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The map label for this gene is dnaX [H]

Identifier: 88608682

GI number: 88608682

Start: 405136

End: 406326

Strand: Reverse

Name: dnaX [H]

Synonym: NSE_0492

Alternate gene names: 88608682

Gene position: 406326-405136 (Counterclockwise)

Preceding gene: 88608653

Following gene: 88608781

Centisome position: 47.3

GC content: 40.05

Gene sequence:

>1191_bases
ATGAGTTATATAGGGCTTGCAAACAAATATAGGCCACGCCTACTCGATGAAATTGTTGGGCAGGATTTTCTGGTCAAGTG
CCTTTCCGCATCAATAGCAAGGGATGAGGTTGCTGGTGCTATCTTGCTATCAGGTCCATATGGAACAGGCAAGACAACAA
CAGCAAGAGCAGTAACTCTTTCTCTGCTGTGCTTAAACCGGACGAGCGCTTTGCCTTGTTTAAAATGTGATTCTTGCATC
TCAATACTGAGTGGATCCCATCCAGACATTCTAGAAATTGATGCAGCAAGCAATACAGGGGTTGAAGATGTTCGAATGTT
AATAGAGGGTGCGCTTTACAAGCCACTCCTCTCGAAATACAAGAGTTATATAGTTGATGAAGTTCATATGCTTTCTCAAA
GTGCCTTTAGCGCACTGTTGAAGCTACTTGAAGAACCACCGCAACATGTAAAGTTTTTCCTTGCAACCACGGAACTGCAA
AAGATTCCTGCGACTATAATCTCGAGATGCCAACATTATCGACTTTCGAGGTTAAGTAGGGTCGTAATCGCGGAAAGACT
CACCCAAGTTGCTGAGAAAGAACAAATTGAAATCACTCAAGATGCTATAAAATTCATTGCAGAAAAAGCGGATGGCTCGC
TTAGAGATGCTTTATCTCTTCTGGAACAAGTTACTCTTCATCCTTCCGAAGGAAAAATTACAGTACATGTCTTGAGAAAC
AAAATGGGTCTCGTGGACCTATCTGAAGTATACATGCTATTGGACTCCATCATTGCGGGTGATTGCAAAACCTCGCTTGG
TAAATTAAACGATTTATACTCCAAAGGCTACGAGATGCTTACAATTTTTATGGAGCTTGTAGCACTAATAAATGAAAAGA
TCCAGGATAAACTCACCGCTGAAGATAGCAGCAAGTCCGGATTTTTTTGTAGACTTGCACAATGCATGCTTTCCAGTATT
GAGGAAATTAAATCTTTTAACTGTGATCACAGTGTTGCTGAGCTCGCTATAATCAGAATGTCATACATAGCCAACTTACC
TTCTCCAGGAGAAATTATAAAGATTTTTTCAAATGAAATGCGTACAATACTTCCTAGAAATTCTGAAATATCTAAGAACA
GCAGTATGAGTTCTGCTGAGTTGACCAGTTATATACTTAAGGAGTTTGATGGTTCAGAATTATCAGACTAA

Upstream 100 bases:

>100_bases
GGCTGAGCCCAATTTTGTGGACTTAGGTGGAAAGTCTGCAGCTGTTGCGTGGACATCGTTTTTCCGTGTATGGTTTGTAC
TGAGTCTATCTCGCATTAGC

Downstream 100 bases:

>100_bases
TAACGTAGCTATAGAAACTGCAAGCCTCAAACATGTAGTCGGTGAGTTGGTGGCTATATTTTTCGAGTATAAAAAATTAG
CTGGACGATCTTCCATAGCC

Product: putative DNA polymerase III, gamma/tau subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSYIGLANKYRPRLLDEIVGQDFLVKCLSASIARDEVAGAILLSGPYGTGKTTTARAVTLSLLCLNRTSALPCLKCDSCI
SILSGSHPDILEIDAASNTGVEDVRMLIEGALYKPLLSKYKSYIVDEVHMLSQSAFSALLKLLEEPPQHVKFFLATTELQ
KIPATIISRCQHYRLSRLSRVVIAERLTQVAEKEQIEITQDAIKFIAEKADGSLRDALSLLEQVTLHPSEGKITVHVLRN
KMGLVDLSEVYMLLDSIIAGDCKTSLGKLNDLYSKGYEMLTIFMELVALINEKIQDKLTAEDSSKSGFFCRLAQCMLSSI
EEIKSFNCDHSVAELAIIRMSYIANLPSPGEIIKIFSNEMRTILPRNSEISKNSSMSSAELTSYILKEFDGSELSD

Sequences:

>Translated_396_residues
MSYIGLANKYRPRLLDEIVGQDFLVKCLSASIARDEVAGAILLSGPYGTGKTTTARAVTLSLLCLNRTSALPCLKCDSCI
SILSGSHPDILEIDAASNTGVEDVRMLIEGALYKPLLSKYKSYIVDEVHMLSQSAFSALLKLLEEPPQHVKFFLATTELQ
KIPATIISRCQHYRLSRLSRVVIAERLTQVAEKEQIEITQDAIKFIAEKADGSLRDALSLLEQVTLHPSEGKITVHVLRN
KMGLVDLSEVYMLLDSIIAGDCKTSLGKLNDLYSKGYEMLTIFMELVALINEKIQDKLTAEDSSKSGFFCRLAQCMLSSI
EEIKSFNCDHSVAELAIIRMSYIANLPSPGEIIKIFSNEMRTILPRNSEISKNSSMSSAELTSYILKEFDGSELSD
>Mature_395_residues
SYIGLANKYRPRLLDEIVGQDFLVKCLSASIARDEVAGAILLSGPYGTGKTTTARAVTLSLLCLNRTSALPCLKCDSCIS
ILSGSHPDILEIDAASNTGVEDVRMLIEGALYKPLLSKYKSYIVDEVHMLSQSAFSALLKLLEEPPQHVKFFLATTELQK
IPATIISRCQHYRLSRLSRVVIAERLTQVAEKEQIEITQDAIKFIAEKADGSLRDALSLLEQVTLHPSEGKITVHVLRNK
MGLVDLSEVYMLLDSIIAGDCKTSLGKLNDLYSKGYEMLTIFMELVALINEKIQDKLTAEDSSKSGFFCRLAQCMLSSIE
EIKSFNCDHSVAELAIIRMSYIANLPSPGEIIKIFSNEMRTILPRNSEISKNSSMSSAELTSYILKEFDGSELSD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI194306571, Length=284, Percent_Identity=25.3521126760563, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI194306567, Length=284, Percent_Identity=25.3521126760563, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI6677723, Length=281, Percent_Identity=25.6227758007117, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI31563534, Length=217, Percent_Identity=35.4838709677419, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI4506491, Length=303, Percent_Identity=25.0825082508251, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI31881687, Length=303, Percent_Identity=25.0825082508251, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI194306569, Length=200, Percent_Identity=26.5, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786676, Length=291, Percent_Identity=37.8006872852234, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17554730, Length=221, Percent_Identity=26.6968325791855, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986063, Length=236, Percent_Identity=26.271186440678, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17541988, Length=225, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324039, Length=290, Percent_Identity=25.8620689655172, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324478, Length=288, Percent_Identity=28.4722222222222, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6322528, Length=299, Percent_Identity=24.4147157190635, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319562, Length=245, Percent_Identity=27.3469387755102, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17647857, Length=226, Percent_Identity=30.0884955752212, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921076, Length=220, Percent_Identity=25.9090909090909, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI18859927, Length=227, Percent_Identity=28.1938325991189, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43777; Mature: 43646

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00242 INTEGRIN_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIGLANKYRPRLLDEIVGQDFLVKCLSASIARDEVAGAILLSGPYGTGKTTTARAVTL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
SLLCLNRTSALPCLKCDSCISILSGSHPDILEIDAASNTGVEDVRMLIEGALYKPLLSKY
HHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KSYIVDEVHMLSQSAFSALLKLLEEPPQHVKFFLATTELQKIPATIISRCQHYRLSRLSR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIAERLTQVAEKEQIEITQDAIKFIAEKADGSLRDALSLLEQVTLHPSEGKITVHVLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECC
KMGLVDLSEVYMLLDSIIAGDCKTSLGKLNDLYSKGYEMLTIFMELVALINEKIQDKLTA
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDSSKSGFFCRLAQCMLSSIEEIKSFNCDHSVAELAIIRMSYIANLPSPGEIIKIFSNEM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RTILPRNSEISKNSSMSSAELTSYILKEFDGSELSD
HHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYIGLANKYRPRLLDEIVGQDFLVKCLSASIARDEVAGAILLSGPYGTGKTTTARAVTL
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
SLLCLNRTSALPCLKCDSCISILSGSHPDILEIDAASNTGVEDVRMLIEGALYKPLLSKY
HHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KSYIVDEVHMLSQSAFSALLKLLEEPPQHVKFFLATTELQKIPATIISRCQHYRLSRLSR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIAERLTQVAEKEQIEITQDAIKFIAEKADGSLRDALSLLEQVTLHPSEGKITVHVLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEECC
KMGLVDLSEVYMLLDSIIAGDCKTSLGKLNDLYSKGYEMLTIFMELVALINEKIQDKLTA
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDSSKSGFFCRLAQCMLSSIEEIKSFNCDHSVAELAIIRMSYIANLPSPGEIIKIFSNEM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RTILPRNSEISKNSSMSSAELTSYILKEFDGSELSD
HHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]