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The map label for this gene is smf [H]

Identifier: 88608661

GI number: 88608661

Start: 317767

End: 318903

Strand: Reverse

Name: smf [H]

Synonym: NSE_0388

Alternate gene names: 88608661

Gene position: 318903-317767 (Counterclockwise)

Preceding gene: 88608339

Following gene: 88608784

Centisome position: 37.12

GC content: 40.37

Gene sequence:

>1137_bases
ATGATAATTAAGAATTTATCTAAAAGTGAACTGCTGTTCTGGCTACAGTTGTCTAGAACAGTTGGAGAAGTGACTTTTCA
GTCATTAATTAGCTTATATGGAACAGCTGAAAACGCTCTAGGACAACTTTCTACTATCCAACAACGTACAAAAAAAATAA
AACCTTTCTCCAAGACTGACGCACTAAAAGAGCTTGAGCTAACTGAAAAACTTGGCGCCAGTATTCTTGCCTCCTGCGAA
AACAAATATAGTAAAAACCTGAGAGTAATCAGAGATCCCCCTCCTGTACTTACAGTAAGAGGAAATATAGAACTGCTTAA
CACTCCTTCTGTAGCAGTAGTGGGCTCGAGAAATGCCACAATCAATAGTTGCAACTTTGCATATTCACTTGCGCGCGAAT
TAGCAGAAGAAGGATATACAATCGTTTCAGGCTTGGCTATAGGAGTAGACACAGCCGCATTAAAAATCACTGACAGCACA
CTACCAACAATCGCAGTAATAGGAACTGGTATAGATCAGTGTTATCCTATCGAAAATCAAAGATTACAAGAAAGAATTAT
CGAAAGAGGTGGCTTGGTGGTTACAGAAGTCCCTTTCGGTAAGCGAGGACAGTCTGTTCATTTTGCACGCAGAAACAGGA
TAATTTCAGGTCTTTCGCAGTGCGTTATCATTATTGAAGCATCAGAGAACTCTGGCTCTCTAATCACTGCCCAGTACGCA
CTGGCACAGGGCAAGTACCTCTTTGCATCACCGGGAGCACCATACGACAAAAGATTCTCGGGAAGTAATAGGCTCCTAAA
ACAGGGTGCCATATTGGTAGAAAATGCCTTAGACGTAATACAACACCTTAAAAAGAAAAAACGTAACGATTTTGGCTACA
AGACCCTTTTGGATGTTGAGGACAAGGAGTTTATACCTTTCTCCACGCTTAACTGTGACACCGATAAGATAACCGTAATA
AAGGCTCAGATCCTCAAAAAAATTGATAGTACATCTGTACCAATGAGTCAGTTAGCACATGAGCTTGGCATCTCAGAAAA
GACCCTCCTATTGGCAATAGTCGAACTTGAAATAGAGGGAAAAATAACACGGACCATAACAAATGAAGTTTCCATGCTTT
ATGATCAGTTTGCTTAA

Upstream 100 bases:

>100_bases
CAATATATGTCAGTTTTAGCTTATAGCGGTTAAAAAGAGCCTAACTACAAAAATTACATCCAAGGTTTACATCACAGGCA
CAATGTAATAGAGATATCTC

Downstream 100 bases:

>100_bases
CTCACTTAGTAAAATGGCACTCATCGGACTCTTAAGGCATATTACTTGGAAGAGTGGGTCTTTGTATGGAGAGCATAACT
GCTAAAGTGAAATCTCAAAA

Product: putative DNA processing protein DprA

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIIKNLSKSELLFWLQLSRTVGEVTFQSLISLYGTAENALGQLSTIQQRTKKIKPFSKTDALKELELTEKLGASILASCE
NKYSKNLRVIRDPPPVLTVRGNIELLNTPSVAVVGSRNATINSCNFAYSLARELAEEGYTIVSGLAIGVDTAALKITDST
LPTIAVIGTGIDQCYPIENQRLQERIIERGGLVVTEVPFGKRGQSVHFARRNRIISGLSQCVIIIEASENSGSLITAQYA
LAQGKYLFASPGAPYDKRFSGSNRLLKQGAILVENALDVIQHLKKKKRNDFGYKTLLDVEDKEFIPFSTLNCDTDKITVI
KAQILKKIDSTSVPMSQLAHELGISEKTLLLAIVELEIEGKITRTITNEVSMLYDQFA

Sequences:

>Translated_378_residues
MIIKNLSKSELLFWLQLSRTVGEVTFQSLISLYGTAENALGQLSTIQQRTKKIKPFSKTDALKELELTEKLGASILASCE
NKYSKNLRVIRDPPPVLTVRGNIELLNTPSVAVVGSRNATINSCNFAYSLARELAEEGYTIVSGLAIGVDTAALKITDST
LPTIAVIGTGIDQCYPIENQRLQERIIERGGLVVTEVPFGKRGQSVHFARRNRIISGLSQCVIIIEASENSGSLITAQYA
LAQGKYLFASPGAPYDKRFSGSNRLLKQGAILVENALDVIQHLKKKKRNDFGYKTLLDVEDKEFIPFSTLNCDTDKITVI
KAQILKKIDSTSVPMSQLAHELGISEKTLLLAIVELEIEGKITRTITNEVSMLYDQFA
>Mature_378_residues
MIIKNLSKSELLFWLQLSRTVGEVTFQSLISLYGTAENALGQLSTIQQRTKKIKPFSKTDALKELELTEKLGASILASCE
NKYSKNLRVIRDPPPVLTVRGNIELLNTPSVAVVGSRNATINSCNFAYSLARELAEEGYTIVSGLAIGVDTAALKITDST
LPTIAVIGTGIDQCYPIENQRLQERIIERGGLVVTEVPFGKRGQSVHFARRNRIISGLSQCVIIIEASENSGSLITAQYA
LAQGKYLFASPGAPYDKRFSGSNRLLKQGAILVENALDVIQHLKKKKRNDFGYKTLLDVEDKEFIPFSTLNCDTDKITVI
KAQILKKIDSTSVPMSQLAHELGISEKTLLLAIVELEIEGKITRTITNEVSMLYDQFA

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=320, Percent_Identity=30.625, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 41611; Mature: 41611

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKNLSKSELLFWLQLSRTVGEVTFQSLISLYGTAENALGQLSTIQQRTKKIKPFSKTD
CCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHH
ALKELELTEKLGASILASCENKYSKNLRVIRDPPPVLTVRGNIELLNTPSVAVVGSRNAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCEEEECCCCEEEEECCCCE
INSCNFAYSLARELAEEGYTIVSGLAIGVDTAALKITDSTLPTIAVIGTGIDQCYPIENQ
EECCHHHHHHHHHHHHCCCEEEEHEEEECCCEEEEEECCCCCEEEEEECCCHHCCCCCHH
RLQERIIERGGLVVTEVPFGKRGQSVHFARRNRIISGLSQCVIIIEASENSGSLITAQYA
HHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEH
LAQGKYLFASPGAPYDKRFSGSNRLLKQGAILVENALDVIQHLKKKKRNDFGYKTLLDVE
HHCCCEEEECCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
DKEFIPFSTLNCDTDKITVIKAQILKKIDSTSVPMSQLAHELGISEKTLLLAIVELEIEG
CCCCCCEEECCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECC
KITRTITNEVSMLYDQFA
EEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIKNLSKSELLFWLQLSRTVGEVTFQSLISLYGTAENALGQLSTIQQRTKKIKPFSKTD
CCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHH
ALKELELTEKLGASILASCENKYSKNLRVIRDPPPVLTVRGNIELLNTPSVAVVGSRNAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCEEEECCCCEEEEECCCCE
INSCNFAYSLARELAEEGYTIVSGLAIGVDTAALKITDSTLPTIAVIGTGIDQCYPIENQ
EECCHHHHHHHHHHHHCCCEEEEHEEEECCCEEEEEECCCCCEEEEEECCCHHCCCCCHH
RLQERIIERGGLVVTEVPFGKRGQSVHFARRNRIISGLSQCVIIIEASENSGSLITAQYA
HHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEH
LAQGKYLFASPGAPYDKRFSGSNRLLKQGAILVENALDVIQHLKKKKRNDFGYKTLLDVE
HHCCCEEEECCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
DKEFIPFSTLNCDTDKITVIKAQILKKIDSTSVPMSQLAHELGISEKTLLLAIVELEIEG
CCCCCCEEECCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEEEEECC
KITRTITNEVSMLYDQFA
EEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]