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The map label for this gene is nusA [H]

Identifier: 88657698

GI number: 88657698

Start: 567487

End: 569040

Strand: Direct

Name: nusA [H]

Synonym: ECH_0562

Alternate gene names: 88657698

Gene position: 567487-569040 (Clockwise)

Preceding gene: 88658157

Following gene: 88657990

Centisome position: 48.25

GC content: 32.43

Gene sequence:

>1554_bases
ATGGTTAATCTACAAAATTTTGATAACTTAGAGTTAATAAGGGTGGCTAAGGATATTGCTGATCAGAAAGGCTTGAATTT
GGATGTTATAATAAGAGCTATAGAGGAAGCTATCCAACTAACTAGTCGTAGTAGATATGGGAATTGTAAGATTAAAGTTA
CAGTGGATAAAAAAACTGGTGTAGTAGCTACTTATAGGCAAGTATTAGTTATCAATAATAATGGTGATATTAGTGTTCCT
GAAGGCAAAGAAAATGAGATAGATTTTTCTGATGTAAGCAAATATAAATTGATTACTTTAGCAGAAGCTAAACAAATTGA
TAAAAATGTTGAAGTTGGTGATATAATGCTTGAGCCTCTCCCTGTTATTGATCTTGATTATAGTTCTGCTAAGATTGCAA
AGCAAAAGATTGCTCAAGTTATTATTTCAGAAGAGCGTAAGAAACAGTATGAAGATTTTAAGGATCGTGTAGGTGATATT
GTTTATGGTACTGCTAAGCGTATAGAATATAATAATGTTATTGTAGATCTAAATGGAAATGAAGGTTATTTATCAGCTTC
AAATTTGATAAAAGGAGAGGTATTTCGTGTGGGTGATAGAGTAAAAGCTCACATAGAAGATGTAAGAAAGGAAAATTCAG
GACCTCAGATATTTCTTTCAAGAATCAATAAAGGATTCATGGAGCAATTATTCAAGCAGGAAATCCCAGAAATATATGAT
GGAATAGTTACAATTAAGGCAATAGCTAGGGATCCAGGTTCAAGGTCGAAGGTTGCGGTTTTTTCTTCTGATAAAAATAT
TGATCCAGTAGGTGCATGTGTTGGTGCAAGAGGTGTAAGAATTCAGGGTATTATTTCTGAATTACATGGAGAGAAGATTG
ATGTGATTTTATATTCTCCAGAACTTGCTAAATTTATAGTTAATGCTATAGCTCCTGCAGAAGTTTTAAAAGTCATTATT
GATGAAGATAAGGAAAAAGTAGAGTTAATTATTCCTGAAAATCAACTAAGTTTAGCAATTGGTAGGTATGGGCAGAATAT
CAGATTAGCATCTGAGTTGGTTGGTTGGAAAATAAATGTAATAGGAGATGAAACTGAATCAAGTAGAAAAGCGAAAGAAT
TGAGTGCAGGTTCAAAAATATTTGTTGAAGGATTGGATGTTGAAGAAATAATAGGGCAATTGTTATTTACTGAGGGGTTT
GTTAGTATTGAAGATATAGATCAAGCATCTGTTAGTGATATTTCTGCAATAGATGGGTTCAATGAGGAAATTGCAGAGGA
ATTAAAAAGTAGAGCAGCTGATTATTTAGTGCGTAAAGATGCTGAAATGAAACAGATTTTAGATAATTTATCTGTTGATA
AGGATGTTACTATGTTACCATTTTTAAGGCCGAGTGATATTATAGCGTTGTCGAAAAATGGTATCAACAGTCTTGAAGAT
ATTGCTGGATTGTGTACAGATGAGTTTTTTGATATTATTCCTGATATTGCATTGACTAAGGATCAAGTAGACTCTGTTAT
ATTAGAGTCTCGTAAGAAAATAGGATGGTTATAA

Upstream 100 bases:

>100_bases
TAAACTCCTACCGCGGTTTTATCTGAATATGTGTCATTTTTAAATAATCAGGTGCACTAAATCTTAGTTTTTTGTACATA
GGTTTTTACTAAGGGTTTTT

Downstream 100 bases:

>100_bases
CTTTTGTTTTTTGGGTAGTTGTCTATGAATGAGTCTAAAAGTGTTGTAGATAGTGGGTTGATGTCAGGTAAAATTGAACG
TACAACACTCAAGCTTAGTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVNLQNFDNLELIRVAKDIADQKGLNLDVIIRAIEEAIQLTSRSRYGNCKIKVTVDKKTGVVATYRQVLVINNNGDISVP
EGKENEIDFSDVSKYKLITLAEAKQIDKNVEVGDIMLEPLPVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRVGDI
VYGTAKRIEYNNVIVDLNGNEGYLSASNLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRINKGFMEQLFKQEIPEIYD
GIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQGIISELHGEKIDVILYSPELAKFIVNAIAPAEVLKVII
DEDKEKVELIIPENQLSLAIGRYGQNIRLASELVGWKINVIGDETESSRKAKELSAGSKIFVEGLDVEEIIGQLLFTEGF
VSIEDIDQASVSDISAIDGFNEEIAEELKSRAADYLVRKDAEMKQILDNLSVDKDVTMLPFLRPSDIIALSKNGINSLED
IAGLCTDEFFDIIPDIALTKDQVDSVILESRKKIGWL

Sequences:

>Translated_517_residues
MVNLQNFDNLELIRVAKDIADQKGLNLDVIIRAIEEAIQLTSRSRYGNCKIKVTVDKKTGVVATYRQVLVINNNGDISVP
EGKENEIDFSDVSKYKLITLAEAKQIDKNVEVGDIMLEPLPVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRVGDI
VYGTAKRIEYNNVIVDLNGNEGYLSASNLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRINKGFMEQLFKQEIPEIYD
GIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQGIISELHGEKIDVILYSPELAKFIVNAIAPAEVLKVII
DEDKEKVELIIPENQLSLAIGRYGQNIRLASELVGWKINVIGDETESSRKAKELSAGSKIFVEGLDVEEIIGQLLFTEGF
VSIEDIDQASVSDISAIDGFNEEIAEELKSRAADYLVRKDAEMKQILDNLSVDKDVTMLPFLRPSDIIALSKNGINSLED
IAGLCTDEFFDIIPDIALTKDQVDSVILESRKKIGWL
>Mature_517_residues
MVNLQNFDNLELIRVAKDIADQKGLNLDVIIRAIEEAIQLTSRSRYGNCKIKVTVDKKTGVVATYRQVLVINNNGDISVP
EGKENEIDFSDVSKYKLITLAEAKQIDKNVEVGDIMLEPLPVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRVGDI
VYGTAKRIEYNNVIVDLNGNEGYLSASNLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRINKGFMEQLFKQEIPEIYD
GIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQGIISELHGEKIDVILYSPELAKFIVNAIAPAEVLKVII
DEDKEKVELIIPENQLSLAIGRYGQNIRLASELVGWKINVIGDETESSRKAKELSAGSKIFVEGLDVEEIIGQLLFTEGF
VSIEDIDQASVSDISAIDGFNEEIAEELKSRAADYLVRKDAEMKQILDNLSVDKDVTMLPFLRPSDIIALSKNGINSLED
IAGLCTDEFFDIIPDIALTKDQVDSVILESRKKIGWL

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=512, Percent_Identity=33.0078125, Blast_Score=289, Evalue=4e-79,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 57429; Mature: 57429

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLQNFDNLELIRVAKDIADQKGLNLDVIIRAIEEAIQLTSRSRYGNCKIKVTVDKKTG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
VVATYRQVLVINNNGDISVPEGKENEIDFSDVSKYKLITLAEAKQIDKNVEVGDIMLEPL
EEEEEEEEEEEECCCCEECCCCCCCCCCHHHCCCEEEEEEHHHHHHCCCCCCCCEEECCC
PVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRVGDIVYGTAKRIEYNNVIVDLNGN
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHEEEECCEEEEECCC
EGYLSASNLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRINKGFMEQLFKQEIPEIYD
CCEEEHHHCCCCHHHEHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
GIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQGIISELHGEKIDVILYSP
CCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECH
ELAKFIVNAIAPAEVLKVIIDEDKEKVELIIPENQLSLAIGRYGQNIRLASELVGWKINV
HHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEHHCCCCEEEHHHHHCEEEEE
IGDETESSRKAKELSAGSKIFVEGLDVEEIIGQLLFTEGFVSIEDIDQASVSDISAIDGF
ECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEHHHCCCCHHHHHHHHCCC
NEEIAEELKSRAADYLVRKDAEMKQILDNLSVDKDVTMLPFLRPSDIIALSKNGINSLED
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCCCHHHH
IAGLCTDEFFDIIPDIALTKDQVDSVILESRKKIGWL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVNLQNFDNLELIRVAKDIADQKGLNLDVIIRAIEEAIQLTSRSRYGNCKIKVTVDKKTG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
VVATYRQVLVINNNGDISVPEGKENEIDFSDVSKYKLITLAEAKQIDKNVEVGDIMLEPL
EEEEEEEEEEEECCCCEECCCCCCCCCCHHHCCCEEEEEEHHHHHHCCCCCCCCEEECCC
PVIDLDYSSAKIAKQKIAQVIISEERKKQYEDFKDRVGDIVYGTAKRIEYNNVIVDLNGN
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHEEEECCEEEEECCC
EGYLSASNLIKGEVFRVGDRVKAHIEDVRKENSGPQIFLSRINKGFMEQLFKQEIPEIYD
CCEEEHHHCCCCHHHEHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHC
GIVTIKAIARDPGSRSKVAVFSSDKNIDPVGACVGARGVRIQGIISELHGEKIDVILYSP
CCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECH
ELAKFIVNAIAPAEVLKVIIDEDKEKVELIIPENQLSLAIGRYGQNIRLASELVGWKINV
HHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCEEEEEHHCCCCEEEHHHHHCEEEEE
IGDETESSRKAKELSAGSKIFVEGLDVEEIIGQLLFTEGFVSIEDIDQASVSDISAIDGF
ECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEHHHCCCCHHHHHHHHCCC
NEEIAEELKSRAADYLVRKDAEMKQILDNLSVDKDVTMLPFLRPSDIIALSKNGINSLED
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCCCHHHH
IAGLCTDEFFDIIPDIALTKDQVDSVILESRKKIGWL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]