAGTGTAATAAAAAAT
TATTCACAATAAAAGAAGAAACTGATCTATATAAAGAAATAACTTACCAAAAAAATAATATAAAAAGATTGATAAAGGAC
AACAAGTTTGAGGAATCGTTAAATAATCTTTACAAATTGAGTTGTTGCGTAAACAGTTTTATGGACAACGTAAAAGTAAA
TGATCAAACAAATAAAGAATTACATAAAAACAGGCTTTCTATTGTTAAAAATGCATTTTATATTTTTGACTTAGTTACAG
ATTTTAGTAAGATTAAAAATACACATAGGTAA

Upstream 100 bases:

>100_bases
TGGAGTTAACGAACGTGCGTCTTATATATTACGCGTACGTAAACTTGCAAATTTATGTTGTAGCTTATATACTCAGTGTC
ATTAAAATAAGTAAAACGAC

Downstream 100 bases:

>100_bases
GTCTTGAATTTTTTTAAAATCGTATATAAATATTTTACCTGATACTTATAAAGAAGAATTGGTATGAGTAAGAGTGACTA
TTATGATCTATTAGGTGTAA

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MPLELLFECLSEETPPEMQSSAYAYMDSYIRKELSRCNISFESVKVFVTSNRLTLYVKNIINNLSNNTLKIKGPKVSADK
IAIEGFLKKVNKNFSDLITCKIGNDDFYYAELNHNEETNTEHIILSIIENMLNNFPWDKKMRWGGGKTYWVRPILNIMCI
LDGKVLPIKFADIEANNKSCGNRFTNKEFFEVADFNSYNSKLKKNNVILCQKERLNFILHQIKSITEESNLICEDNQKLI
NKLNGTLEYPIVIMGSVDEKFSELPKELILSVMHNHQKYLAVFKEDKITNFITISTISNDNIIKGHNNILNARLLDASFL
VKKDKEYNIDYYIEKLKQIVFHAKLGSVFEKVNRIIALSKYISIWIPHSSLIKVERAAKLSKFDLSTLAIKEFPELQGIM
GGYYASYFNESLEISESIVNHYEPTNPQKECPSSPISIAVSISDKIDNLVGMIVAGEQVTSSRDPFSMRRMAISIIRIII
ENNLNIPIRLLIEKSVSLYSFAITERTTINKLKNVISRTNRKKEIISSVIEFFSNRFKIILKEDGIKEDIINAVFESRKF
SNLLLIKTEAKFLNNYIFTKNGVEIIKAYKRLSNIITKGENVNNTAKYKCNKKLFTIKEETDLYKEITYQKNNIKRLIKD
NKFEESLNNLYKLSCCVNSFMDNVKVNDQTNKELHKNRLSIVKNAFYIFDLVTDFSKIKNTHR

Sequences:

>Translated_703_residues
MPLELLFECLSEETPPEMQSSAYAYMDSYIRKELSRCNISFESVKVFVTSNRLTLYVKNIINNLSNNTLKIKGPKVSADK
IAIEGFLKKVNKNFSDLITCKIGNDDFYYAELNHNEETNTEHIILSIIENMLNNFPWDKKMRWGGGKTYWVRPILNIMCI
LDGKVLPIKFADIEANNKSCGNRFTNKEFFEVADFNSYNSKLKKNNVILCQKERLNFILHQIKSITEESNLICEDNQKLI
NKLNGTLEYPIVIMGSVDEKFSELPKELILSVMHNHQKYLAVFKEDKITNFITISTISNDNIIKGHNNILNARLLDASFL
VKKDKEYNIDYYIEKLKQIVFHAKLGSVFEKVNRIIALSKYISIWIPHSSLIKVERAAKLSKFDLSTLAIKEFPELQGIM
GGYYASYFNESLEISESIVNHYEPTNPQKECPSSPISIAVSISDKIDNLVGMIVAGEQVTSSRDPFSMRRMAISIIRIII
ENNLNIPIRLLIEKSVSLYSFAITERTTINKLKNVISRTNRKKEIISSVIEFFSNRFKIILKEDGIKEDIINAVFESRKF
SNLLLIKTEAKFLNNYIFTKNGVEIIKAYKRLSNIITKGENVNNTAKYKCNKKLFTIKEETDLYKEITYQKNNIKRLIKD
NKFEESLNNLYKLSCCVNSFMDNVKVNDQTNKELHKNRLSIVKNAFYIFDLVTDFSKIKNTHR
>Mature_702_residues
PLELLFECLSEETPPEMQSSAYAYMDSYIRKELSRCNISFESVKVFVTSNRLTLYVKNIINNLSNNTLKIKGPKVSADKI
AIEGFLKKVNKNFSDLITCKIGNDDFYYAELNHNEETNTEHIILSIIENMLNNFPWDKKMRWGGGKTYWVRPILNIMCIL
DGKVLPIKFADIEANNKSCGNRFTNKEFFEVADFNSYNSKLKKNNVILCQKERLNFILHQIKSITEESNLICEDNQKLIN
KLNGTLEYPIVIMGSVDEKFSELPKELILSVMHNHQKYLAVFKEDKITNFITISTISNDNIIKGHNNILNARLLDASFLV
KKDKEYNIDYYIEKLKQIVFHAKLGSVFEKVNRIIALSKYISIWIPHSSLIKVERAAKLSKFDLSTLAIKEFPELQGIMG
GYYASYFNESLEISESIVNHYEPTNPQKECPSSPISIAVSISDKIDNLVGMIVAGEQVTSSRDPFSMRRMAISIIRIIIE
NNLNIPIRLLIEKSVSLYSFAITERTTINKLKNVISRTNRKKEIISSVIEFFSNRFKIILKEDGIKEDIINAVFESRKFS
NLLLIKTEAKFLNNYIFTKNGVEIIKAYKRLSNIITKGENVNNTAKYKCNKKLFTIKEETDLYKEITYQKNNIKRLIKDN
KFEESLNNLYKLSCCVNSFMDNVKVNDQTNKELHKNRLSIVKNAFYIFDLVTDFSKIKNTHR

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=712, Percent_Identity=26.2640449438202, Blast_Score=282, Evalue=4e-77,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 81280; Mature: 81149

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLELLFECLSEETPPEMQSSAYAYMDSYIRKELSRCNISFESVKVFVTSNRLTLYVKNI
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHEEEEEECCCEEEEHHHH
INNLSNNTLKIKGPKVSADKIAIEGFLKKVNKNFSDLITCKIGNDDFYYAELNHNEETNT
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCH
EHIILSIIENMLNNFPWDKKMRWGGGKTYWVRPILNIMCILDGKVLPIKFADIEANNKSC
HHHHHHHHHHHHHCCCCCCCEECCCCCEEEHHHHHHHHHHCCCCEEEEEEEEEECCCCCC
GNRFTNKEFFEVADFNSYNSKLKKNNVILCQKERLNFILHQIKSITEESNLICEDNQKLI
CCCCCCCHHHEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEECCCHHHH
NKLNGTLEYPIVIMGSVDEKFSELPKELILSVMHNHQKYLAVFKEDKITNFITISTISND
HHHCCCEECCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCEEEEEEEECCC
NIIKGHNNILNARLLDASFLVKKDKEYNIDYYIEKLKQIVFHAKLGSVFEKVNRIIALSK
CEEECCCCEEEHEEECHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YISIWIPHSSLIKVERAAKLSKFDLSTLAIKEFPELQGIMGGYYASYFNESLEISESIVN
HHEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHH
HYEPTNPQKECPSSPISIAVSISDKIDNLVGMIVAGEQVTSSRDPFSMRRMAISIIRIII
CCCCCCCHHHCCCCCCEEEEEECHHHHHHHHHEEECCHHCCCCCCHHHHHHHHHHHHHHH
ENNLNIPIRLLIEKSVSLYSFAITERTTINKLKNVISRTNRKKEIISSVIEFFSNRFKII
HCCCCCCEEEEEECCCCCEEEEHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
LKEDGIKEDIINAVFESRKFSNLLLIKTEAKFLNNYIFTKNGVEIIKAYKRLSNIITKGE
EECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCC
NVNNTAKYKCNKKLFTIKEETDLYKEITYQKNNIKRLIKDNKFEESLNNLYKLSCCVNSF
CCCCCCEEEECCEEEEEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MDNVKVNDQTNKELHKNRLSIVKNAFYIFDLVTDFSKIKNTHR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLELLFECLSEETPPEMQSSAYAYMDSYIRKELSRCNISFESVKVFVTSNRLTLYVKNI
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHEEEEEECCCEEEEHHHH
INNLSNNTLKIKGPKVSADKIAIEGFLKKVNKNFSDLITCKIGNDDFYYAELNHNEETNT
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCH
EHIILSIIENMLNNFPWDKKMRWGGGKTYWVRPILNIMCILDGKVLPIKFADIEANNKSC
HHHHHHHHHHHHHCCCCCCCEECCCCCEEEHHHHHHHHHHCCCCEEEEEEEEEECCCCCC
GNRFTNKEFFEVADFNSYNSKLKKNNVILCQKERLNFILHQIKSITEESNLICEDNQKLI
CCCCCCCHHHEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEECCCHHHH
NKLNGTLEYPIVIMGSVDEKFSELPKELILSVMHNHQKYLAVFKEDKITNFITISTISND
HHHCCCEECCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCEEEEEEEECCC
NIIKGHNNILNARLLDASFLVKKDKEYNIDYYIEKLKQIVFHAKLGSVFEKVNRIIALSK
CEEECCCCEEEHEEECHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YISIWIPHSSLIKVERAAKLSKFDLSTLAIKEFPELQGIMGGYYASYFNESLEISESIVN
HHEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHH
HYEPTNPQKECPSSPISIAVSISDKIDNLVGMIVAGEQVTSSRDPFSMRRMAISIIRIII
CCCCCCCHHHCCCCCCEEEEEECHHHHHHHHHEEECCHHCCCCCCHHHHHHHHHHHHHHH
ENNLNIPIRLLIEKSVSLYSFAITERTTINKLKNVISRTNRKKEIISSVIEFFSNRFKII
HCCCCCCEEEEEECCCCCEEEEHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEE
LKEDGIKEDIINAVFESRKFSNLLLIKTEAKFLNNYIFTKNGVEIIKAYKRLSNIITKGE
EECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCC
NVNNTAKYKCNKKLFTIKEETDLYKEITYQKNNIKRLIKDNKFEESLNNLYKLSCCVNSF
CCCCCCEEEECCEEEEEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MDNVKVNDQTNKELHKNRLSIVKNAFYIFDLVTDFSKIKNTHR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA