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The map label for this gene is dnaB

Identifier: 88608872

GI number: 88608872

Start: 481270

End: 482754

Strand: Direct

Name: dnaB

Synonym: NSE_0553

Alternate gene names: 88608872

Gene position: 481270-482754 (Clockwise)

Preceding gene: 88608818

Following gene: 88608527

Centisome position: 56.03

GC content: 40.07

Gene sequence:

>1485_bases
GTGCTTGGTTTGGGACCAAGAGGTCGGGGGTTCGAATCCCTCCACTCCGACACTTTTCTGCATTATCAAAAAGCGTTGAA
TAATTTTTCCGCCGAACTTCCTAACAATATTGAAGCAGAACAACTTATACTCGGTGCTCTACTCACTAATAATGATGCCT
GTGATGAGATAGAAGAGATTATTTCATCATCTTCTTTTTATGCTCCGGTACATAGAAAAATCTTTGAAGTTTTACTTCAC
CTTAGATCAAAAGAACTTGTTGCTAATCCCGTAACCCTGAAGAATTATCTTGAATCAAGCAATGAATTAAGCGCTATTGG
TGGTCAAGAATATCTACTCAATATAATTGAAAAAGCTAGTATTGTAGTGGACGTATCAAGTTTAGCACGTGTAATACAAG
AGCTTTACATAAGAAGGCAACTAATCGTTTTGGCGCAAAAAATGATTTCTGGGGCCACCGGCGAACATAGCAATATAACT
ACAAGCGAGCAGATAGAAGAAGCCGAACATTTTTTATTTCAACTTGCGAGCTCAGGTGAACTTGATTCATCCTGCTTACA
CATTTCTAAACCGATTGAGGTCGCGGTTGAACGAGCAAAAATTACTTTCAAAGCAAAGGCACCGTTTCAGAATACTTCCA
CTGGCTTCAGAGATATAGATAATCTTTTGGGCGGGCTGCAAAATTCAGACCTCTTAATAATCGCAGGACGTCCATCTATG
GGTAAAACATCTTTAGCCATAAGTATGGCCCTAAGGGTAACTAGAGAGCTACAAAAGCAAGGAATGTCTGCGTGCTTTTT
CTCCTTAGAAATGTCAGCTGATCAAATTGCTTCTAGAATGCTGTCTGTGCATTCTGGAGTCGACGCATTCAGCATTAGAA
CAGGTAAAAAGTTTTCTGAAGAATCACTAAAGAAAGTGATTGAAAGTAGCAAAGCATTGTCCGAACTACCCCTTTTTATA
GATGATACTGCTTCTGTATCAATCTCAGCGCTACGCACAAAACTACGCAGATTGCATAGAAAGGCAAAACTCGGGGCCAT
TTTCATAGATTACCTACAACTTTTACGAGGCTCTAAGGGTACAGAGTTCAACAGAGTACAAGAAGTCTCTGAAATCACTA
AGGGACTAAAGTTAATCGCTAAGGAACTTAATGTACCAGTTATTGCTCTCTCGCAGCTCTCCCGACTGGTTGAACAGAGA
GAAGACAAGCGTCCGCAACTTTCTGATTTAAGAGAGTCGGGCTCAATAGAACAAGATGCAGACGTTGTAATGTTTGTTTT
TCGCGAAGCCTACTACATGATGCGTAAACAACCTTTAAGTGATGATGAAAATTATGAGAATTGGCAATTCAAGATGGACG
AAGTAAGAAACAAAGCCGAGATAATAATAGCAAAACAAAGGAACGGCCCAGTTGGGACCGCCCTACTTTTTTTCGATCAG
TCAACTACAGTCTTTGATGATCTGTCCCTATTCTCAGATGATTAA

Upstream 100 bases:

>100_bases
GCGGGAGACAAAATTAGTGTGCGTGCTCTTGAATTGACTATAAACAAGCTTTGATTTAGAATCTCGAGCTGTGTGCGGAG
TGTAGCACAGCCTGGTTAGT

Downstream 100 bases:

>100_bases
TAGGCACTGTTGATACCCTTTTGAGGAAAGCGCAACAGTCAAGGGGATATCCATAAAGGAATAGTAAAATTTTAAGTAAA
TTCCTTTCTCATTATTTCTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MLGLGPRGRGFESLHSDTFLHYQKALNNFSAELPNNIEAEQLILGALLTNNDACDEIEEIISSSSFYAPVHRKIFEVLLH
LRSKELVANPVTLKNYLESSNELSAIGGQEYLLNIIEKASIVVDVSSLARVIQELYIRRQLIVLAQKMISGATGEHSNIT
TSEQIEEAEHFLFQLASSGELDSSCLHISKPIEVAVERAKITFKAKAPFQNTSTGFRDIDNLLGGLQNSDLLIIAGRPSM
GKTSLAISMALRVTRELQKQGMSACFFSLEMSADQIASRMLSVHSGVDAFSIRTGKKFSEESLKKVIESSKALSELPLFI
DDTASVSISALRTKLRRLHRKAKLGAIFIDYLQLLRGSKGTEFNRVQEVSEITKGLKLIAKELNVPVIALSQLSRLVEQR
EDKRPQLSDLRESGSIEQDADVVMFVFREAYYMMRKQPLSDDENYENWQFKMDEVRNKAEIIIAKQRNGPVGTALLFFDQ
STTVFDDLSLFSDD

Sequences:

>Translated_494_residues
MLGLGPRGRGFESLHSDTFLHYQKALNNFSAELPNNIEAEQLILGALLTNNDACDEIEEIISSSSFYAPVHRKIFEVLLH
LRSKELVANPVTLKNYLESSNELSAIGGQEYLLNIIEKASIVVDVSSLARVIQELYIRRQLIVLAQKMISGATGEHSNIT
TSEQIEEAEHFLFQLASSGELDSSCLHISKPIEVAVERAKITFKAKAPFQNTSTGFRDIDNLLGGLQNSDLLIIAGRPSM
GKTSLAISMALRVTRELQKQGMSACFFSLEMSADQIASRMLSVHSGVDAFSIRTGKKFSEESLKKVIESSKALSELPLFI
DDTASVSISALRTKLRRLHRKAKLGAIFIDYLQLLRGSKGTEFNRVQEVSEITKGLKLIAKELNVPVIALSQLSRLVEQR
EDKRPQLSDLRESGSIEQDADVVMFVFREAYYMMRKQPLSDDENYENWQFKMDEVRNKAEIIIAKQRNGPVGTALLFFDQ
STTVFDDLSLFSDD
>Mature_494_residues
MLGLGPRGRGFESLHSDTFLHYQKALNNFSAELPNNIEAEQLILGALLTNNDACDEIEEIISSSSFYAPVHRKIFEVLLH
LRSKELVANPVTLKNYLESSNELSAIGGQEYLLNIIEKASIVVDVSSLARVIQELYIRRQLIVLAQKMISGATGEHSNIT
TSEQIEEAEHFLFQLASSGELDSSCLHISKPIEVAVERAKITFKAKAPFQNTSTGFRDIDNLLGGLQNSDLLIIAGRPSM
GKTSLAISMALRVTRELQKQGMSACFFSLEMSADQIASRMLSVHSGVDAFSIRTGKKFSEESLKKVIESSKALSELPLFI
DDTASVSISALRTKLRRLHRKAKLGAIFIDYLQLLRGSKGTEFNRVQEVSEITKGLKLIAKELNVPVIALSQLSRLVEQR
EDKRPQLSDLRESGSIEQDADVVMFVFREAYYMMRKQPLSDDENYENWQFKMDEVRNKAEIIIAKQRNGPVGTALLFFDQ
STTVFDDLSLFSDD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=459, Percent_Identity=36.3834422657952, Blast_Score=271, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55207; Mature: 55207

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLGPRGRGFESLHSDTFLHYQKALNNFSAELPNNIEAEQLILGALLTNNDACDEIEEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
ISSSSFYAPVHRKIFEVLLHLRSKELVANPVTLKNYLESSNELSAIGGQEYLLNIIEKAS
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCCHHHHHHHHHHHH
IVVDVSSLARVIQELYIRRQLIVLAQKMISGATGEHSNITTSEQIEEAEHFLFQLASSGE
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
LDSSCLHISKPIEVAVERAKITFKAKAPFQNTSTGFRDIDNLLGGLQNSDLLIIAGRPSM
CCCHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
GKTSLAISMALRVTRELQKQGMSACFFSLEMSADQIASRMLSVHSGVDAFSIRTGKKFSE
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCH
ESLKKVIESSKALSELPLFIDDTASVSISALRTKLRRLHRKAKLGAIFIDYLQLLRGSKG
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TEFNRVQEVSEITKGLKLIAKELNVPVIALSQLSRLVEQREDKRPQLSDLRESGSIEQDA
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCH
DVVMFVFREAYYMMRKQPLSDDENYENWQFKMDEVRNKAEIIIAKQRNGPVGTALLFFDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHCCCCEEEEEEECCCCCCEEEEEEECC
STTVFDDLSLFSDD
CCCHHHHHHHHCCC
>Mature Secondary Structure
MLGLGPRGRGFESLHSDTFLHYQKALNNFSAELPNNIEAEQLILGALLTNNDACDEIEEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
ISSSSFYAPVHRKIFEVLLHLRSKELVANPVTLKNYLESSNELSAIGGQEYLLNIIEKAS
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCCHHHHHHHHHHHH
IVVDVSSLARVIQELYIRRQLIVLAQKMISGATGEHSNITTSEQIEEAEHFLFQLASSGE
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
LDSSCLHISKPIEVAVERAKITFKAKAPFQNTSTGFRDIDNLLGGLQNSDLLIIAGRPSM
CCCHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
GKTSLAISMALRVTRELQKQGMSACFFSLEMSADQIASRMLSVHSGVDAFSIRTGKKFSE
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCH
ESLKKVIESSKALSELPLFIDDTASVSISALRTKLRRLHRKAKLGAIFIDYLQLLRGSKG
HHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TEFNRVQEVSEITKGLKLIAKELNVPVIALSQLSRLVEQREDKRPQLSDLRESGSIEQDA
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCH
DVVMFVFREAYYMMRKQPLSDDENYENWQFKMDEVRNKAEIIIAKQRNGPVGTALLFFDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHCCCCEEEEEEECCCCCCEEEEEEECC
STTVFDDLSLFSDD
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA