Click here to switch to the map view.

The map label for this gene is proS

Identifier: 88608761

GI number: 88608761

Start: 300119

End: 301429

Strand: Direct

Name: proS

Synonym: NSE_0367

Alternate gene names: 88608761

Gene position: 300119-301429 (Clockwise)

Preceding gene: 88608143

Following gene: 88608547

Centisome position: 34.94

GC content: 42.72

Gene sequence:

>1311_bases
ATGAAAAATAAAACAAGAGTATCCGAGTACTATTTACCCCTTATGAAAAATGTATCGGGTGAGGTGAAGTTGCGCTCGCA
CAAGTATTCCCTGCGTGCCGGCCTTGTAAAGCAGTGTGGGGCCGGTCTATATAGTTGGCTGCCTTTGGGTCTCAAGGTGC
TAAGAAATATCGAGAGTGTAATCAGGCAAGAGCTTGATTCTATGGGAATGCATGAAATGCTAATGCCCTGCATTCAGTTG
GCTAAACTCTGGGAAGAGTCGGGAAGGTATTCGGACTATGGTAAGGAGCTGTTAAAGTTTAAGGATAGACATGACAACGA
GCTTCTTTTTGGTCCAACAAATGAGGAAGTCATTACAGCAATAGTTAGGGATGATCTTGCTAGCTATAAGCAGCTGCCTA
AGATTCTTTACCATATTCAGTGGAAGTTTAGGGATGAAATCAGGCCAAGATTTGGCCTTATGCGTGCCCGTGAGTTTCTT
ATGAAGGATGCCTACAGTTTTGATGTAGATCAGCAATCTGCTAGAATTTCTTATAACAAGGTCTATCAGTCCTATTTGCG
CATTTTTAAGAGACTTGGGCTCAATCCAATCCCTTGTAGAGCGAATGCTGGAGTTATAGGCGGCAGTCTGAATCACGAGT
TCCATATTACTACGACTGAAGGAGGGGAGGGTAAGATTTTTTATCCCGAGGAGATGGGAGAGCTCGTAGATGAATTTTGC
AAAATTGATCCTTCAAATGAGCACGCGGTATCTGTTATGACAGAGAAATTACAGGAGCTGTTTTGTTTCACCGAGGAATG
TAATAGCTCTGGTATAGGAAATGACAACAGAATCTGCACAGCGCAAGGAATAGAAGTGGGGCATATTTTCCTATTTGGGG
AGAAGTATTCTGCTCCTATGCAAGCGCGCTTCTCTGGTAGAGATGGAAAAAAGAAGAATTTTTACATGGGATCCTACGGT
ATTGGAATTTCGAGATTACTTGCTGCTATAATCGAAGTACACTCGGATGATAAGGGCATTATTTGGCCAGAGTCGATTGC
GCCATTTAAAATTGGGCTTATCAATTTGCACGGGGAATCTTGCGGTTTTGCTGAGGAGATTTTTTCCAAATTAGACAGCG
TCATCTATGATGATACGGCTGATAGTCAGGGTGTGAAGTTTGCCAGGATGGATTTAATTGGGGTGCCTTTCCAAATAATT
GTTGGTAAGAACGGTCCCATCCATGGAACAATTGAGCTGAAATATCGTCTTGACTCAAAAAGAGAAAGCCGCAGCCTCAA
CGAGCTTGTTTCACTTTTTAGCCATACGTAA

Upstream 100 bases:

>100_bases
ACAGTAAAAATCGTGATGAAATTTTTGTAGCGAAGCCTTTTGGGATTAATGAGTTAATTTCTAAAATCATAAGCTTAACC
GAAGGTGGGGGTTAGAATGC

Downstream 100 bases:

>100_bases
CAGGCATATTTGCGGGAGACAATCTTGTGAGTACATCTGAAAGGGAAGAAATACAGCTTTTGCAAGTGTCTGTCCGACTA
CTGGAGAGGCAGAATGCGCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKNKTRVSEYYLPLMKNVSGEVKLRSHKYSLRAGLVKQCGAGLYSWLPLGLKVLRNIESVIRQELDSMGMHEMLMPCIQL
AKLWEESGRYSDYGKELLKFKDRHDNELLFGPTNEEVITAIVRDDLASYKQLPKILYHIQWKFRDEIRPRFGLMRAREFL
MKDAYSFDVDQQSARISYNKVYQSYLRIFKRLGLNPIPCRANAGVIGGSLNHEFHITTTEGGEGKIFYPEEMGELVDEFC
KIDPSNEHAVSVMTEKLQELFCFTEECNSSGIGNDNRICTAQGIEVGHIFLFGEKYSAPMQARFSGRDGKKKNFYMGSYG
IGISRLLAAIIEVHSDDKGIIWPESIAPFKIGLINLHGESCGFAEEIFSKLDSVIYDDTADSQGVKFARMDLIGVPFQII
VGKNGPIHGTIELKYRLDSKRESRSLNELVSLFSHT

Sequences:

>Translated_436_residues
MKNKTRVSEYYLPLMKNVSGEVKLRSHKYSLRAGLVKQCGAGLYSWLPLGLKVLRNIESVIRQELDSMGMHEMLMPCIQL
AKLWEESGRYSDYGKELLKFKDRHDNELLFGPTNEEVITAIVRDDLASYKQLPKILYHIQWKFRDEIRPRFGLMRAREFL
MKDAYSFDVDQQSARISYNKVYQSYLRIFKRLGLNPIPCRANAGVIGGSLNHEFHITTTEGGEGKIFYPEEMGELVDEFC
KIDPSNEHAVSVMTEKLQELFCFTEECNSSGIGNDNRICTAQGIEVGHIFLFGEKYSAPMQARFSGRDGKKKNFYMGSYG
IGISRLLAAIIEVHSDDKGIIWPESIAPFKIGLINLHGESCGFAEEIFSKLDSVIYDDTADSQGVKFARMDLIGVPFQII
VGKNGPIHGTIELKYRLDSKRESRSLNELVSLFSHT
>Mature_436_residues
MKNKTRVSEYYLPLMKNVSGEVKLRSHKYSLRAGLVKQCGAGLYSWLPLGLKVLRNIESVIRQELDSMGMHEMLMPCIQL
AKLWEESGRYSDYGKELLKFKDRHDNELLFGPTNEEVITAIVRDDLASYKQLPKILYHIQWKFRDEIRPRFGLMRAREFL
MKDAYSFDVDQQSARISYNKVYQSYLRIFKRLGLNPIPCRANAGVIGGSLNHEFHITTTEGGEGKIFYPEEMGELVDEFC
KIDPSNEHAVSVMTEKLQELFCFTEECNSSGIGNDNRICTAQGIEVGHIFLFGEKYSAPMQARFSGRDGKKKNFYMGSYG
IGISRLLAAIIEVHSDDKGIIWPESIAPFKIGLINLHGESCGFAEEIFSKLDSVIYDDTADSQGVKFARMDLIGVPFQII
VGKNGPIHGTIELKYRLDSKRESRSLNELVSLFSHT

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=415, Percent_Identity=33.9759036144578, Blast_Score=238, Evalue=7e-63,
Organism=Escherichia coli, GI1786392, Length=222, Percent_Identity=47.7477477477478, Blast_Score=244, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI115532348, Length=393, Percent_Identity=32.3155216284987, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320931, Length=204, Percent_Identity=38.2352941176471, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656200, Length=400, Percent_Identity=32, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NEOSM (Q2GE40)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506256.1
- ProteinModelPortal:   Q2GE40
- SMR:   Q2GE40
- STRING:   Q2GE40
- GeneID:   3931764
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0367
- TIGR:   NSE_0367
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q2GE40
- ProtClustDB:   PRK12325
- BioCyc:   NSEN222891:NSE_0367-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49608; Mature: 49608

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKTRVSEYYLPLMKNVSGEVKLRSHKYSLRAGLVKQCGAGLYSWLPLGLKVLRNIESV
CCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IRQELDSMGMHEMLMPCIQLAKLWEESGRYSDYGKELLKFKDRHDNELLFGPTNEEVITA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHH
IVRDDLASYKQLPKILYHIQWKFRDEIRPRFGLMRAREFLMKDAYSFDVDQQSARISYNK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VYQSYLRIFKRLGLNPIPCRANAGVIGGSLNHEFHITTTEGGEGKIFYPEEMGELVDEFC
HHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHH
KIDPSNEHAVSVMTEKLQELFCFTEECNSSGIGNDNRICTAQGIEVGHIFLFGEKYSAPM
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCC
QARFSGRDGKKKNFYMGSYGIGISRLLAAIIEVHSDDKGIIWPESIAPFKIGLINLHGES
CEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCC
CGFAEEIFSKLDSVIYDDTADSQGVKFARMDLIGVPFQIIVGKNGPIHGTIELKYRLDSK
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHEECCCEEEEECCCCCEEEEEEEEEEECCC
RESRSLNELVSLFSHT
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNKTRVSEYYLPLMKNVSGEVKLRSHKYSLRAGLVKQCGAGLYSWLPLGLKVLRNIESV
CCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IRQELDSMGMHEMLMPCIQLAKLWEESGRYSDYGKELLKFKDRHDNELLFGPTNEEVITA
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHH
IVRDDLASYKQLPKILYHIQWKFRDEIRPRFGLMRAREFLMKDAYSFDVDQQSARISYNK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VYQSYLRIFKRLGLNPIPCRANAGVIGGSLNHEFHITTTEGGEGKIFYPEEMGELVDEFC
HHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHH
KIDPSNEHAVSVMTEKLQELFCFTEECNSSGIGNDNRICTAQGIEVGHIFLFGEKYSAPM
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCC
QARFSGRDGKKKNFYMGSYGIGISRLLAAIIEVHSDDKGIIWPESIAPFKIGLINLHGES
CEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCC
CGFAEEIFSKLDSVIYDDTADSQGVKFARMDLIGVPFQIIVGKNGPIHGTIELKYRLDSK
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHEECCCEEEEECCCCCEEEEEEEEEEECCC
RESRSLNELVSLFSHT
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA