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The map label for this gene is thiE [H]

Identifier: 88608790

GI number: 88608790

Start: 185240

End: 185884

Strand: Direct

Name: thiE [H]

Synonym: NSE_0220

Alternate gene names: 88608790

Gene position: 185240-185884 (Clockwise)

Preceding gene: 88608618

Following gene: 88608679

Centisome position: 21.56

GC content: 38.6

Gene sequence:

>645_bases
ATGCAATTGAGTAAGGCCTTTTATATCCTTTCGCCAAATAGGCAAATTACCACTGCTGAATATGCTGATCTAGCAAGACT
CTTTCGAAATTTTTCCACGTATGTGTATGCATTTCAGTTGCGCATCAAGGATAGGAATTTGTTAGAAAGGGAGATACCAC
GTCTTAGTGATCTTTGTCATGAGTATAAAATCCCCCTTATAGTCAATGATTTCATTGATTTGGCATTAAGGTTTGAGGCA
GACGGTGTTCATGTAGGTGTAGCAGACAATACTCTAGAGCAGTGTCGGCATCTCTTACCAAGCGGAAAAATCGTAGGTGT
AAGTTGTTATAATGATATTGATCGGGCAAAAAAGAATCTCTTCGCTGATTATGTGTCTTTTGGGTGTTTCTTCGAAAGTC
AGACGAAACCCAATCCTGCAGCTAAAGCGAGTTTAGCAACACTAAATAATTGGAAAAATATAGCTCCTAGAGTTCCTTGT
GTATGTATTGGCGGGATAAATGAGAAGAACTTTGTGCAACTTCTGAGAAATGGTGCCGATATCGTTGCTTTCTCGAGTTA
TTTATGGAGAGGAGAATCCCCATATGGTAAATTCGCAGCACTTATAGAAACAAGTAGACATTATGAAACTTATAGAAACA
AGTAG

Upstream 100 bases:

>100_bases
CGTATAAGGTGAGTATTAAGAAGATATAAACAACTTAAAATTGAATAATTTTATACGTCCACTCACGAGCTGTAGAATTT
GACAAATATTTTGCTTGCGT

Downstream 100 bases:

>100_bases
ACATTATGAAAAGTTGCTGTATGAGTGATGACTTTAAATTTGATTAGGATCTACTCTGAAAACAGCAAAAGTGTCGGCTT
TTGTACATAGATTTGCGTAA

Product: putative thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MQLSKAFYILSPNRQITTAEYADLARLFRNFSTYVYAFQLRIKDRNLLEREIPRLSDLCHEYKIPLIVNDFIDLALRFEA
DGVHVGVADNTLEQCRHLLPSGKIVGVSCYNDIDRAKKNLFADYVSFGCFFESQTKPNPAAKASLATLNNWKNIAPRVPC
VCIGGINEKNFVQLLRNGADIVAFSSYLWRGESPYGKFAALIETSRHYETYRNK

Sequences:

>Translated_214_residues
MQLSKAFYILSPNRQITTAEYADLARLFRNFSTYVYAFQLRIKDRNLLEREIPRLSDLCHEYKIPLIVNDFIDLALRFEA
DGVHVGVADNTLEQCRHLLPSGKIVGVSCYNDIDRAKKNLFADYVSFGCFFESQTKPNPAAKASLATLNNWKNIAPRVPC
VCIGGINEKNFVQLLRNGADIVAFSSYLWRGESPYGKFAALIETSRHYETYRNK
>Mature_214_residues
MQLSKAFYILSPNRQITTAEYADLARLFRNFSTYVYAFQLRIKDRNLLEREIPRLSDLCHEYKIPLIVNDFIDLALRFEA
DGVHVGVADNTLEQCRHLLPSGKIVGVSCYNDIDRAKKNLFADYVSFGCFFESQTKPNPAAKASLATLNNWKNIAPRVPC
VCIGGINEKNFVQLLRNGADIVAFSSYLWRGESPYGKFAALIETSRHYETYRNK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=170, Percent_Identity=28.8235294117647, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=160, Percent_Identity=31.875, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24444; Mature: 24444

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSKAFYILSPNRQITTAEYADLARLFRNFSTYVYAFQLRIKDRNLLEREIPRLSDLCH
CCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHH
EYKIPLIVNDFIDLALRFEADGVHVGVADNTLEQCRHLLPSGKIVGVSCYNDIDRAKKNL
HHCCCEEEHHHHHEEEEEECCCEEEECCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
FADYVSFGCFFESQTKPNPAAKASLATLNNWKNIAPRVPCVCIGGINEKNFVQLLRNGAD
HHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCC
IVAFSSYLWRGESPYGKFAALIETSRHYETYRNK
EEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLSKAFYILSPNRQITTAEYADLARLFRNFSTYVYAFQLRIKDRNLLEREIPRLSDLCH
CCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHH
EYKIPLIVNDFIDLALRFEADGVHVGVADNTLEQCRHLLPSGKIVGVSCYNDIDRAKKNL
HHCCCEEEHHHHHEEEEEECCCEEEECCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHH
FADYVSFGCFFESQTKPNPAAKASLATLNNWKNIAPRVPCVCIGGINEKNFVQLLRNGAD
HHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCC
IVAFSSYLWRGESPYGKFAALIETSRHYETYRNK
EEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA