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The map label for this gene is pdhC

Identifier: 88608175

GI number: 88608175

Start: 837687

End: 838898

Strand: Direct

Name: pdhC

Synonym: NSE_0953

Alternate gene names: 88608175

Gene position: 837687-838898 (Clockwise)

Preceding gene: 88608329

Following gene: 88608846

Centisome position: 97.52

GC content: 43.56

Gene sequence:

>1212_bases
ATGCCAGTAAAGATACTAATGCCGGCACTATCACCAACAATGAAAGAGGGTACGCTCGCAAAATGGCTAGTGAGTGAGGG
TGAAAAGATAGAAGCAGGACAAGTAATAGCGGAGATAGAAACTGATAAAGCCACGATGGAATTTGAAGCTGTCGACGAGG
GAGTACTAGGAAAAATACTCATCCCGGCAAAAACCGCAGGCGTAAAGGTAAATCAGCCAATAGCCGTTCTGCTCGATGAT
GGTGAAGGCGAAAAAGAACTAAAGAAGTTTCTATCCACGATAGACAAACCCACTGTCACGGACAATAAAGCAGAAACATC
AGATGGAGATAAAATCAAAAATAATCCCTCCAGTCTGCCAGCTGATAAGCAACAGGGCAGGGTAATTGCAACTCCGCTTG
CAAGAAAGATTGCGTCCATCAATGGTATTGACTTATCACTTATCGGAAGTGGAAGTGGCCCTGACGGTAGGATCGTAAAA
AATGACTTGCTTAAGTTGCTTGATGACGCACCACAAGTTCAAATGCACGGACATTGCACCGAGACCAGTATACCAATTTC
TCCAATGCGGCGTGTAATAGCGCAAAGACTCGTAGAATCGAAGCAAAATGTTCCTCATTTTTACCTTTCCGTGACTTGCT
ATTTGCAGCATCTGCTATCGGCCAAAAAAAAATTCTACGACTGTTTGGAAACAAAAGTCACAGTCAATGATTTCGTTATA
AAGGCATGCGCTTTTGCTTTGGATAAGAATCCAGCAATGAATGTGTCGTGGGAAGGAGAATTTATTAGGCAGAATCAAAC
TATTGATATATCAGTTGCCGTTGCGATCCCGGACGGACTAATTACACCTATAGTCTTCTCAGCAGATAAACTATCGCTTT
CATCTATTTCAGATGAGGTTCGGGAATTAGTGGATAAAGCAAAAGCAGGCAGACTTCAACCACGGGAATTCCAGGGCGGA
TCTTTTACTGTTTCCAATCTCGGTATGTATGGCATAGATGAATTTACAGCGATAATTAATCCACCTCAGGCAGCAATCCT
TGCCGTAGGTGCAGCGCGAAAAGTTCCAACTGTTTCTGCGGATGCGGTCGTAGTTAGCGACGTCGTGACGCTGACATTAT
CTTGTGACCATAGGGTTATCGATGGAGCATTGGCAGCGAGGTTTATGCAATCGCTTAAAAAGGCAATAGAAGACCCTGTA
ATAATGTTGTGA

Upstream 100 bases:

>100_bases
ACCACTCATAAGTGCTCTAAAAACCCCATTAGCTCGCCAAAATTTTGACAACCTCCACAAAAAGATAGATAATTCCCGAT
GTTTTCAAAAGCGCGTCCTT

Downstream 100 bases:

>100_bases
TACCTCCTGTAACCACAGAACTTTTTAAGTGGAACGTTTGCAGCAAGATTTACGGAGTTGCTCGAAAAGTGATTGGGAAT
CGCGTAACATTATCATGCTA

Product: pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPVKILMPALSPTMKEGTLAKWLVSEGEKIEAGQVIAEIETDKATMEFEAVDEGVLGKILIPAKTAGVKVNQPIAVLLDD
GEGEKELKKFLSTIDKPTVTDNKAETSDGDKIKNNPSSLPADKQQGRVIATPLARKIASINGIDLSLIGSGSGPDGRIVK
NDLLKLLDDAPQVQMHGHCTETSIPISPMRRVIAQRLVESKQNVPHFYLSVTCYLQHLLSAKKKFYDCLETKVTVNDFVI
KACAFALDKNPAMNVSWEGEFIRQNQTIDISVAVAIPDGLITPIVFSADKLSLSSISDEVRELVDKAKAGRLQPREFQGG
SFTVSNLGMYGIDEFTAIINPPQAAILAVGAARKVPTVSADAVVVSDVVTLTLSCDHRVIDGALAARFMQSLKKAIEDPV
IML

Sequences:

>Translated_403_residues
MPVKILMPALSPTMKEGTLAKWLVSEGEKIEAGQVIAEIETDKATMEFEAVDEGVLGKILIPAKTAGVKVNQPIAVLLDD
GEGEKELKKFLSTIDKPTVTDNKAETSDGDKIKNNPSSLPADKQQGRVIATPLARKIASINGIDLSLIGSGSGPDGRIVK
NDLLKLLDDAPQVQMHGHCTETSIPISPMRRVIAQRLVESKQNVPHFYLSVTCYLQHLLSAKKKFYDCLETKVTVNDFVI
KACAFALDKNPAMNVSWEGEFIRQNQTIDISVAVAIPDGLITPIVFSADKLSLSSISDEVRELVDKAKAGRLQPREFQGG
SFTVSNLGMYGIDEFTAIINPPQAAILAVGAARKVPTVSADAVVVSDVVTLTLSCDHRVIDGALAARFMQSLKKAIEDPV
IML
>Mature_402_residues
PVKILMPALSPTMKEGTLAKWLVSEGEKIEAGQVIAEIETDKATMEFEAVDEGVLGKILIPAKTAGVKVNQPIAVLLDDG
EGEKELKKFLSTIDKPTVTDNKAETSDGDKIKNNPSSLPADKQQGRVIATPLARKIASINGIDLSLIGSGSGPDGRIVKN
DLLKLLDDAPQVQMHGHCTETSIPISPMRRVIAQRLVESKQNVPHFYLSVTCYLQHLLSAKKKFYDCLETKVTVNDFVIK
ACAFALDKNPAMNVSWEGEFIRQNQTIDISVAVAIPDGLITPIVFSADKLSLSSISDEVRELVDKAKAGRLQPREFQGGS
FTVSNLGMYGIDEFTAIINPPQAAILAVGAARKVPTVSADAVVVSDVVTLTLSCDHRVIDGALAARFMQSLKKAIEDPVI
ML

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=43.0875576036866, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=38.1898454746137, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=38.1898454746137, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI260898739, Length=155, Percent_Identity=50.3225806451613, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=24.8837209302326, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=28.3687943262411, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786305, Length=408, Percent_Identity=26.9607843137255, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=39.2201834862385, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17538894, Length=222, Percent_Identity=44.1441441441441, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=25.5220417633411, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=28.3950617283951, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=37.4439461883408, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=30.6372549019608, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321632, Length=190, Percent_Identity=32.1052631578947, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=42.2680412371134, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=42.2680412371134, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI18859875, Length=420, Percent_Identity=28.0952380952381, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=30.3418803418803, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43469; Mature: 43338

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKILMPALSPTMKEGTLAKWLVSEGEKIEAGQVIAEIETDKATMEFEAVDEGVLGKIL
CCCEEEECCCCCCCCCCHHHHHHHCCCCEECCCCEEEEEECCCCEEEEHHCCCCCCCEEE
IPAKTAGVKVNQPIAVLLDDGEGEKELKKFLSTIDKPTVTDNKAETSDGDKIKNNPSSLP
ECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADKQQGRVIATPLARKIASINGIDLSLIGSGSGPDGRIVKNDLLKLLDDAPQVQMHGHCT
CHHHCCEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCC
ETSIPISPMRRVIAQRLVESKQNVPHFYLSVTCYLQHLLSAKKKFYDCLETKVTVNDFVI
CCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KACAFALDKNPAMNVSWEGEFIRQNQTIDISVAVAIPDGLITPIVFSADKLSLSSISDEV
HHHHHHHCCCCCEEEEECCCEEECCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHH
RELVDKAKAGRLQPREFQGGSFTVSNLGMYGIDEFTAIINPPQAAILAVGAARKVPTVSA
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCCCHHHEEEECCCCCCCCCCC
DAVVVSDVVTLTLSCDHRVIDGALAARFMQSLKKAIEDPVIML
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure 
PVKILMPALSPTMKEGTLAKWLVSEGEKIEAGQVIAEIETDKATMEFEAVDEGVLGKIL
CCEEEECCCCCCCCCCHHHHHHHCCCCEECCCCEEEEEECCCCEEEEHHCCCCCCCEEE
IPAKTAGVKVNQPIAVLLDDGEGEKELKKFLSTIDKPTVTDNKAETSDGDKIKNNPSSLP
ECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADKQQGRVIATPLARKIASINGIDLSLIGSGSGPDGRIVKNDLLKLLDDAPQVQMHGHCT
CHHHCCEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCC
ETSIPISPMRRVIAQRLVESKQNVPHFYLSVTCYLQHLLSAKKKFYDCLETKVTVNDFVI
CCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KACAFALDKNPAMNVSWEGEFIRQNQTIDISVAVAIPDGLITPIVFSADKLSLSSISDEV
HHHHHHHCCCCCEEEEECCCEEECCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHH
RELVDKAKAGRLQPREFQGGSFTVSNLGMYGIDEFTAIINPPQAAILAVGAARKVPTVSA
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCCCHHHEEEECCCCCCCCCCC
DAVVVSDVVTLTLSCDHRVIDGALAARFMQSLKKAIEDPVIML
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]