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The map label for this gene is nth

Identifier: 88608856

GI number: 88608856

Start: 209356

End: 210006

Strand: Reverse

Name: nth

Synonym: NSE_0246

Alternate gene names: 88608856

Gene position: 210006-209356 (Counterclockwise)

Preceding gene: 88608529

Following gene: 88607959

Centisome position: 24.45

GC content: 37.94

Gene sequence:

>651_bases
ATGATGAAAAAAACACGTCCTTCACGTTCTTGGGTAGTTGAAATCCTGGAAAGGTTTCAGAGACAAATGCCGGAGCCTAA
AATCGAGTTAGAATATGTAAATAAATTCACGCTTATCATAGCAGTGCTACTATCCGCACAGTCCACAGATGTTAGTGTGA
ACAAAGCGACAAAGGCTCTATTTCGTGTTGCATACGAGCCAGAGCATTACGCAAAAATGGACCTTGCAAAACTGAAAGAG
TCTATAAAAACGATTGGTTTGCACAATAACAAGGCAAAAAATATCATCGCCTTAGCAAAGAAACTGATTTCGGATAAGCA
GACTGACATACCAAATAATTTTCAATATCTACAGTCCCTTCCAGGAATAGGAAGAAAGTCAGCGAACGTAATATTGTGTA
CACTCTTTGGAGAAAAAAGAATCGCTGTAGATACACATGTCTTCAGAGTAAGCAATAGAATAGGACTTGTGCATGCAAGA
AATGTCTTAGAAGTAGAGAAACAATTACTTGAAAACATTCCAAAAACCTTCCTACCACAAGCACATCTGTGGCTTGTCTT
GCACGGGAGATACATATGTAAAGCAAGGAAACCAGAGTGCAAAAACTGTATCATCAATGATTTATGCAACTTTAAGCTGG
GTATAAAATAA

Upstream 100 bases:

>100_bases
TCTTGTTTCACATACTCGAAAAGGTAGTGTGAAACCGATGACAGCAAAGCTGGTAGTGAAGCACACAGTTTCGTTGACGG
GTGTGAAGGCTCTGTAGCTC

Downstream 100 bases:

>100_bases
GCGCCAAATAAGGTAAATTTATTGTATGGAATCCTGCTTCAGATAGGGTGAACTCATCGCAGAAGATGGAGTAAATTTAG
GGGAGGAAGAAGATGTCTGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MMKKTRPSRSWVVEILERFQRQMPEPKIELEYVNKFTLIIAVLLSAQSTDVSVNKATKALFRVAYEPEHYAKMDLAKLKE
SIKTIGLHNNKAKNIIALAKKLISDKQTDIPNNFQYLQSLPGIGRKSANVILCTLFGEKRIAVDTHVFRVSNRIGLVHAR
NVLEVEKQLLENIPKTFLPQAHLWLVLHGRYICKARKPECKNCIINDLCNFKLGIK

Sequences:

>Translated_216_residues
MMKKTRPSRSWVVEILERFQRQMPEPKIELEYVNKFTLIIAVLLSAQSTDVSVNKATKALFRVAYEPEHYAKMDLAKLKE
SIKTIGLHNNKAKNIIALAKKLISDKQTDIPNNFQYLQSLPGIGRKSANVILCTLFGEKRIAVDTHVFRVSNRIGLVHAR
NVLEVEKQLLENIPKTFLPQAHLWLVLHGRYICKARKPECKNCIINDLCNFKLGIK
>Mature_216_residues
MMKKTRPSRSWVVEILERFQRQMPEPKIELEYVNKFTLIIAVLLSAQSTDVSVNKATKALFRVAYEPEHYAKMDLAKLKE
SIKTIGLHNNKAKNIIALAKKLISDKQTDIPNNFQYLQSLPGIGRKSANVILCTLFGEKRIAVDTHVFRVSNRIGLVHAR
NVLEVEKQLLENIPKTFLPQAHLWLVLHGRYICKARKPECKNCIINDLCNFKLGIK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=186, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1787920, Length=200, Percent_Identity=50, Blast_Score=204, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=33.1491712707182, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=29.0502793296089, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24776; Mature: 24776

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKTRPSRSWVVEILERFQRQMPEPKIELEYVNKFTLIIAVLLSAQSTDVSVNKATKAL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FRVAYEPEHYAKMDLAKLKESIKTIGLHNNKAKNIIALAKKLISDKQTDIPNNFQYLQSL
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
PGIGRKSANVILCTLFGEKRIAVDTHVFRVSNRIGLVHARNVLEVEKQLLENIPKTFLPQ
CCCCCCCCCEEEEEEECCCEEEEHHHHEEHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCC
AHLWLVLHGRYICKARKPECKNCIINDLCNFKLGIK
HHEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMKKTRPSRSWVVEILERFQRQMPEPKIELEYVNKFTLIIAVLLSAQSTDVSVNKATKAL
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FRVAYEPEHYAKMDLAKLKESIKTIGLHNNKAKNIIALAKKLISDKQTDIPNNFQYLQSL
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
PGIGRKSANVILCTLFGEKRIAVDTHVFRVSNRIGLVHARNVLEVEKQLLENIPKTFLPQ
CCCCCCCCCEEEEEEECCCEEEEHHHHEEHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCC
AHLWLVLHGRYICKARKPECKNCIINDLCNFKLGIK
HHEEEEEECCEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]