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The map label for this gene is mraW

Identifier: 88657585

GI number: 88657585

Start: 544520

End: 545413

Strand: Direct

Name: mraW

Synonym: ECH_0542

Alternate gene names: 88657585

Gene position: 544520-545413 (Clockwise)

Preceding gene: 88657993

Following gene: 88658366

Centisome position: 46.29

GC content: 32.21

Gene sequence:

>894_bases
ATGCATACTCCAGTTTTATTGAACGAAATGTTAGACATATTAAATCCTCAAGATGGGAAAGTATATGTTGATGCAACGTT
TGGGGCAGGGGGATATACTAGAGCAATTTTGAAATCTGCAAATTGTCAGGTTTACGCTATTGATCAAGATGAATGTACTA
ATGTTTTCTATGAGAAACTGGTTAATGATTTTCCGGGCAAGGTACATTTTTGTGTTAGTAAATTCAGTAAAATAAAGCAA
ATATTGCATGGAGCTCAATTAAAAAAAGTTGATGGTATAGTATTTGATATAGGAGTTTCTTCTATGCAATTGGAAGATGC
TAGTCGTGGTTTTTCTTTTTCTAAAGATGGTCCTCTTGATATGAGGATGAGTACTTCTATAAGTAATGTTGATGCATCGG
TATTTGTGAATACGGCATTTGAAGAGGAGATAGCTAATGTTATATATCAGTATGGAGGTGAAAAGTATTCTCGTAAGATA
GCGAAAGCGATTGCTGAATCAAGAAAAAAGAAACCAATTAAAACTACGGGTGAACTTGCATCTATAGTAAGATCTGTAAT
CTCACGTAGTAAAAATCATAGTATTGATCCTGCAACAAGGACATTTCAAGCAATTAGAATATGGGTTAATAAAGAATTAG
AAGAATTAGAACAAGGCATAATTGATTCTGCTGATTTATTAAATCCAGGTGGAAAAATAATAGTAGTGTCTTTTCATTCT
TTGGAAGATCGAATTGTAAAGGTAATGTTTAAATCATTATGTAGTGGAAGTAGTGTAATTTCACCAATAGGATTTCAGTT
AATTAATAAAAAAGTGATAAGGCCAAGTTTTGAAGAAATATTGAATAATCCTAGGTCTAGGTCTGCAAAGCTGAGAGCTA
TATTAAAAATATAG

Upstream 100 bases:

>100_bases
TATGGCTATTAATTGAGTGACAGTATATTGAAATGCATAGTAAAACGCTGTCTTTTGTGTTAAAAGTGTAATACAATGTT
GTTGTTTAAAGAAATTATAC

Downstream 100 bases:

>100_bases
TGTGTACTTTAAATGAAAGTTTGAGATTTGTTATATATTAAATGGATCATATATATATGGTTCGGCATTGGCATTTTTTT
TATGTATATATTGAATTAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MHTPVLLNEMLDILNPQDGKVYVDATFGAGGYTRAILKSANCQVYAIDQDECTNVFYEKLVNDFPGKVHFCVSKFSKIKQ
ILHGAQLKKVDGIVFDIGVSSMQLEDASRGFSFSKDGPLDMRMSTSISNVDASVFVNTAFEEEIANVIYQYGGEKYSRKI
AKAIAESRKKKPIKTTGELASIVRSVISRSKNHSIDPATRTFQAIRIWVNKELEELEQGIIDSADLLNPGGKIIVVSFHS
LEDRIVKVMFKSLCSGSSVISPIGFQLINKKVIRPSFEEILNNPRSRSAKLRAILKI

Sequences:

>Translated_297_residues
MHTPVLLNEMLDILNPQDGKVYVDATFGAGGYTRAILKSANCQVYAIDQDECTNVFYEKLVNDFPGKVHFCVSKFSKIKQ
ILHGAQLKKVDGIVFDIGVSSMQLEDASRGFSFSKDGPLDMRMSTSISNVDASVFVNTAFEEEIANVIYQYGGEKYSRKI
AKAIAESRKKKPIKTTGELASIVRSVISRSKNHSIDPATRTFQAIRIWVNKELEELEQGIIDSADLLNPGGKIIVVSFHS
LEDRIVKVMFKSLCSGSSVISPIGFQLINKKVIRPSFEEILNNPRSRSAKLRAILKI
>Mature_297_residues
MHTPVLLNEMLDILNPQDGKVYVDATFGAGGYTRAILKSANCQVYAIDQDECTNVFYEKLVNDFPGKVHFCVSKFSKIKQ
ILHGAQLKKVDGIVFDIGVSSMQLEDASRGFSFSKDGPLDMRMSTSISNVDASVFVNTAFEEEIANVIYQYGGEKYSRKI
AKAIAESRKKKPIKTTGELASIVRSVISRSKNHSIDPATRTFQAIRIWVNKELEELEQGIIDSADLLNPGGKIIVVSFHS
LEDRIVKVMFKSLCSGSSVISPIGFQLINKKVIRPSFEEILNNPRSRSAKLRAILKI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=38.2789317507418, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=39.8936170212766, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI1786270, Length=307, Percent_Identity=40.3908794788274, Blast_Score=217, Evalue=6e-58,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=35.9516616314199, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=35.9516616314199, Blast_Score=195, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_EHRCR (Q2GGS8)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507355.1
- ProteinModelPortal:   Q2GGS8
- SMR:   Q2GGS8
- STRING:   Q2GGS8
- GeneID:   3927048
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0542
- TIGR:   ECH_0542
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q2GGS8
- ProtClustDB:   PRK00050
- BioCyc:   ECHA205920:ECH_0542-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32996; Mature: 32996

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: BINDING 48-48 BINDING 75-75 BINDING 96-96 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPVLLNEMLDILNPQDGKVYVDATFGAGGYTRAILKSANCQVYAIDQDECTNVFYEKL
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
VNDFPGKVHFCVSKFSKIKQILHGAQLKKVDGIVFDIGVSSMQLEDASRGFSFSKDGPLD
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCCCEEHHHHHCCCCCCCCCCCC
MRMSTSISNVDASVFVNTAFEEEIANVIYQYGGEKYSRKIAKAIAESRKKKPIKTTGELA
EEECCCCCCCCHHHEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
SIVRSVISRSKNHSIDPATRTFQAIRIWVNKELEELEQGIIDSADLLNPGGKIIVVSFHS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEHH
LEDRIVKVMFKSLCSGSSVISPIGFQLINKKVIRPSFEEILNNPRSRSAKLRAILKI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHTPVLLNEMLDILNPQDGKVYVDATFGAGGYTRAILKSANCQVYAIDQDECTNVFYEKL
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
VNDFPGKVHFCVSKFSKIKQILHGAQLKKVDGIVFDIGVSSMQLEDASRGFSFSKDGPLD
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCCCEEHHHHHCCCCCCCCCCCC
MRMSTSISNVDASVFVNTAFEEEIANVIYQYGGEKYSRKIAKAIAESRKKKPIKTTGELA
EEECCCCCCCCHHHEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
SIVRSVISRSKNHSIDPATRTFQAIRIWVNKELEELEQGIIDSADLLNPGGKIIVVSFHS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEHH
LEDRIVKVMFKSLCSGSSVISPIGFQLINKKVIRPSFEEILNNPRSRSAKLRAILKI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA