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The map label for this gene is glyA

Identifier: 88608618

GI number: 88608618

Start: 183588

End: 184847

Strand: Direct

Name: glyA

Synonym: NSE_0218

Alternate gene names: 88608618

Gene position: 183588-184847 (Clockwise)

Preceding gene: 88608285

Following gene: 88608790

Centisome position: 21.37

GC content: 45.0

Gene sequence:

>1260_bases
ATGAGGGGGGGGGGAATAGTGTTTTTTAAATCTAGGATTTCTGCGGTTGATCCGGCTGTTGCTAGGATTATTGATGGTGA
AGTTAGTAGACAGCGTAAACATCTGCAGTTGATTGCTTCCGAGAACTTTGCTAGTGCTGCAGTCCTGGAGGCGCAGGGGT
CCGTGTTTACAAATAAATATGCGGAGGGTTATCCAGGCAAGCGCTACTATTGCGGATGTGAATATGCAGATCAGATCGAG
CGTCTTGCAATAGAAAGGGTCTGTAAGTTATTTGGGTGTTCTTATGCTAATGTTCAGCCGCACTCTGGTTCCCAGGCAAA
TCAGGCCGTATTTTTAGCGCTCCTAAACCCTGGTGATACGGTTCTTGGTTTTTCTCTCGCGTCAGGGGGACATCTTACTC
ATGGTGCAAGTGTCAACCTTTCCGGAAAATGGTTTAATGCGGTTCACTACAACGTCAGAAGAGATAATTTCGAGATAGAC
ATGGATGAGGTCCGTGATTTGGCAAAGAAGCATTCCCCAAGAATGATCATTGCGGGCGCGTCAGCATACTCGAAGTATAT
AGATTTTAAGAGCTTTCGTGAAATAGCAGACGAGGTTGGTGCTTATTTATTAGGTGATGTGGCACACTACGCTGGTTTGA
TAGCTGCAGGCGAGTATCCGTCTCCCTTCCCTTATGTAGACGTTATGACCTCTACAACACATAAAACTTTGCGTGGTCCG
CGTGGTGCAATCGTTCTTACAAATAGTGAAGAACTCATAAGGAAAATAAATTCTGCTATATTTCCAGGATTGCAGGGTGG
TCCTCAGATGCATGCAATAGCTGCTAGGGCTGTTGCTTTTGGTGAAGCTCTTACTACGGAGTTCAAAGAGTACATCAGGT
CTGTCATAAGGAATGCAAAAACTCTTGCAAACGTTTTAAGAGAGAGAGGTTTTGATGTCCTCTCTGGCGGTACTGATACC
CACATCGTTATGATTGACTTGAGGAAGCTTAATCTGAAAGGTAATGTGAGCGCCTTAAAACTTGAGAGTGCTGGGATAAT
CTGCAACAAGAATGCTATTCCATTTGATGAGGAGAAGCCATTTGTGACGTCTGGCTTACGATTCGGTAGTCCCGCTGAAA
CCACCCGTGGAATGCGCGAATTGGAATTCGCGCACATCGGAGGGCTAATTGCTGACTTACTTGAGGAAAAAATCAGCACA
GATAATGCAGCTGAAATGGTCTTGGACCTCACAAGCAAGTTCAACTTTTACAACGTGTAA

Upstream 100 bases:

>100_bases
AGAGATCTGACTGTATCAAGGTGTCTTGAAATAGTTGAGGCATTTGTAAGTAGTAAGTTTAGTTGTGAGGAGCGTCACAA
GAAAAGGATAGAAAAAATTG

Downstream 100 bases:

>100_bases
GGGTGCCAACGTCACGCGCTGCGATGCTTAATGAATTCTGCAAACGCCAAGATGCCTTTGCAAAATAGAGGACAGAGGGA
ACTGATATCTATGTCTCCTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MRGGGIVFFKSRISAVDPAVARIIDGEVSRQRKHLQLIASENFASAAVLEAQGSVFTNKYAEGYPGKRYYCGCEYADQIE
RLAIERVCKLFGCSYANVQPHSGSQANQAVFLALLNPGDTVLGFSLASGGHLTHGASVNLSGKWFNAVHYNVRRDNFEID
MDEVRDLAKKHSPRMIIAGASAYSKYIDFKSFREIADEVGAYLLGDVAHYAGLIAAGEYPSPFPYVDVMTSTTHKTLRGP
RGAIVLTNSEELIRKINSAIFPGLQGGPQMHAIAARAVAFGEALTTEFKEYIRSVIRNAKTLANVLRERGFDVLSGGTDT
HIVMIDLRKLNLKGNVSALKLESAGIICNKNAIPFDEEKPFVTSGLRFGSPAETTRGMRELEFAHIGGLIADLLEEKIST
DNAAEMVLDLTSKFNFYNV

Sequences:

>Translated_419_residues
MRGGGIVFFKSRISAVDPAVARIIDGEVSRQRKHLQLIASENFASAAVLEAQGSVFTNKYAEGYPGKRYYCGCEYADQIE
RLAIERVCKLFGCSYANVQPHSGSQANQAVFLALLNPGDTVLGFSLASGGHLTHGASVNLSGKWFNAVHYNVRRDNFEID
MDEVRDLAKKHSPRMIIAGASAYSKYIDFKSFREIADEVGAYLLGDVAHYAGLIAAGEYPSPFPYVDVMTSTTHKTLRGP
RGAIVLTNSEELIRKINSAIFPGLQGGPQMHAIAARAVAFGEALTTEFKEYIRSVIRNAKTLANVLRERGFDVLSGGTDT
HIVMIDLRKLNLKGNVSALKLESAGIICNKNAIPFDEEKPFVTSGLRFGSPAETTRGMRELEFAHIGGLIADLLEEKIST
DNAAEMVLDLTSKFNFYNV
>Mature_419_residues
MRGGGIVFFKSRISAVDPAVARIIDGEVSRQRKHLQLIASENFASAAVLEAQGSVFTNKYAEGYPGKRYYCGCEYADQIE
RLAIERVCKLFGCSYANVQPHSGSQANQAVFLALLNPGDTVLGFSLASGGHLTHGASVNLSGKWFNAVHYNVRRDNFEID
MDEVRDLAKKHSPRMIIAGASAYSKYIDFKSFREIADEVGAYLLGDVAHYAGLIAAGEYPSPFPYVDVMTSTTHKTLRGP
RGAIVLTNSEELIRKINSAIFPGLQGGPQMHAIAARAVAFGEALTTEFKEYIRSVIRNAKTLANVLRERGFDVLSGGTDT
HIVMIDLRKLNLKGNVSALKLESAGIICNKNAIPFDEEKPFVTSGLRFGSPAETTRGMRELEFAHIGGLIADLLEEKIST
DNAAEMVLDLTSKFNFYNV

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=410, Percent_Identity=43.6585365853659, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI261862350, Length=410, Percent_Identity=43.6585365853659, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI261862348, Length=410, Percent_Identity=43.6585365853659, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI19923315, Length=410, Percent_Identity=43.6585365853659, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI261862346, Length=410, Percent_Identity=43.4146341463415, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI22547186, Length=400, Percent_Identity=44.75, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI22547189, Length=383, Percent_Identity=41.77545691906, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=52.4390243902439, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=44.75, Blast_Score=339, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=44.75, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=428, Percent_Identity=41.8224299065421, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=42.3357664233577, Blast_Score=315, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24640005, Length=405, Percent_Identity=44.4444444444444, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI221329721, Length=405, Percent_Identity=44.4444444444444, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_NEOSM (Q2GEI3)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506113.1
- HSSP:   P34897
- ProteinModelPortal:   Q2GEI3
- SMR:   Q2GEI3
- STRING:   Q2GEI3
- GeneID:   3932003
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0218
- TIGR:   NSE_0218
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   YGGCANV
- ProtClustDB:   CLSK2528038
- BioCyc:   NSEN222891:NSE_0218-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45772; Mature: 45772

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGGIVFFKSRISAVDPAVARIIDGEVSRQRKHLQLIASENFASAAVLEAQGSVFTNKY
CCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHH
AEGYPGKRYYCGCEYADQIERLAIERVCKLFGCSYANVQPHSGSQANQAVFLALLNPGDT
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
VLGFSLASGGHLTHGASVNLSGKWFNAVHYNVRRDNFEIDMDEVRDLAKKHSPRMIIAGA
EEEEEECCCCCCCCCCEEECCCCEEEEEEEEEECCCCEECHHHHHHHHHHCCCCEEEEEC
SAYSKYIDFKSFREIADEVGAYLLGDVAHYAGLIAAGEYPSPFPYVDVMTSTTHKTLRGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCHHHHHCCC
RGAIVLTNSEELIRKINSAIFPGLQGGPQMHAIAARAVAFGEALTTEFKEYIRSVIRNAK
CCEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLANVLRERGFDVLSGGTDTHIVMIDLRKLNLKGNVSALKLESAGIICNKNAIPFDEEKP
HHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCC
FVTSGLRFGSPAETTRGMRELEFAHIGGLIADLLEEKISTDNAAEMVLDLTSKFNFYNV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRGGGIVFFKSRISAVDPAVARIIDGEVSRQRKHLQLIASENFASAAVLEAQGSVFTNKY
CCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHH
AEGYPGKRYYCGCEYADQIERLAIERVCKLFGCSYANVQPHSGSQANQAVFLALLNPGDT
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
VLGFSLASGGHLTHGASVNLSGKWFNAVHYNVRRDNFEIDMDEVRDLAKKHSPRMIIAGA
EEEEEECCCCCCCCCCEEECCCCEEEEEEEEEECCCCEECHHHHHHHHHHCCCCEEEEEC
SAYSKYIDFKSFREIADEVGAYLLGDVAHYAGLIAAGEYPSPFPYVDVMTSTTHKTLRGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCHHHHHCCC
RGAIVLTNSEELIRKINSAIFPGLQGGPQMHAIAARAVAFGEALTTEFKEYIRSVIRNAK
CCEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLANVLRERGFDVLSGGTDTHIVMIDLRKLNLKGNVSALKLESAGIICNKNAIPFDEEKP
HHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCC
FVTSGLRFGSPAETTRGMRELEFAHIGGLIADLLEEKISTDNAAEMVLDLTSKFNFYNV
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA